Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,091,501 G→T 4.8% Q34K (CAA→AAA)  USA300HOU_1954 ← hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,091,5010GT 4.8% 644.6 / 5.3 214Q34K (CAA→AAA) USA300HOU_1954hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base G (93/105);  new base T (11/2);  total (106/108)
Fisher's exact test for biased strand distribution p-value = 9.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

CTAAACCAAGCACCCACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTA  >  CP000730/2091363‑2091641
                                                                                                                                           |                                                                                                                                            
cTAAACCAAGCACCCACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGAc                                                                                                                                            <  1:1022310/141‑1 (MQ=255)
cTAAACCAAGCACCCACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGAc                                                                                                                                            >  1:370447/1‑141 (MQ=255)
   aaCCAAGCACCCACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGTTCTTGGGTTTTTTGACCta                                                                                                                                         >  1:927792/1‑141 (MQ=255)
   aaCCAAGCACCCACATTTTCATATCACGTATGTTCTTATCATTTTCCTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCta                                                                                                                                         >  1:1054190/1‑141 (MQ=255)
     ccAAGCCCCCACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTACTTTAATGTTAACTTGCTCTTGGGTTTTTTGACCtaat                                                                                                                                       <  1:592111/141‑1 (MQ=255)
      cAATCACCCACATTTTCCTATCGCTGATTTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATATCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCtaatt                                                                                                                                      <  2:1055514/141‑1 (MQ=255)
            cccACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAGGTTGGTGTTGGGTTTTTTGACCTaatttacttt                                                                                                                                >  1:201990/1‑141 (MQ=255)
            cccACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTGTTTTAGTTactttaattt                                                                                                                                >  2:839462/1‑141 (MQ=255)
            cccACATTTTCATACCACGTATGTTCTTATCATTTTCTTTCTTAATCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTaatttaattt                                                                                                                                >  1:603544/1‑141 (MQ=255)
               acaTTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCAt                                                                                                                             >  1:197797/1‑141 (MQ=255)
                  ttttCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGa                                                                                                                          >  1:111198/1‑141 (MQ=255)
                      cATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTAAATAGCATCTAAGGTTTTATCTAATTTATTGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTAAgtgt                                                                                                                      >  2:18899/1‑141 (MQ=255)
                       atatCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTTTTTTTTTTCTAAGGTTTTATCTAATTTCATGTTAACTTGCTCTTTGGTTTTTTTACCTAATTTATTTTCTTTGAttgta                                                                                                                     >  2:630378/1‑140 (MQ=255)
                          tCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTg                                                                                                                  <  2:622844/141‑1 (MQ=255)
                             cGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGc                                                                                                               <  1:1069627/141‑1 (MQ=255)
                               tATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCAt                                                                                                             <  2:1022638/141‑1 (MQ=255)
                                  gTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTTCCCTAATTTAATTTCATTGAGTGTGCTGAGCATTGt                                                                                                          >  1:509441/1‑141 (MQ=255)
                                   ttCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGGGTGTGCTGAGCATTGtt                                                                                                         >  1:8689/1‑141 (MQ=255)
                                      ttATCATGTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTTCTGAGCATTGTTTTa                                                                                                      <  1:819037/141‑1 (MQ=255)
                                           aTTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTTAATAACATCTAAGGTTTTATCTCATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCAtt                                                                                                 >  2:180776/1‑141 (MQ=255)
                                            ttttCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATtc                                                                                                >  1:608749/1‑141 (MQ=255)
                                               tctttcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATGTCATTGAGAGACCTGAGCATTGTTTTATCATTctct                                                                                             >  2:597011/1‑141 (MQ=255)
                                                ctttcttATTCTTTTCATCTATTTCTGTTTCTTTTTGAATAGCATCTATGGTTTTATCTAATTTAATGTTTACTTGCTCTTGGGTTTTTTAACCTAATTTAATTTCTTTGAGTGTGCTGAGCATTGTTTTATTATTCtctt                                                                                            >  2:662235/1‑141 (MQ=255)
                                                  ttcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCtcttct                                                                                          >  1:309479/1‑141 (MQ=255)
                                                  ttcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCtcttct                                                                                          >  1:416369/1‑141 (MQ=255)
                                                  ttcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATATAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATCTAATTTCATCGAGTGTTCTGAGCATTGTTTTATCATTCtcttct                                                                                          >  2:1044811/1‑141 (MQ=255)
                                                   tcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTa                                                                                         <  2:730602/141‑1 (MQ=255)
                                                   tcttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGATTTAGCGTGCTCTTCTa                                                                                         <  2:788974/141‑1 (MQ=255)
                                                    cttATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCtcttctca                                                                                        >  2:821149/1‑139 (MQ=255)
                                                     ttATTCTTTTCATCTATTTTGCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTGATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAAc                                                                                       >  1:717530/1‑141 (MQ=255)
                                                         tCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCtgc                                                                                   <  2:956722/141‑3 (MQ=255)
                                                          cTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTCATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTTATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTTTCTTCTAACCtttt                                                                                  >  1:155284/1‑139 (MQ=255)
                                                          cTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGTCCTAATTTGATTTCATTGAGTGTGCTGAGCATTGTGTTATCATTCTCTTCTAACCTTCt                                                                                  >  2:722955/1‑141 (MQ=255)
                                                          cTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCt                                                                                  >  1:1086081/1‑141 (MQ=255)
                                                           ttttCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTg                                                                                 <  2:111198/141‑1 (MQ=255)
                                                             ttCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTTTTTTTTTTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTTACCTTCTGAt                                                                               >  2:1039759/1‑141 (MQ=255)
                                                             ttCATCTATTTCTCATTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTAACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGAt                                                                               >  2:755490/1‑141 (MQ=255)
                                                                aTCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATgcg                                                                            >  2:81925/1‑141 (MQ=255)
                                                                   tATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTACGTGTGCTGAGCATTGTTTTATCATCCTCTTATAACCTTCTGATGCGCCa                                                                         <  2:812679/141‑1 (MQ=255)
                                                                     tttCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTACTTTGATTTCATTGCGTGTGCTGAGCATTGTTTTATCCTTCTGCTCTAACCTTCTGCTgcgcattt                                                                       >  2:273198/1‑137 (MQ=255)
                                                                      ttCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTc                                                                      <  2:526654/141‑1 (MQ=255)
                                                                         tctttcttTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATg                                                                   <  2:197797/141‑1 (MQ=255)
                                                                            ttctttTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTc                                                                <  2:766511/141‑1 (MQ=255)
                                                                                  tttttttttttttAAGGCTTAATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTACTTTAATTTCTTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCTTGTTCGTGttt                                                          >  2:184127/13‑141 (MQ=255)
                                                                                  ttGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTtttt                                                          >  2:168593/1‑141 (MQ=255)
                                                                                     aaTAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTTCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGtttttt                                                       <  1:465648/141‑1 (MQ=255)
                                                                                      agaGCATCTAAGGTTTTATCTAATTTAATGTTTACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTg                                                      <  2:425871/139‑1 (MQ=255)
                                                                                      aTAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTg                                                      <  2:570400/141‑1 (MQ=255)
                                                                                       tAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGa                                                     <  1:550337/141‑1 (MQ=255)
                                                                                        aGCATCTAAGGTTTTATCTAATTTAGTGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGaa                                                    >  2:1019543/1‑141 (MQ=255)
                                                                                        aGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTTTTCTATTTTAATTTCATAGACTGTGCTGAGCATTGTTTTATCATTCTCGTCTAACCATCTGATGCGCCCTTCATGCTCGTGTGTTTTgca                                                    >  2:28694/1‑139 (MQ=255)
                                                                                        aGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGaa                                                    >  1:398154/1‑141 (MQ=255)
                                                                                         gCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAac                                                   >  2:332886/1‑141 (MQ=255)
                                                                                                aGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCa                                            >  1:97712/1‑141 (MQ=255)
                                                                                                 ggTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCaa                                           >  2:935215/1‑141 (MQ=255)
                                                                                                 ggTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGAGTGCTGAGAATTTTTTTATCATTCTCTTCTCGACTTCTGTTTCGCACTGCCCGTCCGGGTCTTTTGACACACCCCaa                                           >  2:903187/1‑141 (MQ=255)
                                                                                                  gTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAAACTTTTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAAt                                          >  1:852191/1‑141 (MQ=255)
                                                                                                     ttATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCa                                       >  1:448825/1‑141 (MQ=255)
                                                                                                      tATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAg                                      <  2:317641/141‑1 (MQ=255)
                                                                                                      tATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAg                                      <  2:513936/141‑1 (MQ=255)
                                                                                                      tATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAg                                      <  1:960916/141‑1 (MQ=255)
                                                                                                       ttCTAATTTAATGTTATCTTGCTCTTGGGTTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAg                                      <  2:481975/140‑1 (MQ=255)
                                                                                                          tAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTaca                                  >  1:882094/1‑141 (MQ=255)
                                                                                                           aaTTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTacac                                 >  1:726350/1‑141 (MQ=255)
                                                                                                              ttAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCg                              <  2:536323/141‑1 (MQ=255)
                                                                                                               tAATGTTAACTTGTTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCCTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCGATTCAGTACACCCGc                             <  1:824771/141‑1 (MQ=255)
                                                                                                               tAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGc                             <  1:740388/141‑1 (MQ=255)
                                                                                                                aaTGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCt                            <  2:875869/141‑1 (MQ=255)
                                                                                                                 aTGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCGAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCtt                           >  1:745416/1‑141 (MQ=255)
                                                                                                                 aTGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGCGTGCAGAGCATTGATATATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGCACCACCCCAATCCACTACACCCGCtc                           >  2:699590/1‑140 (MQ=255)
                                                                                                                 aTGTAAACTTGCACTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTTAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCCCTCCCATTCAGTACACCCGCta                           >  2:673199/1‑140 (MQ=255)
                                                                                                                  tGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCttt                          <  2:752745/141‑1 (MQ=255)
                                                                                                                   gTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTc                         >  1:1026020/1‑141 (MQ=255)
                                                                                                                     tAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTa                       <  2:642646/141‑1 (MQ=255)
                                                                                                                     tAACTTGCTCTTGGGTTTTTTCACCTAATTTAATTTCATTCAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCTTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTa                       <  2:239450/141‑1 (MQ=255)
                                                                                                                      aaCTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAGCCACCCCAATTCAGTACACCCGCTTTCTaa                      >  1:357091/1‑141 (MQ=255)
                                                                                                                       aCTTGCTCTTTGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTTCACCCGCTTTCTcaa                     <  2:927792/141‑1 (MQ=255)
                                                                                                                         ttGCTCTTGGGTTTTTTGACCTAGTTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTATCTTCTAACCTTCTGATGCGCCATTAATGTTCGTGTTTTTTGAAGTACAGCAACTCAGTCCTGCCGCTTTCTacata                   >  1:418442/1‑137 (MQ=255)
                                                                                                                         ttGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAg                   <  2:653314/141‑1 (MQ=255)
                                                                                                                         ttGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAg                   <  1:1047138/141‑1 (MQ=255)
                                                                                                                         ttGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAg                   <  1:879736/141‑1 (MQ=255)
                                                                                                                          tGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCTATTCAGTACACCCGCTTTCTAAAAGa                  >  2:567734/1‑141 (MQ=255)
                                                                                                                            ctctTGGGTTTTTTTTTTTTTTTTTATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGTTGCGCCATTCATGTTCGTGTTTTTTGTACCGCCCCACTTCTGTTTACCCGCTTTCTaaatgctt                >  2:1046363/1‑136 (MQ=255)
                                                                                                                            ctctTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAATCACCCCACTTCAGTGCACCCGCTTTCTAAAAGAAt                >  2:381366/1‑141 (MQ=255)
                                                                                                                            caattGGGTTGTGTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGAAGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTATAAAAGaaa                >  2:402858/4‑140 (MQ=255)
                                                                                                                             tctTTGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATa               <  1:947456/141‑1 (MQ=255)
                                                                                                                             tcgtGGGGTTTTTGACCTAATTTAATTTCATTGCGTCTGCTGACTATTGTTTTATCATTCTCTTCTAACCTTCTCATGCGCCATCTATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACCCCCGCGTTCTACACGAATa               <  2:499742/138‑1 (MQ=255)
                                                                                                                              ctTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCCTTAATGATCATGTTGGTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATaa              <  2:1063978/141‑1 (MQ=255)
                                                                                                                              ctTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGTATaa              <  1:755490/141‑1 (MQ=255)
                                                                                                                               ttGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCCTTCGCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTGAAAGAATaaa             <  1:802993/141‑1 (MQ=255)
                                                                                                                               ttGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATaaa             >  1:1006885/1‑141 (MQ=255)
                                                                                                                                  ggTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGAt          >  1:894746/1‑141 (MQ=255)
                                                                                                                                   gTTTTTGGACCTAATGTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCGAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGAtt         <  1:24556/141‑1 (MQ=255)
                                                                                                                                     ttttttGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCACTCCACCCGCTTTCTAAAAGACTAAAGATTa        >  1:978754/1‑141 (MQ=255)
                                                                                                                                        tttGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCCCCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATgg     >  1:938309/1‑140 (MQ=255)
                                                                                                                                        tttGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGCATCAAGATTATGa     >  1:742269/1‑141 (MQ=255)
                                                                                                                                         ttGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACTAACTCTTTCTAAAAGAATAAAGATTATGAg    <  1:812955/141‑1 (MQ=255)
                                                                                                                                         ttGACCTAATTTAATTTCATTGAGGGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTCCACCCGCTTTCTAAAAGAATAAAGATTATGat    >  1:147160/1‑140 (MQ=255)
                                                                                                                                           gACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTa  >  1:351980/1‑141 (MQ=255)
                                                                                                                                           gACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTa  <  1:655993/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CTAAACCAAGCACCCACATTTTCATATCACGTATGTTCTTATCATTTTCTTTCTTATTCTTTTCATCTATTTCTCTTTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACACCCGCTTTCTAAAAGAATAAAGATTATGAGTA  >  CP000730/2091363‑2091641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: