BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,444	C→T	48.0%	R36R (AGG→AGA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,444	0	C	T	48.0%	107.0 / inf	437	R36R (AGG→AGA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (82/142); new base T (113/94); total (198/239)
Fisher's exact test for biased strand distribution p-value = 2.26e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

Only 100 of 446 total aligned reads displayed.

TTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTAT  >  CP000730/36304‑36584

tttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc                                                                                                                                              >  2:745572/1‑141 (MQ=25)

 ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                                                             >  1:1000234/1‑141 (MQ=21)

  tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                                                                                            >  2:1064673/1‑141 (MQ=255)

   tttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                           <  2:635237/140‑1 (MQ=18)

   tttgtgattcaCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                           <  2:768763/131‑1 (MQ=11)

   aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                           <  1:943729/141‑1 (MQ=18)

   aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTATTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                           <  2:525485/141‑1 (MQ=16)

   aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCAAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                           <  2:194490/141‑1 (MQ=11)

   aTTTTGATATACGTCTCATCAATACGCCATGTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                           <  2:163536/141‑1 (MQ=11)

    ttttGTTATACGTCTCATCAATACGGCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                                                          <  2:243762/141‑1 (MQ=11)

     tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                                                         <  1:969325/141‑1 (MQ=18)

      ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat                                                                                                                                        <  1:475151/141‑1 (MQ=11)

        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTCTTACAAATATAATACAAAATAGGGGCACATTCTTGAACCAAACGGTAGATCGTGGAATTTTGAACGTTTTCACCACGTTCTCTTAatat                                                                                                                                      >  2:398025/5‑141 (MQ=14)

        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                      >  1:1065558/5‑141 (MQ=11)

        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                      >  1:680811/5‑141 (MQ=255)

        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                                                      >  2:95517/1‑141 (MQ=25)

        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                                                      >  1:528608/1‑141 (MQ=25)

        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCACTTAatat                                                                                                                                      >  1:642254/1‑141 (MQ=21)

        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGCTTACACCACGCTCCCTTAatat                                                                                                                                      >  2:873930/1‑141 (MQ=255)

         atatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                                                                                     >  1:215143/4‑141 (MQ=255)

          tGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                                                                                                    >  1:600797/1‑141 (MQ=21)

          aatACGTCTCAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                                                                                    <  1:401329/139‑1 (MQ=11)

           gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                                                                                   <  1:745001/141‑1 (MQ=21)

            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                                                  <  1:801247/141‑1 (MQ=18)

             aCGTCTCATCAATACNCNATTNGNAATNAGCTTTTTTATGCTTTTTCTTCCCAATTTGATACATAATTGGGGCATATTCTTGTACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                                                                                 <  1:633404/141‑1 (MQ=255)

             aCGTCTCATCAATACGCCATGTGTAATAAGCTTTTTTATGCTTTGTCTTCCAAATTTGCTATAAAATTGGGGCATAGTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTATACACCCCGTACTCTTAATATTTCAg                                                                                                                                 >  1:669706/1‑141 (MQ=11)

              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                                                                                >  1:579699/1‑141 (MQ=25)

              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                                                                                <  1:188214/141‑1 (MQ=21)

              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTGTTCTTCCACATTTGATATAAAATTGGGGAATCTTCATCGACTCATCGGTAGATCGTTGAATCTTTGTCGTTTACACCACGTTTACTTACAATTGCAGa                                                                                                                                <  2:232236/141‑1 (MQ=2)

              cGTCTCATCAATACGCCATCTGTAATAAGCTGTTTTATGCTTTTTCTTTCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                                                                                >  2:279287/1‑141 (MQ=255)

               ttctcATCAATTCGACATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTATATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                               <  1:280822/140‑1 (MQ=11)

               tcctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                                                               <  2:679951/139‑1 (MQ=21)

               gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTAATACCAACGGTAGACTGTTGAATGATGAACGTTTACACCAGGCTCCCTTAATATTTAAGag                                                                                                                               >  2:345385/1‑140 (MQ=11)

               ctcttcgtaaTACGCCAATTGTAATAATCTTGTTTTTGCTTGTTCTTCCAAATTTGATCCAACATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAATCCACGTTCCCTTAATATTTCAGat                                                                                                                               <  2:904421/133‑1 (MQ=11)

                tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                                                                                              <  1:900129/141‑1 (MQ=255)

                tctcAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                                                                                              <  1:173246/141‑1 (MQ=255)

                  tcatcaATACGCCATTTGTAATAAGCTTGTTTATGCTTTTTCTTCCGAATTTGTTTTAAAATTCGGGCATGTTCTTGAACCCAACGGAAGCCCCATGCATGATGACCGTTTATATGACGTTCCCTTAATATCTCAtatata                                                                                                                            >  2:1005148/1‑141 (MQ=2)

                   catcaATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTTGATCTTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatatat                                                                                                                           <  1:983933/141‑1 (MQ=11)

                   catcaATACGCCATTTGTAATAAGCTTTGTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTTAATGATAACCGTTTACAGCACGTTCCCTTAATATTTCAGatatat                                                                                                                           <  1:316696/141‑1 (MQ=11)

                   catcaAAACGCCATTTGTAATAAGCTTTTTTATGCTCTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGATGCACGTTTACACCACGATCTCTTACTATTTCAGttatat                                                                                                                           >  1:377104/1‑141 (MQ=11)

                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTCCACCCCGTTCCCTTCATATTTCAGATATATc                                                                                                                          >  2:727820/1‑141 (MQ=11)

                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                                                         >  2:828332/1‑141 (MQ=255)

                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                                                                                         <  2:1095381/141‑1 (MQ=39)

                      caATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAATTGAGGCATATGTTTGAACCGAATGGTAGATCGTTGAATTATGAACGTTTCCCACACGTTCTCGTACTATTGCAAATATATCAc                                                                                                                        >  2:698721/1‑141 (MQ=2)

                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGTTGAACCCAAGGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTGTTAGTATTTCAGGTATATCAc                                                                                                                        >  2:981068/1‑141 (MQ=11)

                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatatatc                                                                                                                        >  1:844036/1‑138 (MQ=255)

                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCag                                                                                                                        >  1:517141/1‑140 (MQ=255)

                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                                                        <  1:352539/141‑1 (MQ=255)

                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                                                        >  1:816757/1‑141 (MQ=255)

                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                                                        >  1:149732/1‑141 (MQ=255)

                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                                                                                       >  2:911020/1‑141 (MQ=255)

                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCAAGTTCCCTTAATATTTCAGATATATCACg                                                                                                                       <  1:99581/141‑1 (MQ=255)

                       aaTACGACATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCGTATTGTGGAACCCAACGGTAGATCGTTGACTGATGAACGTTTACGCAACGTTCTCTTAATATTTCAGATATATAACg                                                                                                                       >  2:420647/1‑141 (MQ=11)

                        aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                      <  1:272695/141‑1 (MQ=255)

                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACgct                                                                                                                     >  1:408681/1‑139 (MQ=255)

                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                                                     <  2:1036086/141‑1 (MQ=18)

                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                                                     >  1:709359/1‑141 (MQ=25)

                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATGTTCTTGAACCCAACGGTAGACCGTGGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCCGATATATCACGAt                                                                                                                     >  1:158942/1‑141 (MQ=255)

                         tACGCCATCTGTCATAAGCTTCATTATGCATACCCTCCCAAATCTGATATAAAATTTAGGCATAGTCTTTAAGCCAACGAGAGATCGTTGAATGATGAACGTTTACACAACGTCCCCTTAATATCTCAGACAAATCACGaa                                                                                                                     >  2:217912/1‑140 (MQ=2)

                          aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                                                    <  2:865748/141‑1 (MQ=255)

                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATGTCACGATag                                                                                                                   >  1:101943/1‑140 (MQ=11)

                           cGCCATTTCTAATAAGCTTTTTGATGCTTATTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                   <  2:386588/141‑2 (MQ=11)

                            gCCAGTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAAGTGTGATATAAAATTGGGACATATTCTTGAACCCAACGGTAGGCCGTTGCATGATGAACGTTTACAGCACGTTCCCTTAATATTGCTGATAGATCACGATAAc                                                                                                                  <  2:519097/141‑1 (MQ=11)

                            cccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATacc                                                                                                                  <  2:135331/140‑3 (MQ=21)

                              cgtttGTAATAAGCTTTTTCATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                <  2:283508/139‑1 (MQ=255)

                              cagttGTAATACGCTTTTTTATGATTTTTCTTCCAACTTGGATATAAAATTGGGGCATATTCGTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGCTATATCACGATAGCTc                                                                                                                <  1:7660/138‑1 (MQ=11)

                              cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                <  2:554362/141‑1 (MQ=11)

                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAGATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAGACCACGTTCTCTTAATATTTCCGATCTATCACGATAGCTCaa                                                                                                              >  1:952977/1‑141 (MQ=11)

                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCat                                                                                                              >  1:795357/1‑140 (MQ=21)

                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                              <  1:1080571/141‑1 (MQ=21)

                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                              <  1:331814/141‑1 (MQ=18)

                                 ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTCATATTTCAGAGATATCACGATAGCTCAAt                                                                                                             <  1:535938/141‑1 (MQ=11)

                                 ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCCCGATAACTCAAt                                                                                                             >  1:456061/1‑141 (MQ=25)

                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                            <  2:118648/141‑1 (MQ=255)

                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                            <  1:1045434/141‑1 (MQ=25)

                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                            >  1:924591/1‑141 (MQ=21)

                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                            <  1:134728/141‑1 (MQ=21)

                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTTGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                            <  2:334728/141‑1 (MQ=255)

                                   gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                           >  1:396809/1‑140 (MQ=18)

                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCGCGATAGCTCAATgca                                                                                                          <  1:555319/141‑3 (MQ=11)

                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                          <  1:832353/141‑3 (MQ=11)

                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                          >  1:1091387/1‑139 (MQ=255)

                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                          <  1:438938/141‑3 (MQ=11)

                                     aataaGCTTTTTTATGCTTTTGCTGCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTaaaagcat                                                                                                         >  2:614383/1‑136 (MQ=11)

                                         aGCGTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCt                                                                                                     <  2:595163/141‑1 (MQ=37)

                                          gCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCtt                                                                                                    <  2:301953/141‑1 (MQ=37)

                                            ttttttATGCTTTTTCTTCCAAATTTGATACAAACTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCttga                                                                                                  >  2:933238/1‑139 (MQ=25)

                                                    gCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGc                                                                                          <  1:423579/141‑1 (MQ=21)

                                                       ttttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCaa                                                                                       <  2:844571/141‑1 (MQ=35)

                                                           cttcCAAATTTGATACAAAATTGGGGCATATTCTGGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc                                                                                   <  1:383293/141‑1 (MQ=25)

                                                             tcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa                                                                                 <  2:1020833/141‑1 (MQ=34)

                                                                       aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTCCAGTGATaaa                                                                       >  2:481456/1‑140 (MQ=25)

                                                                          caaaaatcaggCATATTCCTGAACCCAACCGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATCTCAAATATATCACGATAACTCAATTTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATc                                                                    <  1:233011/132‑1 (MQ=11)

                                                                           aaaaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATcc                                                                   <  2:78107/141‑1 (MQ=32)

                                                                                    gCATATTCTTGAACCCAACGGTAGACCGGTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATACCTTAGATAGTAGCCAACGGCTCCAGTGATAACATCCTTGTTaaaa                                                          >  2:892995/1‑140 (MQ=12)

                                                                                               aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                               <  1:730707/141‑1 (MQ=32)

                                                                                                   cAACGGTAGACCGTTGAATGATGAATGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGgaa                                           <  2:459244/141‑1 (MQ=17)

                                                                                                              cGTTGAATGATGAACGTTTACCCCTCGTTCCCTTAATATTTCAGATATAACACCACACGGCAGCGTCTATCTCACATAGTAGCCCTGGGTCACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACa                                <  2:501892/141‑1 (MQ=2)

                                                                                                                ttGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACaga                              <  2:536629/141‑1 (MQ=32)

                                                                                                                     tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACt                         <  2:793340/141‑1 (MQ=32)

TTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTAT  >  CP000730/36304‑36584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`