Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,015 C→T 29.9% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0150CT29.9% 1531.7 / inf 1111R179R (AGG→AGAUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (388/380);  new base T (182/149);  total (579/532)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 1134 total aligned reads displayed.

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155
                                                                                                                                            |                                                                                                                                            
  ttAACTTGCCAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                                                                            <  2:190861/141‑1 (MQ=17)
     aCTTGCTAGCATGATGCAAAAACGGCGGCAGGGCGAAAATACGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATACATTGt                                                                                                                                         >  1:813886/1‑141 (MQ=11)
      cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtc                                                                                                                                        >  1:961743/1‑141 (MQ=255)
          cTAGCATGATTCTAATTTCGAGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                    <  2:474601/141‑1 (MQ=12)
          cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                    <  1:763276/141‑1 (MQ=32)
          cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                    <  1:723792/141‑1 (MQ=17)
             gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTa                                                                                                                                 >  1:611203/1‑141 (MQ=11)
                agagtcTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                                                              <  1:703362/136‑1 (MQ=11)
                 tgtgtgacttgcttGGCATGGGGAAAACCCGTAGATCCGAAGCGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATCCTTTGATATCTTGTCTTTCTTACttt                                                                                                                             <  2:1007142/128‑1 (MQ=11)
                  atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATCCTTTGATACCTTGTCTTTCTTACTTTa                                                                                                                            >  2:928264/1‑141 (MQ=21)
                   tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                                                                                                                           >  2:676413/1‑141 (MQ=25)
                       aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCTATACCTGTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                                       <  1:979066/141‑1 (MQ=255)
                       aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATGTCTTTTCTTTCTTACTTTAATATg                                                                                                                       >  2:175152/1‑141 (MQ=255)
                       aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                                       >  2:494693/1‑141 (MQ=21)
                           tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACgg                                                                                                                   >  1:531786/1‑141 (MQ=255)
                            cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                                                                                                  <  1:293894/141‑1 (MQ=255)
                             gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTTTCTTTCTTACTTTAATATGACGGTg                                                                                                                 >  1:172562/1‑141 (MQ=11)
                             gTGGCATGGCGACAATCCGTAGATCTGAAGAGACCTTCGGTTCTTTTTATATGGAGCGTAAAGACACTCCATACCTTTTAAAGTATTTTTTGCTGTATTGGTACTTTCATACCTTGTCTTTCTTACTGTAATATGACGTTg                                                                                                                 >  1:479444/1‑141 (MQ=11)
                             gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                 <  2:1037564/141‑1 (MQ=255)
                              tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAACTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                                                >  2:657843/1‑141 (MQ=255)
                                   agccGAAACTGCGTGGATATGAAGAGACAAGCGGTTCTTACTCCAGAGAGCATAAAGACATTCAATACCTATTAAAGTATTAATTGCTGTATTGATACTTTGATACCTTATCTTTCTTACTTTAATATGACGGTGAGCTTg                                                                                                           >  2:89310/4‑141 (MQ=255)
                                         aaaTCCGTAGATCTGAAGATACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     <  1:242969/141‑1 (MQ=255)
                                             ccGTAGATCTGAAGAGAACTGCGGTTCTTTTTATATAGAGCGTAAATACATTCACTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTGATCTTGCTCCATgggg                                                                                                 >  2:748728/1‑141 (MQ=11)
                                              cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                                                >  1:678889/1‑141 (MQ=255)
                                              cGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTACAGTATTCTTTGCCGTATTGCTAATTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATCAGGt                                                                                                >  1:1054984/1‑141 (MQ=11)
                                                  gATCTGAAGAGTCCTGCGGTTCTTTTTATATAGGGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                                            <  2:871732/141‑1 (MQ=255)
                                                  gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTCATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                                            <  1:735854/141‑1 (MQ=38)
                                                     cTGAAGAGACCTGCGGTTATTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTCTAATATGACCGTGATCTTGCTCAATGAGGTTATTCAg                                                                                         >  2:420300/1‑141 (MQ=255)
                                                      tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                                        <  2:341435/141‑1 (MQ=255)
                                                        aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTATTATGACGGTGATCTTGCTCACGACGGTTATTCAGata                                                                                      >  2:443419/1‑141 (MQ=11)
                                                        aaGAGACCTGCGGTGCTGTTTATATAGAGCGTAAATACAATCAATACCATTTAAAGTATTCTTTGCTGTATTGATACTTTGATACGTTGTGTTTTTTAGTTTGATATGACGTTGATCTTGTTCACTGCGATTATTCTGatt                                                                                      >  2:409349/1‑140 (MQ=11)
                                                              ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                                                <  2:23005/141‑1 (MQ=38)
                                                               cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                                                               <  1:622743/141‑1 (MQ=255)
                                                                   ggttcatgtgaaagagagCGAAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAATTTACTCTAAAGGTGAGCTTGGTCAATGAGGTTATTCAGATATTTCGATGTAc                                                                           >  2:1059059/15‑141 (MQ=11)
                                                                     ttCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGTGACTTTGTTACCTTGTCTTTCTTACTTTAGTATGACGGTGATCTTGGCGAATGAGCTTATTCAGATATTTGGATGTACaa                                                                         >  2:963197/1‑141 (MQ=11)
                                                                      tCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                        >  1:420320/1‑141 (MQ=255)
                                                                       ttttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTTCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                                                                       <  2:727442/140‑1 (MQ=255)
                                                                        tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                                      <  1:402879/141‑1 (MQ=255)
                                                                        tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                                                                      <  1:897988/141‑2 (MQ=255)
                                                                          tttATATTGAGCGTAAATACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggca                                                                    <  1:331398/141‑4 (MQ=255)
                                                                          tttATATAGAGCGTAAATACACTCAAAACCTTTTAAAGTATTCATTGCTGTATTGAGACTTTGGTACCTTGTCTTTCTTACTTTCATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCTATGTACAATGGCa                                                                    <  2:519942/141‑1 (MQ=11)
                                                                           ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                                                                   >  2:274708/1‑141 (MQ=255)
                                                                             atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCGCGCGCAGTGAGGTTATTCAGATATTTCGATGTACCATGGCAGTc                                                                 >  1:853121/1‑141 (MQ=14)
                                                                              tataGCGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTCTTCAGATATTTCGATGTACAATGGCAGTCa                                                                <  1:502452/141‑1 (MQ=255)
                                                                              tataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCa                                                                <  2:769782/141‑1 (MQ=255)
                                                                                   ggcttAAATACTCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttt                                                           <  1:377611/137‑1 (MQ=255)
                                                                                    gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttc                                                          >  2:108361/1‑140 (MQ=255)
                                                                                    gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCGGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                                          <  1:57550/141‑1 (MQ=255)
                                                                                     cGTAAATACACTCAATACCTTTTAAAGGATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGgtttaa                                                         <  2:785434/141‑1 (MQ=255)
                                                                                      gTAAATACACTCAATACCTTTTACAGTATTCTTCGCTGTAGTTATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        <  1:202615/141‑1 (MQ=14)
                                                                                      gTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        >  2:206027/1‑141 (MQ=255)
                                                                                        aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                                      <  2:642263/141‑1 (MQ=255)
                                                                                         aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                     <  2:989545/141‑1 (MQ=255)
                                                                                          aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  2:915713/1‑141 (MQ=255)
                                                                                          aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                    >  2:610134/1‑141 (MQ=255)
                                                                                          aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTAAGgtttaagttta                                                    >  2:298596/1‑141 (MQ=255)
                                                                                           tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATCGTTTTGCTGATCATTAGATTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTAAGgtgtaaggttaa                                                   >  1:260104/1‑141 (MQ=11)
                                                                                           tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGACCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                   >  1:878593/1‑141 (MQ=255)
                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                   >  1:797010/1‑141 (MQ=255)
                                                                                           tACACTCAATACCTTTTAAAGTATTCTTCGCTGTATTGATACTTTGATACCTTGCCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGggttaagtttaa                                                   >  2:59210/1‑141 (MQ=14)
                                                                                            acacTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                  <  1:1013421/141‑1 (MQ=255)
                                                                                             cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaag                                                 >  1:359510/4‑140 (MQ=255)
                                                                                             cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                 >  1:31360/4‑141 (MQ=255)
                                                                                             cATTCAATACCTTTTCAAGTATTCTGTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa                                                 >  1:552386/1‑141 (MQ=255)
                                                                                             cATTAAATACCTCTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa                                                 >  1:780092/1‑141 (MQ=255)
                                                                                              actCAATACCTTTTAAAGTATTCTTTGCTGTATTGACACTTTCATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAg                                                <  1:514785/139‑1 (MQ=255)
                                                                                                tCAATACCTTTTAAAGTAGTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCt                                              <  2:319146/141‑1 (MQ=255)
                                                                                                    tACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTaa                                          >  1:215306/1‑141 (MQ=255)
                                                                                                     aCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                        >  1:467833/1‑110 (MQ=255)
                                                                                                     aCCTTTTAAAGTATTCGTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                         <  2:697389/141‑1 (MQ=255)
                                                                                                       cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATATCAAGTCTTTCTTCCGTGGATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAttc                                       >  1:1093398/1‑140 (MQ=14)
                                                                                                        ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                                      >  2:852298/1‑141 (MQ=255)
                                                                                                         tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACt                                     >  2:376912/1‑141 (MQ=255)
                                                                                                         tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGACATCCCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACt                                     <  1:650516/141‑1 (MQ=255)
                                                                                                          ttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                                    <  1:184885/141‑1 (MQ=255)
                                                                                                           tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGTCAGTCAGGTTTAAGTTTGAAAGCTTTAATTACttt                                   >  2:155832/1‑141 (MQ=255)
                                                                                                           tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                   >  2:804771/1‑141 (MQ=255)
                                                                                                                  ttCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt                            <  2:464916/141‑1 (MQ=255)
                                                                                                                     tttGCTGTATTGATACTTTTACACCTTGTCTTCCTGACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt                         <  1:137464/141‑1 (MQ=14)
                                                                                                                       tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTAc                       >  1:436843/1‑141 (MQ=40)
                                                                                                                        gccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTCTTCAGATATTCCGATGTACAATGACAGTCCGGTTTTACGTTAAAACCTTTATTTTTTTTACCCATTGCTGcc                      >  2:57606/4‑141 (MQ=14)
                                                                                                                         cTGTGTTGATTCTTTGGTGCCTTGTCTTTCTTACTTGAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTTTGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                     <  1:931015/141‑1 (MQ=14)
                                                                                                                         cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCt                     >  1:154891/1‑141 (MQ=255)
                                                                                                                           gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTc                   >  1:638380/1‑141 (MQ=255)
                                                                                                                            tATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCg                  <  1:1076437/141‑1 (MQ=255)
                                                                                                                            tATTGATACTTTGATAACTTGTCTTTCTTGCTTTAATATGAAGGTGATCTTGATCAATGAGGTTATTCAGATATTTCGATGTACAATGAAAGTAAGGCATAAGTTTAAAAGCTTTAATTTTTTTAGCCCTTGCTGCCtatg                  >  2:942150/1‑138 (MQ=14)
                                                                                                                             tttGAAACTTTGATATCTTGTCTTTCTTACTTTAATAAGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAGGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGt                 <  2:131425/140‑1 (MQ=14)
                                                                                                                              ttGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGtt                <  2:352892/141‑1 (MQ=255)
                                                                                                                              ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGtt                >  2:254247/1‑141 (MQ=255)
                                                                                                                                  tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAg            <  1:1001470/141‑1 (MQ=40)
                                                                                                                                  tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAg            >  1:115247/1‑141 (MQ=255)
                                                                                                                                    cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGTTTTAATTACTTTAACCATTGCTACCTTCGTTAAAGGt          >  2:625144/1‑141 (MQ=255)
                                                                                                                                      ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        <  1:45816/141‑1 (MQ=255)
                                                                                                                                      ttGATACCTTGTCTTTCTTACCTTAACATGACGGTGATCTTGCTCAATCAGGTTCTTCAGACATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        <  2:827723/141‑1 (MQ=11)
                                                                                                                                         aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCGGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg     >  1:347779/1‑141 (MQ=255)
                                                                                                                                         aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg     >  1:1028724/1‑141 (MQ=255)
                                                                                                                                          tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa    <  1:592624/141‑1 (MQ=255)
                                                                                                                                           aCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAt   >  2:111962/1‑141 (MQ=255)
                                                                                                                                           aCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAt   >  1:829493/1‑141 (MQ=255)
                                                                                                                                            ccTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATc  >  1:225568/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: