Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,373,506 A→T 7.3% N90K (AAT→AAA USA300HOU_2234 ← NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,373,5060AT 7.3% 311.3 / 8.9 110N90K (AAT→AAAUSA300HOU_2234NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base A (45/53);  new base T (5/3);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 113 total aligned reads displayed.

TAAGGAATTGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCT  >  CP000730/2373366‑2373641
                                                                                                                                             |                                                                                                                                       
tAAGGAATTGCATTAATAATCACGCCCCTAAACCCTCAGACGGTTAATATCGCAAAGAATATACCTGAGAATAAACCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCa                                                                                                                                         <  1:298637/141‑1 (MQ=255)
tAAGGAATTGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCa                                                                                                                                         <  1:589128/141‑1 (MQ=255)
 acggAACTGCATTAATAATAGCTTCCCTAACCCCTGTGACCGGTAATATCGCAAAGAACATACCTGAGAATAAAACGCCTGGTAAACCAACTTGCCAAGTAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCAt                                                                                                                                        <  2:306279/139‑1 (MQ=255)
 aacgaactccATTAATAATAACTTCCCTACACCCTTTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCAt                                                                                                                                        <  2:862962/132‑1 (MQ=255)
 aaGGAATTGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCAt                                                                                                                                        <  1:976084/141‑1 (MQ=255)
   agAATTTCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATAGACCTGAGAATAAAAAGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCAttt                                                                                                                                      <  2:131/140‑1 (MQ=255)
       ttGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATc                                                                                                                                  <  1:958272/141‑1 (MQ=255)
        tGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATcc                                                                                                                                 >  1:841802/1‑141 (MQ=255)
        tGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATcc                                                                                                                                 >  1:111212/1‑141 (MQ=255)
         gCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATccc                                                                                                                                >  2:648069/1‑141 (MQ=255)
         gCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCTTTTAATccc                                                                                                                                >  2:834360/1‑141 (MQ=255)
          cATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCACTTAATCCCa                                                                                                                               <  1:1051692/141‑1 (MQ=255)
               ataataaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATCTACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAACGCATTTAATCCCATACCt                                                                                                                          >  2:170222/1‑141 (MQ=255)
               ataataaCTTCCCTAAAAACAAAGACAATTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTACCAAGGAATACCCATGGTTAACACAACACTAAATGCAAAGAATGCATTTAATCCCATAccc                                                                                                                          >  2:784354/1‑140 (MQ=255)
                aaataaCCTCCCTAAACGCTGTGGCGGTTATGATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATAGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTg                                                                                                                         <  2:704390/140‑1 (MQ=255)
                   taaCTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTg                                                                                                                      >  1:427580/1‑141 (MQ=255)
                               aCCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCACTTg                                                                                                          <  2:11900/141‑1 (MQ=255)
                               aCCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTg                                                                                                          <  1:512357/141‑1 (MQ=255)
                                cccTGTTACAGTTCATATCCCAAAGAATTTAACTGAATATCTGTCGCCTGGTTAGACAACTTGCCAATAAATACCTCTGTATCACACAACAGTCAATGCAAAGAATGCATTTAATGTCTTCCATTGTGCTAACGCAATTgg                                                                                                         <  2:394214/141‑1 (MQ=255)
                                cccTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTgg                                                                                                         <  1:720355/141‑1 (MQ=255)
                                cccTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTgg                                                                                                         <  2:707948/141‑1 (MQ=255)
                                cccTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATCTAATCCCATACCTGGTGCTAACCCAATTgg                                                                                                         >  2:432819/1‑141 (MQ=255)
                                      gACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATAttt                                                                                                   >  1:425386/1‑141 (MQ=255)
                                       aCGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATACAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATAtttg                                                                                                  >  2:13698/1‑140 (MQ=255)
                                        cGGTTAATATCGCAAAGAATATACCTGAGAATGAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATACAAAGAATGCATTTAATCCCATACCTGGTGCTACCGCATTTGGATATTTAg                                                                                                 >  1:176349/1‑141 (MQ=255)
                                        cGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAg                                                                                                 >  1:372400/1‑141 (MQ=255)
                                        cGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAg                                                                                                 >  1:597059/1‑141 (MQ=255)
                                             aaTATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCACGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGAGCTAACGCAATTGGATATTTAGCTAtt                                                                                            >  1:764759/1‑141 (MQ=255)
                                             aaTATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATATAATCCCATACCTGGTGCGAACGCAATTGGATATTTAGCTAta                                                                                            >  2:8117/1‑140 (MQ=255)
                                             aaTATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATAGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGAGATAACGCAATTGGATATTTAGCTAtt                                                                                            >  2:494478/1‑141 (MQ=255)
                                                 tCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGcc                                                                                        <  1:182158/141‑1 (MQ=255)
                                                 tCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGcc                                                                                        <  1:273304/141‑1 (MQ=255)
                                                 tCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGcc                                                                                        <  1:380131/141‑1 (MQ=255)
                                                  cGCAAAGAATATACCTGAGAATAAACCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGccc                                                                                       <  1:359363/141‑1 (MQ=255)
                                                  cGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGGATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGCGCTAACGCAATTGGATATTTAGCTATTAGccc                                                                                       <  1:973199/141‑1 (MQ=255)
                                                     aaaGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCAt                                                                                    <  1:657275/141‑1 (MQ=255)
                                                      aaGAATATACCTGAGAATAAAACGCCGGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATg                                                                                   <  2:892738/141‑1 (MQ=255)
                                                        gAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGaa                                                                                 <  2:127307/141‑1 (MQ=255)
                                                          atatACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATa                                                                               >  2:92899/1‑141 (MQ=255)
                                                          atatACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATa                                                                               >  2:261178/1‑141 (MQ=255)
                                                          atatACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATa                                                                               >  1:1076900/1‑141 (MQ=255)
                                                          atatACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATa                                                                               >  1:565334/1‑141 (MQ=255)
                                                           tataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGACTAg                                                                              >  1:230048/1‑141 (MQ=255)
                                                           tataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAg                                                                              >  1:614951/1‑141 (MQ=255)
                                                            ataCCTGAGACTAAAACGCCTGTTAAACGAAGTTGCCAAGGAATACCCATGGTTAGCACAACAGTAAATGCAAAGAAGGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGc                                                                             >  2:533460/1‑141 (MQ=255)
                                                            ataCCTGAGAATAAAACGCCTGTTAAACCACCTTGCCAAGGAATACCCATGGTTACCACAACAGTAACTGCCAAGAATGCATTTAATCACATACCTGGTGCTCACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGc                                                                             >  2:337658/1‑141 (MQ=255)
                                                            ataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACCCGACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCGTGAATAGc                                                                             >  1:835713/1‑141 (MQ=255)
                                                            ataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGc                                                                             >  2:413299/1‑141 (MQ=255)
                                                            ataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGc                                                                             >  1:1068123/1‑141 (MQ=255)
                                                            ataCCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGc                                                                             >  1:232238/1‑141 (MQ=255)
                                                                     aaTAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACa                                                                    >  2:165388/1‑141 (MQ=255)
                                                                     aaTAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACa                                                                    >  1:401990/1‑141 (MQ=255)
                                                                       tAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACaaa                                                                  <  1:1052658/141‑1 (MQ=255)
                                                                         aaaCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGGAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAAtg                                                                >  2:783003/1‑141 (MQ=255)
                                                                           aCGCCTGTTAAACCAACTTGCNANAGANTACCCATGGTTAACTACAAGCTCGATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCGTACAAAtgct                                                              <  1:800061/141‑1 (MQ=255)
                                                                           aCGCCTGTTAAACCAACTTGCCAAGGAATACCTATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCAAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAAtgct                                                              <  1:738228/141‑1 (MQ=255)
                                                                           aCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAAtgct                                                              <  1:888520/141‑1 (MQ=255)
                                                                           aCGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAAGGTAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAAtgct                                                              <  2:607594/141‑1 (MQ=255)
                                                                                 gTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAAt                                                        <  1:964371/141‑1 (MQ=255)
                                                                                  ttAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATg                                                       >  1:494401/1‑141 (MQ=255)
                                                                                    aaaCCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCa                                                     >  1:470437/1‑141 (MQ=255)
                                                                                         aaCTTGCCAAGGACTACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGc                                                <  1:11405/141‑1 (MQ=255)
                                                                                         aaCTTGCCAAGGAATACCCATGGTTAACACAAACGTTAATGCGAAGAATGCGTTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGc                                                <  1:1032965/141‑1 (MQ=255)
                                                                                           cTTGCCAAGGAATCCCCAGGGTTAACACAACGGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTa                                              <  2:496920/141‑1 (MQ=255)
                                                                                            ttGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTTCAAATGCTGATAATGCAGTCGCTAc                                             <  1:554395/141‑1 (MQ=255)
                                                                                             ttccAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCATTAATATACTGCTAATGCAGTCGCTACa                                            <  2:1068840/139‑1 (MQ=255)
                                                                                             tGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCAAACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCGGCTAATGCAGTCGCGACa                                            >  1:665446/1‑141 (MQ=255)
                                                                                               ccAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGGGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATgaaaaaaaaaaaaa                                          <  2:647891/141‑14 (MQ=255)
                                                                                                     aaTACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGc                                    <  2:465075/141‑1 (MQ=255)
                                                                                                       tACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCAc                                  <  1:165388/141‑1 (MQ=255)
                                                                                                       tACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCAc                                  <  2:401990/141‑1 (MQ=255)
                                                                                                       tACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCAc                                  >  1:954015/1‑141 (MQ=255)
                                                                                                           cATGGTTAACACAACAGTAAATGCAAAGAATGCACTTCATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTg                              <  2:1093504/141‑1 (MQ=255)
                                                                                                            aTGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTgg                             <  1:894728/141‑1 (MQ=255)
                                                                                                             tGGTTAACACCACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATggaccaaggtg                            <  1:579945/141‑10 (MQ=255)
                                                                                                             tGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATggaccaaggtg                            <  1:841058/141‑10 (MQ=255)
                                                                                                             tGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATggaccaaggtg                            <  2:696066/141‑10 (MQ=255)
                                                                                                             tGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGt                            <  2:614951/141‑1 (MQ=255)
                                                                                                                   acacAACAGTAAATGCAAAGATTTCGTTTTATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAttt                      >  1:535903/1‑141 (MQ=255)
                                                                                                                   acacAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAttt                      <  1:219593/141‑1 (MQ=255)
                                                                                                                   acacAACAGTAAATGCAAAGAATGCATTTAAGCCCAAACCTGGTGCTAACGCAATTGGATACTTAGCTATTAGCCCCAGGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCAttt                      <  1:278745/141‑1 (MQ=255)
                                                                                                                      acaacaGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTc                    >  1:934777/1‑141 (MQ=255)
                                                                                                                       caacaGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCa                   <  1:261178/141‑1 (MQ=255)
                                                                                                                        aacaGTAAATGCCAAGAATGCATTTAATCCCATCCCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCat                  <  2:465601/141‑1 (MQ=255)
                                                                                                                        aacaGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCat                  <  1:846962/141‑1 (MQ=255)
                                                                                                                         acaGTAAATGCAAAGAATGCTTTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCatt                 >  2:339230/1‑140 (MQ=255)
                                                                                                                         acaGTAAATGCAAAGAATGCTGTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCCTTTTCata                 >  1:569701/1‑141 (MQ=255)
                                                                                                                         acaGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCata                 >  1:1089379/1‑141 (MQ=255)
                                                                                                                         acaGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCata                 >  1:96141/1‑141 (MQ=255)
                                                                                                                             tAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATAtctt             <  2:874370/141‑1 (MQ=255)
                                                                                                                             tAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAAACAAAAAAAAAATGGCACCTTGGTCCATTTTCATAtctt             <  2:917226/141‑1 (MQ=255)
                                                                                                                                aTGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTg          <  1:466515/141‑1 (MQ=255)
                                                                                                                                aTGCAAAGAAGGCATTTAATCCCATGGCGTGGGCTGACGCAATTGGATATTTAGGTATTAGCCCCATGAATAGCGAGCCTACAAGTGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTg          <  2:719886/141‑1 (MQ=255)
                                                                                                                                    aaaGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTGCATATCTTCTGATAc      >  1:413453/1‑141 (MQ=255)
                                                                                                                                      aGAATGCTTTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGc    <  1:648069/141‑1 (MQ=255)
                                                                                                                                      aGAATGCTTTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGc    <  1:962291/141‑1 (MQ=255)
                                                                                                                                      aGAATGCTTTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGc    <  1:834360/141‑1 (MQ=255)
                                                                                                                                        aaTGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGGTACGCCt  >  1:324816/1‑141 (MQ=255)
                                                                                                                                        aaTGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCt  >  1:21206/1‑141 (MQ=255)
                                                                                                                                        aaTGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAAGGCAGTCGCTACAAAAATGGCAACTTGGTCGTGTATCATAACTTCTGATACGCCt  >  1:197612/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                       
TAAGGAATTGCATTAATAATAACTTCCCTAAACCCTGTGACGGTTAATATCGCAAAGAATATACCTGAGAATAAAACGCCTGTTAAACCAACTTGCCAAGGAATACCCATGGTTAACACAACAGTAAATGCAAAGAATGCATTTAATCCCATACCTGGTGCTAACGCAATTGGATATTTAGCTATTAGCCCCATGAATAGCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCT  >  CP000730/2373366‑2373641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: