BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,161	T→C	29.4%	I131V (ATT→GTT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,161	0	T	C	29.4%	789.4 / inf	790	I131V (ATT→GTT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (236/315); new base C (98/133); total (337/453)
Fisher's exact test for biased strand distribution p-value = 9.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 810 total aligned reads displayed.

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

ctttcttACTTTCATATGTCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAAt                                                                                                                                              >  2:480160/1‑141 (MQ=255)

ctttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAAt                                                                                                                                              >  1:14460/1‑141 (MQ=255)

 tttcttACTTTAATATGACGGTGATGTTGCTCAATGAGGTTATTCACATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGACTGATCTGTAAtt                                                                                                                                             >  2:397577/1‑141 (MQ=11)

 tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCACAGAAGATTTCGTTT‑TAGGTGCCTGATCTGTAAtt                                                                                                                                             <  1:781471/141‑1 (MQ=11)

    tttACCTTACTATGACGGTGATCTTCCTCAATGGGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTAcc                                                                                                                                          <  2:1271/140‑1 (MQ=11)

    cttACTTTAATATGACGGTGATCTTGCTCAATGGGGTTATTCCGATACTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTCTTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTAcc                                                                                                                                          <  2:505318/141‑1 (MQ=11)

        cTTTAATATGACGGTGATCTTTCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCtttt                                                                                                                                      <  1:274856/141‑1 (MQ=255)

         tttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTCTCCATTGCTACCTTCGTTG‑CAGGTGCCTGATCTGTAATTCCCTTTTg                                                                                                                                     >  1:155393/1‑141 (MQ=11)

          ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACGTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGa                                                                                                                                    <  2:243000/141‑1 (MQ=255)

           tAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAg                                                                                                                                   >  2:373459/1‑141 (MQ=255)

            aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                                                                                                  >  2:351234/1‑141 (MQ=255)

               atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACATTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGttt                                                                                                                               <  1:540518/141‑1 (MQ=255)

                tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGAAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAGTAGCGCTACCTTAGTTC‑GAGGAGCCTGCTCTGTGATTACCTGTTGAGGtttt                                                                                                                              >  1:694458/1‑140 (MQ=11)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATTTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                             <  1:338595/141‑1 (MQ=11)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGtttcc                                                                                                                             >  2:467724/1‑139 (MQ=255)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                             <  1:762897/141‑1 (MQ=11)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCAATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                             <  1:638494/141‑1 (MQ=11)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAcc                                                                                                                            >  1:192527/1‑141 (MQ=255)

                     gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTGCGTTG‑AAGGTGCCTGATCTTTAATTACCTTTTGAGGTTTACCaaa                                                                                                                         <  2:275270/141‑1 (MQ=255)

                       gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGGTCTGTAGTTACCTTTTGAGGTTTACCaaaat                                                                                                                       >  2:412905/1‑139 (MQ=11)

                       gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                       >  2:255444/1‑141 (MQ=12)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTGGTTG‑ACGGGGCCTGGTCTGTAATTACCTTTTGAGGTTTACTAAATTGt                                                                                                                     >  2:83179/1‑141 (MQ=11)

                          cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                    >  1:46193/1‑141 (MQ=17)

                           ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGttt                                                                                                                   >  1:2034/1‑141 (MQ=12)

                             gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                 <  2:767765/141‑1 (MQ=17)

                             gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                                                                                                 <  2:523188/141‑1 (MQ=11)

                             gCTCAATGAGGATATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                                                                                                 >  2:14403/1‑141 (MQ=11)

                                  aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATtaga                                                                                                            >  1:100012/1‑141 (MQ=12)

                                  aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgaga                                                                                                            <  1:303435/141‑1 (MQ=17)

                                     gggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  2:492283/140‑1 (MQ=12)

                                     aGGTTATTCAGATATTTCGATGTACAGTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  1:415452/141‑1 (MQ=12)

                                     aGGTTAGTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCGTTAAATATTTTAGCCATTGCTGTCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                         <  2:282155/141‑1 (MQ=11)

                                      ggTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAACCACTCTAACTATTTTGGCCATTGATGCCCCCACCGCACGGTG‑CTTAGCGTGAACGACCTTGTGAGGCTTACCAAATTGTTTAATGAGACggt                                                                                                        >  2:166028/1‑139 (MQ=2)

                                         tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTACTTACTTTAGCCATTGCTACCTTCGTGG‑AAGGAGCCGGAGCTGGAATTACCTTGTGAGGTTTACCAACTTGTTTAATGCGACGTTTgt                                                                                                     >  1:227244/1‑140 (MQ=11)

                                          aTTCAGATATTTTGATGTATGAGGTCTGTTAGGTTTAAGTTTAACAGCTTTAATGACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                    <  2:652209/141‑1 (MQ=11)

                                          aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTAAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                    >  1:112391/1‑141 (MQ=12)

                                           ttCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATa                                                                                                   <  1:622126/141‑1 (MQ=17)

                                            tCATATATTTCGATGTACTATGACAGTCAGGTTTAAGTTAAAAATCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATaa                                                                                                  <  1:534577/141‑1 (MQ=11)

                                            tCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                                                                                                  <  2:106158/141‑1 (MQ=17)

                                             cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCGTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                 <  1:591766/141‑1 (MQ=12)

                                             aaGATATTTACATGTATAATGGCAGTTAGGTTTAAGTTTAAAAGCTTTACTTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                 <  1:552271/140‑1 (MQ=11)

                                               gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                               <  1:441276/141‑1 (MQ=17)

                                                atatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGGGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                                                                                              <  2:606840/141‑1 (MQ=11)

                                                 tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                             <  1:239005/141‑1 (MQ=12)

                                                 tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGCGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                             <  1:360728/141‑1 (MQ=11)

                                                    ttCGATGTTCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                          <  1:566604/141‑1 (MQ=12)

                                                    ttCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTATTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                          >  1:736462/1‑141 (MQ=17)

                                                      cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑GAGGTGCCTGATCTATAATTACCTTTTGAGGTTTACCGAATTGTTTAATGAGACGTTTGATAAACGCatatac                                                                                        >  2:590376/1‑139 (MQ=11)

                                                      cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                        <  1:28796/141‑1 (MQ=12)

                                                       gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATTAAGCCATATGCt                                                                                       >  2:638641/1‑141 (MQ=11)

                                                       gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGcg                                                                                       >  2:703076/1‑140 (MQ=12)

                                                       gATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                       >  1:748877/1‑141 (MQ=12)

                                                        aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGTTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATAGGACGTTTTTTAAACGCAGTTGCtt                                                                                      >  1:5077/1‑140 (MQ=11)

                                                         gggaCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa                                                                                     <  2:220462/138‑1 (MQ=11)

                                                            aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  >  1:318568/1‑141 (MQ=12)

                                                            aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  <  1:16833/141‑1 (MQ=255)

                                                             ccaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 <  2:738601/139‑1 (MQ=11)

                                                             cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACGTATTTGTTTAATGAGTCGTTTGTTAATCGCTTATGCTGGATgt                                                                                 >  1:255869/1‑140 (MQ=11)

                                                              aaTGGCAGTTAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                <  1:51742/141‑1 (MQ=9)

                                                               aTGACATTCAGGTTTAAGTTTAAAAGCTTTATTTACTTTAGCCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAACTAACTTTAGCTAATTACGGTATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                                               <  2:678735/141‑1 (MQ=11)

                                                               aTGACAGTCAGGTTTAAGTTTAACAGACTTCATCACCTAAGCCAATGCTACCTTCGTTG‑AAGGTGCACGATTTGTAATGATCTCTCGTTAATTACCAAAGTGTTTAATGAGACGTTCGATAAACGCATATGGTGAATGAtt                                                                               <  1:577177/141‑1 (MQ=2)

                                                               aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGACTGAtt                                                                               >  2:183282/1‑141 (MQ=11)

                                                                tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa                                                                              >  2:710621/1‑141 (MQ=11)

                                                                tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa                                                                              <  2:573580/141‑1 (MQ=11)

                                                                                                   tttAACCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  1:351234/141‑1 (MQ=11)

                                                                                                         cATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  2:358925/141‑1 (MQ=32)

                                                                                                          aTTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                    >  1:123005/1‑141 (MQ=17)

                                                                                                          aTTGCGGCCTTCGTTG‑AAGGTGTCTGATCTGTAACCACCTTTTGAGGCTTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                    <  1:403710/141‑1 (MQ=11)

                                                                                                           ttGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCATCCAAATATCTAATGTATGTCCCTCTg                                   >  1:476775/1‑141 (MQ=17)

                                                                                                            tGCTACCTCCGTTG‑AAGTTGTCTGATCTGGAATTACCTTTTGAGGTTTACCAAATTGGTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgt                                  <  1:684948/141‑2 (MQ=11)

                                                                                                             gCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACTTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTgta                                 <  1:431493/141‑3 (MQ=12)

                                                                                                              cTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTCATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgtat                                <  1:86238/141‑4 (MQ=17)

                                                                                                               tACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                               >  2:305065/1‑141 (MQ=17)

                                                                                                               caCCTTTGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                               <  2:430082/140‑1 (MQ=17)

                                                                                                                aCCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTATGCAACCAAATATCTAATGTATGTCGCTCTGCATCa                              >  1:653432/1‑141 (MQ=17)

                                                                                                                aCCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCCTACGCAACCAAATATCTAATGTATGTACCTCTGCATCa                              >  2:447623/1‑141 (MQ=17)

                                                                                                                  cgtCGTTG‑AGGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGGCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCTTCTGTATCAAt                            <  2:380828/139‑1 (MQ=11)

                                                                                                                  cTTCGTTT‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCATATATCTAATGTATGTCCTTCTGTATCAAt                            <  2:579459/141‑1 (MQ=11)

                                                                                                                  cTTCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                            <  2:755677/141‑1 (MQ=255)

                                                                                                                   ttCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCGCTGCATCAATg                           >  2:11348/1‑141 (MQ=17)

                                                                                                                   ttCGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                           >  2:32986/1‑141 (MQ=12)

                                                                                                                     cGTTG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGc                         >  1:265942/1‑141 (MQ=12)

                                                                                                                      tttG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                        <  2:315797/140‑1 (MQ=255)

                                                                                                                       ttG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                       <  1:629513/141‑1 (MQ=17)

                                                                                                                        tttaaGGTGCCTGATCTTTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                       <  1:666680/138‑1 (MQ=11)

                                                                                                                        tG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                      >  1:677722/1‑141 (MQ=32)

                                                                                                                        tG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                      >  2:5110/1‑141 (MQ=32)

                                                                                                                        tG‑AAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTTCCTCTGTATCAATGGCACg                      >  1:49238/1‑141 (MQ=255)

                                                                                                                            aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGgta                   >  1:133022/1‑141 (MQ=17)

                                                                                                                            aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGatt                   >  2:665022/1‑140 (MQ=17)

                                                                                                                             ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                  <  2:2034/141‑1 (MQ=17)

                                                                                                                              gTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                 >  2:624018/1‑141 (MQ=12)

                                                                                                                                 ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATACAt              >  1:384586/1‑141 (MQ=17)

                                                                                                                                 ccTGATCTGATATTAGCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGAGACGATATAAAt              >  2:72304/1‑141 (MQ=11)

                                                                                                                                     aTCTGCAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt          <  1:6108/141‑1 (MQ=17)

                                                                                                                                       cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAAATAAATAGCTcc        >  2:152164/1‑141 (MQ=17)

                                                                                                                                        tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa       <  2:780176/141‑1 (MQ=17)

                                                                                                                                         gTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt      <  2:260610/141‑1 (MQ=255)

                                                                                                                                          tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAGCAATGGCACGATATAAATAGCTCCAtt     <  2:548129/141‑1 (MQ=17)

                                                                                                                                             ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc  <  1:723006/141‑1 (MQ=32)

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTG‑AAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`