Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 667,603 G→A 8.6% intergenic (+635/‑413) USA300HOU_0609 → / → adh phosphohydrolase/alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730667,6030GA 8.6% 215.4 / 8.3 81intergenic (+635/‑413)USA300HOU_0609/adhphosphohydrolase/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (25/49);  new base A (4/3);  total (29/52)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

ATGAAGGTATGTGGAAGCATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAG  >  CP000730/667465‑667743
                                                                                                                                          |                                                                                                                                            
atgaAGGTATGTAGAAGCATTTCAAAGAAGCTTTACAGCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATACATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTc                                                                                                                                            <  2:790796/141‑1 (MQ=255)
    aGGAATGAGGAAGCATTCATCTTATGCTTTACATCAAGCAGTTAATAAACAATCATTAACCAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAg                                                                                                                                        <  1:218800/141‑1 (MQ=255)
     ggTATGTGGAAGCATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAga                                                                                                                                       <  1:117564/141‑1 (MQ=255)
     ggTATGTGGAAGCATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGCCTTAga                                                                                                                                       <  1:117553/141‑1 (MQ=255)
        aTGTGGAAGCATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAgattg                                                                                                                                    >  1:749780/1‑141 (MQ=255)
             gAAGCATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATATCTTAGATTGATTgg                                                                                                                               <  1:618590/141‑1 (MQ=255)
             gAAGCATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTAATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATATCTTAGATTGATTgg                                                                                                                               <  2:45591/141‑1 (MQ=255)
             gAAGCATTCTTCTGATGCTTTACATAAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTgg                                                                                                                               <  1:227478/141‑1 (MQ=255)
             gAAGCAGTCTTCCGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTgg                                                                                                                               <  1:387642/141‑1 (MQ=255)
                 cATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGt                                                                                                                           >  1:682708/1‑141 (MQ=255)
                 cATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGt                                                                                                                           >  1:195365/1‑141 (MQ=255)
                   ttcttcTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGAAGttt                                                                                                                         >  2:459166/1‑141 (MQ=255)
                     tttctGATGCTTTACATCAAGCATTTAAATAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGttttt                                                                                                                       <  2:10790/140‑1 (MQ=255)
                     cttctGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGttttt                                                                                                                       >  2:394088/1‑141 (MQ=255)
                     cttctGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGttttt                                                                                                                       >  1:147645/1‑141 (MQ=255)
                          gATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAAt                                                                                                                  >  1:596889/1‑141 (MQ=255)
                              cTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAAttttt                                                                                                              >  2:244496/1‑141 (MQ=255)
                                ttACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAAttttttt                                                                                                            <  2:505823/141‑1 (MQ=255)
                                tgaCATCAAGCATTTAATTAAATATTAGAAACTAAAATTTTCGAATTAATTTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGGGTTTTTTTAAttttttt                                                                                                            <  2:133429/139‑1 (MQ=255)
                                  aCATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGtttttttttttttttttt                                                                                                          >  2:209648/1‑139 (MQ=255)
                                   cATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGaa                                                                                                         >  1:508738/1‑141 (MQ=255)
                                      cAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCTTGAGTTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGaaatt                                                                                                      <  1:343677/141‑1 (MQ=255)
                                      cAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGGTTGATTGGGAGTTTTTTTAATTTTTTTGaaatt                                                                                                      >  2:786502/1‑141 (MQ=255)
                                      cAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGaaatt                                                                                                      <  1:640333/141‑1 (MQ=255)
                                      aaaGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGaaatt                                                                                                      <  1:46796/140‑1 (MQ=255)
                                       aaGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGaaatta                                                                                                     >  1:278032/1‑141 (MQ=255)
                                          cATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGaaattaaat                                                                                                  <  1:174034/141‑1 (MQ=255)
                                            tttAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAaattaaatta                                                                                                <  2:52061/141‑1 (MQ=255)
                                            tttAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAaattaaatta                                                                                                <  1:33905/141‑1 (MQ=255)
                                            tttAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGACTGATTGGGAGTTTTTTTATTTGTTTTGAaattaaatta                                                                                                <  1:440614/141‑1 (MQ=255)
                                               aattcaATATTAGAAACTAAAATTTCCCAATCAATCTATAAAGATATGATTCATTTCTCACTGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATc                                                                                             <  1:264836/141‑1 (MQ=255)
                                                attaaATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCt                                                                                            >  2:678452/1‑141 (MQ=255)
                                                attaaATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTAAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCt                                                                                            >  2:333239/1‑141 (MQ=255)
                                                    aaTATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAg                                                                                        >  1:651471/1‑141 (MQ=255)
                                                    aaTATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAg                                                                                        >  2:499235/1‑141 (MQ=255)
                                                         tAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTtaat                                                                                   <  2:601933/141‑1 (MQ=255)
                                                         tAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTtaat                                                                                   <  2:220872/141‑1 (MQ=255)
                                                                    gctacccAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGTTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGa                                                                        <  2:586353/137‑1 (MQ=255)
                                                                    aTTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATTACGACGTAAATCGTGATGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTGTTTTGAAATTAAATTAATCTGTAGTCAATAAAAAATTTGa                                                                        <  1:516040/141‑1 (MQ=255)
                                                                    aTTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGa                                                                        <  2:246387/141‑1 (MQ=255)
                                                                      ttCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAAt                                                                      <  1:557486/141‑1 (MQ=255)
                                                                         ccAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATATCTTAGATTGATTGGGAGCTTTTCTCAACTTGTTGAACTTAAATTATTCTGTTGTTAATAAAAAATTTGAATatc                                                                   >  1:771520/1‑139 (MQ=255)
                                                                          cAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACt                                                                  <  1:243528/141‑1 (MQ=255)
                                                                             ttAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGac                                                               <  2:642640/141‑1 (MQ=255)
                                                                              tAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATATCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGaca                                                              >  2:145803/1‑141 (MQ=255)
                                                                                  cTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGTcacagt                                                          <  2:682708/141‑3 (MQ=255)
                                                                                  cTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACAtt                                                          <  1:184937/141‑1 (MQ=255)
                                                                                  cTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACAtt                                                          <  2:195365/141‑1 (MQ=255)
                                                                                      aaaGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACAtttttt                                                      <  1:733993/141‑1 (MQ=255)
                                                                                       aaGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACAttttttt                                                     <  2:771521/141‑1 (MQ=255)
                                                                                       aaGATATGATTAATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACAttttttt                                                     <  1:46582/141‑1 (MQ=255)
                                                                                       aaGATAGTATTCATGTCGCAAGGACGACGGAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATGTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACAttttttt                                                     <  2:2292/141‑1 (MQ=255)
                                                                                        aGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAGTTAATCTGTAGTTAATAAAAATTTTGAATAACTGACACAtttttttt                                                    >  2:716524/1‑140 (MQ=255)
                                                                                         gATATGATTCATTTCTCAATGACTACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGa                                                   <  2:578918/141‑1 (MQ=255)
                                                                                         gATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGa                                                   <  1:490934/141‑1 (MQ=255)
                                                                                         catatGATTCATTTCTCAATGACGACGTAAACCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATCAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGa                                                   <  1:657247/140‑1 (MQ=255)
                                                                                              gATTCATTTCTCAATGACGACGTACATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATa                                              >  2:133911/1‑141 (MQ=255)
                                                                                                 tCATTTCTCAATGACGACGTTAATCGTGAGGTGAAAATAATGTCATAGATTGATTGGGAGTTTTTTTAATTTTTTTTTTATTTATGTGATCTGTAGTTAATAAAAAAGTTGAATAACTGACACATTTTTTTGATCATAGCt                                           >  2:432494/1‑141 (MQ=255)
                                                                                                 tCATTTCTCAATGACGACGTAAATCGTGAGGTTAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTGTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCt                                           <  2:85553/141‑1 (MQ=255)
                                                                                                   tttttTCATTGATGACTGAAATCGTGAGGTGATAAGAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTTAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGCTCATAGCtat                                         <  2:419687/140‑1 (MQ=255)
                                                                                                   aTTTCTCAATGATGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCtat                                         <  2:56196/141‑1 (MQ=255)
                                                                                                           aTGACGACGTAAATCGTGAGGTGAAAATAATATCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTtg                                 >  1:14486/1‑141 (MQ=255)
                                                                                                            tGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTACTTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAGTTTGCATAACTGACACATTTTTTTGATCATAGCTATATACTTtgt                                >  2:392409/1‑141 (MQ=255)
                                                                                                            tGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTACTCTGTAGTTAATAAGAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACtttat                                >  1:3076/1‑139 (MQ=255)
                                                                                                              acgacgTAAATCGTGAGGTGAAAATAATATCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGa                              <  1:487048/141‑1 (MQ=255)
                                                                                                                 acgTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTGTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGaatt                           <  1:104165/141‑1 (MQ=255)
                                                                                                                  cgTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTTTTTTTTTTTTAATCTGTAGTTAATAAAACATTTGAATAACTGATAAATTTTTTTGATCATGGCtatatcgtcttttagtta                          >  2:640339/1‑128 (MQ=255)
                                                                                                                  cgTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGaatta                          >  1:13674/1‑141 (MQ=255)
                                                                                                                    tAAATCGTGAGGTGAAAATAATATCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTGATAAAGAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGaattaat                        >  1:573474/1‑141 (MQ=255)
                                                                                                                      aaTCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTc                      <  1:215938/141‑1 (MQ=255)
                                                                                                                      aaTCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAAATGACACGTTTTTTTGGTCATAGTTATATACTTtgaacactaatta                      >  2:160849/1‑130 (MQ=255)
                                                                                                                               gTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATTTTTTCTATATACTTTGTGAATTAATTCACATTataa             >  2:6094/1‑141 (MQ=255)
                                                                                                                                tGAAAATAATGTCTTAGATTGATTGGGAGTTTTGTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTataat            <  1:505231/141‑1 (MQ=255)
                                                                                                                                   aaaTAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag         <  2:651471/141‑1 (MQ=255)
                                                                                                                                   aaaTAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTCAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAag         <  1:532862/141‑1 (MQ=255)
                                                                                                                                       aatGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTg     <  2:147645/141‑1 (MQ=255)
                                                                                                                                       aatGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTg     <  1:135296/141‑1 (MQ=255)
                                                                                                                                       aatGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTg     <  1:16600/141‑1 (MQ=255)
                                                                                                                                       aatGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTg     <  1:394088/141‑1 (MQ=255)
                                                                                                                                        atGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGa    <  2:569427/141‑1 (MQ=255)
                                                                                                                                        atGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGa    <  2:673340/141‑1 (MQ=255)
                                                                                                                                          gTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGATAATAAAAAATTTGAATAAATGAAACATTTTTTTGATCTTAGCTATATACTTTGTGAATTAATTCACATTATAATAAGACTGaaa  >  2:139667/1‑140 (MQ=255)
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ATGAAGGTATGTGGAAGCATTCTTCTGATGCTTTACATCAAGCATTTAATTAAATATTAGAAACTAAAATTTCCCAATTAATCTATAAAGATATGATTCATTTCTCAATGACGACGTAAATCGTGAGGTGAAAATAATGTCTTAGATTGATTGGGAGTTTTTTTAATTTTTTTGAAATTAAATTAATCTGTAGTTAATAAAAAATTTGAATAACTGACACATTTTTTTGATCATAGCTATATACTTTGTGAATTAATTCACATTATAATAAGAGTGAAG  >  CP000730/667465‑667743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: