Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,574,491 A→T 4.5% L1009H (CTC→CAC)  USA300HOU_1441 ← possible bacteriophage tail protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,574,4910AT 4.5% 296.3 / 2.5 102L1009H (CTC→CAC) USA300HOU_1441possible bacteriophage tail protein
Reads supporting (aligned to +/- strand):  ref base A (45/51);  new base T (2/3);  total (47/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GCTCTTTTTCAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCTTTTTTCT  >  CP000730/1574351‑1574631
                                                                                                                                             |                                                                                                                                            
gctcttTTTCTGTCTTACTCACTTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAGATATGACCATCACTCAACGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGa                                                                                                                                              >  2:256405/1‑141 (MQ=255)
gctcttTTTCGGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGa                                                                                                                                              >  1:801985/1‑141 (MQ=255)
gctcttTTTCAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCATTTCTTTGATTTTCTGATTGa                                                                                                                                              >  1:736792/1‑141 (MQ=255)
gctcttTTTCAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGa                                                                                                                                              >  2:510240/1‑141 (MQ=255)
gctcttTTTCAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGa                                                                                                                                              >  1:689712/1‑141 (MQ=255)
 ctctctTTCAGTCTTACTCTATTCTTTAACAGTGCTATCACGTCTTCGATTTTCAAGCTTTTCAATTTCTTTTCTCTCATTTTCTGAAATCTGTCCATCACTCAAAGGTCTTTCTTTCACTTCTTTGATTTTCTGATTGAg                                                                                                                                             <  2:835369/141‑1 (MQ=255)
  tgttgttCAGTCTTACTCCGTGCTTTAACCGTGATCTCACGTCTTTGATTTTCAAGCTTTTTAATTTTTTTTCTGTCATTTTCTGCTATCTGCCCAGCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGt                                                                                                                                            <  2:422297/136‑1 (MQ=255)
  tcttTTTCAGTCTTACTCAATTCTTTAACATTGATATCACGTCTGTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTGAAAGCTTTTTCGTTCAATTCTTTGATTTTCTGATTGAGt                                                                                                                                            <  2:41928/141‑1 (MQ=255)
     ttttCATTCTTACTTAATTCTTTAACCGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCt                                                                                                                                         <  2:479326/141‑1 (MQ=255)
     ttttCATTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCCACGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCt                                                                                                                                         <  2:1010948/141‑1 (MQ=255)
     ttttCAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGTGTTCt                                                                                                                                         >  2:1007424/1‑141 (MQ=255)
     ttttCAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGTGTTCt                                                                                                                                         >  2:810718/1‑141 (MQ=255)
     ttttCAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATCTTCTGATTGAGTTCt                                                                                                                                         <  1:822429/141‑1 (MQ=255)
         cAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGct                                                                                                                                     >  2:594560/1‑141 (MQ=255)
         cAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGct                                                                                                                                     >  1:572729/1‑141 (MQ=255)
             cTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCttt                                                                                                                                 <  1:243179/141‑1 (MQ=255)
              ttACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGTCCTACACCCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTAATTGAGTTCTTGCTCtttt                                                                                                                                >  1:525920/1‑141 (MQ=255)
              ttACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCtttt                                                                                                                                >  1:1053260/1‑141 (MQ=255)
              ttACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCtttt                                                                                                                                >  1:744018/1‑141 (MQ=255)
                      ttCTTTTACAGTCATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTTTTTCATTTTCTGAAATCTGACCATCACTCCAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCg                                                                                                                        <  1:661499/141‑1 (MQ=255)
                      ttCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCg                                                                                                                        <  1:1110110/141‑1 (MQ=255)
                          ttAACAGTGATATCACGTCTTTGTTTTTCAAGCTTTTCAATTTCTTTTCTTTCTTTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAg                                                                                                                    <  1:802510/141‑1 (MQ=255)
                           tAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAGGt                                                                                                                   >  1:537711/1‑141 (MQ=255)
                           tAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGt                                                                                                                   >  1:755588/1‑141 (MQ=255)
                             aCAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCa                                                                                                                 >  2:519579/1‑141 (MQ=255)
                              cAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCAt                                                                                                                <  1:355398/141‑1 (MQ=255)
                                gcgATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGTTTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCAttt                                                                                                              >  2:817237/3‑141 (MQ=255)
                                gTGATATCACGTCTTTGATCTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTTTTTCTATTTTTTGATTTTCTGATTTAGTTCTTGCTCTTTTTTAATACGCAAGTCAttt                                                                                                              >  2:618931/1‑141 (MQ=255)
                                 tGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCAtttt                                                                                                             >  2:902865/1‑141 (MQ=255)
                                 tGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCAtttt                                                                                                             >  2:34508/1‑141 (MQ=255)
                                  gATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCAttttt                                                                                                            <  1:131213/141‑1 (MQ=255)
                                     atCACGTCTTTGATTTACAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCAtttttttt                                                                                                         >  1:566706/1‑140 (MQ=255)
                                       cACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTGGCAAGTCATTTTTTTCtt                                                                                                       >  2:443782/1‑141 (MQ=255)
                                        aCGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCttt                                                                                                      >  1:872747/1‑141 (MQ=255)
                                        aCGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTATTTCATTTTCTGCCATCTGTACATCACTCAAAGCTTTTTCTTTCAATTCCTTGATTTTCTGATTGAGTTCTTGCGCTTTTTTAATTCGCAAGTCATTTTTTTCttt                                                                                                      >  2:641147/1‑141 (MQ=255)
                                                  ttttCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGtt                                                                                            <  1:442083/141‑1 (MQ=255)
                                                  ttttCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGtt                                                                                            <  1:454484/141‑1 (MQ=255)
                                                  ttttCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGtt                                                                                            >  1:762977/1‑141 (MQ=255)
                                                  ttttCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTAAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGtt                                                                                            <  1:521698/141‑1 (MQ=255)
                                                        aGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAATATg                                                                                      >  2:300758/1‑141 (MQ=255)
                                                        aGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATg                                                                                      >  1:838627/1‑141 (MQ=255)
                                                        aGCTTTTCAATTTCGTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATg                                                                                      >  2:314304/1‑141 (MQ=255)
                                                          cTTTTCAATTTTTTTTCTTTCGTTTTCTGATATCTGACCTTCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGtt                                                                                    <  2:951032/141‑1 (MQ=255)
                                                               cAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCTATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGGGTTAAGATGTTTTGCt                                                                               >  2:442393/1‑141 (MQ=255)
                                                               cAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCt                                                                               >  2:804728/1‑141 (MQ=255)
                                                               cAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCt                                                                               >  2:1083156/1‑141 (MQ=255)
                                                                aaTTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCtt                                                                              <  2:416711/141‑1 (MQ=255)
                                                                  tttcttttctTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCACGTCATTTTTTTCTTTAGTTCGCGTTAAAATGTTTTGCtttt                                                                            >  1:129246/1‑141 (MQ=255)
                                                                  tttcttttctTTCATTTTCTAAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTCAGTTCGAGTTAAAATGTTTTGCtttt                                                                            >  1:668374/1‑141 (MQ=255)
                                                                    tcttttctttCATTTTCTGAAATCTGACCATCACTCAACGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCt                                                                          <  2:853169/141‑1 (MQ=255)
                                                                    tcttttctttCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCTATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCt                                                                          >  1:862574/1‑141 (MQ=255)
                                                                       tttctttcTTTTTCTTGAATCTGGCTATCGCTCGAAGCTTTTTCTTTCAATTCTTTGTTTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGt                                                                       <  2:660434/141‑1 (MQ=255)
                                                                       tttctttcATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGt                                                                       <  2:811942/141‑1 (MQ=255)
                                                                           tttcATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCCATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTGTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCAt                                                                   <  1:630643/141‑1 (MQ=255)
                                                                           tttcATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCAt                                                                   <  2:592772/141‑1 (MQ=255)
                                                                            ttcCTTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTACTTCGAGTTCAAATGTTTTGCTTTTCTTGTTCATc                                                                  <  2:398931/141‑1 (MQ=255)
                                                                            ttcATTTTCTGATATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGCGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATc                                                                  <  2:798460/141‑1 (MQ=255)
                                                                            ttcATTTTCTGAAATCTTACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATc                                                                  <  2:1372/141‑1 (MQ=255)
                                                                            ttcATTTTCTGAAATCTGATCATCTCTCAAAGCTTTTTCTTTTAATTCTTTGTTTTTCTGATTGTGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATTTTTTGCTTTTCTTGTTCATc                                                                  <  1:1055006/141‑1 (MQ=255)
                                                                            ttcATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATc                                                                  <  2:1053478/141‑1 (MQ=255)
                                                                            ttcATTTTCTGAAATCTGACCATCACTCAAAGCTTTGTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTGTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATc                                                                  <  1:289443/141‑1 (MQ=255)
                                                                                   tCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCACGTCATTTTTTTCTTTTGTTAGAGTTAAAATGTTTTACTTTTCTTTTTCAtttaaggc                                                            >  2:759236/1‑134 (MQ=255)
                                                                                     tGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTACCTGGAGGTCGAGTTAAAATGTTGTGCTTTTCTTGTTCATCGAATGCACt                                                         >  1:1083191/1‑141 (MQ=255)
                                                                                       aaaTCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGTGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGGATGCACtat                                                       <  1:810718/141‑1 (MQ=255)
                                                                                       aaaTCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGTGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACtat                                                       <  1:1007424/141‑1 (MQ=255)
                                                                                         aTCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTATGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGACTGCACtatcc                                                     >  1:36705/1‑139 (MQ=255)
                                                                                             gACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTAt                                                 >  1:976035/1‑141 (MQ=255)
                                                                                              aCCATCCCTCAACGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATc                                                <  1:349853/141‑1 (MQ=255)
                                                                                               ccATCTCTTCAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCa                                               <  2:92188/141‑1 (MQ=255)
                                                                                               ccATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCa                                               >  1:989405/1‑141 (MQ=255)
                                                                                                    aCTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAg                                          >  1:630409/1‑141 (MQ=255)
                                                                                                       cAAAGCTTTTTTTTTTTTTTTTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTCAAATGTTTTGCTTTTCTTGTTCTTCGAATGCTTTTTTCTTATCAATAAGttt                                       >  2:157307/1‑140 (MQ=255)
                                                                                                       cAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATCCTTATCAATAAGttt                                       >  2:250642/1‑140 (MQ=255)
                                                                                                           gCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCCATAAGTTCTTgg                                   >  2:219881/1‑140 (MQ=255)
                                                                                                           gCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATCATTTCTTGa                                   >  2:261663/1‑141 (MQ=255)
                                                                                                            cTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAg                                  <  2:360220/141‑1 (MQ=255)
                                                                                                                 tctttcAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGttttt                             <  1:866275/141‑1 (MQ=255)
                                                                                                                      tcAATTCTTTTGTTTTCTGATTTAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAATATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGa                         <  2:626344/141‑1 (MQ=255)
                                                                                                                       ccaTTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAg                        <  1:409664/139‑1 (MQ=255)
                                                                                                                       cAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCggg                        >  1:150667/1‑139 (MQ=255)
                                                                                                                       cAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAg                        >  1:664755/1‑141 (MQ=255)
                                                                                                                            cTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCt                   <  1:594560/141‑1 (MQ=255)
                                                                                                                             tttGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCtt                  <  2:264880/141‑1 (MQ=255)
                                                                                                                             tttGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCtt                  <  1:45156/141‑1 (MQ=255)
                                                                                                                               tcaTTTTCTGGTTGAGTTTTTGGTTTTTTTTAATTCGCAGGTCATTTTCTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCCTCGAATGCACTATACTTTTCAATAAGTTCTTGAGTTTTTTCGAGTTCCtttt                <  1:1029461/139‑1 (MQ=255)
                                                                                                                               tGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCtttt                <  2:761687/141‑1 (MQ=255)
                                                                                                                                  ttttttGATTGAGTTCTTGCTCTCTTTTCATTCGCAAGTCCTTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTAt             <  1:256405/141‑1 (MQ=255)
                                                                                                                                  ttttCTGATTGAGTTTTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTAt             <  2:689712/141‑1 (MQ=255)
                                                                                                                                  ttttCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTAt             <  2:736792/141‑1 (MQ=255)
                                                                                                                                  ttttCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTAt             <  2:328577/141‑1 (MQ=255)
                                                                                                                                   tttCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTAtt            <  1:953910/141‑1 (MQ=255)
                                                                                                                                   tttCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTAtt            <  2:14391/141‑1 (MQ=255)
                                                                                                                                     tCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTc          <  1:134435/141‑1 (MQ=255)
                                                                                                                                      cTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCt         >  1:488454/1‑141 (MQ=255)
                                                                                                                                          ttGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCttttt     <  1:1083156/141‑1 (MQ=255)
                                                                                                                                           tGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCtttttt    >  1:619122/1‑141 (MQ=255)
                                                                                                                                            gAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCTTTTTTc   <  1:1013881/141‑1 (MQ=255)
                                                                                                                                            gAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCTTTTTTc   <  2:340105/141‑1 (MQ=255)
                                                                                                                                             aGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCTTTTTTCt  <  1:902865/141‑1 (MQ=255)
                                                                                                                                             aGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCTTTTTTCt  <  1:34508/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GCTCTTTTTCAGTCTTACTCAATTCTTTAACAGTGATATCACGTCTTTGATTTTCAAGCTTTTCAATTTCTTTTCTTTCATTTTCTGAAATCTGACCATCACTCAAAGCTTTTTCTTTCAATTCTTTGATTTTCTGATTGAGTTCTTGCTCTTTTTTAATTCGCAAGTCATTTTTTTCTTTAGTTCGAGTTAAAATGTTTTGCTTTTCTTGTTCATCGAATGCACTATACTTATCAATAAGTTCTTGAGTTTTTTCGAGTTCCTTTTTATTTCTTTTTTCT  >  CP000730/1574351‑1574631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: