BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,970	T→C	55.8%	E194E (GAA→GAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,970	0	T	C	55.8%	122.8 / 299.7	254	E194E (GAA→GAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (55/56); new base C (63/76); total (122/132)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 256 total aligned reads displayed.

GCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAG  >  CP000730/35845‑36109

cacaaacacgtatatATCATGTCACTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTaaa                                                                                                                              <  2:356521/127‑1 (MQ=1)

  taatcccTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGt                                                                                                                            <  1:102662/135‑1 (MQ=1)

           atatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTTATTTCTTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGc                                                                                                                   >  1:352814/4‑141 (MQ=1)

                       aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                                                       >  1:325465/1‑141 (MQ=255)

                        gttttCGTTTTACTTGCTAGCATGATGCTAATTTCGAGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                      <  1:220734/138‑1 (MQ=11)

                        gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGCGGATACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                      <  1:368075/141‑1 (MQ=11)

                         tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                                                     >  1:249908/1‑141 (MQ=255)

                           ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAACATCCGCAGGTGTGAAAAGGCCTGCGGTTCTTATTATATCAGA‑CACCAAAACACTCCATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                   >  2:113727/1‑141 (MQ=11)

                           ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGAGTAAATAGACTAACTACTTTTTAAAGTTATCTTTACTGTATTGATCCTTTgc                                                                                                   >  2:240599/1‑140 (MQ=11)

                            tCGTTTAACTTGCTTGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                  <  2:370183/141‑1 (MQ=255)

                            tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                  >  1:74246/1‑141 (MQ=17)

                             cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                                                                                 >  2:139994/4‑141 (MQ=255)

                             cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                                                 <  1:193857/138‑1 (MQ=17)

                              gtttAACTTTCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTCATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATCGATACTTTGATat                                                                                                <  2:157458/139‑2 (MQ=11)

                              gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCCATGCCTGTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                >  1:287963/1‑141 (MQ=255)

                              gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                <  2:365020/141‑1 (MQ=17)

                              gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                <  2:49348/141‑1 (MQ=17)

                               gttAACTTTCTAGCATGATGTGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                               <  2:317807/140‑1 (MQ=255)

                                ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAGCCCTGAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                                                                              <  1:288064/141‑4 (MQ=11)

                                 tAACTTGCCAGCATGTTGCTAATTTCGTGGCATGTGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑ATCGCGTAAATACGTTCAATACCTTTTAACGTATTCTTTGCCTTATTGATACTTTGATATCtt                                                                                             <  2:164765/141‑1 (MQ=11)

                                   aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                                                           >  1:234086/1‑141 (MQ=17)

                                    cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATTTCTTGtc                                                                                          >  2:214842/1‑141 (MQ=11)

                                     ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                         <  2:64471/141‑1 (MQ=17)

                                       gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                                                                                       <  1:45719/141‑1 (MQ=11)

                                       gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                       <  2:262409/141‑1 (MQ=17)

                                       gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGtcttt                                                                                       >  1:10176/1‑141 (MQ=17)

                                        cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtatttc                                                                                      >  2:247793/1‑141 (MQ=11)

                                         tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                     <  1:322165/141‑1 (MQ=32)

                                         tAGCATGATGCTAATTTCGTGGCATGAGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATAGATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                                                     <  1:227971/141‑1 (MQ=11)

                                          aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttactt                                                                                    >  2:250530/1‑141 (MQ=17)

                                              tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                >  1:354009/1‑141 (MQ=255)

                                               gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATATCAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                               >  2:32939/1‑141 (MQ=255)

                                                 tgCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATTT‑AGAGCGGAAATACACCCAATACCTTTTAAAGTATTCTTTGCTGTTTTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                             >  1:131354/1‑141 (MQ=11)

                                                 tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                             <  1:75992/141‑1 (MQ=21)

                                                  gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAat                                                                            >  1:64606/1‑141 (MQ=11)

                                                  gCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTatt                                                                            >  2:230869/1‑139 (MQ=19)

                                                   cTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                                                           <  1:40514/141‑1 (MQ=255)

                                                   cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                           >  2:269327/1‑141 (MQ=21)

                                                   cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATAAACTCAATACCTTTTAAAGTATACTTTGCTGTATTGTTACTTTGATACCTTGTCTTTCTTACTTTAatt                                                                           >  2:340586/1‑140 (MQ=255)

                                                     aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTTATATCTTGTCTTTCTTACTTTAATATg                                                                         >  1:304752/1‑141 (MQ=11)

                                                       tttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAc                                                                       <  1:359891/141‑1 (MQ=11)

                                                        ttCGTGGCATGGGCAAAGTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTTCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                                                      <  1:25851/141‑1 (MQ=11)

                                                         tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                                                     <  1:48683/141‑1 (MQ=38)

                                                          cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                    <  1:266827/141‑1 (MQ=255)

                                                           gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                                                   >  1:231267/1‑141 (MQ=14)

                                                           gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                   <  1:239946/141‑1 (MQ=255)

                                                            tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                  >  1:97956/1‑141 (MQ=255)

                                                            tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                  >  2:303568/1‑141 (MQ=255)

                                                            gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                  <  2:334242/140‑1 (MQ=255)

                                                              gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                <  1:210740/141‑1 (MQ=255)

                                                                  ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTATTTATAC‑AGTGCGCAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                            <  1:29009/138‑1 (MQ=14)

                                                                      aaaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                                                        <  1:111954/141‑1 (MQ=255)

                                                                        aaTCCGTAGATCTGAAGAGAACTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTGTCTTACTGTAATATGACGATGATCTTGCTCAATg                                                      >  1:225728/1‑141 (MQ=255)

                                                                         aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATGCTTTGATCTCTTGTCTTCCTTCCTCTCATCTATCGGTGATCTTGCTCAATGa                                                     >  1:67203/1‑141 (MQ=11)

                                                                         aTCCGTAGATCTGAAGAGACCTACGGATCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTACTATGACGGTGATCTAGCTCAATGa                                                     <  1:273391/141‑1 (MQ=14)

                                                                          tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                    <  2:276054/141‑1 (MQ=255)

                                                                            cGTAGATCTGAAGAGACCTGCTGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                  <  2:224273/141‑1 (MQ=255)

                                                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                  >  1:97327/1‑141 (MQ=255)

                                                                                   cTGAAGAGACCTACGGCTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                           >  1:185131/1‑141 (MQ=255)

                                                                                    tGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                          <  1:53844/141‑1 (MQ=255)

                                                                                    ggAAGAGACCTACGGTTCTTTTTATAT‑AGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCCTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                          <  1:12654/140‑1 (MQ=11)

                                                                                     gaggagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                         <  2:190055/138‑1 (MQ=255)

                                                                                     gAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                         >  1:276089/1‑141 (MQ=38)

                                                                                        gagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                      <  2:284436/141‑1 (MQ=38)

                                                                                        gagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                      <  1:186587/141‑1 (MQ=255)

                                                                                         agaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                     <  2:360413/141‑1 (MQ=255)

                                                                                           aactacGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTATTTTAATATGACGGTGATGTTGCTGCATGAGGTTATTCAGATAtttgt                                   >  1:153147/6‑139 (MQ=11)

                                                                                           aCCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                   <  2:148816/141‑1 (MQ=255)

                                                                                           aCCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                   <  2:232153/141‑1 (MQ=255)

                                                                                            ccTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                  >  2:321417/1‑141 (MQ=38)

                                                                                            ccTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                  <  2:118876/141‑1 (MQ=255)

                                                                                            ccTACGCTTCTTTTTATAT‑AGCGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTCATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                  <  1:184784/141‑1 (MQ=11)

                                                                                             cTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTCAAGTTTCATGTAGGTTATTTATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                 <  2:262933/141‑1 (MQ=14)

                                                                                             cTGCGGTTCTTTTTATAT‑AGAGCGTAAATAAACTCAATACCTTTTAAAGTATTCTTTGCGGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                 >  2:217246/1‑141 (MQ=255)

                                                                                               gCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                               >  2:228350/1‑141 (MQ=38)

                                                                                               acGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                               <  1:330142/140‑1 (MQ=255)

                                                                                                    tttttttATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATCACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                          <  2:243296/139‑1 (MQ=255)

                                                                                                    tCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                          <  2:345217/141‑1 (MQ=255)

                                                                                                     cTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCGGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TATTCAGATATTTCGATGTACAATgg                        >  1:9167/1‑140 (MQ=255)

                                                                                                      tttttATAT‑AGAGCGTAAAAACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTCATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                        >  1:278716/1‑140 (MQ=255)

                                                                                                      tttttATAT‑AGAGAGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCACTGAGGGTATTCGGATATTTCGATGTACCATGa                        >  2:304549/1‑141 (MQ=14)

                                                                                                       ttttATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggc                       >  2:142530/1‑139 (MQ=255)

                                                                                                        tttATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATTACGGTGATCTTGCTCAATGAGGTTATTCAGATTTTTCTATTTACAATGACa                      >  1:247739/1‑141 (MQ=14)

                                                                                                          tatat‑aGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                    <  2:202701/141‑1 (MQ=255)

                                                                                                           atat‑aGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTc                   >  2:237799/1‑141 (MQ=255)

                                                                                                           atat‑aGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAGTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGtt                   >  1:271817/1‑140 (MQ=255)

                                                                                                            tat‑aGAGCCTAAATCCTCTCAATACTTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCa                  <  1:142164/141‑1 (MQ=14)

                                                                                                              t‑aGTGCGTAAATACATTCAATACCTGTTAAAGTATTCTTGGCCGTAGTGATACGTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCGTGCTCTATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                <  2:184969/141‑1 (MQ=14)

                                                                                                                agagGGTAAGTACACTCAATACCTTTTATAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCCTACTTTAATATGCCGGTGCTCTCGCTTAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgt               <  2:170228/141‑1 (MQ=14)

                                                                                                                agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATCCTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgt               >  1:311193/1‑141 (MQ=255)

                                                                                                                   gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTAATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttta            <  1:10712/141‑1 (MQ=255)

                                                                                                                     tgaaaTACCTTCAATACCTTTTAAAGTATTCTTGGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag          <  1:83207/139‑1 (MQ=255)

                                                                                                                     gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag          <  1:19849/141‑1 (MQ=255)

                                                                                                                      tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt         <  2:363276/141‑1 (MQ=255)

                                                                                                                         aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGCTATTTCGTTGTACAATGGCAATCAGctctaagctta      >  1:175132/1‑141 (MQ=14)

                                                                                                                         aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta      >  1:138473/1‑141 (MQ=255)

                                                                                                                          tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa     >  2:130157/1‑141 (MQ=255)

                                                                                                                          tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaggtttaa     >  1:335553/1‑141 (MQ=255)

                                                                                                                          tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAACTTGTATTTCTTCCTTTAATATGACGGTGATATTGCTCAGCGCAGATCATCCGTTATTTCGATGTACAATGGCAGGAAGgtttaagtttaa     >  2:78013/1‑141 (MQ=14)

                                                                                                                            cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa   <  1:243759/141‑1 (MQ=255)

GCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAG  >  CP000730/35845‑36109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`