Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,676,343 G→C 8.5% Y62* (TAC→TAG ilvB2 ← pyruvate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,676,3430GC 8.5% 136.1 / 6.5 48Y62* (TAC→TAGilvB2pyruvate oxidase
Reads supporting (aligned to +/- strand):  ref base G (17/26);  new base C (2/2);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

GCTGTACTATTCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTCCTGGAATACCATACAAGTGATCTA  >  CP000730/2676206‑2676473
                                                                                                                                         |                                                                                                                                  
gCTGTACTATTCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTaa                                                                                                                                 >  1:75396/1‑141 (MQ=255)
   gTACTATTCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCCCACCGATTTTACCAGTTAATTTTTTTTAAcaa                                                                                                                              >  2:8239/1‑139 (MQ=255)
   gTACTATTCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACca                                                                                                                              >  2:302892/1‑141 (MQ=255)
   gTACTATTCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACca                                                                                                                              >  1:113738/1‑141 (MQ=255)
       tATTCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACcagcag                                                                                                                          <  1:341169/141‑1 (MQ=255)
        aTTCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCagcagc                                                                                                                         >  1:239815/1‑141 (MQ=255)
          tCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCg                                                                                                                       <  1:103084/141‑1 (MQ=255)
          tCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCg                                                                                                                       <  2:215430/141‑1 (MQ=255)
          tCGTCTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATATCATTTAATAAATGAATTCAACCTGTGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCg                                                                                                                       <  2:59923/141‑1 (MQ=255)
           cGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGc                                                                                                                      <  2:178396/141‑1 (MQ=255)
            gTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAGTTTTGTCTAACCAGCAGCCGcc                                                                                                                     >  2:141799/1‑140 (MQ=255)
            gTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTCTAACCAGCAGCCGCt                                                                                                                     >  2:141808/1‑141 (MQ=255)
              ttGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTaa                                                                                                                   >  1:361720/1‑141 (MQ=255)
                   cAGATAATATTAATTACGGTACATTGTCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTg                                                                                                              >  1:186098/1‑141 (MQ=255)
                    aGAAAATATTAATTGCCGTACATTACCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGc                                                                                                             <  1:20185/141‑1 (MQ=255)
                     gATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCt                                                                                                            <  2:59025/141‑1 (MQ=255)
                     aaaaataTTAATTGCGGTACATTATTCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGTGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTCCt                                                                                                            <  2:362456/138‑1 (MQ=255)
                              taTTGCGGTACCTTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACCCGGGTTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCa                                                                                                   <  2:33709/140‑1 (MQ=255)
                                      tACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTAc                                                                                           <  1:337507/141‑1 (MQ=255)
                                      tACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTAc                                                                                           <  2:357243/141‑1 (MQ=255)
                                                  tttGGCAGCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATTCTTAATGCCACCCCGATTTTACCAGTTCATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACCTACACGATAACAttt                                                                               <  2:165060/141‑1 (MQ=255)
                                                  tggtgCATCAGACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAAttt                                                                               <  2:182583/137‑1 (MQ=255)
                                                                      ttAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTCATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTaa                                                           >  2:26344/1‑140 (MQ=255)
                                                                       gactaaCTGAATTACACGAGGGCCCCCGATACTTCATGCCAAACCGATCTTACCAGTTAATTTTGTGTAACCAGCACTCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACg                                                          <  2:101781/138‑1 (MQ=255)
                                                                         ataaATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTa                                                        <  2:244331/141‑1 (MQ=255)
                                                                          taaATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTCTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTaa                                                       <  1:141808/141‑1 (MQ=255)
                                                                          taaATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGGTAAGTTTGTCTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTaa                                                       <  1:141799/141‑1 (MQ=255)
                                                                           aaaTGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATGTTACCAGTTAATTTTGTGTAACCATCAGCCGATACGATCGTCACGTGCCCACTCCGTGCGTCATAAACTTTAAATTGATCTCTCACCTTAAGTaaa                                                      >  2:262885/1‑141 (MQ=255)
                                                                             aTGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACt                                                    <  1:181424/141‑1 (MQ=255)
                                                                                   tAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGCACATGATAAAATTTAAATTGATCTCTCACTGTACGTATACTATCGac                                              <  2:239815/141‑1 (MQ=255)
                                                                                    aaaCCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTTTGTAACCAGCAGCCGCTAAACTTGCTACTTCTTCATTACGTAAATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGact                                             >  2:103102/1‑141 (MQ=255)
                                                                                         aGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTCCGTAAACTATCGACTACTGc                                        <  2:361720/141‑1 (MQ=255)
                                                                                          gggCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATTACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCg                                       >  2:16027/1‑141 (MQ=255)
                                                                                              cACCGATACTTAATGCCACACCGAATTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCGCTGTACGTAAACTATCGactaccaccgcaa                                   >  2:28954/1‑133 (MQ=255)
                                                                                               aCCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAAATTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGAt                                  >  1:339344/1‑141 (MQ=255)
                                                                                                  gATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCATCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGa                               <  2:291013/141‑1 (MQ=255)
                                                                                                       ttAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACCGAACGCAAACTATCGACCACCGCGTCGATTTAAtctc                          >  2:310052/1‑141 (MQ=255)
                                                                                                         aaTGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTCCt                        <  1:304871/141‑1 (MQ=255)
                                                                                                          attccACCCCGTTTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTCCTg                       <  2:161569/138‑1 (MQ=255)
                                                                                                              cacaCCGATTTTACCAGTTAATTTTGTGTAACCACCAGCAGCTAATCTTGGTACGTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAACCTCTCGCCTCCTGCGTCGATTGAGTCTCCTgcact                   >  2:280565/1‑137 (MQ=255)
                                                                                                                  ccGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCCCCGTACGTAAACTATCGACTACTGCGTCGCTTGAGTCTCCTGGACTAccc               >  1:209405/1‑140 (MQ=255)
                                                                                                                  ccGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTTCTGGAATACCa               >  1:241193/1‑141 (MQ=255)
                                                                                                                   cGATTTGACCAGTTAATTTGGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCTACTACTGCGTCGATTGAGTCTCCTGGAATACCAt              <  2:183638/141‑1 (MQ=255)
                                                                                                                     tttgtACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCCTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTCCTGGAATACCATAc            <  1:107403/137‑1 (MQ=255)
                                                                                                                      ttttACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGTGGGGTTTGAGTCTCCTGGAATACCATACa           <  1:180177/141‑1 (MQ=255)
                                                                                                                        ttACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTCCTGGAATACCATACAAg         >  1:41265/1‑141 (MQ=255)
                                                                                                                          aCCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTCCTGGAATACCATACAAGTg       <  1:59346/141‑1 (MQ=255)
                                                                                                                               ttAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTCCTGGAATACCATACAAGTGATCta  <  1:268591/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
GCTGTACTATTCGTTTGTCCAGATAATATTAATTGCGGTACATTATCCATTTTGGCATCATACATACCATTTAATAAATGAATTAAACCAGGGCCACCGATACTTAATGCCACACCGATTTTACCAGTTAATTTTGTGTAACCAGCAGCCGCTAAGCTTGCTACTTCTTCATGACGTACATGATAAAATTTAAATTGATCTCTCACTGTACGTAAACTATCGACTACTGCGTCGATTGAGTCTCCTGGAATACCATACAAGTGATCTA  >  CP000730/2676206‑2676473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: