Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,701,061	C→T	8.5%	M266I (ATG→ATA)	USA300HOU_2555 ←	aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,701,061	0	C	T	8.5%	183.7 / 4.9	69	M266I (ATG→ATA)	USA300HOU_2555	aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (30/31);  new base T (3/3);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ATGAACAAATGGCAAATCAGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACATCTTTCCCAT  >  CP000730/2700923‑2701200                                                                                                                                           |                                                                                                                                            aTGAACAAATGGCAAATCAGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAACGCATATATTGCGGCCTCTTGTAATGCACCAAACAt                                                                                                                                           >  1:132919/1‑141 (MQ=255)              aaaTCCGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTtgtg                                                                                                                              <  1:238934/141‑1 (MQ=255)              aaaTCAGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTtgtg                                                                                                                              <  1:705514/141‑1 (MQ=255)               aaTCAGCTTTTGCAAGCATGGCTTCGAATCCGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTtgtgt                                                                                                                             <  1:389228/141‑1 (MQ=255)                     cTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGttt                                                                                                                       >  1:631050/1‑141 (MQ=255)                         tGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTa                                                                                                                   <  2:487678/141‑1 (MQ=255)                         tGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTa                                                                                                                   <  1:675784/141‑1 (MQ=255)                         tGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTa                                                                                                                   <  2:621753/141‑1 (MQ=255)                           cAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACt                                                                                                                 >  2:302294/1‑141 (MQ=255)                              gCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTCCtttt                                                                                                              >  2:753130/1‑141 (MQ=255)                              gCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACtttt                                                                                                              >  2:6438/1‑141 (MQ=255)                                 tGGCTTCGTATCGGTCACGAAGTGTGGTCATTTCATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCa                                                                                                           <  1:178454/141‑1 (MQ=255)                                    cTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAg                                                                                                        >  2:711557/1‑141 (MQ=255)                                          aTCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAGGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCGAAGCTTGaa                                                                                                  >  1:374128/1‑141 (MQ=255)                                              gTCACGACGTGTTTTAGATACGTTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTCCTTTTTCAAAGCTTGAATCat                                                                                              >  1:406987/1‑141 (MQ=255)                                                   gacgTGTTTTAAATACCTTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCtt                                                                                         <  1:147570/141‑1 (MQ=255)                                                            tAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTCTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCaa                                                                                >  1:558986/1‑141 (MQ=255)                                                                tACCTTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGc                                                                            <  1:301113/141‑1 (MQ=255)                                                                 acatttgcttGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCa                                                                           <  2:107349/133‑1 (MQ=255)                                                                       cTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATACTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCg                                                                     <  2:678047/141‑1 (MQ=255)                                                                           gTTCTTCTAAAAAATCATCATAATGATTCAACGCAATTATTGCGGCAGCAAGGAATGCACTTAACATCCCATCATGTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCCTATCTTTATTACCAACTGCAAAACCGActc                                                                 >  1:465089/1‑141 (MQ=255)                                                                           gTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGActc                                                                 >  1:614176/1‑141 (MQ=255)                                                                             tcttctAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACAACAAATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTa                                                               >  1:496989/1‑141 (MQ=255)                                                                              cttctAAAAAATCATCATACGGATGCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGAATCTaa                                                              >  2:364570/1‑141 (MQ=255)                                                                              cttctAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTaa                                                              >  2:773827/1‑141 (MQ=255)                                                                                tgtAAAAAGACAGCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTaaaa                                                            <  2:306952/139‑1 (MQ=255)                                                                                tctAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTCTGCGTTCGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAGCTGCAAAACCGACTCGaaaa                                                            >  1:265079/1‑141 (MQ=255)                                                                                  tAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAAcc                                                          <  2:304563/141‑1 (MQ=255)                                                                                   aaaataTCATCATGATGACTCAAATCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGTATTTGTGTGCGTTTGGTACTTTTTCAAAGCTCGAATCATATCTTTATTACCAACTGCAAAATCGACTCTAAATCCt                                                         <  2:710531/141‑1 (MQ=255)                                                                                   aaaaaaTCATCATAATGATTCAAATCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCTTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCt                                                         <  1:511564/141‑1 (MQ=255)                                                                                   aaaaaaTCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCt                                                         <  2:631050/141‑1 (MQ=255)                                                                                   aaaaaaTCAGCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATGTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCt                                                         <  2:7242/141‑1 (MQ=255)                                                                                    aaaaaTCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTg                                                        <  2:271777/141‑1 (MQ=255)                                                                                     aaaaTCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGa                                                       <  1:374278/141‑1 (MQ=255)                                                                                       aaTCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACa                                                     <  1:692231/141‑1 (MQ=255)                                                                                        atcatcATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACAt                                                    >  2:482308/1‑141 (MQ=255)                                                                                        atagtcacAATGATTCAAGCCATATATTGCGGCATCTTGTAATGCACCAAAAGTCCCAGCATTTGTGTGCGATTGGTAATTGTTCAAAGCTTGAATCATATCTTTATTACCAACTTTAAAACCGCCTCTAAAACCTTACAt                                                    <  2:401952/137‑1 (MQ=255)                                                                                         tcatcaTAATGATTCAAAGCATATATTGCGGCGTCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATg                                                   <  1:103578/141‑1 (MQ=255)                                                                                          catcatAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGt                                                  <  2:20618/141‑1 (MQ=255)                                                                                          catcatAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGt                                                  <  1:138962/141‑1 (MQ=255)                                                                                          cagcatAATTATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACAGCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGt                                                  <  2:103348/138‑1 (MQ=255)                                                                                           atcatAATTCGTCAAAGCATATATTGCGGCATCTTGTAATCCACCACACATCCCAGCATGTGTGTTCGTTTAGTACTTTTTCAAAGCTTTAATCATATCTTTATTACCACCTGTAAAACCCACGCTAAAACCTGACATGtt                                                 <  2:374128/141‑1 (MQ=255)                                                                                            tcatAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTCTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAAGCGACTCTAAAACCTGACATGTta                                                <  2:564160/141‑1 (MQ=255)                                                                                            acatGATGAGTGAAAGCATATATTGCGGCATCTTGTAATTCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATACCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTta                                                <  2:118969/140‑1 (MQ=255)                                                                                             catAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTtat                                               >  1:276645/1‑141 (MQ=255)                                                                                              atAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTtata                                              <  1:624571/141‑1 (MQ=255)                                                                                               tagtGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAACCATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATaa                                             <  2:646547/138‑1 (MQ=255)                                                                                                 aTGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAAcc                                           <  2:49424/141‑1 (MQ=255)                                                                                                     ttCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTa                                       <  2:42429/141‑1 (MQ=255)                                                                                                          aGCATATATTGCGGCATTTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACaa                                  <  2:446103/141‑1 (MQ=255)                                                                                                          aGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTCCGGTCTTACATGTTATAACCTTTAGACaa                                  <  2:760690/141‑1 (MQ=255)                                                                                                          aGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGCCaa                                  >  2:526807/1‑141 (MQ=255)                                                                                                           gCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCTACTCTAAAACCTGACATGTTATAACCTTTAGACAAg                                 >  1:437715/1‑141 (MQ=255)                                                                                                            cATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACaaag                                >  1:624903/1‑139 (MQ=255)                                                                                                            cATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAAATGCAGAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAgg                                >  1:146229/1‑141 (MQ=255)                                                                                                              tatatTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGaa                              >  2:527586/1‑141 (MQ=255)                                                                                                                        cATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACCAGGAATAAATTTCaa                    >  1:86592/1‑141 (MQ=255)                                                                                                                        cATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCaa                    >  2:324456/1‑141 (MQ=255)                                                                                                                         aTCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAAt                   >  1:699367/1‑141 (MQ=255)                                                                                                                          tCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATc                  >  2:41262/1‑141 (MQ=255)                                                                                                                           cTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCg                 >  2:474169/1‑141 (MQ=255)                                                                                                                           cTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCg                 >  1:737696/1‑141 (MQ=255)                                                                                                                           cTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCg                 >  1:266964/1‑141 (MQ=255)                                                                                                                             tGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCCTTAGACAAGGAATAAATTTCAATCGCa               >  2:595822/1‑141 (MQ=255)                                                                                                                                 aTGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACAt           <  1:136997/141‑1 (MQ=255)                                                                                                                                    cACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTGGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACATCtt        >  1:347703/1‑141 (MQ=255)                                                                                                                                       cAAATATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATTTTATAACCTTTATACAAGGAATAAATTTCAATCGCAACATCTTTcc     >  1:352968/1‑141 (MQ=255)                                                                                                                                       cAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACAGCTTTcc     >  2:191415/1‑141 (MQ=255)                                                                                                                                          aCATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACATCTTTCCCAt  >  2:725960/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                            ATGAACAAATGGCAAATCAGCTTTTGCAAGCATGGCTTCGAATCGGTCACGACGTGTTTTAAATACATTGCTTTGTTCTTCTAAAAAATCATCATAATGATTCAAAGCATATATTGCGGCATCTTGTAATGCACCAAACATCCCAGCATTTGTGTGCGTTTGGTACTTTTTCAAAGCTTGAATCATATCTTTATTACCAACTGCAAAACCGACTCTAAAACCTGACATGTTATAACCTTTAGACAAGGAATAAATTTCAATCGCAACATCTTTCCCAT  >  CP000730/2700923‑2701200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑