BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,087	A→G	59.4%	C155C (TGT→TGC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,087	0	A	G	59.4%	785.2 / inf	1271	C155C (TGT→TGC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base A (261/251); new base G (416/330); total (683/588)
Fisher's exact test for biased strand distribution p-value = 9.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 1272 total aligned reads displayed.

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

  tttATATAGAGCGTAAATTCCCACAACACCGTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACa                                                                                                                                           <  2:771867/141‑1 (MQ=14)

                aaaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGgtttaagtt                                                                                                                             <  1:174491/141‑1 (MQ=255)

                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGgtttaagtt                                                                                                                             >  2:590604/1‑141 (MQ=255)

                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGgtttaagtt                                                                                                                             <  1:1013673/141‑1 (MQ=255)

                 aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGgtttaagttt                                                                                                                            <  2:802131/141‑1 (MQ=255)

                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGTGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGgtttaagttta                                                                                                                           >  1:733432/1‑141 (MQ=255)

                  aTACACTCAATACCTTTTAAAGTATTCTTTGCAGTATTGATCCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCCGatttaagttta                                                                                                                           >  1:124737/1‑141 (MQ=14)

                    accctCAAGACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTaaa                                                                                                                         <  1:363472/137‑1 (MQ=255)

                    acactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACATTGGCAGTCAGGTTTAAGTTTaaa                                                                                                                         >  2:173107/5‑141 (MQ=255)

                    acactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTaaa                                                                                                                         >  1:790084/5‑141 (MQ=255)

                       ctCAATACCTTTTAAAGTATTCTTTGCTGTATTTATACTTTGATACCTTGTATTCACGGCTATAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATGTTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGc                                                                                                                      >  1:479329/2‑141 (MQ=14)

                       ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGc                                                                                                                      >  2:878860/2‑141 (MQ=255)

                        tCAATACCTTTTAAATTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                                                                                                                     <  1:649845/141‑1 (MQ=255)

                        tCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATAGCAGTCAGGTTTAAATTTAAAAGCt                                                                                                                     >  1:408058/1‑141 (MQ=255)

                          aaTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTACTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCttt                                                                                                                   >  1:978798/1‑141 (MQ=255)

                          aaTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTATAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCttt                                                                                                                   >  1:677896/1‑141 (MQ=255)

                            tACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTTAGTTTAAAAGCTTTaa                                                                                                                 >  1:50959/1‑141 (MQ=255)

                              ccTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                                                                                               >  2:934839/1‑141 (MQ=255)

                              ccTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                                                                                               <  1:933469/141‑1 (MQ=255)

                               cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                                                                                              >  2:450889/1‑141 (MQ=255)

                               cTTTTAAAGTATTCTTTGCCGTATTGATACTTTCATATCTTGACTTTCTTAGTTTAATATGACGGGGATGTGGCTCAATGAGGGTATTTAGATATGTAGGTG‑GACAATGACATTCAGGTTTAGGTTTAACAACTTTatatt                                                                                                              >  2:25847/1‑137 (MQ=2)

                                ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                                                                                                             >  2:852298/1‑141 (MQ=255)

                                  ttAACGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTCCCTCACATAGTTCGCTGCTAC‑GTCTCGTTCAGGTGTAAGTTTAAAAGCTTTAATTattt                                                                                                           <  1:809424/141‑4 (MQ=2)

                                   tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                                                                                          <  1:593832/141‑1 (MQ=255)

                                    aaaGTATTCTTTGCTGTATTGATACTTTGATACCTGGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTa                                                                                                         <  1:323176/141‑1 (MQ=255)

                                      aGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACTGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAATCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGc                                                                                                       >  1:343221/1‑141 (MQ=11)

                                           tCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTg                                                                                                  >  1:843449/1‑141 (MQ=255)

                                                      ttGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGtt                                                                                       <  2:43399/141‑1 (MQ=255)

                                                       tGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGATACCTCCGTTg                                                                                      >  2:14475/1‑141 (MQ=255)

                                                         aTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGaa                                                                                    >  2:830418/1‑141 (MQ=255)

                                                          tACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAg                                                                                   >  2:986629/1‑141 (MQ=255)

                                                          tACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAg                                                                                   >  2:781842/1‑141 (MQ=255)

                                                          tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAg                                                                                   <  2:1014073/141‑1 (MQ=40)

                                                           aCTTCGATACTTTGTCTTTCTTACTTTAACATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAACAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAgg                                                                                  <  1:1048868/141‑1 (MQ=14)

                                                             tttGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTg                                                                                <  2:243521/141‑1 (MQ=255)

                                                             tttGATACCTTTTCTTTCTTACTTTAATATGACGGTGATCTTGCTTACTGAGGTCATTCAGTTATTTTGATG‑TACAATGGCAGTCAGGTTTAAGTTTACAAGCTTTAATTACTTTAACCATTGCTACCCTCGTTGAAGGTg                                                                                <  2:277771/141‑1 (MQ=255)

                                                             tttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGTGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTg                                                                                <  2:733432/141‑1 (MQ=255)

                                                               tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAACTTCGTTGTAGGTGcc                                                                              >  2:751949/1‑141 (MQ=255)

                                                                 ttACCGTGTCTTTCTTTCTTTAATATGACGGTGATCTTCCTGAATGAGGTTATTCTGATATTTCGATG‑TACAATGGTAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg                                                                            <  2:412649/140‑1 (MQ=11)

                                                                  tACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa                                                                           >  2:902651/1‑141 (MQ=255)

                                                                   aCCTTGTCTTTCTTACTTTAATATGACGGTGATCGTGCTCAATGAGGTTCTTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAt                                                                          >  1:578161/1‑141 (MQ=255)

                                                                    ccTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATc                                                                         >  1:189882/1‑141 (MQ=255)

                                                                    ccTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATc                                                                         >  2:349768/1‑141 (MQ=255)

                                                                      ttGTCTTTCTTTCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTg                                                                       <  2:921067/141‑1 (MQ=255)

                                                                      ttGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCtt                                                                       >  2:469738/1‑140 (MQ=255)

                                                                      ttGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGATTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTg                                                                       >  2:480803/1‑141 (MQ=255)

                                                                      ttGTCTTTCTTACTTTAATATGACGGAGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCtt                                                                       >  2:1067143/1‑140 (MQ=255)

                                                                          ctttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAt                                                                   >  1:1071941/1‑141 (MQ=255)

                                                                          ctttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGATG‑TGCAATGGCATTCAGGTTTAAGTTTCAAAGCGATAATTACTTTATTCATTGCTCCCTTCGTTGTAGGTGCCTGATCTGTact                                                                   >  1:1085910/1‑139 (MQ=11)

                                                                           tttcttACTTTAATATGACGGTGATGGTGCTCAATGGGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaact                                                                  <  2:676401/141‑3 (MQ=11)

                                                                           tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaact                                                                  <  2:64060/141‑3 (MQ=255)

                                                                              cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                               >  2:230762/1‑141 (MQ=255)

                                                                              cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTAcc                                                               <  1:309929/141‑1 (MQ=255)

                                                                                tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTAAAATTTCTCGCCTGATCTGTAATTACCtt                                                             <  1:264607/141‑1 (MQ=11)

                                                                                 aCTTTAATATGACGGTGATCTTGCACAAGGAGGTTATTCAGATCTTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTCATTACCttt                                                            >  2:68496/1‑141 (MQ=11)

                                                                                  cgtttatatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATCTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATCTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCtttt                                                           <  2:31433/136‑1 (MQ=11)

                                                                                  cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGTTGCCTGTTCTGTAATTACCattt                                                           >  1:224469/1‑141 (MQ=255)

                                                                                   gttAATATTTCGGTGATCTTGCTCAATGTGGCTATTCTGATATTTCGGTG‑TACGATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTg                                                          <  1:891023/140‑1 (MQ=11)

                                                                                     tAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAg                                                        >  1:613087/1‑141 (MQ=25)

                                                                                      aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                       <  2:13056/141‑1 (MQ=21)

                                                                                       atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggc                                                      >  1:1000083/1‑140 (MQ=255)

                                                                                       atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggc                                                      <  2:967784/141‑2 (MQ=255)

                                                                                        tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                     >  1:43269/1‑141 (MQ=35)

                                                                                          tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTCCCTTTTGAGGCTTa                                                   >  2:157265/1‑141 (MQ=11)

                                                                                           gACGGTGTTCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                  <  2:65755/141‑1 (MQ=255)

                                                                                           gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                  >  1:993707/1‑141 (MQ=255)

                                                                                           gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                  >  2:949352/1‑141 (MQ=255)

                                                                                           gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                  <  2:294325/141‑1 (MQ=21)

                                                                                           gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                  >  1:491226/1‑141 (MQ=255)

                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACGATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTATCTATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 >  2:604347/1‑141 (MQ=255)

                                                                                               gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                              >  1:957267/1‑141 (MQ=17)

                                                                                               gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                              >  2:1088096/1‑141 (MQ=11)

                                                                                                  aTCTTGCTCAATGAGGTTATTAGCATTTTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                           >  1:1098461/1‑141 (MQ=11)

                                                                                                   tCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGTTTTACCAGATTGt                                          >  1:914323/1‑141 (MQ=255)

                                                                                                    cTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                         >  2:1039178/1‑141 (MQ=17)

                                                                                                      tGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTACTTACCTTTTGAGGTTTACCAAATTGTTTa                                       >  2:575817/1‑141 (MQ=255)

                                                                                                       gCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                      <  2:705561/141‑1 (MQ=11)

                                                                                                        cTCAATGCGGTTATTCAGATATTTCGATG‑TCCCATGCCAGTCAGGTTTACGCTTAAAAGCTTTACTTCTTTAACCCATTGATGCAATCTGGGTAGGGGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAAt                                     <  1:529578/141‑1 (MQ=2)

                                                                                                        cTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGGCCGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCATATTGTTTAAt                                     >  1:828793/1‑141 (MQ=255)

                                                                                                        cTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAAt                                     <  1:711753/141‑1 (MQ=11)

                                                                                                           aaTGAGGTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                  <  1:242951/141‑1 (MQ=12)

                                                                                                           aaTGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgag                                  <  1:452764/141‑1 (MQ=11)

                                                                                                               gggTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                              <  2:190872/140‑1 (MQ=17)

                                                                                                                 gTTATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCATGCTCAGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                            <  1:758732/141‑1 (MQ=11)

                                                                                                                 gTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAACTTGTTTAATGAGACCttc                            >  2:208324/1‑140 (MQ=11)

                                                                                                                  ttATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGACATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                           >  1:626849/1‑141 (MQ=11)

                                                                                                                  ttATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                           <  2:535113/141‑1 (MQ=32)

                                                                                                                   tATTCAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTGATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                          <  2:386378/141‑1 (MQ=12)

                                                                                                                      tCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                       <  1:209653/141‑1 (MQ=32)

                                                                                                                       cAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGCTaaa                      >  2:1105021/1‑141 (MQ=255)

                                                                                                                       cAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                      >  2:818673/1‑141 (MQ=17)

                                                                                                                       cAGATATTTCGATG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                      >  1:554240/1‑141 (MQ=17)

                                                                                                                       cAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTTTTTAATGAGACGTTTGATacc                      <  2:618857/141‑3 (MQ=17)

                                                                                                                         gATATTTCGATG‑TACAATGGCAGTCAGGTTTACGTTTATAAGATTTAATTACTTTAATCATTGCTACCTTCTTTTAAGGTGCCTTATCTTTAATTACCTTTTGTGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                    <  2:369730/141‑1 (MQ=11)

                                                                                                                          atatTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAGTTGTGAATGAGACGTTTGATAAACGc                   <  2:506521/141‑1 (MQ=11)

                                                                                                                               tCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg              <  2:331992/141‑1 (MQ=255)

                                                                                                                                  aTG‑TACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg           <  1:470214/141‑1 (MQ=17)

                                                                                                                                       aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg       <  1:382138/141‑1 (MQ=11)

                                                                                                                                       aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGCTGTTGCCTGATCTGTAATTACCTTTTGAGGTTTACCACATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg       >  1:150735/1‑141 (MQ=255)

                                                                                                                                         aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt     >  1:869092/1‑141 (MQ=2)

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG‑TACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`