Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,850 C→T 39.5% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8500CT39.5% 131.7 / 91.4 108intergenic (+81/+27)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (35/30);  new base T (12/31);  total (47/61)
Fisher's exact test for biased strand distribution p-value = 9.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

CATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTT  >  CP000730/35712‑35981
                                                                                                                                           |                                                                                                                                   
cATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCa                                                                                                                                   >  2:710398/1‑141 (MQ=255)
 aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                                                  >  1:328275/1‑141 (MQ=255)
 aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                                                  >  1:322350/1‑141 (MQ=255)
  ttGACAAAGTATTTAATGTAGAAGCTAGTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACt                                                                                                                                 <  2:306001/141‑1 (MQ=255)
       aaaGTAATTAAGCTTGAAGCTATTCAGTATAAAGGTGTCGTCCAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAACTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAttt                                                                                                                            <  1:163459/141‑1 (MQ=37)
         aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTTAACGAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                                                          >  1:183015/1‑141 (MQ=255)
         aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                                                          >  1:348197/1‑141 (MQ=255)
          gTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATc                                                                                                                         >  1:502719/1‑141 (MQ=255)
            aTTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCAt                                                                                                                       <  1:174987/141‑1 (MQ=255)
             tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                                                      >  1:61814/1‑141 (MQ=255)
              ttAATGTAGAACTGATTCATTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGt                                                                                                                     <  1:519346/141‑1 (MQ=255)
              tgaaTGTAGAAGCTATTCAGTATAAAGCTTGTGGCGAAAAATGCTTACGTTATGAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGt                                                                                                                     <  1:179571/139‑1 (MQ=37)
                aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTAATTGAACAGGAGCTAAAAGAAAATTGTGATCTAATAAAATTAGAAGGTTCTGTTGCAAAGTAAAAGAATATAGCTAACAACTAATTTCTCTTGTCa                                                                                                                   >  2:384404/1‑141 (MQ=38)
                aaTGTAGAAGCTATTCAAGATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAATATTGTGATCTAATAAAAATAGAAGGTTATGTTGCAAAATAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                                                   >  2:182925/1‑141 (MQ=38)
                    tAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                                               <  1:184733/141‑1 (MQ=255)
                       aaGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                                            >  1:715998/1‑141 (MQ=255)
                        atcTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                           <  2:410365/139‑1 (MQ=255)
                        aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAACATAGAAGGTTCTGAAAAAAAAAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                           <  2:448134/141‑1 (MQ=38)
                        aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                           <  2:144333/141‑1 (MQ=255)
                        aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACCTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAACACAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                           <  2:55759/141‑1 (MQ=255)
                          cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                                         >  1:432990/1‑141 (MQ=255)
                          cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                                         >  2:417709/1‑141 (MQ=255)
                            aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                                                       >  1:675726/1‑141 (MQ=255)
                            aTTCAGTATAAAGTTTTTGGAGAAAAATGATTACTTGAACAAGATCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGGTGCAAAGTAAAAAAAATATATGTAACCACTAATTTATCTTGTCATTGTTCGCTTa                                                                                                        >  2:433780/1‑141 (MQ=255)
                              tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                                                     >  1:307313/1‑141 (MQ=255)
                               taGTATAAAGTTTTTGGCGAAAAATGATTACTTGAATAAGATTTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                                                    <  2:80172/140‑1 (MQ=255)
                               cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                                                    >  2:718520/1‑141 (MQ=255)
                                 gTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                                  >  1:546589/1‑141 (MQ=255)
                                  tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTCCTGTTGCAAAGTACAAAAATATAGCTAACAACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                 >  2:255557/1‑141 (MQ=255)
                                   ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTa                                                                                                <  2:749045/141‑1 (MQ=255)
                                    taAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTCACTTGCTAg                                                                                               >  1:772572/1‑141 (MQ=255)
                                       aGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAGTAATTTATCATGTCAGTGTTGCGTTAAATTGCTAGCat                                                                                            >  2:786794/1‑141 (MQ=37)
                                        gTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATTAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatg                                                                                           >  1:499625/1‑141 (MQ=255)
                                           tttGGCGAAAAATGACTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                        <  2:281577/141‑1 (MQ=255)
                                           tttGGCGAAAAAACATTACGTGAACAAGAGCTAAAATAAAGTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                        <  1:424521/141‑1 (MQ=255)
                                           tgtGGCGAAAAATAAATACTTGAAAAAGAGCTAAAATACAATTTTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGGAAAAAAAGATAGCTACCCACTATTTTACCATGACATTGTTCGCTTAACTTGCTAGCatgatg                                                                                        <  1:188553/139‑1 (MQ=21)
                                              ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                                                     >  2:687987/1‑141 (MQ=255)
                                               ttttttttttaTTACTTGAACAAGAGCTAAAATACAATTGGGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAGATATCTAACCACTAATTTATCATCTCAGTGTTCACTTAACTTGCTACCATGATGCTaa                                                                                    >  2:518972/11‑141 (MQ=18)
                                                cGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAAt                                                                                   >  2:376201/1‑141 (MQ=255)
                                                      aTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                                             <  1:543815/141‑1 (MQ=255)
                                                              ttGAATCAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCg                                                                     <  1:1260/141‑1 (MQ=255)
                                                                 aaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaa                                                                  >  2:164649/1‑141 (MQ=255)
                                                                     agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                              <  2:675726/141‑1 (MQ=255)
                                                                              acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTCATTTGCTTGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                   >  1:570813/15‑141 (MQ=11)
                                                                              acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                   >  1:67117/15‑141 (MQ=11)
                                                                               caaaagagtatcttaataaAAATGAGGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                  <  1:690303/128‑1 (MQ=11)
                                                                               aaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGGTAGTCACTAATATATCCTGTCAGTGTGCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCCGTAGATCTGAag                                                  <  2:398604/128‑1 (MQ=11)
                                                                                 aaatTGACATCTAATAAAAATAGAATGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                  <  1:474082/141‑1 (MQ=255)
                                                                                 aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                >  1:473655/12‑141 (MQ=11)
                                                                                 aaagactattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTCTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                <  1:378872/130‑1 (MQ=11)
                                                                                  aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATCTGAAgaga                                               >  1:477332/11‑141 (MQ=11)
                                                                                   agagtattttaataaAAATTGTGGTTCTGTTGCCAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                              <  2:10018/132‑1 (MQ=11)
                                                                                   agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTGATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGAAATTCTGAAAATCCGTAGATCTGAAGAGAc                                              <  2:522131/132‑1 (MQ=11)
                                                                                   agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                              <  1:11685/132‑1 (MQ=11)
                                                                                   agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                              <  2:150579/132‑1 (MQ=11)
                                                                                   agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                              <  2:780424/132‑1 (MQ=11)
                                                                                    gattattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                             <  1:251949/133‑1 (MQ=11)
                                                                                    gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTGATCACTAATATATCATGTCAGTGTTCGTTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                             <  2:514734/133‑1 (MQ=11)
                                                                                    gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                             <  2:93793/133‑1 (MQ=11)
                                                                                    gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                             <  1:365719/133‑1 (MQ=11)
                                                                                    gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                             <  1:192050/133‑1 (MQ=11)
                                                                                     tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                              <  1:122288/141‑1 (MQ=255)
                                                                                     tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                              >  1:398352/1‑141 (MQ=255)
                                                                                     tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                              <  1:329543/141‑1 (MQ=255)
                                                                                     tgtgAATTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                              <  1:712039/141‑1 (MQ=255)
                                                                                     ggtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGCAAAAACATATAGCTAACCACTAATTTATCATGTCAGTCTTCGCTTAACTTGCTAGCATGATGCTAATCTCGTGGCATGGCGAAAATCCGTGAATCTGCAGCGAc                                              >  2:588483/2‑141 (MQ=14)
                                                                                     agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGGTCTTAGGAGACCt                                            >  2:509622/8‑141 (MQ=11)
                                                                                     agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                            <  2:572279/134‑1 (MQ=11)
                                                                                     agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                            <  2:568759/134‑1 (MQ=11)
                                                                                     agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                            <  2:572424/134‑1 (MQ=11)
                                                                                     agtattttaataaAAATGATGGTTCTGGTGCAAAGTAAAAAATATAGCTAACCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                            <  2:143336/134‑1 (MQ=11)
                                                                                     agtattttaataaAAATGATAGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                            >  1:476811/8‑141 (MQ=11)
                                                                                      gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                             <  2:87037/141‑1 (MQ=255)
                                                                                      gtattttgataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                           <  2:751393/133‑1 (MQ=11)
                                                                                      gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                           <  1:563105/135‑1 (MQ=11)
                                                                                      gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                           <  2:311450/135‑1 (MQ=11)
                                                                                      gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCAGTTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTg                                           >  1:380189/7‑141 (MQ=11)
                                                                                       tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                            <  2:244772/141‑1 (MQ=255)
                                                                                       tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                          <  1:702764/136‑1 (MQ=11)
                                                                                       tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                          >  2:415452/6‑141 (MQ=11)
                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTGACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAtgaggactgagg                                        >  1:58427/4‑133 (MQ=11)
                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                        <  1:223809/138‑1 (MQ=11)
                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                        <  1:687879/138‑1 (MQ=11)
                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTCCTAGCATGATGCTAATTTCGTGGCATGGGGAAAACCCGTACATCTGAAGAGAACTGAgg                                        >  2:195721/4‑141 (MQ=11)
                                                                                          tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATGTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                       <  1:85120/139‑1 (MQ=11)
                                                                                          tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                       <  2:770750/139‑1 (MQ=11)
                                                                                          tttaataaAAATGATGGTGCTGTTGCAAAGTAAAAAATATAGCTCATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                       <  2:687952/139‑1 (MQ=11)
                                                                                           ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                      <  2:627242/140‑1 (MQ=11)
                                                                                           cTAATAAAAATAGAAGGTTCTGTAACAAAAAAAAAAAATATAGCTAACCACTATTTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                        <  2:435088/141‑1 (MQ=14)
                                                                                           ataataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                        <  2:397519/140‑1 (MQ=40)
                                                                                            taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGCTCTGAAGAGACCTGCGGTTc                                     <  1:510990/141‑1 (MQ=11)
                                                                                              ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                   >  1:322063/1‑141 (MQ=11)
                                                                                                aaaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATGTGAATAACCACTTATTACAGATTCCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                   >  1:186228/1‑141 (MQ=11)
                                                                                                  aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                 >  1:93595/1‑141 (MQ=35)
                                                                                                  aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGGTCtttt                                 >  1:148482/1‑141 (MQ=25)
                                                                                                   aaTAGGAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCGGTAGTGCTGAAGAGTCCTGCGGTTCttttt                                <  2:398652/141‑1 (MQ=11)
                                                                                                       gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGCAGAGACCTGCGGTTCTTTTtatat                            >  1:329383/1‑141 (MQ=14)
                                                                                                       gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGCGACCTGCGGTTCTTTTtatat                            >  2:352035/1‑141 (MQ=14)
                                                                                                        aaGGTTCTGTTGCAAAGTAACAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGCAAATCCGTAGAGCTGAAGAGACCTGGGGTTCTTGTtatata                           >  1:633601/1‑141 (MQ=11)
                                                                                                                                  aatatcaaaCCATTAATTTATCATGTCAGGGTTCGCTTAACCTGCTAGCATGATGCGAATGTCGTGTCATGGCGATAATCCGTAGATCGGAAGAGCCCTGCTGTTCTTTTTATATAGAGCGTAAATACACTCAATACCttt  <  2:612040/134‑1 (MQ=1)
                                                                                                                                           |                                                                                                                                   
CATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTT  >  CP000730/35712‑35981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: