Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,471 A→G 23.8% S27S (AGT→AGC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4710AG23.8% 289.0 / 97.0 187S27S (AGT→AGCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (52/88);  new base G (22/22);  total (75/112)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

Only 100 of 190 total aligned reads displayed.

CGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACC  >  CP000730/36331‑36607
                                                                                                                                            |                                                                                                                                        
 gCCAGTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAAGTGTGATATAAAATTGGGACATATTCTTGAACCCAACGGTAGGCCGTTGCATGATGAACGTTTACAGCACGTTCCCTTAATATTGCTGATAGATCACGATAAc                                                                                                                                         <  2:519097/141‑1 (MQ=11)
  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGGTAGCt                                                                                                                                        >  1:1059699/1‑141 (MQ=11)
  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAAACCACGTTCTCTTAATATTTCAGATATATCACGATAACt                                                                                                                                        >  1:272482/1‑141 (MQ=255)
  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGGTGGACGTTTACACCACGTTCCCTTAATATTTCGGATATATCAGGATAACt                                                                                                                                        >  2:547938/1‑141 (MQ=255)
  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGATCCCTTAATATTTCAGATATATCACGATAAcc                                                                                                                                        >  2:905993/1‑140 (MQ=255)
   cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTATACCGGTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                       <  2:119730/141‑1 (MQ=255)
   cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                       <  2:554362/141‑1 (MQ=11)
    aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                                                      <  1:144128/141‑1 (MQ=18)
     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGAAATATCACGATAGCTCaa                                                                                                                                     >  1:447334/1‑141 (MQ=11)
     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                                                     >  1:1110001/1‑141 (MQ=21)
     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCATTACGAGTGCAGATATATCACGATAACTCaa                                                                                                                                     <  2:506909/141‑1 (MQ=11)
     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGAAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATCTTTACGATATATCCCGATAACTCaa                                                                                                                                     >  1:34876/1‑141 (MQ=11)
     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAGTTGATATAACATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                                                     <  1:279139/141‑1 (MQ=255)
     tttGTAATAAGCTTTTTTATGATTTTTCTTCCCAATTTGATATAGAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                     <  2:941008/141‑1 (MQ=11)
      ttGTAATCAGCTTTTTTACTCTTTATCTTCCAAATTTGTTACTATATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                    <  1:640565/141‑1 (MQ=11)
      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                    >  1:213008/1‑141 (MQ=11)
      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTCATATTTCAGAGATATCACGATAGCTCAAt                                                                                                                                    <  1:535938/141‑1 (MQ=11)
      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                    <  2:736337/141‑1 (MQ=11)
      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                    <  2:767042/141‑1 (MQ=255)
      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                    >  1:82171/1‑141 (MQ=25)
      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                    <  1:734077/141‑1 (MQ=21)
       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATATCTCAATg                                                                                                                                   >  2:22202/1‑141 (MQ=255)
       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                   <  2:552168/141‑1 (MQ=11)
       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                   <  2:777544/141‑1 (MQ=255)
       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                   >  1:926216/1‑141 (MQ=11)
       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                   <  1:134728/141‑1 (MQ=21)
       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTTGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                   <  2:334728/141‑1 (MQ=255)
        gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATAACGATAGCTCAATgc                                                                                                                                  >  2:643073/1‑140 (MQ=11)
        gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                  >  1:688386/1‑140 (MQ=17)
        gTAATAAGCTTTTTTATGCTTTTTCTGCTAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                  >  2:784012/1‑140 (MQ=255)
        gTAATAAGCTTTATTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACTCAACGGTAGACCGTTGAATGATGAACGTTTAAACCACGGTCCCTTAATATTTCAGATACATCAGGATAACGCAATgc                                                                                                                                  >  2:602046/1‑140 (MQ=11)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCATGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                 >  1:579541/1‑139 (MQ=11)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCGCGATAGCTCAATgca                                                                                                                                 <  1:555319/141‑3 (MQ=11)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                 <  1:832353/141‑3 (MQ=11)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                 <  1:979692/141‑3 (MQ=11)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                 <  1:997953/141‑3 (MQ=11)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                 >  1:464725/1‑139 (MQ=11)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                 >  1:4306/1‑139 (MQ=11)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                                                 >  2:781960/1‑139 (MQ=17)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                                                 <  1:188116/141‑3 (MQ=17)
         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTCGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                 <  2:858257/141‑3 (MQ=11)
         taataaGCTTTTTTATGCTTTATCTCCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                                                 >  2:1068018/1‑139 (MQ=255)
             aaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCCGATATATCACGATAACTCAATGtatctt                                                                                                                             >  2:70004/1‑138 (MQ=255)
              aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCt                                                                                                                            <  2:842324/141‑1 (MQ=38)
              aGCGTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCt                                                                                                                            <  2:595163/141‑1 (MQ=37)
               gCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCtt                                                                                                                           <  1:758680/141‑1 (MQ=38)
               gCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCtt                                                                                                                           <  2:110127/141‑1 (MQ=38)
               gCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCtt                                                                                                                           <  2:301953/141‑1 (MQ=37)
                 ttttttATGCTTTTTCTTCCAAATTTGATACAAACTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCttga                                                                                                                         >  2:933238/1‑139 (MQ=25)
                     tttttttttttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTTCCTTATTATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtaa                                                                                                                     >  2:149320/7‑140 (MQ=255)
                     ttATGCTTTTTCTTTCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                                                                                                     <  2:281706/141‑1 (MQ=25)
                     ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATTTTAGAtag                                                                                                                     >  1:161980/1‑141 (MQ=21)
                     ttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                                                                                                     >  1:347523/1‑141 (MQ=35)
                      tATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATGCCGATAACTCAATGTATATCTTAGAtagt                                                                                                                    >  2:527862/1‑141 (MQ=21)
                       aTGCTTCTTCTTCCAACTTTGATACAAAATTGGGGCACATTCTTGCACCCAAAGGTTGATCGTTGAATGTTGTACGTTTCCACGCCATTCTCTTATTATTCTCGATATATTACGATCGATCAATGTCTCTCCTAGAtagta                                                                                                                   >  2:1029873/1‑141 (MQ=255)
                        tGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGACAACTCACTGTATATCTTAGAtagtag                                                                                                                  >  1:380345/1‑141 (MQ=21)
                         gCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGc                                                                                                                 <  1:423579/141‑1 (MQ=21)
                         gCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGc                                                                                                                 <  1:509463/141‑1 (MQ=35)
                                cttcCAAATTTGATACAAAATTGGGGCATATTCTGGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc                                                                                                          <  1:383293/141‑1 (MQ=25)
                                  tcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa                                                                                                        <  2:1020833/141‑1 (MQ=34)
                                      aaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGt                                                                                                    <  1:795709/141‑1 (MQ=35)
                                      aaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGt                                                                                                    <  2:900364/141‑1 (MQ=35)
                                            aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTCCAGTGATaaa                                                                                              >  2:481456/1‑140 (MQ=25)
                                               caaaaatcaggCATATTCCTGAACCCAACCGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATCTCAAATATATCACGATAACTCAATTTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATc                                                                                           <  1:233011/132‑1 (MQ=11)
                                                   aTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATACATCCTTGt                                                                                      <  1:325712/141‑1 (MQ=17)
                                                    ttGAGGCATTTTCTTGCACCCAACCGTAGATCGATGGATGATGTCATTCCACACCACGGTCACTTAATATTTCAGATAGATCACGATTGCTAAGTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                      <  2:387604/141‑1 (MQ=2)
                                                    tgggggTATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                      <  1:838133/139‑1 (MQ=17)
                                                         gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                                                 >  1:328832/1‑141 (MQ=32)
                                                         gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                                                 >  1:406478/1‑141 (MQ=32)
                                                         gCATATTCTTGAACCCAACGGTAGACCGGTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATACCTTAGATAGTAGCCAACGGCTCCAGTGATAACATCCTTGTTaaaa                                                                                 >  2:892995/1‑140 (MQ=12)
                                                              ttCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACCGTGATAACATCCTTGTTAAATTGttt                                                                            >  1:62142/1‑141 (MQ=17)
                                                                    aaCCCAACGGTCACCCATTGAATGCTGAACGCTTACAGCCCGTTCCGTTTCGAATTCAGATATATCACGATCACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                      <  1:1031824/141‑1 (MQ=11)
                                                                    aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATAGCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                      <  2:291439/141‑1 (MQ=17)
                                                                     aCCCAACGGTAGATAGTTGTATGATCAACGTTTACACCACGTTCTATTAATATTTCAGATATATCACAATAGATCAATGTATATCTTAGTTTGTAGCTAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTTTATCTg                                                                     <  2:752/141‑1 (MQ=11)
                                                                     aCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCATTATATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTg                                                                     >  1:1088459/1‑141 (MQ=12)
                                                                      cccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa                                                                    >  1:1030341/1‑141 (MQ=32)
                                                                        cAACGGTAGACCGTTGAATGATGAATGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGgaa                                                                  <  2:459244/141‑1 (MQ=17)
                                                                        cAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTACATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                  <  1:793892/141‑1 (MQ=17)
                                                                        cAACGGTAGACCAGTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAACCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                  <  1:811360/141‑1 (MQ=12)
                                                                             gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTACATTGTTGATATCTGAAATAGtt                                                             >  1:760133/1‑141 (MQ=17)
                                                                             gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                             >  2:331239/1‑141 (MQ=32)
                                                                                                  gTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTg                                        <  1:39121/141‑1 (MQ=32)
                                                                                                   tttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGt                                       <  1:181807/141‑1 (MQ=32)
                                                                                                    ttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                      <  1:933500/141‑1 (MQ=32)
                                                                                                    taacacCACGTTCCCTTAATAGTTCAGATATATCACGATAACCCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGCCTCCTTTTTGtt                                      <  2:158942/139‑1 (MQ=255)
                                                                                                           acGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAttt                               >  1:488495/1‑140 (MQ=17)
                                                                                                                 ccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACtat                         <  1:727868/141‑1 (MQ=32)
                                                                                                                      atatTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAtt                    <  2:230664/141‑1 (MQ=35)
                                                                                                                       tatTTCATATATATCACGATAACTCAATGTTTATACTAGATAGTACTCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTc                   <  1:573372/141‑1 (MQ=11)
                                                                                                                          ttCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTGCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAAc                >  1:691350/1‑141 (MQ=25)
                                                                                                                            cAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACtt              >  1:285069/1‑141 (MQ=35)
                                                                                                                                  tatCACTATAACGCAATGGATATGTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCCTACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc        <  2:428043/141‑1 (MQ=11)
                                                                                                                                  tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc        >  1:659772/1‑141 (MQ=35)
                                                                                                                                  tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTAAACTTTGCAAc        >  1:1046244/1‑141 (MQ=25)
                                                                                                                                    tCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAg      >  1:314302/1‑141 (MQ=35)
                                                                                                                                       cGATTACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAc   <  1:905993/141‑1 (MQ=25)
                                                                                                                                       cGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAc   <  1:594818/141‑1 (MQ=35)
                                                                                                                                        gATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGt                                       >  1:1101671/1‑104 (MQ=32)
                                                                                                                                        gATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGt                                       <  2:1101671/104‑1 (MQ=33)
                                                                                                                                        caTAACTCAACGGATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGATGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACTGTAcc  <  2:837565/140‑1 (MQ=11)
                                                                                                                                            |                                                                                                                                        
CGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACC  >  CP000730/36331‑36607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: