Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,814 Δ1 bp 32.8% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8140A.32.8% 258.5 / 164.2 131intergenic (+45/+63)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (45/42);  new base . (12/31);  total (57/74)
Fisher's exact test for biased strand distribution p-value = 1.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01

Only 100 of 131 total aligned reads displayed.

ATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTT  >  CP000730/35675‑35951
                                                                                                                                           |                                                                                                                                         
  gCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGaa                                                                                                                                        >  2:437220/1‑141 (MQ=255)
     aaTTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGt                                                                                                                                     <  1:267187/141‑1 (MQ=255)
        tattCTGAATTTAAAGCTGTAGATGATCGCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCt                                                                                                                                  <  1:212650/141‑1 (MQ=255)
         attCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCtt                                                                                                                                 >  1:176369/1‑140 (MQ=255)
         attCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTg                                                                                                                                 >  1:593046/1‑141 (MQ=255)
          ttCTGAATTTAAAGGTGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGTAAAATGATTACTAGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTATGt                                                                                                                                >  2:540370/1‑141 (MQ=255)
          ttCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGt                                                                                                                                >  1:629168/1‑141 (MQ=255)
            cTGAATTTAAAGATGAAGATGATCTCATTGACAAAGTATTTACTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTg                                                                                                                              >  1:398652/1‑141 (MQ=255)
             tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGc                                                                                                                             <  1:361908/141‑1 (MQ=255)
                   tAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTa                                                                                                                       >  2:487632/1‑141 (MQ=255)
                     aaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaa                                                                                                                     >  1:192642/1‑141 (MQ=255)
                     aaGATGTAGATGATCTCAGTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaa                                                                                                                     <  2:105283/141‑1 (MQ=255)
                      aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                    >  2:475200/1‑141 (MQ=255)
                      aGATGTAGATGATCTCATTGACAAAGTATCTAATGTAGAGGCTATCAAGTATACAGATTTCGGCGAAAACTGATTCCTTGAACAAGAGCTCAAATAAGATTGCAATCTAATAAAACTAGAAGGTGCTGTTGCAAATTaaaa                                                                                                                    >  2:733759/1‑141 (MQ=255)
                      aGATGCAGATGATCTATAAAACAAAGTATTTAATGTAGTAGCAATTCAGGATAAAGTCTTTGGTGGTACATGATTAATTGATTTAGAATTAAACAAATATATTGATGTAATAAAAATAGAAGGTTCTGTGGCAAAGTtaaa                                                                                                                    >  2:275902/1‑141 (MQ=255)
                       gATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaaa                                                                                                                   <  1:641525/141‑1 (MQ=255)
                        aTGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaaaa                                                                                                                  >  1:507431/1‑141 (MQ=255)
                            aGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAatat                                                                                                              <  1:385741/141‑1 (MQ=255)
                             gatgatCTCATTGAAAAAGTATTTAATGTAGAAGCTACTCTGTTTCTTGTTCTTCCGGGTTAAAAAGTACTTGCAGACCAGCTAAAACAAAATATAAATCTAATAAAAATAGAAGGTTCTGAAAAAAAAAAAAAAAAtata                                                                                                             <  2:166054/141‑1 (MQ=255)
                             aatgattaaaTTAACAAAATATTTAATGTAAAAGCTATCTAATATAAAATTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAtata                                                                                                             <  2:151050/132‑1 (MQ=255)
                              atgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAg                                                                                                            <  1:96373/141‑1 (MQ=255)
                              atgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAg                                                                                                            <  1:136977/141‑1 (MQ=255)
                                     cATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCa                                                                                                     >  2:710398/1‑141 (MQ=255)
                                      aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                    >  1:322350/1‑141 (MQ=255)
                                      aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                    >  1:328275/1‑141 (MQ=255)
                                      aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                    >  1:773262/1‑141 (MQ=255)
                                       ttGACAAAGTATTTAATGTAGAAGCTAGTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACt                                                                                                   <  2:306001/141‑1 (MQ=255)
                                            aaaGTAATTAAGCTTGAAGCTATTCAGTATAAAGGTGTCGTCCAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAACTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAttt                                                                                              <  1:163459/141‑1 (MQ=37)
                                              aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTTAACGAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                            >  1:183015/1‑141 (MQ=255)
                                              aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                            >  1:348197/1‑141 (MQ=255)
                                               gTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATc                                                                                           >  1:502719/1‑141 (MQ=255)
                                                 aTTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCAt                                                                                         <  1:174987/141‑1 (MQ=255)
                                                     aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTAATTGAACAGGAGCTAAAAGAAAATTGTGATCTAATAAAATTAGAAGGTTCTGTTGCAAAGTAAAAGAATATAGCTAACAACTAATTTCTCTTGTCa                                                                                     >  2:384404/1‑141 (MQ=38)
                                                     aaTGTAGAAGCTATTCAAGATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAATATTGTGATCTAATAAAAATAGAAGGTTATGTTGCAAAATAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                     >  2:182925/1‑141 (MQ=38)
                                                         tAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                 <  1:184733/141‑1 (MQ=255)
                                                             atcTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                             <  2:410365/139‑1 (MQ=255)
                                                             aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                             <  2:144333/141‑1 (MQ=255)
                                                               cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                           >  1:432990/1‑141 (MQ=255)
                                                               cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                           >  2:417709/1‑141 (MQ=255)
                                                                 aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                         >  1:675726/1‑141 (MQ=255)
                                                                   tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                       >  1:307313/1‑141 (MQ=255)
                                                                    taGTATAAAGTTTTTGGCGAAAAATGATTACTTGAATAAGATTTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                      <  2:80172/140‑1 (MQ=255)
                                                                    cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                      >  2:718520/1‑141 (MQ=255)
                                                                      gTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                    >  1:546589/1‑141 (MQ=255)
                                                                       tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTCCTGTTGCAAAGTACAAAAATATAGCTAACAACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                   >  2:255557/1‑141 (MQ=255)
                                                                         taAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTCACTTGCTAg                                                                 >  1:772572/1‑141 (MQ=255)
                                                                                tttGGCGAAAAAACATTACGTGAACAAGAGCTAAAATAAAGTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                          <  1:424521/141‑1 (MQ=255)
                                                                                    ttttttttttaTTACTTGAACAAGAGCTAAAATACAATTGGGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAGATATCTAACCACTAATTTATCATCTCAGTGTTCACTTAACTTGCTACCATGATGCTaa                                                      >  2:518972/11‑141 (MQ=18)
                                                                                     cGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAAt                                                     >  2:376201/1‑141 (MQ=255)
                                                                                           aTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                               <  1:543815/141‑1 (MQ=255)
                                                                                                   ttGAATCAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCg                                       <  1:1260/141‑1 (MQ=255)
                                                                                                          agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                <  2:675726/141‑1 (MQ=255)
                                                                                                                   acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTCATTTGCTTGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                     >  1:570813/15‑141 (MQ=11)
                                                                                                                   acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                     >  1:67117/15‑141 (MQ=11)
                                                                                                                   acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                     >  2:686909/15‑141 (MQ=11)
                                                                                                                    caaaagagtatcttaataaAAATGAGGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                    <  1:690303/128‑1 (MQ=11)
                                                                                                                      aaatTGACATCTAATAAAAATAGAATGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                    <  1:474082/141‑1 (MQ=255)
                                                                                                                      aaagactattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTCTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                  <  1:378872/130‑1 (MQ=11)
                                                                                                                       aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATCTGAAgaga                 >  1:477332/11‑141 (MQ=11)
                                                                                                                        agagtattttaataaAAATTGTGGTTCTGTTGCCAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                <  2:10018/132‑1 (MQ=11)
                                                                                                                        agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTGATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGAAATTCTGAAAATCCGTAGATCTGAAGAGAc                <  2:522131/132‑1 (MQ=11)
                                                                                                                        agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                <  1:11685/132‑1 (MQ=11)
                                                                                                                        agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                <  1:419643/132‑1 (MQ=11)
                                                                                                                        agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                <  2:150579/132‑1 (MQ=11)
                                                                                                                        agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                <  2:780424/132‑1 (MQ=11)
                                                                                                                         gattattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc               <  1:251949/133‑1 (MQ=11)
                                                                                                                         gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc               <  2:93793/133‑1 (MQ=11)
                                                                                                                         gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc               <  1:365719/133‑1 (MQ=11)
                                                                                                                         gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc               <  1:192050/133‑1 (MQ=11)
                                                                                                                          tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                <  1:329543/141‑1 (MQ=255)
                                                                                                                          tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                <  1:122288/141‑1 (MQ=255)
                                                                                                                          tgtgAATTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                <  1:712039/141‑1 (MQ=255)
                                                                                                                          ggtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGCAAAAACATATAGCTAACCACTAATTTATCATGTCAGTCTTCGCTTAACTTGCTAGCATGATGCTAATCTCGTGGCATGGCGAAAATCCGTGAATCTGCAGCGAc                >  2:588483/2‑141 (MQ=14)
                                                                                                                          agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGGTCTTAGGAGACCt              >  2:509622/8‑141 (MQ=11)
                                                                                                                          agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt              <  2:572424/134‑1 (MQ=11)
                                                                                                                          agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt              <  2:572279/134‑1 (MQ=11)
                                                                                                                          agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt              <  2:568759/134‑1 (MQ=11)
                                                                                                                          agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt              <  2:439740/134‑1 (MQ=11)
                                                                                                                          agtattttaataaAAATGATGGTTCTGGTGCAAAGTAAAAAATATAGCTAACCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt              <  2:143336/134‑1 (MQ=11)
                                                                                                                          agtattttaataaAAATGATAGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt              >  1:476811/8‑141 (MQ=11)
                                                                                                                           gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc               <  2:87037/141‑1 (MQ=255)
                                                                                                                           gtattttgataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg             <  2:751393/133‑1 (MQ=11)
                                                                                                                           gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg             <  2:311450/135‑1 (MQ=11)
                                                                                                                           gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg             <  1:563105/135‑1 (MQ=11)
                                                                                                                           gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCAGTTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTg             >  1:380189/7‑141 (MQ=11)
                                                                                                                            tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt              <  2:244772/141‑1 (MQ=255)
                                                                                                                            tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc            <  1:702764/136‑1 (MQ=11)
                                                                                                                            tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc            >  2:415452/6‑141 (MQ=11)
                                                                                                                            tattttaagaaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCCAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc            <  1:224420/136‑1 (MQ=11)
                                                                                                                              ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTGACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAtgaggactgagg          >  1:58427/4‑133 (MQ=11)
                                                                                                                              ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTCCTAGCATGATGCTAATTTCGTGGCATGGGGAAAACCCGTACATCTGAAGAGAACTGAgg          >  2:195721/4‑141 (MQ=11)
                                                                                                                               tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATGTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt         <  1:85120/139‑1 (MQ=11)
                                                                                                                               tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt         <  2:770750/139‑1 (MQ=11)
                                                                                                                               tttaataaAAATGATGGTGCTGTTGCAAAGTAAAAAATATAGCTCATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt         <  2:687952/139‑1 (MQ=11)
                                                                                                                                cTAATAAAAATAGAAGGTTCTGTAACAAAAAAAAAAAATATAGCTAACCACTATTTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg          <  2:435088/141‑1 (MQ=14)
                                                                                                                                ataataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg          <  2:397519/140‑1 (MQ=40)
                                                                                                                                 taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGCTCTGAAGAGACCTGCGGTTc       <  1:510990/141‑1 (MQ=11)
                                                                                                                                     aaaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATGTGAATAACCACTTATTACAGATTCCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt     >  1:186228/1‑141 (MQ=11)
                                                                                                                                      aaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt    <  2:307313/141‑1 (MQ=35)
                                                                                                                                        aaTAGGAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCGGTAGTGCTGAAGAGTCCTGCGGTTCttttt  <  2:398652/141‑1 (MQ=11)
                                                                                                                                           |                                                                                                                                         
ATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTT  >  CP000730/35675‑35951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: