Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,814 Δ1 bp 32.7% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8140A.32.7% 333.0 / 200.1 156intergenic (+45/+63)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (56/48);  new base . (20/31);  total (76/80)
Fisher's exact test for biased strand distribution p-value = 9.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 161 total aligned reads displayed.

ATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATA  >  CP000730/35675‑35954
                                                                                                                                            |                                                                                                                                            
atGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAg                                                                                                                                             <  2:469190/141‑1 (MQ=255)
   cAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAg                                                                                                                                          >  1:182745/1‑141 (MQ=255)
       ttattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTc                                                                                                                                      <  1:105874/141‑1 (MQ=255)
        tattCTTAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCt                                                                                                                                     <  2:307282/141‑1 (MQ=255)
        tattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCt                                                                                                                                     <  1:94707/141‑1 (MQ=255)
                aTTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGAGTACTTGAACAAGAGCTAAAATAAAATTTTGATCTAATAAAAATAGAAGGTTCTGTTGCaaa                                                                                                                             <  2:342940/141‑1 (MQ=255)
                 tttAAAGATGTAGATTATCTCATTGGCAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACCAGCCCTCAACTTAACTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAg                                                                                                                            <  2:861079/141‑1 (MQ=255)
                  ttAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATCAAAATAGAAGGTTCTGTTGCAAAGt                                                                                                                           <  2:456273/141‑1 (MQ=255)
                    aaaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAGGTaa                                                                                                                         >  2:59788/1‑141 (MQ=255)
                      aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                       >  1:106535/1‑141 (MQ=255)
                      aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                       >  1:320964/1‑141 (MQ=255)
                      aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                       >  2:274314/1‑141 (MQ=255)
                      aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTAGTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATATAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                       >  1:533254/1‑141 (MQ=255)
                        aTGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAACTTGTGATCTAATAAAAATAAAAGGTACTGTTGCAAAGTaaaaaa                                                                                                                     >  2:498331/1‑141 (MQ=255)
                                     cATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCa                                                                                                        >  1:437310/1‑141 (MQ=255)
                                     cATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCa                                                                                                        >  2:220618/1‑141 (MQ=255)
                                     cATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCAACAAATGATTAGTTGAACAAGAGATCAAAACGCTCATGCAACATAGAAAAATAGAATGTACTGTTGCAAAGTAAAAAAATATATCTAACCa                                                                                                        >  2:198453/1‑141 (MQ=255)
                                           cAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAtt                                                                                                  <  1:309789/141‑1 (MQ=255)
                                              aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                               >  2:65946/1‑141 (MQ=255)
                                                   ttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTGACCACTAATTTATCATGt                                                                                          >  1:706363/1‑141 (MQ=255)
                                                   ttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGt                                                                                          >  1:797042/1‑141 (MQ=255)
                                                    tAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAACAATATATAGCTAACCACTAATTTATCATGTc                                                                                         <  2:909844/141‑1 (MQ=255)
                                                      aTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAg                                                                                       >  1:862593/1‑141 (MQ=255)
                                                         tAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGGTTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                    <  2:617546/141‑1 (MQ=255)
                                                         tAGAAGCTATTCAGTATAAAGTTTTTGGAGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                    <  2:14991/141‑1 (MQ=255)
                                                            aaGCTATTTGTTATAAAGTTTTGGGTGAAAAATTATTACTTGAACAAGAGCTAAAATAAAATTGTTATCTAATAAAAATAGAAGGTGCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                 <  1:192798/141‑1 (MQ=25)
                                                                 aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                            >  2:490116/1‑141 (MQ=255)
                                                                 aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                            >  1:54521/1‑141 (MQ=255)
                                                                 aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                            >  2:341992/1‑141 (MQ=255)
                                                                 aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCACGTCAGTGTTCGCTaaa                                                                            >  2:87173/1‑141 (MQ=255)
                                                                  ttCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTTATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTGAAc                                                                           >  2:631557/1‑141 (MQ=255)
                                                                   tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                          >  1:251862/1‑141 (MQ=255)
                                                                       tataAAGTTTTTGGCGAAAAATGATTACCTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTCCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                      <  1:654757/141‑1 (MQ=255)
                                                                        ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTa                                                                     >  1:910446/1‑141 (MQ=255)
                                                                        ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTa                                                                     >  1:757946/1‑141 (MQ=255)
                                                                              tttttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTGAGAAAAAATCGCTAACCGCTAATGTATCATGTCCGTGGTAGCGTAACTTGCTGGCatga                                                               >  1:806520/1‑141 (MQ=255)
                                                                                tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                             >  2:778364/1‑141 (MQ=255)
                                                                                gttGGCGAACCATGACTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                             >  2:914591/2‑141 (MQ=255)
                                                                                 ttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGc                                                            >  2:218511/1‑141 (MQ=255)
                                                                                   ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                          >  1:588594/1‑141 (MQ=255)
                                                                                     cGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGATCTGTTGCAAAGTGAAAAAATATAATACTTAAACAATGATTCATGTCAGTGTTGGTTTAACTTGCTAGCATGATGCTAAt                                                        >  1:680910/1‑141 (MQ=25)
                                                                                       aaaaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTATGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAAttt                                                      >  1:806204/1‑141 (MQ=255)
                                                                                          aaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                   >  1:157799/1‑141 (MQ=255)
                                                                                           aTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                  <  1:356985/141‑1 (MQ=255)
                                                                                             gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                >  1:69647/1‑141 (MQ=255)
                                                                                             gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                >  2:893729/1‑141 (MQ=255)
                                                                                                tACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATg                                             >  2:159782/1‑141 (MQ=255)
                                                                                                     gAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                        <  1:127694/141‑1 (MQ=255)
                                                                                                        cAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAt                                     <  1:878523/141‑1 (MQ=255)
                                                                                                        cAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAt                                     <  1:321502/141‑1 (MQ=255)
                                                                                                          agagGTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                   <  2:890240/141‑1 (MQ=255)
                                                                                                          agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                   >  1:179737/1‑141 (MQ=255)
                                                                                                          aaagataaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                   <  2:540250/136‑1 (MQ=255)
                                                                                                            agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                 >  1:457827/1‑141 (MQ=255)
                                                                                                            agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTACTGTTGCAAAGTAAAAAAATATGGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGCTGCTAATTTCGTGCAATGGAGAAAACCCga                                 >  2:777517/1‑140 (MQ=18)
                                                                                                              cTAAAATAAAATTGTGATCTAATAAAAATACAAGGCTCTGTTGAAAAGTAAAAAAATATAGCTAACCACTAATTTATCACGTCAGTGTAAGCTTCACATACTAGCATGATGCTAGTTTCGTGGCATATCGACAAtacttaa                               >  2:261647/1‑135 (MQ=17)
                                                                                                                    acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                        >  1:814929/15‑141 (MQ=11)
                                                                                                                     caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                       <  1:279027/128‑1 (MQ=11)
                                                                                                                     caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                       <  1:875796/128‑1 (MQ=11)
                                                                                                                     caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                       >  2:351325/14‑141 (MQ=11)
                                                                                                                      aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAACTATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                      <  2:839020/129‑1 (MQ=11)
                                                                                                                       aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAACTGAagag                     >  1:241131/12‑141 (MQ=11)
                                                                                                                        aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCACGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                    >  2:829369/11‑141 (MQ=11)
                                                                                                                         agattattttaataaAAATGATGGTTCTGGTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGGTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  2:113970/132‑1 (MQ=11)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  1:724737/132‑1 (MQ=11)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  1:529405/132‑1 (MQ=11)
                                                                                                                         agagtattttaaaaaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACAAATCTACCATGTCAGTGTCCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  1:362145/132‑1 (MQ=11)
                                                                                                                          tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  1:852574/141‑1 (MQ=255)
                                                                                                                          tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAGTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTTGATCGGGAGAGAc                   <  2:437310/141‑1 (MQ=14)
                                                                                                                          gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTATTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAGTCCGTAGATCTGGAGAGAcc                  <  1:594572/133‑1 (MQ=11)
                                                                                                                          gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  >  1:881506/9‑141 (MQ=11)
                                                                                                                          gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGAcc                  >  2:742836/9‑141 (MQ=11)
                                                                                                                          aagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTGATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  <  2:644793/133‑1 (MQ=11)
                                                                                                                           ttgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  <  1:506877/140‑1 (MQ=255)
                                                                                                                           gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  <  1:136210/141‑1 (MQ=255)
                                                                                                                           gtgAGCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  <  1:193755/141‑1 (MQ=255)
                                                                                                                             tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc               >  2:671629/6‑141 (MQ=11)
                                                                                                                             gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTg                <  1:505047/141‑1 (MQ=255)
                                                                                                                              attttaataaAAGTGATGGTTCTGTTGCACAGTAAAAAATATCGCTAATAACTCATATATGATGTCAGTGTTAGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAAATCTGAAGAGACCAGCg              >  2:55785/5‑141 (MQ=11)
                                                                                                                              attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTTATTTCGTGGCATGGCGAAAATCCGTAGATCTTAAGAGACCTGCg              >  1:617291/5‑141 (MQ=11)
                                                                                                                              attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg              <  2:926098/137‑1 (MQ=11)
                                                                                                                              attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg              <  1:402251/137‑1 (MQ=11)
                                                                                                                              attttaataaAAATGATGGTTCGGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTGTAACTTGCTAGCGTGATGCTAAGTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg              <  2:48475/137‑1 (MQ=11)
                                                                                                                               ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAAGTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg             >  2:428652/4‑141 (MQ=11)
                                                                                                                               ttttaataaAAATGATGGTTCTGTGGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg             >  2:712141/4‑141 (MQ=11)
                                                                                                                               tttcaataaAAATGATGATTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTACTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg             <  2:632873/137‑1 (MQ=11)
                                                                                                                               ctCTAATAGAAATAAAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc               <  2:605654/140‑1 (MQ=255)
                                                                                                                                tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt            <  1:789501/139‑1 (MQ=11)
                                                                                                                                ttgaataaAAATGAGGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATACATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTgatcg            <  2:244934/138‑5 (MQ=11)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt           <  2:751264/140‑1 (MQ=11)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt           <  1:613861/140‑1 (MQ=11)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGCAGATATGAAGAGACCTGCGGtt           >  2:45472/2‑141 (MQ=11)
                                                                                                                                  taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc          <  2:30591/141‑1 (MQ=11)
                                                                                                                                   aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGGGACCTGCGGTTCt         >  1:265723/1‑141 (MQ=11)
                                                                                                                                   aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAAGCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt         <  2:75171/141‑1 (MQ=11)
                                                                                                                                     taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt       <  2:667361/141‑1 (MQ=11)
                                                                                                                                      aaaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt        >  1:777612/1‑141 (MQ=37)
                                                                                                                                          aTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTta    <  1:886752/141‑1 (MQ=33)
                                                                                                                                            aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTTCCTGAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata  <  1:218511/141‑1 (MQ=12)
                                                                                                                                            aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata  >  1:442554/1‑141 (MQ=32)
                                                                                                                                            |                                                                                                                                            
ATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATA  >  CP000730/35675‑35954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: