Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,015 C→T 29.4% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0150CT29.4% 1440.7 / inf 1043R179R (AGG→AGAUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (390/338);  new base T (157/150);  total (552/491)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1072 total aligned reads displayed.

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155
                                                                                                                                            |                                                                                                                                            
ttttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACACAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                                              <  1:433902/139‑1 (MQ=12)
  ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcat                                                                                                                                            >  2:693774/1‑139 (MQ=17)
   tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAAGCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGTTACTTTGATATCtt                                                                                                                                           >  2:434486/1‑141 (MQ=11)
   tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                                           >  2:571044/1‑141 (MQ=17)
    aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGCGCGTAAATACCTTCAATACCTTTTAACGTATTCTTTGCTGTATTGTTACTTTCATCCCTTg                                                                                                                                          >  1:355226/1‑141 (MQ=11)
    aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                                                                          >  2:666264/1‑141 (MQ=17)
     aCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGATTAAACCTACGGTTCTTCTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGt                                                                                                                                         <  1:149241/141‑1 (MQ=11)
      cTTGGTAGCATGGTGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                                        <  1:687123/141‑1 (MQ=12)
      cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                                        >  1:310238/1‑141 (MQ=32)
       ttGCTAGCATGATGCTAATTTCGTGGCTTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                                       <  1:649132/141‑1 (MQ=17)
         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                     <  1:226505/141‑1 (MQ=17)
         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                     <  1:41849/141‑1 (MQ=17)
          cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTCTTGATACTTTGATACATTGtctttc                                                                                                                                    >  2:32286/1‑141 (MQ=12)
           tAGCATGATGCTAATTTCGTTGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                                   <  1:101260/141‑1 (MQ=17)
           tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                                                                                                   <  2:31134/141‑1 (MQ=255)
            aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGACCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                                                                  <  2:13021/141‑1 (MQ=17)
               atgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACt                                                                                                                               >  2:787238/1‑141 (MQ=11)
                tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                                                                              >  2:622483/1‑141 (MQ=25)
                ggatgCTAATTTCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                                                                              <  2:216715/140‑1 (MQ=255)
                 gatgCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                                             <  1:675198/141‑1 (MQ=255)
                 cagccTACTTTCGTGGCATGATGAAAATACTTGGATCTTATCACAAGTGCCGGTCTCCTGATACAGAGCGTAAATACATTTGATTTCTTTTACAGCATTCTTTGCTGTATTGATACTTTTATACCTTTTCTTTCTTAAttg                                                                                                                             <  2:111371/137‑2 (MQ=255)
                   tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                                                                           <  1:113853/141‑1 (MQ=255)
                   tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                                                                           <  2:596817/141‑1 (MQ=25)
                   tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGATCTTTGTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                                                                           >  1:186727/1‑141 (MQ=255)
                   tgCGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTAGATACCTTGTCTTTCTTACTTTaa                                                                                                                           >  2:494199/1‑141 (MQ=255)
                    gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                                                                                                          <  2:587995/141‑1 (MQ=25)
                    gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAat                                                                                                                          >  2:54013/1‑141 (MQ=255)
                       aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                                       <  2:509119/141‑1 (MQ=25)
                        aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTCAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                                                                                                                      >  2:86185/1‑141 (MQ=25)
                           tCGTGGCATGGTGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACgg                                                                                                                   <  1:26805/141‑1 (MQ=255)
                            cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTACTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                                  >  2:352038/1‑141 (MQ=255)
                             gTGGCATGGGGAAAATCCGTATATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTACTCTTTTCCGTATCGATACTTTGATATCCTTTCTTTCTTACTTTTAGATCACGGTg                                                                                                                 >  1:127634/1‑141 (MQ=11)
                             gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 >  1:58175/1‑141 (MQ=11)
                             gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 >  2:284112/1‑141 (MQ=38)
                                gCATGGGGAAAATCCGTTGATATGAAGAGTCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                                              <  1:709320/141‑1 (MQ=11)
                                gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                                              >  1:533059/1‑141 (MQ=255)
                                gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                                                                              <  1:609307/141‑1 (MQ=255)
                                     gCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                                         <  1:243827/141‑1 (MQ=255)
                                     gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                                         >  1:492080/1‑141 (MQ=255)
                                      cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                                                                        >  1:624641/1‑141 (MQ=37)
                                      cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                                                                        >  1:780039/1‑141 (MQ=255)
                                       gAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATCTGACGGTGATATTGCTCa                                                                                                       >  2:161748/1‑141 (MQ=255)
                                        aaaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                                                                                                      <  2:396488/141‑1 (MQ=255)
                                         aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     >  2:526936/1‑141 (MQ=255)
                                          aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                                                                    <  1:746154/141‑1 (MQ=255)
                                            tCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAGCTTGCTCAATGAg                                                                                                  >  1:82974/1‑141 (MQ=255)
                                              cGTAGGTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAGGTATTCTTGGCTGTATGGCTAGTTTGATACCTTGTCTTGCTTACTTTAATATGACGGTGATCTGGCTCAATGAGGt                                                                                                <  2:29997/141‑1 (MQ=11)
                                              cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCACAATGAGGt                                                                                                >  2:14526/1‑141 (MQ=255)
                                               gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCATTGTGGtt                                                                                               >  2:546761/1‑141 (MQ=255)
                                                        aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTTTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACAGTGATCTTGCTCAATGAGGTTATTTAGatt                                                                                      >  1:321893/1‑140 (MQ=11)
                                                        aaGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                                                                      <  1:281606/141‑1 (MQ=255)
                                                          ggtgccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTCCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                                    <  2:3814/137‑1 (MQ=255)
                                                            gcgcTGTGGGTCTTTTTATATAGAGCGCAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                                                                  <  1:733759/138‑1 (MQ=255)
                                                            gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                                                                  >  1:39092/1‑141 (MQ=38)
                                                             aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                                                 >  2:467257/1‑141 (MQ=255)
                                                                tacGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGATg                                                                              >  2:422772/3‑141 (MQ=255)
                                                                 acGGTTCCTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCGTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                             <  1:40226/140‑1 (MQ=255)
                                                                  cGGTTCTTTGTATATAGAGCGTAAATACATTCAATATCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATATCGTGTCTTTCTTACTGTGATTTGACACTGATCTTGCGCTAGGAGGCTATTCAGATATTTCGATGTa                                                                            <  2:362781/141‑1 (MQ=11)
                                                                      tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATTTACAAt                                                                        <  2:71515/141‑1 (MQ=255)
                                                                           ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACAATGACAg                                                                   >  1:664920/1‑141 (MQ=255)
                                                                               ttatatcttAAATACACTCAATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                                               <  2:652112/133‑1 (MQ=14)
                                                                                 agagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgt                                                             >  2:629513/1‑141 (MQ=255)
                                                                                      gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        <  1:597274/141‑1 (MQ=255)
                                                                                        aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                                      <  1:717523/141‑1 (MQ=255)
                                                                                         aaTACATTCAATACCTTTTAAAATTTTATATTTCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACATTTACAGTCAGgtttaatttt                                                     <  2:754193/141‑1 (MQ=11)
                                                                                          aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTTAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  1:635217/1‑141 (MQ=255)
                                                                                          aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCCGgtttaagttta                                                    <  1:558192/141‑1 (MQ=255)
                                                                                          aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  1:365311/1‑141 (MQ=255)
                                                                                          aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  2:768960/1‑141 (MQ=255)
                                                                                          aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                    >  2:248035/1‑141 (MQ=255)
                                                                                          aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                    >  1:137577/1‑141 (MQ=255)
                                                                                           tACATTCAATACCTTTTAAAGTATACTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                   >  1:575547/1‑141 (MQ=255)
                                                                                             cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                 >  2:735417/4‑141 (MQ=255)
                                                                                                   atatCTTTTAATTTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTATTATAATATGACGGTGATCTTGCTCTATGAGGTTAGTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTa                                           <  1:96378/141‑1 (MQ=11)
                                                                                                    ttcctttttatggtttCTTTGCCGTATTGCTACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGCGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTaa                                          <  2:225519/127‑1 (MQ=14)
                                                                                                    tACCTGTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTaa                                          <  1:544937/141‑1 (MQ=255)
                                                                                                      ccTTTTAAAATATTATGTGCCGGATTGATGGTGTGGTATTTGGTGTTTCTTGCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCGGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                        >  2:267125/1‑141 (MQ=11)
                                                                                                       cTTTTAAAGTATTCTTGGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                       <  1:255896/141‑1 (MQ=255)
                                                                                                          ttAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTattt                                    <  2:451936/141‑4 (MQ=255)
                                                                                                           tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAGTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTTGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                   >  1:694833/1‑141 (MQ=255)
                                                                                                               gTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCGTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGcc                               <  2:637175/141‑1 (MQ=255)
                                                                                                                tATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCa                              >  2:474748/1‑141 (MQ=255)
                                                                                                                 aTTCTTGTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt                             <  2:199966/141‑1 (MQ=255)
                                                                                                                  ttCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt                            >  1:462750/1‑141 (MQ=255)
                                                                                                                   tCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCCCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTg                           >  1:518181/1‑141 (MQ=255)
                                                                                                                    cTTTGCTCTAGTGATACTTTGATACCTTGTATTTCTTACTTTAATAGGACGGCGATCTTGCCCAATGAGGTGATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc                          <  1:206742/141‑1 (MQ=14)
                                                                                                                       tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCtcc                       >  2:680673/1‑139 (MQ=255)
                                                                                                                         ttGTATTGATACTTTTATATCTTGTCTTTCTTACTTTAATATGACGGTGATCATGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCt                     <  1:375054/140‑1 (MQ=14)
                                                                                                                             aTTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGt                 <  2:238802/141‑1 (MQ=255)
                                                                                                                             aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGt                 >  1:167280/1‑141 (MQ=40)
                                                                                                                              ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGtt                <  1:504920/141‑1 (MQ=255)
                                                                                                                                    cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGt          <  1:762256/141‑1 (MQ=255)
                                                                                                                                     tttGATACCTTGTCTTTCTAACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTg         >  1:473391/1‑141 (MQ=38)
                                                                                                                                      ttGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGc        <  2:407724/141‑1 (MQ=255)
                                                                                                                                      ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        <  2:181067/141‑1 (MQ=255)
                                                                                                                                      ttGAGACCTTGTCTTTCTTACTTTAATATGCCGGTGATCTTGCCAAATGAGGCTCTTCAGATATTTCGAGGTACAATGGCAGTCAGCTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        <  1:45114/141‑1 (MQ=11)
                                                                                                                                       tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGTTATTTCGTTTTACAATGGCAGGCAGGTTTAAGTTTTAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGATGcc       >  1:301800/1‑141 (MQ=11)
                                                                                                                                         aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGACGGTGCCTg     >  1:33560/1‑141 (MQ=255)
                                                                                                                                         aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg     >  2:337717/1‑141 (MQ=255)
                                                                                                                                            ccTTGTCTTTCTTACTTTATTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTACGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTGAGCCATTGCTACCTTCGTTGAAGGTGCCTGATc  >  2:441611/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: