Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,575,960 C→T 6.0% V33I (GTT→ATT)  USA300HOU_2436 ← glycine betaine/choline ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,575,9600CT 6.0% 292.2 / 5.1 103V33I (GTT→ATT) USA300HOU_2436glycine betaine/choline ABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (50/45);  new base T (3/3);  total (54/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

Only 100 of 108 total aligned reads displayed.

TTTTACCAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGACATTAGTAGTGT  >  CP000730/2575822‑2576099
                                                                                                                                          |                                                                                                                                           
ttttACCAACCCCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGcaacaa                                                                                                                                           <  1:6599/141‑1 (MQ=255)
ttttACCAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGcaacaa                                                                                                                                           <  1:874711/141‑1 (MQ=255)
ttttACCAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGcaacaa                                                                                                                                           <  1:85320/141‑1 (MQ=255)
  ttACCAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAatg                                                                                                                                         <  2:511745/141‑1 (MQ=255)
    aCCAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAatgat                                                                                                                                       >  1:635865/1‑141 (MQ=255)
    aCCAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGATGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAatgat                                                                                                                                       >  1:624519/1‑141 (MQ=255)
    aCCAACACCAAAAAGCGGTATCATAATAGCAAGGACAGCTGGTGATGGAATTGTTTGTAAGACTCCTGTCACAGTTAATACAATAGTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAatgat                                                                                                                                       <  2:120024/141‑1 (MQ=255)
      cAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATgg                                                                                                                                     >  1:71605/1‑141 (MQ=255)
      cAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACAACTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTAATGCACGCATGATgg                                                                                                                                     >  2:918540/1‑141 (MQ=255)
        acacCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGTTAATTAAATGCCTATAGGTACTGCAACCATGATGTCa                                                                                                                                   >  2:574404/1‑141 (MQ=255)
        acacCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTCTTGGAATAGGTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATCCCTATAGGTACTGCCACAatgatgcaa                                                                                                                                   >  2:1009343/1‑138 (MQ=255)
        acacCAAAAATCGGTATCATAATAGCAAGTACAGCCAGTGTTGGAATAGTTTGTAAGACAACTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTGCTGCAACAATGATGGCa                                                                                                                                   >  2:532943/1‑141 (MQ=255)
         cacCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCaa                                                                                                                                  >  2:679408/1‑141 (MQ=255)
           ccAAAAATCGGTATCATAATAGCAAGTACCGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGt                                                                                                                                <  1:466511/141‑1 (MQ=255)
           ccAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAATAATGATGGCAAGt                                                                                                                                >  2:521011/1‑141 (MQ=255)
           ccAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAATAATGATGGCAAGt                                                                                                                                >  2:513984/1‑141 (MQ=255)
            cAAAAATCGGTATCAAAATAGCAAGTACAGCCAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGta                                                                                                                               <  2:528950/141‑1 (MQ=255)
              aaaaTCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGtaat                                                                                                                             <  1:861510/141‑1 (MQ=255)
               aaaTCGGTATCATAATAGCAAGTACAGCTCGGGTTGGAATAGTTTGTAAGACACCTGCCACAGGTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGtaata                                                                                                                            >  2:372093/1‑141 (MQ=255)
                   ccgTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCCCAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCAATAGGTACTGCAACAATGATGGCAAGTAATAATGc                                                                                                                        <  2:357261/139‑1 (MQ=255)
                   cGGAATCATAATAGCAAGTACAGCTAGTGTGGGAAGAGTTTGTAAGACACCTGCCACGGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGc                                                                                                                        <  2:582279/141‑1 (MQ=255)
                        tCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCCACTTTGTGGGCAAGTAATAATGCCACta                                                                                                                   <  1:265364/141‑1 (MQ=255)
                        tCATAATAGCAAGTACAGCATGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAGTGATGGCAAGTAATAATGCCACta                                                                                                                   <  1:703567/141‑1 (MQ=255)
                           taataGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACtatag                                                                                                                >  1:396299/1‑141 (MQ=255)
                              taGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACtatagaaa                                                                                                             >  1:544911/1‑141 (MQ=255)
                                          cTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGtt                                                                                                 <  1:600475/141‑1 (MQ=255)
                                            aGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCACGTAATAATGCCACTATAGAAATATAGAAATGTTca                                                                                               >  1:284089/1‑140 (MQ=255)
                                            aGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGttgc                                                                                               >  1:1077589/1‑139 (MQ=255)
                                             gtgtTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATAGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCa                                                                                              >  1:212587/1‑141 (MQ=255)
                                               gtTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGGAATATAGAAATGTTCCAgt                                                                                            >  1:233749/1‑141 (MQ=255)
                                               gtTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAgt                                                                                            <  2:536782/141‑1 (MQ=255)
                                                    aaTAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGtttt                                                                                       >  2:707025/1‑141 (MQ=255)
                                                    aaTAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGtttt                                                                                       >  1:836460/1‑141 (MQ=255)
                                                    aaTACTTTGTAAGACACCTGCCACAGCTAATACAAAATTGGCAGTTCGCTTTGTTTTTGATAATAACATGCCTATAGGTACTGCAACACTGATGGCCAGTAATAATGCCACTATAGAAATATAGACATGTTCCAGGGtttt                                                                                       >  2:287411/1‑141 (MQ=255)
                                                     aTAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTc                                                                                      <  2:111485/141‑1 (MQ=255)
                                                      tAGATTGTAAGACACCTAGCACAGTTAATACCATATTGGCGGTTAGCTTTGTTTTTGATGATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATACTGCCACTATAGGAATATAGAAATGttacggcgttctcc                                                                                     >  2:239907/1‑129 (MQ=255)
                                                         tttGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTAGATAATGAAATGCCTATAGGTACTGCAACAATGATGGCCGGTAATAATGCCACTGTAGAAATAGAGAAATGTTCCAGTGTCTTCGACa                                                                                  >  1:640159/1‑141 (MQ=255)
                                                         tttGTAAGACACCTGCCACAGTTAATACAATATTGGCAGCTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATATTCCAGTGGTTTCGACa                                                                                  >  2:938169/1‑141 (MQ=255)
                                                          tgttAAGACACCTGCTACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACAt                                                                                 <  2:728902/138‑1 (MQ=255)
                                                             tAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCATTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCaa                                                                              <  2:563815/141‑1 (MQ=255)
                                                                 acacTTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGt                                                                          <  2:134924/141‑1 (MQ=255)
                                                                 acacCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGt                                                                          <  2:377844/141‑1 (MQ=255)
                                                                 acacCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGt                                                                          >  2:675432/1‑141 (MQ=255)
                                                                 acacCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGt                                                                          >  1:922857/1‑141 (MQ=255)
                                                                 acacCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTGTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCAACATCAACTGt                                                                          >  1:755900/1‑141 (MQ=255)
                                                                  cacCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATAAACTGTc                                                                         >  2:480627/1‑141 (MQ=255)
                                                                  cacCTGCCACAGTTAATACAATACTGGCAGAGCGCTTTCTTTTTGACAATAAAATGCCTCGAGGTACTGCACCAATGATCGCAAGTAATAATACCACTATAGAAATAGCAAAATGATCCAGTGTTTTCGACATCAAcaagc                                                                         >  2:406794/1‑137 (MQ=255)
                                                                   cccTGCCAGAGTTAATACAATATTTGCAGTTCGCTGTGTTTTTGATAATAAAAGGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTcc                                                                        <  2:36636/140‑1 (MQ=255)
                                                                   acCTGCCATAGTTAATACAATATTGGCAGTTCGCTTTGTTTCTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTcc                                                                        <  1:480798/141‑1 (MQ=255)
                                                                    ccTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTcca                                                                       >  2:807159/1‑141 (MQ=255)
                                                                      cgCCACATTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTccacc                                                                     <  2:368004/140‑1 (MQ=255)
                                                                         cacaGTTAATACAATTCTGGCAGTTCGCTTTGTTTTTGATAATAACATGCCGATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATg                                                                  <  2:1623/141‑1 (MQ=255)
                                                                         cacaGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTccaccaag                                                                  >  2:109881/1‑139 (MQ=255)
                                                                         cacaGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATg                                                                  >  1:701742/1‑141 (MQ=255)
                                                                         cacaGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCACCCATg                                                                  >  2:149672/1‑141 (MQ=255)
                                                                         cacaGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGACCACCATg                                                                  >  2:1102707/1‑141 (MQ=255)
                                                                         cacaGTTAATACAATATTGCCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATCTTCCAGTGTTTTCGACATCAGCTGTCCACCATg                                                                  >  2:618391/1‑141 (MQ=255)
                                                                            aGTTAATACAATATTGGCATTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATGGAAATGTTCCAGTGTTTTCGACATCAACTGTCCCCCATGTTc                                                               >  2:22114/1‑141 (MQ=255)
                                                                            aGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTc                                                               <  1:441492/141‑1 (MQ=255)
                                                                               tAATACAATATTGGCAGTTCGCTTTGTTTTTTTTTTTAATTTGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTCTTCGACATCAACTGTCCACCCTGTTCATg                                                            >  2:626457/1‑141 (MQ=255)
                                                                               tAATACAATATTGGCAGTTCGCTTTGTTTTTGATAAAAAAATACCAATAGGTACTGCCACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCCCCATGTACagt                                                            >  2:360412/1‑139 (MQ=255)
                                                                                   aCAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAgg                                                        <  1:528133/141‑1 (MQ=255)
                                                                                   aCAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCAACTATCGCAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAgg                                                        >  1:523378/1‑141 (MQ=255)
                                                                                   aCAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCGATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAgg                                                        >  1:584083/1‑141 (MQ=255)
                                                                                      atatTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAAc                                                     >  1:143164/1‑141 (MQ=255)
                                                                                       tatTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACt                                                    <  2:624519/141‑1 (MQ=255)
                                                                                           ggCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAAt                                                >  2:439069/1‑141 (MQ=255)
                                                                                                 tCGCTGTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGAt                                          <  2:226596/141‑1 (MQ=255)
                                                                                                  cGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCCCTATAGAAATCTCGAAATGTTCCAGTGTTTACGACATCAACTGTCCACCATGTTCATTAAGGAACTCAATCATGAtt                                         >  1:38450/1‑141 (MQ=255)
                                                                                                  cGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGAtt                                         >  2:476840/1‑141 (MQ=255)
                                                                                                  cGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCAGATAGGAACTCAATCATGAtt                                         >  1:654138/1‑141 (MQ=255)
                                                                                                   ggtttGTTTTTGATAAGAACGTGCTTATATGTCCTGCAACTATTATGGCAAGTAATGATTCCTCTATAGAAATATAGAAACGTTCCAGTATTTTCGACATCAACTGTCATCCATGTTTATGTAGGAACTCAATCATGAttg                                        <  2:448166/139‑2 (MQ=255)
                                                                                                   gCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGAttt                                        <  2:949976/141‑1 (MQ=255)
                                                                                                   gCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGAttt                                        >  1:130842/1‑141 (MQ=255)
                                                                                                   gCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGAttt                                        <  2:949940/141‑1 (MQ=255)
                                                                                                      ttGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTatc                                     <  1:276452/141‑1 (MQ=255)
                                                                                                      ttGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTatc                                     <  1:958476/141‑1 (MQ=255)
                                                                                                      ttGTTTTTGATAATAAAATGCCTATACGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCGAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTatc                                     <  1:666987/141‑1 (MQ=255)
                                                                                                           tttGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATTATGCCACTATATAGATATAGAAATGTTCCAGTGTTTTCGACATCAACTCTCGACCATGTTCATGTACGAAATCAATCATGAttcttctttta                                >  2:272439/1‑132 (MQ=255)
                                                                                                            ttGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTcc                               <  2:1064804/141‑1 (MQ=255)
                                                                                                             tgttaataaAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCt                              <  2:820470/138‑1 (MQ=255)
                                                                                                             tGATAATAAAATGCCTAGAGGTACTGCAGCAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCATCTGTCCACCTTGTTCATGTAGGAACTCAATCATGATTTATCATCTCCt                              <  2:831652/141‑1 (MQ=255)
                                                                                                                 aataaAATGCCTATAGGTACTGCAACAATGATGGCCCGCAATCCTGCCCCCATCGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGt                          >  1:66119/1‑141 (MQ=255)
                                                                                                                  ataaAATGGCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTGTAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTc                         <  1:475094/141‑1 (MQ=255)
                                                                                                                  ataaAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCGACCATGTGCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTc                         >  2:448763/1‑141 (MQ=255)
                                                                                                                  ataaAATGCATATAGGTACTGCAAAAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGCAATCAATCCAGATTTATCCTCTCCTATGTc                         <  2:646198/141‑1 (MQ=255)
                                                                                                                    aaaaTGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTGAATCATGATTTATCATCTCCTATTTCAc                       >  2:1085914/1‑141 (MQ=255)
                                                                                                                     aaaTGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTAGCATCTCCTATGTCACg                      <  2:212587/141‑1 (MQ=255)
                                                                                                                     aaaTGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCACGTTCATGTAGGAACTCAATCTTGATTTATCATCTCCTATGTCACg                      <  1:807159/141‑1 (MQ=255)
                                                                                                                       aTGTCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTa                    <  1:1080572/141‑1 (MQ=255)
                                                                                                                       aTGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTa                    <  1:107515/141‑1 (MQ=255)
                                                                                                                              taGGTACTTCAACAATGATGGCAAGTAATCATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGAc             <  1:332731/141‑1 (MQ=255)
                                                                                                                              taGGTACTGCAACAATGATGGCAAGTAATAATGTCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTTTCATCTCCTATGTCACGTACTTTGAc             <  1:239358/141‑1 (MQ=255)
                                                                                                                               aGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCGAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGACa            >  2:279548/1‑141 (MQ=255)
                                                                                                                                ggTACTGCAATAATGATGGCAAGTAATAATGCCACTATAGAACTCTCGAAAGCGTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGACAt           <  1:521011/141‑1 (MQ=255)
                                                                                                                                ggTACTGCAATAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGCACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGACAt           <  1:672530/141‑1 (MQ=255)
                                                                                                                                ggTACTGCAATAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGACAt           <  1:513984/141‑1 (MQ=255)
                                                                                                                                 gTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCGTGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGACAtt          <  1:675432/141‑1 (MQ=255)
                                                                                                                                         acaaTGATGGCAAGTAATAATGCCACTGTAGGAATATAGAAATGTTCCAGTGGTTTCGACATCAACTGTCCACAATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTTACGTACTTTGACGTTATTAgtga  >  2:1048562/1‑140 (MQ=255)
                                                                                                                                         acaaTGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGACATTAGTAgtgt  >  2:782346/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TTTTACCAACACCAAAAATCGGTATCATAATAGCAAGTACAGCTAGTGTTGGAATAGTTTGTAAGACACCTGCCACAGTTAATACAATATTGGCAGTTCGCTTTGTTTTTGATAATAAAATGCCTATAGGTACTGCAACAATGATGGCAAGTAATAATGCCACTATAGAAATATAGAAATGTTCCAGTGTTTTCGACATCAACTGTCCACCATGTTCATGTAGGAACTCAATCATGATTTATCATCTCCTATGTCACGTACTTTGACATTAGTAGTGT  >  CP000730/2575822‑2576099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: