Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,997 T→C 30.1% T185T (ACA→ACG USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,9970TC30.1% 1203.4 / inf 1007T185T (ACA→ACGUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (377/323);  new base C (156/146);  total (535/472)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1010 total aligned reads displayed.

CATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTT  >  CP000730/35862‑36137
                                                                                                                                       |                                                                                                                                            
     cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc                                                                                                                                    >  2:735552/1‑141 (MQ=2)
      aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                                                   >  1:635328/1‑141 (MQ=9)
         gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg                                                                                                                                <  1:169088/141‑1 (MQ=11)
         gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                                                >  2:292952/1‑141 (MQ=17)
          ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                                                                               <  2:618002/141‑1 (MQ=255)
          ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                                                                               <  1:776870/141‑1 (MQ=32)
            cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                                                                             >  1:27881/1‑141 (MQ=32)
            cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGGTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                                                                             >  2:544905/1‑141 (MQ=17)
             gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACCCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                                                            <  1:463792/141‑1 (MQ=17)
             gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTgttgc                                                                                                                            >  1:287585/1‑137 (MQ=17)
             gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCACGGCGAAGACATGTAGCTCTGAAGAGACCTGGAAGTTTGCTTGGACAGGGCCTTTCAGCTCGCAAGACCTTTTAAAGTATTCTTTGCTGTATTGATGCTTTGATAc                                                                                                                            <  1:577290/141‑1 (MQ=2)
              tgtAACTTGCTAGTATGATGCTAATTTAGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                                                                           <  1:264788/139‑3 (MQ=11)
               ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                                                                                                          <  1:616740/141‑4 (MQ=255)
                 aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                                                        <  2:593046/141‑1 (MQ=32)
                   cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTATTTTTATATAGAGCGTAAATACACTCAATACCTTGTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtt                                                                                                                      >  2:127680/1‑140 (MQ=12)
                    ctGCTAGCACGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                     <  1:199251/140‑1 (MQ=12)
                     tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACGTGCGGTTCTTTTTATATAGAGCGTAAATACACACAATACCTTTTAAAGTATTCTTTGCTTTATTGGTACTTTGGTACCTAGtctt                                                                                                                    >  2:308555/1‑141 (MQ=11)
                      gCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                   <  2:176690/141‑1 (MQ=17)
                       cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttt                                                                                                                  >  2:634510/1‑140 (MQ=17)
                       atAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                                                                                  <  1:588165/140‑1 (MQ=255)
                        tAGCATGATGCTAATTTCGTTGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                 <  1:101260/141‑1 (MQ=17)
                        tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                 <  1:519790/141‑1 (MQ=17)
                          gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                                               >  1:453428/1‑141 (MQ=17)
                           cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAc                                                                                                              <  2:443698/141‑1 (MQ=255)
                           cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAc                                                                                                              >  1:536948/1‑141 (MQ=11)
                            atgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACt                                                                                                             >  2:787238/1‑141 (MQ=11)
                             tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttcttcgtt                                                                                                            >  1:252890/1‑137 (MQ=12)
                             tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTCCtt                                                                                                            >  2:107567/1‑141 (MQ=17)
                               atgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                                                                                                          <  1:92904/141‑1 (MQ=11)
                                tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                                                         <  1:113853/141‑1 (MQ=255)
                                tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                                                         >  2:48689/1‑141 (MQ=21)
                                   tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                      >  1:687294/1‑141 (MQ=34)
                                   tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTTTTACTTTAatat                                                                                                      >  1:356791/1‑141 (MQ=21)
                                   tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                      >  2:645213/1‑141 (MQ=25)
                                   tAATTTCGTGGCATGGCGAAAATCCGTAGATATGAAGTATCGGTCAGATCTTTTAATAAAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                                                                                      >  1:186271/1‑141 (MQ=11)
                                    aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGCGAACTACGGTTCTATTTATAGAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTACTACTTTAATATCTTGTCTTTCTTACCTTAATATg                                                                                                     >  2:279935/1‑141 (MQ=11)
                                       ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                                                  <  2:505935/141‑1 (MQ=37)
                                       ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTCTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                                                  <  2:262440/141‑1 (MQ=25)
                                       ttCGTGGAATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                                                  >  1:492283/1‑141 (MQ=255)
                                          gTGGCATGGGGAAAATCCGTATATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTACTCTTTTCCGTATCGATACTTTGATATCCTTTCTTTCTTACTTTTAGATCACGGTg                                                                                               >  1:127634/1‑141 (MQ=11)
                                          gTGGCATGGCGAAAATCCGTAGATCTGACGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                               <  1:548398/141‑1 (MQ=255)
                                           gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                              <  2:703159/140‑1 (MQ=255)
                                               aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                                                          <  1:564939/141‑1 (MQ=255)
                                               aTGGCGAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                         <  2:213914/141‑1 (MQ=255)
                                                 ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                                                        <  1:602166/138‑1 (MQ=255)
                                                 ggCGAAAATCCGTAGGTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTGGGTCGTACTTTACTCTGACGGTGATCTTGc                                                                                        <  2:522104/141‑1 (MQ=11)
                                                  gggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                       >  1:306003/3‑141 (MQ=255)
                                                  gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                       >  2:396297/1‑141 (MQ=37)
                                                      aaaGCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                   <  1:14880/141‑1 (MQ=255)
                                                        aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                 >  1:702312/1‑141 (MQ=255)
                                                         tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                                <  1:789993/141‑1 (MQ=255)
                                                               gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                          >  1:378361/1‑141 (MQ=255)
                                                               gATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                          <  1:626950/141‑1 (MQ=255)
                                                                 tCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATAACGGTGATCTTGCTCAATGAGGTTATTCa                                                                        >  2:319546/1‑141 (MQ=11)
                                                                  cTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                                                       <  1:441400/141‑1 (MQ=255)
                                                                   tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGATGTTATTCAGa                                                                      >  2:628722/1‑141 (MQ=255)
                                                                   tGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                      <  2:365698/141‑1 (MQ=255)
                                                                     aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTTTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACAGTGATCTTGCTCAATGAGGTTATTTAGatt                                                                    >  1:321893/1‑140 (MQ=11)
                                                                       ggtgccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTCCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                  <  2:3814/137‑1 (MQ=255)
                                                                       gagaCCTGCGGTGTCTTTTATATGGGGCGTAAATACACTCAATACCTTTTAAAGTAGTCTTTGCTGTATTGATACTTTGATACCTTGGCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                  <  1:656914/141‑1 (MQ=14)
                                                                        agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTGTTAGAGTATTCTTTGCTGGATTGATACTTTGATACAGTGTCTTGCTTCATTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCCGTTAttt                                                                 >  2:685057/1‑141 (MQ=11)
                                                                             tacGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGATg                                                            >  2:422772/3‑141 (MQ=255)
                                                                              acGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGCTACTTTGATAGCTTGTCTTTCTTACTTTAATATGACGGTGAGCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           >  2:676644/2‑141 (MQ=255)
                                                                                 tttCTTTTTATATTGAGCTCATATATCCTTTATTCCTTTTAATGTATTCTTTGTTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTAATCTTGCTCAATGAGGTTATTCAGATATTTCCATGTACa                                                        <  2:722377/140‑1 (MQ=11)
                                                                                     tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATCCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATGAGGTTATTCAGATATTTCGATGTACAATgg                                                    >  2:85608/1‑140 (MQ=255)
                                                                                     tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                                                    <  1:678465/141‑2 (MQ=255)
                                                                                      ttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggc                                                   <  1:709753/141‑3 (MQ=255)
                                                                                           tataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCATTGAGGTTATTCAGTTATTTCGATGTACAGTgtcagtca                                              >  1:223939/1‑141 (MQ=14)
                                                                                           tataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGCCa                                              >  1:72104/1‑141 (MQ=255)
                                                                                            ataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                             <  1:336776/141‑1 (MQ=255)
                                                                                             taGGGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                            <  1:391148/141‑1 (MQ=255)
                                                                                              agagCGTAAATACACTCAATACCTTTTAAAGTATTCGTTGCTGTATTGGTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCGTGCTCAATGGGGTTATTCAAATATTTCGATGTACAATggcggcccggt                                           >  2:220236/1‑131 (MQ=11)
                                                                                               gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                          >  1:136183/1‑141 (MQ=255)
                                                                                                tgtgTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                         <  2:352768/138‑1 (MQ=255)
                                                                                                agCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttt                                         >  1:424458/1‑141 (MQ=255)
                                                                                                     aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGATCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                    <  2:570813/141‑1 (MQ=255)
                                                                                                      aacactcgcAATAGCCTTTCACGTATTCTTTGGTGTATTGACACTTTGATACGTTGTCTGTCTTACCTTAATATGACGGTGATCTTGCTCAATGGTGTTATGCAGAAAATTCGATGTACAATGGTAGTCAGgtttaagttt                                   <  2:5370/133‑1 (MQ=255)
                                                                                                      aaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                   >  2:193749/1‑141 (MQ=255)
                                                                                                      aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACGGGTAGggttaagttt                                   >  2:340021/1‑141 (MQ=14)
                                                                                                      aaTACATTCAATACCTTTTAAAATTTTATATTTCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACATTTACAGTCAGgtttaatttt                                   <  2:754193/141‑1 (MQ=11)
                                                                                                      aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                   >  2:233978/1‑141 (MQ=255)
                                                                                                      aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                   >  2:159438/1‑141 (MQ=255)
                                                                                                       aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                  >  2:768960/1‑141 (MQ=255)
                                                                                                       aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCTGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                  >  2:467710/1‑141 (MQ=255)
                                                                                                       aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                  >  2:141682/1‑141 (MQ=255)
                                                                                                          cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATAGCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa                               <  1:735552/141‑1 (MQ=255)
                                                                                                            ctCAATACCTTTTAAAATATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                             >  1:404188/2‑141 (MQ=255)
                                                                                                              cAATACCTTTTATAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCtt                           <  2:656554/141‑1 (MQ=255)
                                                                                                                    cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTGTGCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                     >  2:434495/1‑141 (MQ=255)
                                                                                                                           aGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaac              >  1:50549/1‑139 (MQ=255)
                                                                                                                             tATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCa            >  2:585350/1‑141 (MQ=255)
                                                                                                                             tATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCa            >  2:474748/1‑141 (MQ=255)
                                                                                                                              aTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAGCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGCTTAAAAGCTTTAATTATTTTAGCCAt           >  1:476099/1‑141 (MQ=14)
                                                                                                                              aTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACAGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTATCCAt           >  1:242894/1‑141 (MQ=11)
                                                                                                                               ttCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAtt          >  2:454582/1‑141 (MQ=255)
                                                                                                                               ttCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTACATGGGGTTAGTCAAATATTTCGATGTACAATGACAGTCAGGGCTAGGTTTAAAAGCTTTAATTATTTTAGCCAtt          >  2:406874/1‑141 (MQ=255)
                                                                                                                                 ttttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc        <  1:368798/140‑1 (MQ=255)
                                                                                                                                  tttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTACCCATTGCt       >  1:66758/1‑141 (MQ=255)
                                                                                                                                     gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc    >  2:247836/1‑141 (MQ=255)
                                                                                                                                     gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc    >  1:755127/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                                            
CATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTT  >  CP000730/35862‑36137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: