Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,581 C→T 37.5% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5810CT37.5% 156.8 / 137.0 139intergenic (‑30/‑493)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/43);  new base T (17/35);  total (61/78)
Fisher's exact test for biased strand distribution p-value = 5.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-02

Only 100 of 139 total aligned reads displayed.

TCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAG  >  CP000730/36442‑36720
                                                                                                                                           |                                                                                                                                           
tCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACt                                                                                                                                            >  2:497075/1‑141 (MQ=32)
tCCCTTAACATATCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACt                                                                                                                                            <  2:507431/141‑1 (MQ=17)
      aaTATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAt                                                                                                                                      <  2:157457/141‑1 (MQ=35)
            tCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACt                                                                                                                                >  1:440501/1‑141 (MQ=35)
             cAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACtt                                                                                                                               >  2:773679/1‑141 (MQ=35)
                 tatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAATTTATAATATAAATTTAACTTTGCa                                                                                                                           >  2:471689/1‑141 (MQ=255)
                 tatatCACGATAACTCAACGGATATCGTAGATAGAAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATGCAGAAGACTCCTTTTTGTTAAAATTATACTCTAAATTCAACTTTGCa                                                                                                                           <  1:202110/141‑1 (MQ=11)
                 taaatCACGATAACTAAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa                                                                                                                           <  1:353346/138‑1 (MQ=21)
                   tatCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAAAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAc                                                                                                                         <  1:416965/141‑1 (MQ=255)
                        cGATAACTCAATGTATATCTTAGATAGTAGCCATCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAc                                                                                                                    >  2:520093/1‑141 (MQ=25)
                         gATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAccc                                                                                                                   >  2:193791/1‑141 (MQ=25)
                           tagcTCAGTGCATATCTGATATAGGAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGGTTGGATCTTAAATAGTTCAGACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  1:550511/138‑1 (MQ=11)
                           tagcTCAATGCACATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACCCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAccacacctt                                                                                                                 <  2:683328/138‑9 (MQ=11)
                            agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGta                                                                                                                >  1:640460/3‑141 (MQ=9)
                            agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGta                                                                                                                >  1:756098/3‑141 (MQ=12)
                             gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               >  2:788733/2‑141 (MQ=12)
                             gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               >  2:231193/2‑141 (MQ=12)
                             gcTCAATGCATATCTTAGATAGTAGCAAACGGCTACAGTGATAACATCCTGGTTAAATTGTTTATATCTGAAATAGATCATACACAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACTGAACCGtat                                                                                                               >  2:190875/2‑141 (MQ=11)
                              cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                              >  1:171321/1‑141 (MQ=16)
                              cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                              <  2:702947/141‑1 (MQ=18)
                              cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                              >  1:710573/1‑141 (MQ=12)
                               ccAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                             <  1:66445/140‑1 (MQ=40)
                                cAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATAATTGCTGGACGTTGGATGTCTGAATCCTTGCATACAGAAGACTCCTTTTGGTTAGAAGGATACTATGAATTCAACTTTGCAACAGAACCGtattat                                                                                                            >  1:777847/1‑141 (MQ=9)
                                cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                                                                                                            <  1:129214/141‑3 (MQ=255)
                                cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                                                                                                            <  1:60161/141‑3 (MQ=255)
                                cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                                                                                                            <  2:101883/141‑3 (MQ=255)
                                 aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttta                                                                                                           >  2:583819/1‑138 (MQ=255)
                                   tgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  2:489681/138‑6 (MQ=255)
                                   tgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  1:41217/138‑6 (MQ=255)
                                   tgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  2:717220/138‑6 (MQ=255)
                                   tgcatatCTTAGATAGTAGCCAACGGATACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGTCTCCTATTTGTTAAAATTATATTATAAATTCAACTTTCCAACAGAACCGtattttatg                                                                                                         <  1:481284/138‑6 (MQ=11)
                                   tgcatatCTTAGACAGTAGCTAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  2:316500/138‑6 (MQ=11)
                                   tgcaaatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTGAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  1:462470/136‑6 (MQ=11)
                                   tgcaaatCTTAGATAGTAGACAACGGCTACAGTGATAACATAATTGATGTATCATTGATATTTGATATAGTTCATAAAGCAGCCTCCTGGGTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  2:756887/136‑6 (MQ=2)
                                   tgaatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGATTACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  2:22029/138‑6 (MQ=11)
                                   agcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAACTTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  2:437433/138‑6 (MQ=255)
                                    tgatatCTTAGAAGATAGCCAACGGCTACAGTGATAACATCCTTGTTAAATGGTTTATATCGGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                        <  2:118178/139‑7 (MQ=11)
                                    gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                        <  1:623707/139‑7 (MQ=255)
                                    gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                        <  2:208718/139‑7 (MQ=255)
                                    gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                        <  1:469052/139‑7 (MQ=255)
                                    gcatatCGTAAATTGTAGCCAACGGGTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCACCAGAACCGtattttatga                                                                                                        <  2:654722/139‑7 (MQ=11)
                                    gTACATCTTAGATAGTAGCCAACCGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATACTTCATACAGAAGACTCCTTCTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgta                                                                                                        <  2:410062/141‑12 (MQ=11)
                                     catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCTTGCTTGGTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                                                                                                       >  1:739085/2‑134 (MQ=11)
                                     catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCCACCGACCCGtattttatgag                                                                                                       <  2:597016/140‑8 (MQ=11)
                                     catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                                                                                                       >  1:667737/2‑134 (MQ=255)
                                       tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtctta                                                                                                     >  1:702189/1‑127 (MQ=11)
                                       tatCTTAGATAGTAGCCAACGACTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATCAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                     >  1:409114/1‑132 (MQ=11)
                                       tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                     <  2:183673/141‑15 (MQ=11)
                                       tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                     >  1:424738/1‑127 (MQ=11)
                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATaga                                                                                                    >  2:24710/1‑141 (MQ=255)
                                         tCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaca                                                                                                   >  2:596765/1‑130 (MQ=11)
                                          cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCAtattttatgagtacac                                                                                                  >  1:144475/1‑129 (MQ=11)
                                           tttgATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGAt                                                                                                 <  1:268896/138‑1 (MQ=255)
                                            tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                                <  2:655850/141‑15 (MQ=11)
                                               aTAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTg                                                                                             <  2:775024/141‑1 (MQ=255)
                                               aTAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTAATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCACCAGAACCTTATTATGGAATAGAGATGTTg                                                                                             >  2:196791/1‑141 (MQ=255)
                                                tagtagGCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGGACCGTATTATGGAATAGAGATGTTgg                                                                                            >  1:578934/1‑141 (MQ=255)
                                                  gtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTa                                                                                          >  1:659397/1‑141 (MQ=255)
                                                  gtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACACAACCGTATTATGGAATAGAGATGTTGGTa                                                                                          >  2:552325/1‑141 (MQ=255)
                                                    agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAAc                                                                                        <  2:784842/141‑1 (MQ=255)
                                                      ccAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAGAGAAGGCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAAcct                                                                                      >  1:609582/1‑139 (MQ=255)
                                                      ccAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAt                                                                                      >  1:315285/1‑141 (MQ=255)
                                                       cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtt                                                                                     <  1:332236/141‑1 (MQ=255)
                                                          cGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCATCAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtat                                                                                  >  2:553650/1‑141 (MQ=255)
                                                          aggCTACAGTGATAACATCCTTCTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTGTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtat                                                                                  <  2:57802/140‑1 (MQ=255)
                                                              tACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAg                                                                              <  1:18857/141‑1 (MQ=255)
                                                              tACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAg                                                                              >  1:500749/1‑141 (MQ=255)
                                                                cAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATGGAGATGTTGGTAACAtttgttcatga                                                                            >  2:695896/1‑133 (MQ=17)
                                                                cAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGa                                                                            >  1:352817/1‑141 (MQ=255)
                                                                         cATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATAc                                                                   <  2:320265/141‑1 (MQ=255)
                                                                          aTCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTTTACt                                                                  >  2:788609/1‑141 (MQ=21)
                                                                               tGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTCAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGt                                                             <  1:304458/141‑1 (MQ=255)
                                                                               tGTTAAATGGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGt                                                             <  1:304372/141‑1 (MQ=255)
                                                                                tttAAATTGTTTATATCTTAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTTAGtt                                                            <  2:289400/140‑1 (MQ=255)
                                                                                gTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTATACTATAAATTCAACTTTCCAACAGAACAGTATTATGGAGTAGAGATGTTTGTAACATTTATCCAGTATCATTATACTTAAAtt                                                            >  2:389562/1‑141 (MQ=18)
                                                                                 ttAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACACTTATACAGGATCATTATACTTAAGttt                                                           <  2:599114/141‑1 (MQ=255)
                                                                                   aaaTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTaa                                                         >  1:630685/1‑141 (MQ=255)
                                                                                   aaaTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTaa                                                         >  1:19389/1‑141 (MQ=255)
                                                                                    aaTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAt                                                        <  1:144508/141‑1 (MQ=255)
                                                                                    aaTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAt                                                        <  1:638343/141‑1 (MQ=255)
                                                                                                   aaaTCGGTCATACAGAAGGCTCCTTTTGGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGaa                                         <  1:92202/141‑1 (MQ=255)
                                                                                                        gTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAAAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcaca                                    <  1:773679/141‑1 (MQ=255)
                                                                                                          tCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacaca                                  >  2:339417/1‑141 (MQ=255)
                                                                                                          tCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTAAACTTTGAAACAGAACCGACTTATGGAAAAAAGATGTTGGTAAAATTTCTAAAGGATCATCATCCTTAAGTTTAATTTCGTTATTAAAGAAcaaaaaa                                  >  1:355074/1‑135 (MQ=21)
                                                                                                             tACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACATATTc                               <  1:535678/141‑1 (MQ=255)
                                                                                                              aCAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTcc                              >  1:588201/1‑141 (MQ=255)
                                                                                                                 gaagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAAc                           >  1:423538/1‑141 (MQ=255)
                                                                                                                  aagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTCAAGTTTAATTTCGTTATTACAGCACCACACATTCCAAcc                          <  1:394832/141‑1 (MQ=255)
                                                                                                                    gaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCag                        <  2:451434/141‑1 (MQ=255)
                                                                                                                      cTCCTTTTTTTTTTTATTTTACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTATTTTCGTTATTACAGAACCACACATTACAACCagaa                      >  2:471089/1‑141 (MQ=255)
                                                                                                                       tCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCagaag                     >  1:377857/1‑141 (MQ=255)
                                                                                                                        ccTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGATATGTTGGTAACATTTATACAGGATCATTATACTTACGTTTAATTTCGTTATGACAGAACCACACATTCCAACCagaagg                    >  2:286402/1‑140 (MQ=255)
                                                                                                                          tttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAgaga                  <  2:512139/141‑1 (MQ=255)
                                                                                                                          tttttGTGAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAgaga                  <  2:467483/141‑1 (MQ=255)
                                                                                                                           ttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGaa                 >  1:677100/1‑141 (MQ=255)
                                                                                                                                 tAAAATTATACTATAAATTCAACTTTGAAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATg           <  1:497075/141‑1 (MQ=255)
                                                                                                                                   aaaTTATACTATAAATTCAACTTGGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTGCAACCAGAAGAGAAAGTATGTc         <  2:576575/141‑1 (MQ=255)
                                                                                                                                    aaTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCt        <  1:581495/141‑1 (MQ=255)
                                                                                                                                        ataCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTAttt    >  1:626547/1‑141 (MQ=255)
                                                                                                                                          aCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAg  <  1:337639/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTAG  >  CP000730/36442‑36720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: