Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,817 A→T 33.0% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8170AT33.0% 273.8 / 148.8 168intergenic (+48/+60)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (61/51);  new base T (15/40);  total (76/92)
Fisher's exact test for biased strand distribution p-value = 9.68e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

Only 100 of 169 total aligned reads displayed.

AATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35680‑35956
                                                                                                                                         |                                                                                                                                           
aaatattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGt                                                                                                                                          <  1:1078513/138‑1 (MQ=255)
   tattGTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTCTAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCt                                                                                                                                       <  2:801567/141‑1 (MQ=255)
    attCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAGAAAAGCTGACTGAAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTg                                                                                                                                      >  2:1062269/1‑141 (MQ=255)
     ttCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAGTAAAAATAGAAGGGTCGGt                                                                                                                                     >  2:680821/1‑141 (MQ=255)
     ttCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAAGAAAATTGTGATCTAATAAAAATAGAAGGTTCTGt                                                                                                                                     <  1:840586/141‑1 (MQ=255)
       cTGAATTTAAAGATATAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGttc                                                                                                                                   >  2:751064/1‑140 (MQ=255)
          aaTTTAAAGATGTAGATGATCTCATTGATAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCaa                                                                                                                                <  2:973002/141‑1 (MQ=255)
            tttAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAg                                                                                                                              <  2:568990/141‑1 (MQ=255)
            tttAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATACAAGGTTCTGTTGCAAAg                                                                                                                              <  2:521334/141‑1 (MQ=255)
              tAAAGATGTAGATGAACTCATTGACAAAGTATTTAATGTAGACGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGCGCTAAAATAAAATTGTGTTCTAATGAGAATTGGAGGTTCTGTTGCAAAGTa                                                                                                                            >  1:285554/1‑141 (MQ=255)
                 aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                         >  2:550623/1‑141 (MQ=255)
                   aTGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaaaa                                                                                                                       >  1:144127/1‑141 (MQ=255)
                     gTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAat                                                                                                                     <  1:1055394/141‑1 (MQ=255)
                        gatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAGATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAatagg                                                                                                                  >  1:499620/1‑139 (MQ=255)
                        gatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAtata                                                                                                                  >  1:593020/1‑141 (MQ=255)
                                aaTTGACAAAGTCTTTAATGTAGAAGCTATTCACAATAAAGTTCTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATATAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCa                                                                                                          <  2:618297/140‑1 (MQ=39)
                                 aTTGACAAAGAATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTACAATCAACCGCTGCTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                         >  1:802814/1‑141 (MQ=38)
                                        aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTGAAAGAATATAGCTAGCCACTAAtgga                                                                                                  >  2:1110559/1‑138 (MQ=255)
                                        aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                  >  1:553618/1‑141 (MQ=255)
                                        aaGTATTTAAAGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATGTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                  >  1:803203/1‑141 (MQ=255)
                                            aTTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCAt                                                                                              >  2:623484/1‑141 (MQ=255)
                                               tAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCCCTAATTTATAATGTc                                                                                           >  1:1023077/1‑141 (MQ=255)
                                                   ttataaGCTGTTCAGTACAAACGTTTTGGCAAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtg                                                                                       <  2:982855/137‑1 (MQ=37)
                                                   ttAGAAGCTATTCAGGATAAAGTTTTTGGCCAAAAATTATTACTTGAAAAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtg                                                                                       <  2:661798/140‑1 (MQ=255)
                                                             ttCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAAc                                                                             >  1:196600/1‑141 (MQ=255)
                                                               cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                           >  2:937673/1‑141 (MQ=255)
                                                                   ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTACCTTGCTa                                                                       >  2:740068/1‑141 (MQ=255)
                                                                   ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATAATGTAAGTGGTCACACAACTTGATa                                                                       >  2:155972/1‑141 (MQ=37)
                                                                      aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                    >  2:413085/1‑141 (MQ=255)
                                                                      aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                    >  2:568823/1‑141 (MQ=255)
                                                                              ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                            >  1:53124/1‑141 (MQ=255)
                                                                              ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                            >  1:1014073/1‑141 (MQ=255)
                                                                              ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                            >  1:1018136/1‑141 (MQ=255)
                                                                              ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTAGCTTAACTTGCTCGCATGATGCTa                                                            >  1:311092/1‑141 (MQ=255)
                                                                               gCGAAAAATGATTACTTGAACAAGAGATAATTTAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                           >  2:1015712/1‑141 (MQ=255)
                                                                                        gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                  >  2:1102830/1‑141 (MQ=255)
                                                                                        gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                  >  2:1001470/1‑141 (MQ=255)
                                                                                         aTTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCa                                                 >  1:163005/1‑141 (MQ=255)
                                                                                           tACTTGAACCACAGCTAAAATACAATTGTACACCAATAAAAATATAACATTCTGCTGAAAAGTTAAAAAATATAACGTACCACTAATATATCCTGTCAATGTACGCTTAACTTGCTAGCATGATGCTAATTACCTGGCCTg                                               <  2:433676/141‑1 (MQ=9)
                                                                                           tACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATg                                               <  1:422989/141‑1 (MQ=255)
                                                                                                     agatccaaaataagatTGTGATCTAATAAACATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTGACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                     <  2:466239/135‑1 (MQ=17)
                                                                                                     agagCTAAAATAAAATTGGGATCTAATAAAAAGAGAAGGTGCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAGTTATCATGTCAGTGTTCGCTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATcc                                     <  2:602975/141‑1 (MQ=17)
                                                                                                      gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                    <  1:413085/141‑1 (MQ=255)
                                                                                                      gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                    <  1:967827/141‑1 (MQ=255)
                                                                                                              acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                          >  2:766612/15‑141 (MQ=11)
                                                                                                               taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAATATCAGTAGATTTgg                           >  2:771333/1‑140 (MQ=255)
                                                                                                               taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGa                           >  1:694452/1‑141 (MQ=255)
                                                                                                                aaaagagtattttaataaAAATGATGGTGCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        <  2:873985/129‑1 (MQ=11)
                                                                                                                 aaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         >  2:735854/1‑141 (MQ=255)
                                                                                                                  aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTGGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAgaga                      >  2:187880/11‑141 (MQ=11)
                                                                                                                  aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGCAgaga                      >  1:382675/11‑141 (MQ=11)
                                                                                                                  aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                      <  2:314406/131‑1 (MQ=11)
                                                                                                                  aagaggattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCACGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                      <  1:38224/131‑1 (MQ=11)
                                                                                                                   atTGTGTTCTAATTAAAATAGAAGGTTCTGTTGCAAAGTCAAAAAATATAGCTAACCACTAATTTATCATGTCAGCGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                       <  2:853963/141‑1 (MQ=255)
                                                                                                                   agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:467858/132‑1 (MQ=11)
                                                                                                                   agagtattttaaaaaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:357883/132‑1 (MQ=11)
                                                                                                                    ttGTTATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                      <  1:286445/141‑1 (MQ=255)
                                                                                                                    gagtattttaataaGAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  2:811249/133‑1 (MQ=11)
                                                                                                                    gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  1:926845/133‑1 (MQ=11)
                                                                                                                    gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  2:215979/133‑1 (MQ=11)
                                                                                                                    gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  2:927575/133‑1 (MQ=11)
                                                                                                                     tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:1102830/141‑1 (MQ=255)
                                                                                                                     tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:875634/141‑1 (MQ=255)
                                                                                                                     tgggATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:237124/138‑1 (MQ=255)
                                                                                                                     gggtaTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  2:35453/137‑1 (MQ=255)
                                                                                                                     ggggATCGAATGAAAAGAGAAGGTGCTGTTGGAAAGGAAAAAAATAGAGCTGGGCGGGGATTTGGCGTGGCAGTGGGGGCTGATCGTGCTAGCAGGATGCTAAGTTCGTGGCATGGCGAAAATCCGTGGATCTGAagaggc                     <  2:3219/138‑3 (MQ=2)
                                                                                                                     attattttaataaAAATGAGGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACGAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                   <  2:386237/134‑1 (MQ=11)
                                                                                                                      ttcaTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTCCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  1:645543/138‑1 (MQ=255)
                                                                                                                      gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  1:1062269/141‑1 (MQ=255)
                                                                                                                      gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGGGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  2:53124/141‑1 (MQ=255)
                                                                                                                      gtgATCTAATAAAAATAGAAGGGTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  2:169054/141‑1 (MQ=255)
                                                                                                                      gtattttaataaAAATGCTGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATATTGAAAAGCCGTAGAGCTGAAGAGACCTg                  <  2:458433/135‑1 (MQ=11)
                                                                                                                       tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                   <  2:144127/141‑1 (MQ=255)
                                                                                                                       tgATCGAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                   <  2:803203/141‑1 (MQ=255)
                                                                                                                        taTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  1:365912/140‑1 (MQ=255)
                                                                                                                        gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  1:405216/141‑1 (MQ=255)
                                                                                                                        gATCAAATAAAAATAGAAGGTTTTGTTTCAAAGTAAAAAAATAGAGCTAACCACTAATTTAGCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  2:485063/141‑1 (MQ=14)
                                                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCCCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  2:67520/138‑1 (MQ=11)
                                                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  1:452393/138‑1 (MQ=11)
                                                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  1:871186/138‑1 (MQ=11)
                                                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTAAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGAGAAAATCAGTAGATCTGAAGAGAGCTGCgg               >  2:793457/4‑141 (MQ=11)
                                                                                                                         ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCGAATAACTAATATATCATGTAAGTGTTCGTTTAACTTGCTAGCATGATGCTAAATTCGTGGCACGGCGAAAAGCCGTAGATCTGAAGTGAcggtcgg               >  1:379696/4‑135 (MQ=11)
                                                                                                                          tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:71083/139‑1 (MQ=11)
                                                                                                                          tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:132351/139‑1 (MQ=11)
                                                                                                                          tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:126752/139‑1 (MQ=11)
                                                                                                                          tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  1:741772/139‑1 (MQ=11)
                                                                                                                          tcgaataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                <  1:55642/138‑1 (MQ=255)
                                                                                                                           ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             >  2:517987/2‑141 (MQ=11)
                                                                                                                           ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             <  1:437755/140‑1 (MQ=11)
                                                                                                                           cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCAAGTTTCGTGGCATGGCGAGAATCCGTAGCTGTTAAGAGACCTGCgg               >  2:214151/1‑141 (MQ=14)
                                                                                                                            taataaAGAGAGCAGGTTCTGTTGGAAAGTAAAAAAANATAGCTGACCACTAGTTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCCGTAGAGCTGAAGAGACCTGCGGt              <  2:788469/141‑1 (MQ=11)
                                                                                                                            taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc            >  2:1066014/1‑141 (MQ=11)
                                                                                                                              ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAGAATCCGTAGATCTGAAGAGACCTGCGGTTCtt          >  1:341435/1‑141 (MQ=11)
                                                                                                                              ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt          >  1:57121/1‑141 (MQ=11)
                                                                                                                              ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt          >  1:45903/1‑141 (MQ=11)
                                                                                                                               taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGATCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGGGACCTGCGGTTCt           >  2:864337/1‑141 (MQ=255)
                                                                                                                               taaAAATAGAAGGGGCTGTTGCGAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGGCCTGCGGTTCt           >  2:264607/1‑141 (MQ=255)
                                                                                                                                      aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTATTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata    >  1:258374/1‑141 (MQ=17)
                                                                                                                                       gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatat   >  1:618857/1‑141 (MQ=31)
                                                                                                                                        aaGGTACTGTTGCAAAGCAAAAAAATATAGCGAGCCCCTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata  <  2:1027668/141‑1 (MQ=11)
                                                                                                                                         |                                                                                                                                           
AATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35680‑35956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: