Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,865:1 +C 13.0% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8651.C13.0% 830.5 / 142.3 230intergenic (‑948/‑220)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (104/96);  new base C (14/16);  total (118/112)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-02

Only 100 of 233 total aligned reads displayed.

GTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAA  >  CP000730/1929726‑1930005
                                                                                                                                              |                                                                                                                                                 
            cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTCCTTGTATATTTGCCGATGATATAATAGTAGCGTCGCCCATCTCTCCGGCGTCCATTTAGACGCAGAGAGGAGGTG‑TA‑TAG‑G‑G‑A‑GA‑TGC‑TTAtt                                                                                                                                 >  2:609540/1‑141 (MQ=255)
               tCTATCAGCTAGAATCATATAACTTCACAGAAGAAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑T‑GC‑TGC‑TTCTTTTc                                                                                                                              >  2:839342/1‑141 (MQ=255)
                  aTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTTTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGtt                                                                                                                           <  1:392400/141‑1 (MQ=255)
                   tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTc                                                                                                                          <  1:945882/141‑1 (MQ=255)
                          gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCAcatcat                                                                                                                   >  1:136038/1‑141 (MQ=255)
                                        aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGtcatca                                                                                                     >  1:500526/1‑141 (MQ=255)
                                            atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                >  1:403493/15‑141 (MQ=255)
                                             tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                               >  1:800637/14‑141 (MQ=255)
                                              cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                              >  1:809445/13‑141 (MQ=255)
                                              cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                              <  2:407656/129‑1 (MQ=255)
                                              cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                              <  1:273132/129‑1 (MQ=255)
                                               ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                             <  1:698084/130‑1 (MQ=255)
                                               ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                             <  1:532799/130‑1 (MQ=255)
                                                gaagtagatatgCTTGTATATTCACAGGTGGTACAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                            <  1:229031/131‑1 (MQ=255)
                                                gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGGCATCAGTGGCtgtg                                                                                            <  1:615758/131‑1 (MQ=255)
                                                gaagtagagtttcTTGGATATTCACAGTTGGTATAATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                            <  1:142484/129‑1 (MQ=255)
                                                  aGTGAATATGCTTGTATATTCATAGATGGTATAATAATAGTGTCGTCTATCTCTCAGGCGTCAATT‑‑GACGAAGTGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                          <  2:213878/141‑2 (MQ=255)
                                                   gtagatatgattGTATCTTCACCGATGGTATAGGAATAGAGTCGCTTATCGCTCAGGGGGCAATT‑‑GACGAAGAGAGGAGGGG‑CA‑TTA‑C‑T‑TGGC‑TAA‑TCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                         <  2:890004/131‑3 (MQ=255)
                                                   gtagatatgCTTGTATATTCACAGCTGGTAGAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑T‑TG‑CGAATCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                         <  2:591319/134‑3 (MQ=255)
                                                   gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                         <  1:583158/134‑3 (MQ=255)
                                                   gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                         >  2:69433/8‑139 (MQ=255)
                                                    tagatctgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTCCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                        <  2:23786/135‑4 (MQ=255)
                                                     agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                       >  1:745034/6‑137 (MQ=255)
                                                      gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                      >  1:128562/5‑136 (MQ=255)
                                                      aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TG‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCAGATACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                       >  1:489748/1‑141 (MQ=255)
                                                      aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                       <  2:529880/141‑1 (MQ=255)
                                                      aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                       >  2:723604/1‑141 (MQ=255)
                                                       atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                                     <  1:510148/138‑7 (MQ=255)
                                                        tgtgCTTGTATATTTGCCGATGATATAATCGTAGCGTCGACTATCAAAAGGGAGTAAGATGATGCGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                     <  2:198155/141‑1 (MQ=255)
                                                        tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAAAT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                    >  1:568540/3‑141 (MQ=255)
                                                         atgCTTGTATATTCACAGATGGTATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑G‑T‑T‑GC‑TAA‑TCATTTTGGTTCGCATCATAGAAACCACCATCAGTGGCTGTGctggtgcgt                                                                                   >  2:704367/2‑133 (MQ=255)
                                                         atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATTAGTGGCGGTGCTGTTGCGt                                                                                   >  2:623953/2‑141 (MQ=255)
                                                         atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtttcgt                                                                                   >  2:141103/2‑133 (MQ=255)
                                                         atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                   >  2:936563/2‑141 (MQ=255)
                                                         atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                   >  2:469908/2‑141 (MQ=255)
                                                         atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                   >  1:829767/2‑141 (MQ=255)
                                                           gCTTGTCTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                 <  2:801468/141‑3 (MQ=255)
                                                               gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                             <  1:658777/141‑7 (MQ=255)
                                                                tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                            <  1:439643/141‑8 (MQ=255)
                                                                tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                            <  1:758781/141‑8 (MQ=255)
                                                                tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                            <  2:436408/141‑8 (MQ=255)
                                                                 atattcaaagatggtATAATAATAGAGTCGCCTCTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                                           >  2:344770/10‑136 (MQ=255)
                                                                  tcttcacagatggtATAATAATAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                          <  1:867040/133‑1 (MQ=255)
                                                                    ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAAGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                        >  1:604855/7‑141 (MQ=255)
                                                                     tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                       >  2:193956/6‑141 (MQ=255)
                                                                     tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                       >  1:300299/6‑141 (MQ=255)
                                                                     tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                       >  2:428152/6‑141 (MQ=255)
                                                                        cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                    <  1:296647/139‑1 (MQ=255)
                                                                        cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGc                                                                    >  1:466031/3‑141 (MQ=255)
                                                                         agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                   <  2:872901/140‑1 (MQ=255)
                                                                          gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                  >  1:234033/1‑140 (MQ=255)
                                                                          gatggtATAATAACAGGGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                  <  2:730648/141‑2 (MQ=255)
                                                                          gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                                                   <  1:544157/141‑1 (MQ=255)
                                                                           atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                  <  2:69720/141‑1 (MQ=255)
                                                                            tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGTGTATTATACTTATTGGCttag                                                                >  1:750865/4‑138 (MQ=255)
                                                                            tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                <  1:106099/138‑4 (MQ=255)
                                                                            tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTCCGTTTTTTTCTTATTGGCCaa                                                                 >  1:169448/1‑141 (MQ=255)
                                                                             ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               >  1:204431/3‑141 (MQ=255)
                                                                             ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               >  1:42751/3‑141 (MQ=255)
                                                                             ggtacaataatAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTAGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               <  2:566423/136‑1 (MQ=255)
                                                                               tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTC‑CA‑TTA‑C‑TTT‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCGTATTATACTTATTGGCtgaataa                                                             >  1:632710/1‑135 (MQ=255)
                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgtaaa                                                            >  2:293752/1‑134 (MQ=255)
                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                            >  1:847028/1‑139 (MQ=255)
                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                            >  1:4748/1‑139 (MQ=255)
                                                                                 taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                           <  1:772575/141‑4 (MQ=255)
                                                                                   ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                         <  1:184848/141‑1 (MQ=255)
                                                                                   ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                         <  1:38992/141‑1 (MQ=255)
                                                                                   ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTG‑C‑T‑T‑TACTAA‑GCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGTCTTAGTAAAccg                                                         >  2:261080/1‑139 (MQ=255)
                                                                                      atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCTGTTGCTTTGCTGTTGCGTATTATATTTATTGGCTTATTAAACGCaac                                                      >  2:147231/2‑140 (MQ=255)
                                                                                        agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGTACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                    <  2:932192/141‑4 (MQ=255)
                                                                                        agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                    >  2:682593/1‑138 (MQ=255)
                                                                                         gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                    <  2:928912/141‑1 (MQ=255)
                                                                                         gagTCGCCTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                   <  1:733450/141‑5 (MQ=255)
                                                                                          agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                  >  1:704799/1‑136 (MQ=255)
                                                                                          agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCACTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                  <  1:625419/141‑6 (MQ=255)
                                                                                          agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                  <  1:687557/141‑6 (MQ=255)
                                                                                             cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                               >  1:929096/1‑133 (MQ=255)
                                                                                             cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                               >  2:272426/1‑133 (MQ=255)
                                                                                              gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                              >  2:743584/1‑132 (MQ=255)
                                                                                              gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                              <  2:134626/141‑10 (MQ=255)
                                                                                               ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                              >  2:891788/1‑141 (MQ=255)
                                                                                                cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaccaaataattaa                                            >  1:354737/1‑129 (MQ=255)
                                                                                                cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                            <  2:864551/141‑12 (MQ=255)
                                                                                                cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                            >  1:322458/1‑130 (MQ=255)
                                                                                                 tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCAA‑TAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                           >  1:526362/1‑129 (MQ=255)
                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGTTTAGTAAACGCaacaaataattgata                                          >  1:714068/1‑128 (MQ=255)
                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTGCACATCATAGCACCAGTCATGAGTGGCTGTGCTGTTGCGTATTATACTGATTGGCTTAGTAGACGCaacaaataattaaaa                                          >  1:95698/1‑128 (MQ=255)
                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattcata                                          >  2:327059/1‑128 (MQ=255)
                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GA‑TAA‑TCATTATCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                          >  2:649384/1‑128 (MQ=255)
                                                                                                   tctctcATGGGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                         <  2:365915/141‑15 (MQ=255)
                                                                                                   tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTGGACGCAATACAGAAtttgtg                                          >  2:657552/1‑136 (MQ=255)
                                                                                                         atgCGTAAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGGTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACatat                                    <  1:550462/139‑1 (MQ=255)
                                                                                                          ggCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata                                   <  2:398467/141‑1 (MQ=255)
                                                                                                           gCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTTGATGCAGTACAAAATAGGTGACATATAg                                  >  1:679796/1‑141 (MQ=255)
                                                                                                                aaTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                             <  1:101620/141‑1 (MQ=255)
                                                                                                                      gACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa                       <  1:883763/141‑1 (MQ=255)
                                                                                                                      gACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa                       <  1:781378/141‑1 (MQ=255)
                                                                                                                            gagagGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATc                 >  1:441603/1‑141 (MQ=255)
                                                                                                                                ggaggTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt             >  1:348371/1‑141 (MQ=255)
                                                                                                                                             a‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGCCATATAGCCGCACCAATAAAAATCCCCTCACCACCGCaa  >  2:514066/1‑141 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAA  >  CP000730/1929726‑1930005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑