Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,837,421 T→C 4.8% E259G (GAA→GGA)  USA300HOU_1714 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,837,4210TC 4.8% 279.1 / 2.7 99E259G (GAA→GGA) USA300HOU_1714hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (63/30);  new base C (3/2);  total (67/32)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

ATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTGGTCTCAAAA  >  CP000730/1837282‑1837559
                                                                                                                                           |                                                                                                                                          
atatGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTc                                                                                                                                           <  1:377579/141‑1 (MQ=255)
ataAGATTCATATTTATCGCGATTATTACCATATAACGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCCTCTAAAACATCACTTGTTGTTGCAATAAAATCAGCTTc                                                                                                                                           <  1:572274/141‑1 (MQ=255)
ataAGATTCATATGTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTc                                                                                                                                           <  1:434215/141‑1 (MQ=255)
ataACATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGCATACATAGTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTc                                                                                                                                           <  1:735694/141‑1 (MQ=255)
 taAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCa                                                                                                                                          <  2:1034759/141‑1 (MQ=255)
 taAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGATTCa                                                                                                                                          >  2:798469/1‑141 (MQ=255)
        cATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATg                                                                                                                                   >  1:280114/1‑141 (MQ=255)
            tttATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTaa                                                                                                                               <  1:786832/141‑1 (MQ=255)
             ttATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTCCTCTTGCAATAAAATCAAGTTAAAAACATGGTAAc                                                                                                                              >  2:975677/1‑141 (MQ=255)
              tATCGCGATTATTACCATATAAAGCTTCGATATACCTAATAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACAGATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACa                                                                                                                             <  1:16241/141‑1 (MQ=255)
                 cgcgATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCCATAAAATCAACTTCAAAATATGGTAACAATc                                                                                                                          >  2:218720/1‑141 (MQ=255)
                   cgATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCACTAAAATCAGCTTCAAACTATGGTAACAATCCt                                                                                                                        >  1:1067887/1‑141 (MQ=255)
                     attattACCACATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTaa                                                                                                                      <  1:13690/141‑1 (MQ=255)
                        attaCCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAAtt                                                                                                                   >  2:880348/1‑141 (MQ=255)
                        attaCCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTACTAATGGTCGTGCTTGCGGATACATATTCTCTTCTTCTAAAACATCACTTGCTGTTGCAATAAAACCAGCTTCAAAATATAGTACCAATCCTAACtt                                                                                                                   >  1:104237/1‑141 (MQ=255)
                         ttaCCATATAAAGCTGCGATATAACTAAAAGGCTTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAAttt                                                                                                                  >  2:454809/1‑141 (MQ=255)
                           aCCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTACTAGTGGATAAATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCCGCTTCAAAATATGATAAAAAACCTAAAtttta                                                                                                                >  2:164581/1‑140 (MQ=255)
                           aCCATATAAAGCGGCGAAATAACTAAATGAATTCGGCTCTCCAGATGGTCGTGCTTGCGGATACATATTCTACGACTCTAAAGCATCACTTGCTGTTGCGATAAAATCAGCTTCAAAATATGGTAACAAACCTAAGTTTTc                                                                                                                >  2:180868/1‑141 (MQ=255)
                                     gctcggATCTAGCTAACAGTACTCTGCTTCCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAACTTCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGcaca                                                                                                      <  2:275540/136‑1 (MQ=255)
                                     gCTGCGATATAACTAAAAGGATTCGGCTTTCCTACTGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGcaca                                                                                                      >  1:700149/1‑141 (MQ=255)
                                     gCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGcaca                                                                                                      >  2:810662/1‑141 (MQ=255)
                                     gCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGcaca                                                                                                      >  1:156894/1‑141 (MQ=255)
                                      cTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCacaa                                                                                                     <  1:911785/141‑1 (MQ=255)
                                       tGCTATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCacaac                                                                                                    <  2:29235/141‑1 (MQ=255)
                                           atatAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGtc                                                                                                >  2:215132/1‑141 (MQ=255)
                                           atatAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGtc                                                                                                >  1:88367/1‑141 (MQ=255)
                                           atatAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGtc                                                                                                >  2:807920/1‑141 (MQ=255)
                                           atatAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGtc                                                                                                >  1:105464/1‑141 (MQ=255)
                                               aaCTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCATTAACAGCAGGGCCAATAGATTGTAACAATTCTAAATTTTCAAATGGCACAACACGCTCAg                                                                                            >  2:1062072/1‑141 (MQ=255)
                                               aaCTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAACATACGCTTCAAAATAAGGTCCACATCCAAAATTTTCAACTggaccagcatccccag                                                                                            >  2:213311/1‑127 (MQ=255)
                                               aaCTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTATAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTCACAATCCTAACTTTTCAAATGGCACAACAGTCTCAg                                                                                            >  2:323013/1‑141 (MQ=255)
                                                aCTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCGCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAga                                                                                           >  1:767133/1‑140 (MQ=255)
                                                 cTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGta                                                                                          <  1:22975/141‑1 (MQ=255)
                                                        gATTCTGCTTTCACAATGGTCGTGCTTGCGGATGCATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGgac                                                                                   <  2:619597/141‑1 (MQ=255)
                                                         aTTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTGTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATAGGGTCACAATCCTACGTTTTCCAATGGAGCAACAGTCCCAGTATAAGgaag                                                                                  >  1:17564/1‑139 (MQ=255)
                                                         aTTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGgacg                                                                                  >  1:390079/1‑141 (MQ=255)
                                                           tCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTCCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGgacgac                                                                                >  2:85724/1‑141 (MQ=255)
                                                           tCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTCCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGgacgac                                                                                >  2:686551/1‑141 (MQ=255)
                                                             ggCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCt                                                                              >  2:598797/1‑141 (MQ=255)
                                                             ggCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCt                                                                              >  2:734188/1‑141 (MQ=255)
                                                               cTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCACCTTCAAAATATGGTAACAATCCTAAATTTTCCAATGGCACAACCGTCTCAGTATAACGACGACCtct                                                                            >  2:940195/1‑139 (MQ=255)
                                                                 ttCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAGCAGTCTCAGTATAAGGACGACCTGTTg                                                                          >  1:909919/1‑141 (MQ=255)
                                                                 ttCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTg                                                                          >  2:505581/1‑141 (MQ=255)
                                                                 ttCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTg                                                                          >  2:28348/1‑141 (MQ=255)
                                                                 ttCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAAAGGCACAACGGTCTCAGTATAAGGACGACCTGTTg                                                                          >  1:910026/1‑141 (MQ=255)
                                                                  tCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGc                                                                         >  1:664578/1‑141 (MQ=255)
                                                                  tCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGc                                                                         >  2:770109/1‑141 (MQ=255)
                                                                  tCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTGCAAAATATGGTAACATTCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGc                                                                         >  1:478442/1‑141 (MQ=255)
                                                                  tCCTAATGGTCGTGCTTGCAGATACATATTCTCTGCTCCGAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATCTGGTAACAATCCTAAATTTTCAAATGGCACCACAGTCTCAGTATAAGGACGACCTGTTGc                                                                         >  1:587499/1‑141 (MQ=255)
                                                                      aaTGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGAGGACCTGTCGCAATa                                                                     >  1:405976/1‑141 (MQ=255)
                                                                      aaTGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATa                                                                     >  1:126440/1‑141 (MQ=255)
                                                                        tGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATAcc                                                                   >  2:1103651/1‑141 (MQ=255)
                                                                            cGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAAt                                                               <  2:891747/141‑1 (MQ=255)
                                                                            cGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAAt                                                               <  2:906742/141‑1 (MQ=255)
                                                                             gtgcgtgcGGATACGTAGTCTTCGCGTCCAAGACATCACTGGCTGTTGCAATAAAAGCAGCGTCAAAATATGGTAAGAATCCGAAATTTTCAAATGGGACAACAGTCGCAGTATAAGGACGACCTGTTGCAATACCTAAtt                                                              <  1:496069/141‑1 (MQ=255)
                                                                               gCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTca                                                            >  1:67447/1‑140 (MQ=255)
                                                                                cTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGa                                                           >  1:793373/1‑141 (MQ=255)
                                                                                cTTGCGGATACATATTCTCTGCCTCTCAAACATCTCTTGCTGTCGCAATAAAATCAGCCTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGa                                                           <  1:730888/141‑1 (MQ=255)
                                                                                 ttGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGaa                                                          <  1:1103651/141‑1 (MQ=255)
                                                                                   gCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAAc                                                        >  1:1045132/1‑141 (MQ=255)
                                                                                   gCGGATACATATTCTCTGCGTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAAc                                                        >  1:587052/1‑141 (MQ=255)
                                                                                    cGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTGGAAAcc                                                       >  2:984232/1‑141 (MQ=255)
                                                                                    cGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCCCAGTTTCAGGACGCCCTGCTGCAATACCTGATTCGACAcc                                                       >  2:270959/1‑141 (MQ=255)
                                                                                    cGGATACATATTCCCTGCTTCTAACACATCACTCGCCGTTGCAATAAAACCCGCCTCAAAATATGGTAACAATCCCAAATTTTCAAATGGCACAACAGTCTCCGTATAAGGACGACCTGTTGCAATACCTAATTCGAAAcc                                                       <  2:172432/141‑1 (MQ=255)
                                                                                      gATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCACATAGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCCAAActaa                                                     >  2:155034/1‑138 (MQ=255)
                                                                                       aTACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGc                                                    >  1:905866/1‑141 (MQ=255)
                                                                                        tACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGTACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCa                                                   <  1:714974/141‑1 (MQ=255)
                                                                                        tACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCa                                                   <  2:622883/141‑1 (MQ=255)
                                                                                          cATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTACATTTTCAAATGGCACAACAATCTCAGTATAAGGACGACCTGTTGCAATAGCGACTTCGAAACAAGCAcc                                                 >  2:292327/1‑141 (MQ=255)
                                                                                           atatTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAATCCCAGTATAAGGACGACGTGTTGCGATACCTAGTTCTAAACCAGCAACt                                                >  1:1058633/1‑141 (MQ=255)
                                                                                            tatTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCtt                                               >  1:764743/1‑141 (MQ=255)
                                                                                                  ctGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGTCCTGCTGCAATACCTAATTCGAAACCAGCACCTTTTAAAt                                         <  1:429155/141‑1 (MQ=255)
                                                                                                          aaaaCATCACTTCCTGTTGCAGTAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAATCAGCACCTTTTAAATCATTCAGa                                 >  1:571572/1‑141 (MQ=255)
                                                                                                           aaaCATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGaa                                >  2:7586/1‑141 (MQ=255)
                                                                                                            agcATCGCTTGCTGTTGCAATAAAATCAGCGTCAAAATATGGTAGCAATCGCAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGGTGCAATACCTATTTCTAAACCAGCACCTTTTAAATCATTCAGAAg                               <  2:755378/139‑1 (MQ=255)
                                                                                                            aacagaacgtGCTGATGCAATAAAATCAGCTTCAAAAGATGGTAACAATCCTAAATCTTCAAATGGCACAACAGTCTCGGTATAAGGACGACATGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAg                               <  1:213463/132‑1 (MQ=255)
                                                                                                            aaCATCACTTGCTGTTGCAATAAAACCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCCCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAg                               <  1:564213/141‑1 (MQ=255)
                                                                                                               aTCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTAc                            >  1:13016/1‑141 (MQ=255)
                                                                                                                tCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTAcc                           >  2:82566/1‑141 (MQ=255)
                                                                                                                tCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTAcc                           >  1:7250/1‑141 (MQ=255)
                                                                                                                 cACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCATCACCTTTTAAATCATTCAGAAGTACCt                          <  2:156894/141‑1 (MQ=255)
                                                                                                                 cACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCt                          <  2:700149/141‑1 (MQ=255)
                                                                                                                  aCTTGCTGTTGCAATAAAATCAGCTCCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCtt                         <  1:686551/141‑1 (MQ=255)
                                                                                                                  aCTTGCTGTTGCAATAAAATCAGCTCCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCtt                         <  1:85724/141‑1 (MQ=255)
                                                                                                                      gCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACt                     >  2:652326/1‑141 (MQ=255)
                                                                                                                       cTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACtt                    >  2:734887/1‑141 (MQ=255)
                                                                                                                        tGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAATACCTTAACTTc                   >  2:650047/1‑141 (MQ=255)
                                                                                                                           tGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTACCTTCATc                >  1:247788/1‑141 (MQ=255)
                                                                                                                            gCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCt               >  1:683215/1‑141 (MQ=255)
                                                                                                                                tAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTg           >  2:154031/1‑141 (MQ=255)
                                                                                                                                 aaaaTCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTgg          >  2:343029/1‑141 (MQ=255)
                                                                                                                                   aaTCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGAACGACAGTCTCAGTATAAGGACGACCTGTTGCAATGCCTAATTCGAAACGAGCACCTTTGAAATAATTCAGAAGTATCTTAACttattcgaatggta        >  2:808079/1‑129 (MQ=255)
                                                                                                                                    aTCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTGGtct       <  2:194919/141‑1 (MQ=255)
                                                                                                                                        gCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATCAGGACGACCGGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTGGTCTCaaa   >  1:307069/1‑141 (MQ=255)
                                                                                                                                        gCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCCTCTACTGTTCTCaaa   >  2:853793/1‑141 (MQ=255)
                                                                                                                                        gCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTGGTCTCaaa   >  1:1076018/1‑141 (MQ=255)
                                                                                                                                        gCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTGGTCTCaaa   >  1:442977/1‑141 (MQ=255)
                                                                                                                                         cTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTGGTCTCaaaa  <  2:390079/141‑1 (MQ=255)
                                                                                                                                         cTTAAAAATATGGTAACAATCCTAACTTTTCAAATGGCACAACAGTGTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTGGTCTCaaaa  <  2:1058633/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGATTCGGCTTTCCTAATGGTCGTGCTTGCGGATACATATTCTCTGCTTCTAAAACATCACTTGCTGTTGCAATAAAATCAGCTTCAAAATATGGTAACAATCCTAAATTTTCAAATGGCACAACAGTCTCAGTATAAGGACGACCTGTTGCAATACCTAATTCGAAACCAGCACCTTTTAAATCATTCAGAAGTACCTTAACTTCATCTACTGGTCTCAAAA  >  CP000730/1837282‑1837559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: