BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	55.7%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	55.7%	163.4 / inf	991	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (213/213); new base A (252/291); total (480/511)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 995 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTT  >  CP000730/36175‑36454

 tACCAATTTGTTTAATGAGACGTTCGATAAACGCCTATGCTGCATGATTATCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGt                                                                                                                                            <  1:535106/141‑1 (MQ=11)

  tccAAATCGTCTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCGCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                                                                           <  2:711175/140‑1 (MQ=255)

  aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGGCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtata                                                                                                                                           >  2:203766/1‑141 (MQ=12)

      aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAACTAATGTATGTCCCTCTACATCACTGGCACGATATAAAAAGCTCCAATTCACTTTTATTTTGATGGACGtc                                                                                                                                       >  2:693368/1‑141 (MQ=11)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATTTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGtct                                                                                                                                      >  1:167639/1‑141 (MQ=17)

        ttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtctc                                                                                                                                     >  1:225225/1‑141 (MQ=17)

         tGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtca                                                                                                                                    <  1:731111/141‑1 (MQ=12)

          gTTTAATGAGACTTTTGATAAACTCATATGCTGAATGATTATCTCGTTGCTTACGCAATCAAATATCTAATGTATGTCCCTCTGTATCAATGACACGATATAAATAGCTCCCTTTTTCTTTTATTTTGATATACGTCtcat                                                                                                                                   <  2:48639/141‑1 (MQ=11)

            ttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcatca                                                                                                                                 >  1:528108/1‑141 (MQ=32)

              taTGAGACGTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                               <  1:29689/140‑1 (MQ=17)

                  agaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGc                                                                                                                           <  1:216137/141‑1 (MQ=12)

                   gaCGTTTGATAAACGCAGATGCTGAATGATTATCCCGTTTCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                                                                          <  2:373229/141‑1 (MQ=11)

                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATCAATACGCCa                                                                                                                         >  1:308904/1‑141 (MQ=12)

                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTAGGTTTGATCAAtgatcga                                                                                                                         >  1:763559/1‑135 (MQ=11)

                          gATAAACGCATATACTGAATGATTATCTCGTTGAAATTAACACCAAATAAATTGTTTTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATGAATGCGCCATTTGta                                                                                                                   >  1:379848/1‑141 (MQ=2)

                            tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaat                                                                                                                 >  2:648965/1‑141 (MQ=17)

                            tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaat                                                                                                                 <  1:34620/141‑1 (MQ=32)

                               aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAg                                                                                                              >  2:463009/1‑141 (MQ=34)

                                    tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATCAGCCATTTGTAATAAGCtttt                                                                                                         <  2:723339/141‑1 (MQ=255)

                                     atGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCttttt                                                                                                        >  1:677558/1‑141 (MQ=255)

                                      tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTTTGTATCAATGGCACGATATAAATAGCTCCATTTTTCTTTGATTTGTTTATATGTCTCATCATAACTCTATTTTTAATAAATtttttt                                                                                                       >  1:450630/1‑141 (MQ=11)

                                      tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCGAATGTATGTCCCTCTGTATCAATGGCACGATATAAAGAGCTCCATTTTCCTTTTATTTTGATATACGGCTCATCAATACGACATTTGTAATATGAttattt                                                                                                       >  2:740287/1‑141 (MQ=11)

                                        cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                     >  2:447640/1‑141 (MQ=38)

                                          gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                                                                   <  1:584318/141‑1 (MQ=39)

                                           aagtaTTATCTCCTTGCGTACCGAACCAAATCTCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCAATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                                                                  <  2:6176/137‑1 (MQ=14)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTATACGTCTCATCAATACGCGATTTGTAATCTGCTTTTTTAAGtttttt                                                                                              >  1:656987/1‑141 (MQ=11)

                                                ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                                                             <  1:628021/141‑1 (MQ=255)

                                                ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                                                             >  2:670016/1‑141 (MQ=40)

                                                 tATCTCGTTGTTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                            <  2:337958/141‑1 (MQ=255)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCATGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                            >  2:83644/1‑141 (MQ=255)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                            <  1:644503/141‑1 (MQ=255)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATAAGTCTCATCTAGACGCCCGTAGTAAGAAGTTTTTGTAATCtttttat                                                                                            >  1:593474/1‑139 (MQ=11)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACCATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCCTTTTTATGCTTTttct                                                                                            <  1:470261/141‑1 (MQ=255)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCGTCTGGATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCtttttttt                                                                                           >  1:743581/1‑141 (MQ=14)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATGTGTAATAAGCTTTTTTATGCTTTttctt                                                                                           >  1:87446/1‑141 (MQ=255)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                           <  1:505383/141‑1 (MQ=40)

                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCCATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                          <  2:395368/141‑1 (MQ=255)

                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                          <  2:435701/141‑1 (MQ=255)

                                                   tctcGTTGCTGACGCAACCAAATATCTAATGTAGGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTGTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                          <  1:103944/141‑1 (MQ=255)

                                                    ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCCCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTATGTCTCATCAATACGCCCTTTGTAATAAGCTTTTTTATTCTTTTTCTTcc                                                                                         >  2:28769/1‑141 (MQ=255)

                                                     tcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCa                                                                                        >  2:501444/1‑141 (MQ=40)

                                                       gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaat                                                                                      >  2:572186/1‑140 (MQ=40)

                                                           cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTg                                                                                  >  1:431913/1‑141 (MQ=255)

                                                           cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCAAATTTg                                                                                  >  2:237906/1‑141 (MQ=255)

                                                           cTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATGAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCAATTTGTAATAAGCTTTTTTATGCTTTTTTTTTCAAATTTg                                                                                  >  2:129676/1‑141 (MQ=255)

                                                            ttACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTgt                                                                                 >  2:60161/1‑140 (MQ=255)

                                                             tACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAt                                                                                <  1:422772/141‑1 (MQ=255)

                                                               cGCAACCAAATATCTAATGTATGTTCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                              <  1:14403/141‑2 (MQ=255)

                                                               agcacccacatcacTAACGTATGGCCATCTGCATTATTGGCACGTTAGAAATAGCTCCATGTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAc                                                                              <  2:541957/128‑1 (MQ=14)

                                                               agCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                              <  1:326028/140‑2 (MQ=255)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                            >  2:264713/1‑141 (MQ=38)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGCATCAAGGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACaa                                                                            <  2:227244/141‑1 (MQ=255)

                                                                  ccataactatCTAGTGTTTGTCCCTCTGCATCAATGGCACGATATAAAGAGCTCAATCTTCCTTTTATTTTGATGGACGTCTCATCAATACGCCATTTGTAACAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                           <  2:77939/134‑1 (MQ=11)

                                                                    ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATCCTTTTTCTTTCAAATTAGATTTAAAAt                                                                         >  1:489928/1‑141 (MQ=11)

                                                                     cAAATATCTAATGTATGTCCCACTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCACTACGCCATTTGTAATAAGCTTTTTTATGCTCTTTCTTCCAAATTTGATATAAAAtt                                                                        >  2:175633/1‑141 (MQ=11)

                                                                       aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                      <  1:461472/141‑1 (MQ=25)

                                                                          atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggac                                                                   >  1:559918/1‑139 (MQ=255)

                                                                          atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                   <  1:356641/141‑1 (MQ=255)

                                                                           tCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACTTCTCATCAATACGCCATTTGTGATAAGCTTTTTTATGCTTTTTCTTCCGAATTTGATACGAAATTTGGGCa                                                                  >  1:157457/1‑141 (MQ=14)

                                                                             tAATTTATGTCCCTCGGTATCAATGGCACGATATAAATAGCTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                <  2:301800/141‑1 (MQ=11)

                                                                             tAATGCATGTCCCTCTGTATTAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                <  1:390602/141‑1 (MQ=11)

                                                                              aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                               >  1:192375/1‑141 (MQ=255)

                                                                              aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                               <  1:710082/141‑1 (MQ=255)

                                                                                 gtctgtCCCGCGGTAGCAAGGGCACGATATAAATATCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                            <  1:80827/138‑1 (MQ=11)

                                                                                      gcccTCTGCATCAATGGCACGATCTAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                       <  1:693368/140‑1 (MQ=16)

                                                                                       cccTCTGTTTCCATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc                                                      <  2:147705/141‑1 (MQ=11)

                                                                                       cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc                                                      <  2:156913/141‑1 (MQ=255)

                                                                                              taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATCTAAAATTGGGGCATATTCTTGGACCCATCGGt                                               >  1:118178/2‑141 (MQ=11)

                                                                                              taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgtt                                               >  2:244869/2‑139 (MQ=255)

                                                                                              cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                               <  2:348746/141‑1 (MQ=18)

                                                                                               aTCAATGGCACGATATGAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                              <  2:380941/141‑1 (MQ=21)

                                                                                                  aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGTTTAACCCAACGGTAGat                                           >  1:274314/1‑140 (MQ=255)

                                                                                                   atcgCACGATACAACAAGCTCCATCTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                          <  2:403943/138‑1 (MQ=11)

                                                                                                        gcGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTATATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                     <  2:193841/140‑1 (MQ=255)

                                                                                                        aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTCCGTCTCATCAATACGCCATTTGTAATACGCTTTTTTCTGCTTTTTCTTCCAAATATGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                     <  2:514355/141‑1 (MQ=11)

                                                                                                        aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                     <  1:367592/141‑1 (MQ=255)

                                                                                                         cGATATAAATAGCTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTGTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                    >  1:407385/1‑141 (MQ=11)

                                                                                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                    >  1:719881/1‑141 (MQ=255)

                                                                                                             ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTATTTTTCTTACAAGTTATATACAAAGTTGGGGGATATTCTGGAACCGACAGGGAGACAGTTGActgat                                >  2:590651/1‑141 (MQ=14)

                                                                                                             ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTCTTGTACCCAACGGTAGATCGTTACatgag                                >  2:119462/1‑140 (MQ=11)

                                                                                                             aaaaaTAGCCCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                <  2:279769/139‑1 (MQ=11)

                                                                                                                 atacctcctctgtCCTTTTTTTTTTATTCACGTCTCATCAATACGCCATTTGTTATAAGCTTTTTTATGCTTTTTCTTCTATATTTTATATAATATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                            <  1:180131/129‑1 (MQ=11)

                                                                                                                 aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                            <  1:240770/141‑1 (MQ=18)

                                                                                                                   aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                          >  2:103248/1‑141 (MQ=255)

                                                                                                                          ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                   <  1:618454/141‑1 (MQ=18)

                                                                                                                          ttttCCTTTGATTTTGATATACGTCTCAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                   <  1:600950/141‑1 (MQ=11)

                                                                                                                            ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGtggtgtaaac                 <  2:631977/141‑10 (MQ=255)

                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTATTGAACCAAAAGGTCGATGGTTGAATGATGAAAGTTTAaaccac                 >  2:416965/1‑141 (MQ=11)

                                                                                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                >  1:776431/1‑141 (MQ=255)

                                                                                                                              ccTTTTCTCTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt               <  1:47865/141‑1 (MQ=11)

                                                                                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTTATTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACAGTTGAATGATGAACGTTTACACCACGt               >  1:542224/1‑141 (MQ=11)

                                                                                                                               cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt              >  1:127458/1‑141 (MQ=25)

                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCTAACGGTAGATCGTTGGATGATGAACGTTTAtacctcttt              >  2:530289/1‑136 (MQ=11)

                                                                                                                                  ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc           >  1:284542/1‑141 (MQ=18)

                                                                                                                                    aTTTTTATATCCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTAGGGTATATTGTTGAACCCAACGGCAGTTAGTGCAATGATGAACGTTTACTGCACCTTCTCtt         <  2:757451/141‑1 (MQ=2)

                                                                                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt         <  1:575568/141‑1 (MQ=11)

                                                                                                                                      tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa       <  1:665022/141‑1 (MQ=21)

                                                                                                                                      tttCATATACGTCTCATCAATACGCCATTTGTAATAAAGTTTTTTATGCTTTTTTTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa       <  2:553593/141‑1 (MQ=11)

                                                                                                                                       ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat      <  2:308904/141‑1 (MQ=255)

                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat    >  1:267132/1‑141 (MQ=25)

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTT  >  CP000730/36175‑36454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`