BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,161	T→C	31.8%	I131V (ATT→GTT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,161	0	T	C	31.8%	838.9 / inf	958	I131V (ATT→GTT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (352/296); new base C (120/183); total (475/483)
Fisher's exact test for biased strand distribution p-value = 2.85e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 972 total aligned reads displayed.

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

  ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTGTAAGTTTAAAAGCTTTAATTACTTTCACCATTACTACCTTCGTTGTCAGTGACTGAACTGAG‑ATTa                                                                                                                                            >  2:868985/1‑141 (MQ=14)

  ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTCTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGGGCCTGATCTGTA‑ATTa                                                                                                                                            >  2:824938/1‑141 (MQ=255)

   tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAGGGTGCCTGATCTGTT‑ATTAc                                                                                                                                           >  2:969000/1‑141 (MQ=255)

    cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGGCTTCGTGGAAGGTGCCTGAGCGGTA‑ACTAcc                                                                                                                                          <  2:836961/141‑1 (MQ=11)

    cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTAcc                                                                                                                                          <  1:309929/141‑1 (MQ=255)

     ttACTTTAATGTGACGGTGGTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACGATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCGTCGTTGAAGGTGCCTGATCTGTA‑ACTACCt                                                                                                                                         <  2:164776/141‑1 (MQ=11)

     ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGATGGTGCCTGATCTGTA‑ATTAcgt                                                                                                                                         >  1:985918/1‑139 (MQ=255)

       aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGATGCCTGATCTGTA‑ACTACCttt                                                                                                                                       >  2:133499/1‑141 (MQ=255)

        cgtttatatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATCTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATCTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCtttt                                                                                                                                      <  2:31433/136‑1 (MQ=11)

        cTTTAATTTGACGGTTATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCtttt                                                                                                                                      <  2:429028/141‑1 (MQ=11)

        cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCtttt                                                                                                                                      >  2:463354/1‑141 (MQ=255)

          ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGa                                                                                                                                    <  1:488609/141‑1 (MQ=25)

          ttAATAAGACGGTGATCTTGCCCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGa                                                                                                                                    <  2:239982/141‑1 (MQ=255)

            aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAgg                                                                                                                                  >  1:789518/1‑141 (MQ=255)

             atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAggc                                                                                                                                 >  1:1000083/1‑140 (MQ=255)

               atGACGGTTATCTTTCTCAATGAGGTTCTTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAggctt                                                                                                                               <  1:82830/141‑4 (MQ=11)

               atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTACCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGttt                                                                                                                               <  2:351688/141‑1 (MQ=25)

                tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTa                                                                                                                              >  1:333205/1‑141 (MQ=255)

                tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTa                                                                                                                              <  2:115247/141‑1 (MQ=35)

                 gACGGTGATCTTGCTCAATGCGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTAc                                                                                                                             <  2:288628/141‑1 (MQ=255)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTAc                                                                                                                             >  2:949352/1‑141 (MQ=255)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTAc                                                                                                                             <  1:47853/141‑1 (MQ=11)

                 gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAAGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTAc                                                                                                                             <  1:418277/141‑1 (MQ=11)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTAcc                                                                                                                            >  2:14120/1‑141 (MQ=255)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTAcc                                                                                                                            >  2:67129/1‑141 (MQ=255)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTAcc                                                                                                                            >  2:488101/1‑141 (MQ=255)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTAcc                                                                                                                            >  1:271213/1‑141 (MQ=35)

                     gTGATCTTGCTCACTGGGGCGAATCAGATGTTCCGATGGACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCaaa                                                                                                                         <  1:21884/141‑1 (MQ=11)

                     gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTACCaaa                                                                                                                         <  1:438038/141‑1 (MQ=11)

                        aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGGGGTTTACCAAATTg                                                                                                                      >  1:936793/1‑141 (MQ=255)

                        aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTg                                                                                                                      >  1:762306/1‑141 (MQ=17)

                        aTCTTGCTCAATGAGGTTATTAGCATTTTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTg                                                                                                                      >  1:1098461/1‑141 (MQ=11)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTACAAGCTTTAATTACTTTAACCATTGCTCCCTTCGTTGAAGGTGNCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  2:77769/1‑141 (MQ=255)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  2:585004/1‑141 (MQ=12)

                          cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                    >  1:555480/1‑141 (MQ=17)

                           ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGACACTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGttt                                                                                                                   >  2:602092/1‑141 (MQ=11)

                             tgtCAATGGTGTTATTCAAATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAACTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTACCAAATTGTTTaa                                                                                                                 <  2:1047707/139‑1 (MQ=11)

                             gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTACCAAATTGTTTaa                                                                                                                 <  2:705561/141‑1 (MQ=11)

                             gCTCAAGGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTACGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGACGTTTACCAAATTGTTTaa                                                                                                                 <  2:511481/141‑1 (MQ=11)

                              cgcAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                <  2:740351/139‑1 (MQ=12)

                                cAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                              <  2:7915/141‑1 (MQ=17)

                                cAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                              <  2:154891/141‑1 (MQ=32)

                                 aaTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                             <  2:897954/141‑1 (MQ=12)

                                 aaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTACCAAATTGTTTAATgag                                                                                                             <  1:955235/141‑1 (MQ=11)

                                    gAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACg                                                                                                          <  1:146280/141‑1 (MQ=255)

                                     aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  2:1095414/141‑1 (MQ=17)

                                     aGGTTATTCAGATATTTCGATGTACAAGGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  2:211290/141‑1 (MQ=12)

                                      ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                        <  2:64256/141‑1 (MQ=17)

                                       gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                       >  2:444254/1‑141 (MQ=12)

                                       gTTATTCAGAGATTTGGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                       <  2:201084/141‑1 (MQ=17)

                                        ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGACATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                      >  1:626849/1‑141 (MQ=11)

                                         tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTGATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     <  2:386378/141‑1 (MQ=12)

                                             cAGATATTTTGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                 >  2:1086455/1‑141 (MQ=255)

                                             cAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTTTTTAATGAGACGTTTGATacc                                                                                                 <  2:618857/141‑3 (MQ=17)

                                                  agttCGATGTACAACGGCAGGTAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                            <  2:928736/139‑1 (MQ=11)

                                                     tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                         <  2:362906/141‑1 (MQ=17)

                                                     tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                         <  2:406581/141‑1 (MQ=17)

                                                      tgATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                        <  1:862602/140‑1 (MQ=17)

                                                      cGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTCGAAAAACGCATATGc                                                                                        >  1:1074226/1‑141 (MQ=17)

                                                       gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                       >  1:584934/1‑141 (MQ=255)

                                                       gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTATTTAATGAGACGTTTGATaaagggatgggct                                                                                       >  2:758119/1‑131 (MQ=255)

                                                            aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  >  1:361385/1‑141 (MQ=17)

                                                            aCAATGGCAGTCAGGTTTAAATTTCAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  >  1:99827/1‑141 (MQ=255)

                                                             cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  1:968111/1‑141 (MQ=9)

                                                             cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  2:683220/1‑141 (MQ=9)

                                                             cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 <  1:917450/141‑1 (MQ=17)

                                                             cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  1:517260/1‑141 (MQ=14)

                                                             cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTA‑ACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 <  1:238772/141‑1 (MQ=11)

                                                                                                       accATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAGATATTTAATGTAGGTccc                                       >  2:555319/2‑141 (MQ=12)

                                                                                                       accATTGCCACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGGGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAGCTAATGTATGTccc                                       <  2:254439/140‑1 (MQ=12)

                                                                                                        ccATTGCTACCTTCGTTGAAGATGCCTGATCTCTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcccc                                      >  1:388857/1‑140 (MQ=17)

                                                                                                         cATTGCTAACTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  1:198693/141‑1 (MQ=17)

                                                                                                          aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                    >  1:896560/1‑141 (MQ=32)

                                                                                                           ttGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctctt                                   >  2:532609/1‑140 (MQ=17)

                                                                                                            tgatACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                  <  1:602580/138‑1 (MQ=17)

                                                                                                             gCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                 <  2:98327/141‑3 (MQ=17)

                                                                                                               tACCTTCGTTTAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                               >  1:452823/1‑141 (MQ=17)

                                                                                                                aCCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGcttta                              >  1:682326/1‑137 (MQ=17)

                                                                                                                 atttCGGTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGCTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             <  2:1026084/139‑1 (MQ=255)

                                                                                                                   ttagtcataaatGCCTCATTTGTT‑ATTATCCGTGGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                           <  1:1081135/130‑1 (MQ=11)

                                                                                                                   ttCGTTGAAGGTGCCTGATCGGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                           <  1:1058083/141‑1 (MQ=17)

                                                                                                                    tCGTTGAAGGTGCCTTATCTGTA‑ATTAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATgg                          <  1:650737/141‑1 (MQ=17)

                                                                                                                    tCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAGTATCTAATGTATGTCCCTCTGCATCAATgg                          >  1:159494/1‑141 (MQ=17)

                                                                                                                     cGTTGAAGGTGCCTGATCTGTATATCTACAGTT‑AGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATgga                         >  1:535109/1‑140 (MQ=11)

                                                                                                                      gTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                        >  1:875816/1‑141 (MQ=17)

                                                                                                                        tGAAGGTGCCTGATCTGTA‑ATTACTTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTGCCTCTGCATCAATGGCACg                      >  1:705892/1‑141 (MQ=17)

                                                                                                                          aaGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCTCGat                    >  1:839132/1‑141 (MQ=17)

                                                                                                                           aGGTGCCTGATCTGTA‑ATTACCTTCTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCCCGata                   >  1:806635/1‑141 (MQ=12)

                                                                                                                             gtcccTGATCGGTA‑ACTACCTTTAGAGGCTTACCAAATTGTTAAATTAGACGTTTGTTTAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                 <  2:720022/138‑1 (MQ=11)

                                                                                                                             ggtccTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAtata                 <  2:271213/138‑1 (MQ=17)

                                                                                                                             gTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAtata                 <  2:182408/141‑1 (MQ=32)

                                                                                                                             gTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAtata                 <  1:175152/141‑1 (MQ=32)

                                                                                                                                ccTGATCTGTA‑ATTACCTTATGCCGATTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAt              >  2:1067961/1‑141 (MQ=255)

                                                                                                                                 cttaTCTGTA‑ATTACCTTTTGATGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATa             <  1:124744/138‑1 (MQ=17)

                                                                                                                                  tGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAtgg            >  2:331814/1‑139 (MQ=17)

                                                                                                                                  tGATCTGTA‑ATTACCATTTGAGGTTTACCAAATTGTTTAATGAGACGTTTAATAAACGCATAAGCTGAATGATTATATCGTTGCATAAGCAAGCAAATCTCAAATGTAAGCCCATCTCCATCAATGGCACGATATAAACAg            >  2:227666/1‑141 (MQ=11)

                                                                                                                                   gATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATTTAAATAGc           >  1:724413/1‑141 (MQ=17)

                                                                                                                                      cTGTA‑ATCACCTTTTGAGTTTTACCCAATTCTTACCTGACATGTTCGTTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc        <  1:748728/141‑1 (MQ=11)

                                                                                                                                        gTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAGTATCTAATGTATGTCCCTCTGCATCCATGGCAAGATATACATAGCTCCAt      <  2:813637/141‑1 (MQ=255)

                                                                                                                                         tA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCAtt     >  1:1015850/1‑141 (MQ=17)

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTA‑ATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`