BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	58.2%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	58.2%	265.0 / inf	1159	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (253/228); new base T (291/376); total (549/610)
Fisher's exact test for biased strand distribution p-value = 2.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1171 total aligned reads displayed.

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

 tcTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                             <  2:124051/140‑1 (MQ=17)

    gccTTCTTTGACGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          <  1:646479/140‑1 (MQ=11)

    aCCTTCGTTGAAGGTGCCTGGTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTATTGAGACGTTTGATAAACGCATATGCTGAAAGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          >  1:868230/1‑141 (MQ=255)

    aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACTCTTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGCATCa                                                                                                                                          >  1:107391/1‑141 (MQ=12)

    aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          >  1:766549/1‑141 (MQ=17)

     ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         <  2:1087928/141‑1 (MQ=17)

     atttCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGCTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         <  2:1026084/139‑1 (MQ=255)

       ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGCATGTCCCTCTGTATCAATg                                                                                                                                       <  1:661387/141‑1 (MQ=17)

       ttCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTGATGTATGTCCCTCTGTATCAATg                                                                                                                                       <  2:790689/141‑1 (MQ=11)

       tctgTTTAAGGTGACTGATTTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATg                                                                                                                                       <  1:406653/138‑1 (MQ=255)

           ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTCATGTATGACCCTCTGCATCACTGacac                                                                                                                                   >  2:703045/1‑141 (MQ=255)

           ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAc                                                                                                                                   >  2:203247/1‑141 (MQ=32)

            tGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACg                                                                                                                                  >  1:1048874/1‑141 (MQ=12)

              aaGGTGCTTGATCTGTAACTACCTTTTGAGGCTTCCCAAATTTTTTAATGAGACTTTTGATAAACGCATATGCTGAATTATTATCTCGTTGCTTACGCAAACAAATATCTAATGTATGTCCCTCTGTATCAATGGTACGat                                                                                                                                <  2:343221/141‑1 (MQ=11)

               aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGata                                                                                                                               >  1:422055/1‑141 (MQ=17)

                ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                                                                                                                              >  1:52549/1‑141 (MQ=17)

                 gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                                                                                                                             >  2:989519/1‑141 (MQ=17)

                     ctgaactgaaATAACCTTTTGAGGTATACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATa                                                                                                                         <  1:817979/141‑1 (MQ=255)

                       gATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                                                                                                                       <  2:403021/141‑1 (MQ=12)

                         tCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATCGCTc                                                                                                                     >  2:931923/1‑141 (MQ=255)

                          cTGTGATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACAATATAAATAGCGcc                                                                                                                    >  1:789586/1‑141 (MQ=255)

                          cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                                                                                                                    <  1:703540/141‑1 (MQ=17)

                           tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                   <  1:911394/141‑1 (MQ=17)

                             tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGCCTCAATGGCACGATATCAATAGCTCCAtt                                                                                                                 >  2:460044/1‑141 (MQ=12)

                                   ccTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           <  1:777545/141‑1 (MQ=32)

                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          >  2:842332/1‑141 (MQ=17)

                                       ttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt                                                                                                       >  2:734077/1‑141 (MQ=32)

                                        tGAGGTTTACCAGATTGTGTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                      <  1:12904/141‑1 (MQ=17)

                                        tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                      >  1:224703/1‑141 (MQ=32)

                                         gAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAttt                                                                                                     >  2:519068/1‑141 (MQ=17)

                                            gTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGa                                                                                                  >  1:283508/1‑141 (MQ=17)

                                             tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                 >  2:425659/1‑141 (MQ=17)

                                             tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAAATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                 >  1:948602/1‑141 (MQ=17)

                                             tctACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                 <  1:934839/139‑1 (MQ=17)

                                              ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATg                                                                                                <  2:1101521/141‑1 (MQ=32)

                                                aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                              <  1:309796/141‑1 (MQ=32)

                                                 cccaaTTGTTTAATGCGACGTTTGATAAACGCATATGCTGAATGCTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATAc                                                                                             <  2:828157/138‑1 (MQ=11)

                                                 ccAAATTGTTTAGTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTAc                                                                                             <  1:256518/141‑1 (MQ=17)

                                                  cAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACg                                                                                            <  2:1052976/141‑1 (MQ=12)

                                                        gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                      >  1:835750/1‑141 (MQ=17)

                                                            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                  >  2:560833/1‑141 (MQ=17)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAGGTACGtttctttatta                                                                                 >  1:963001/1‑134 (MQ=255)

                                                              tGAGACGTTTGATAAACGCATTTGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAGTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                <  2:476843/141‑1 (MQ=17)

                                                              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                <  2:768309/141‑1 (MQ=12)

                                                              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                <  1:334613/141‑1 (MQ=32)

                                                                agaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGc                                                                              >  1:167487/1‑141 (MQ=32)

                                                                 gaCGTTTTATAACCGTATATGCTAAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTCTGTCCCTCTGTATCAATGGCACTATATAAATAGCTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                             <  2:362832/141‑1 (MQ=11)

                                                                 gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                             >  2:1001994/1‑141 (MQ=17)

                                                                 gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCCTCAATGGCCCGATATAAATAGCTCCCTTTTCCTTTTTCTTTTATGTTCGTCTGCTCAATACGca                                                                             >  1:1024303/1‑140 (MQ=11)

                                                                  aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAACATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCGTTTATTTTGATATACGTCTCTTCAATACGcct                                                                            >  1:740844/1‑140 (MQ=11)

                                                                   cGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAt                                                                           >  1:457612/1‑141 (MQ=17)

                                                                    gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtt                                                                          <  1:683220/141‑1 (MQ=12)

                                                                     tttGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAttt                                                                         <  2:968740/141‑1 (MQ=12)

                                                                       tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAGCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGt                                                                       >  2:615333/1‑141 (MQ=17)

                                                                        gATAAACGCACATCTTTAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCATTATCCTTTTATTTTGATATACGTCTCCTCACTACGCCATTTGta                                                                      >  2:216481/1‑141 (MQ=11)

                                                                         aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAACTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                     >  1:202426/1‑141 (MQ=255)

                                                                         aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAACAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                     <  2:233567/141‑1 (MQ=255)

                                                                                  tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            <  1:352099/141‑1 (MQ=21)

                                                                                   atGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCCATACGCCATTTGTAATAAGCttttt                                                           >  2:664175/1‑141 (MQ=255)

                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATCAATACGCCATTTGTATTTAACtttttt                                                          >  1:243865/1‑141 (MQ=11)

                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          >  1:1089544/1‑141 (MQ=25)

                                                                                     gCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttttttt                                                         >  2:888621/1‑140 (MQ=25)

                                                                                            gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  >  2:746228/1‑141 (MQ=255)

                                                                                             aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGATTTTTTATGCttttt                                                 >  1:883467/1‑141 (MQ=255)

                                                                                              ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                >  2:630934/1‑141 (MQ=255)

                                                                                              ttATCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                <  1:719676/141‑1 (MQ=255)

                                                                                                agctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              <  2:2941/139‑1 (MQ=255)

                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCCGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCATTACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  2:176696/1‑141 (MQ=255)

                                                                                                 tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  1:893629/141‑1 (MQ=255)

                                                                                                 tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  2:898802/141‑1 (MQ=40)

                                                                                                  ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            <  1:1001560/141‑1 (MQ=255)

                                                                                                   tcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCa                                           <  1:577419/141‑1 (MQ=255)

                                                                                                    cGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaa                                          <  2:145910/141‑1 (MQ=255)

                                                                                                        gcgtaagCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAAttt                                      >  1:612306/7‑141 (MQ=255)

                                                                                                        gCTTACGCAACCAAATATCTAATGTATGTCACTCTGCATCAATGGCACGATATACCTAGCTCAATTTTCCTTTTATTTTGTTGTACTGCCCCTAAGTACGCCGTGTGTATGAATCTTTGTTATACTTTTTCTTCCAAAttt                                      >  2:95412/1‑141 (MQ=2)

                                                                                                         cgtaagCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtttt                                     >  2:717009/6‑140 (MQ=255)

                                                                                                         cTTAGGCAACCAAATATCTAATGTATGTCGCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTg                                     <  2:385729/141‑1 (MQ=255)

                                                                                                         cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTg                                     <  2:867570/141‑1 (MQ=255)

                                                                                                         cTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTGTTATGCTTTTTCTTCCAAATTTg                                     >  2:591542/1‑141 (MQ=37)

                                                                                                             cGCAACCAAATATCTAATTTATGTCCCTCTGCATCAATCGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAc                                 <  2:159494/141‑1 (MQ=255)

                                                                                                              gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATAGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                <  1:984736/141‑3 (MQ=255)

                                                                                                               cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTATTAAGCTTTTTTATGCTGTTTCTTCCACATTTGATATaa                               >  1:440798/1‑141 (MQ=11)

                                                                                                                   cAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTCTTTTCTTCCACATTTCATATaaaact                           >  2:579066/1‑139 (MQ=11)

                                                                                                                   cAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCATTTTTATGCTTTTTCTTCCAATTTTGATATAAAAtt                           >  1:141865/1‑141 (MQ=255)

                                                                                                                    aaagaacTAATGTATTTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                          <  2:807745/135‑1 (MQ=11)

                                                                                                                    aaaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                          >  2:537951/1‑141 (MQ=255)

                                                                                                                          cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCat                    >  2:441653/1‑141 (MQ=255)

                                                                                                                           tAATGTATGTCCCGCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   <  1:184900/141‑1 (MQ=255)

                                                                                                                             atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                 <  1:153044/141‑1 (MQ=255)

                                                                                                                              tgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATATTc                <  1:1109725/141‑1 (MQ=255)

                                                                                                                               ttatgtCTCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt               <  2:107826/140‑1 (MQ=11)

                                                                                                                                tatgtCCCTCTGCATAACTGGCACGATATAAATAGCTCCATTTTCATTTAATTTTTTTGTACTTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt              <  1:84820/141‑1 (MQ=11)

                                                                                                                                   ttCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa           <  2:319185/140‑1 (MQ=18)

                                                                                                                                    tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTGTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc          >  1:595105/1‑141 (MQ=11)

                                                                                                                                    tCCCTCTGTATCAATGGCACGATATAAATAGCTACATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTGGATAGAAAATTGGGGCATATTCTTGAAc          >  1:502922/1‑141 (MQ=11)

                                                                                                                                      ccTCTGTATCAATGGTACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCACTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCGTATTCTTGAAccc        >  1:381323/1‑141 (MQ=11)

                                                                                                                                      ccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCTTATTCTTGAcccc        >  2:257771/1‑141 (MQ=255)

                                                                                                                                        tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTTGGGCATATTTTTGATACCaa      >  1:480575/1‑141 (MQ=11)

                                                                                                                                        tctGCATCAATGGCAAGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTATAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGCTATAAAATAGAGGAATAttgacaaacccaa      >  1:553126/1‑130 (MQ=11)

                                                                                                                                            cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCGATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTAATACAAAATTAGGGCATATTTTTGAACCCAACGGt  >  2:505007/1‑141 (MQ=14)

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`