Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,378 C→T 90.3% L58L (TTG→TTA USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3780CT90.3% 2044.9 / 195.5 1012L58L (TTG→TTAUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (53/45);  new base T (446/456);  total (507/505)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 1012 total aligned reads displayed.

CAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAC  >  CP000730/36240‑36515
                                                                                                                                          |                                                                                                                                         
 aaCCAAATATCCAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                                                                                        <  1:269629/141‑1 (MQ=255)
  aCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                       <  2:361056/141‑1 (MQ=255)
  aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                       >  2:164493/1‑141 (MQ=38)
   ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAATTTTGCTATAAAAt                                                                                                                                      >  2:73029/1‑141 (MQ=11)
   ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                      >  1:198743/1‑141 (MQ=255)
      aaTAACTAATGTATGTACCTCTGTATAAATGGCCCGCTATAACTATCTCCATTTTCCTTTTAATTTGATATACGTCACATCACTACGGCTTTTGTAATAAAATTTTTTATGCTTTTTCTTCCAAATTTAACATCAAATTgg                                                                                                                                   >  2:258124/1‑141 (MQ=2)
        tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATATAAAATTgggg                                                                                                                                 <  1:560419/141‑1 (MQ=255)
        tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATAAGACATTTGTAATAAGCTTTTTTGTGCTTTTTCTTCCTAATTTGATATAAAATTtggg                                                                                                                                 >  1:786191/1‑141 (MQ=11)
          tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                                                                               <  1:944137/141‑1 (MQ=255)
           cTAATGTTTGTCCCTTTGGATCAATGGCACGATATGAATAGCTCCATTTTCCTTTTATTTTGATGGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                              <  1:540774/141‑1 (MQ=11)
           cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                              <  1:166398/141‑1 (MQ=255)
           cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCat                                                                                                                              <  2:479050/141‑1 (MQ=255)
              atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATAtt                                                                                                                           <  2:208326/141‑1 (MQ=255)
               tgtatgtCCCTCTGTATCAATGGCACGATATTCATCGCCCTATCTGCCGTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCATATAAAATTGGGGCATATTc                                                                                                                          <  1:489102/141‑1 (MQ=11)
               tgtatgtCCCTCTGCATCAATGGCAAGAAATAAATAGCTCCATTGTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTc                                                                                                                          >  2:839373/1‑141 (MQ=255)
                gtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                         <  2:373548/141‑1 (MQ=37)
                  atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATATTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                       <  1:668002/141‑1 (MQ=11)
                   tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTTATCAATACGCCTTTTGTAATAAGATTTTTTATGCTTTTTCTTCCAAATTTGATCGAAAAGTGGTGTATATTCttca                                                                                                                      >  1:102979/1‑139 (MQ=14)
                     tcctttggCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                    <  2:650353/134‑1 (MQ=255)
                     tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCGGAAGAATTTGGGGCATATTCTTGAAc                                                                                                                    >  1:122449/1‑141 (MQ=11)
                     tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                    >  1:800359/1‑141 (MQ=21)
                        ctctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACATCTCATCCATACGCCATTTGTATTAAGCTTTTTTATGGTTTTTCTTGCAAATTTAATAAAAAATTGGGGCTTTTTTGTGAAACCa                                                                                                                 >  1:228445/1‑141 (MQ=11)
                          ctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                                                                               >  1:589307/1‑141 (MQ=21)
                          ctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTAGAACGCAAc                                                                                                               >  1:733855/1‑141 (MQ=255)
                            gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTGCTTCCAAATTTGATATAGAATTGGGGCATATTCTTGAACCCAACgg                                                                                                             >  1:144995/3‑141 (MQ=11)
                            gtaTCAATGGCACGATATAAATAGCTCCATTTCCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCATATTCTTGAACCCAACgg                                                                                                             >  1:387870/3‑141 (MQ=11)
                             taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                            >  1:896423/2‑141 (MQ=255)
                             cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCTCATAAATACGCCATTTGTAATACGCTTTTTTATGCTTTTTCTTGCAAATTTGATCTAAAATTGGGGCATATTTTTGAACCCTACGGt                                                                                                            >  1:917619/1‑141 (MQ=11)
                             cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCTTATTCTTGAACCCAACGGt                                                                                                            >  1:340485/1‑141 (MQ=255)
                              aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                                                                                           >  2:749500/1‑141 (MQ=255)
                              aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATGGGGGCATATTCTTGAACCCAACGGtc                                                                                                           <  2:586347/141‑2 (MQ=16)
                               tCAATGGCCCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACATCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                          <  2:608365/141‑1 (MQ=11)
                               tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAg                                                                                                          >  1:60742/1‑141 (MQ=255)
                                cAATGGCACGATAAAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                         <  1:58008/141‑1 (MQ=255)
                                  aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatc                                                                                                       <  1:841864/141‑3 (MQ=16)
                                     gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                    >  1:309778/1‑141 (MQ=255)
                                       aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                  <  1:831396/141‑1 (MQ=255)
                                       aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                  >  1:182004/1‑141 (MQ=255)
                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGGTTTTACTTCTAACTTTGATACAAAATTGTGTCATATTCTTGAACCCACCAGTAGACCGTTGaa                                                                                                 >  2:417290/1‑141 (MQ=14)
                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTTGACCGTTGaa                                                                                                 >  2:701511/1‑141 (MQ=21)
                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                 >  2:827536/1‑141 (MQ=255)
                                         gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                                >  2:110528/1‑141 (MQ=255)
                                         gATATAAATAGCTCCATTTTCCTTTTAGTTGGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGTAAATTTGCTATAAAATTGGGGCATATTCTTGAACCCAACGGCAGCTCGTTgtaa                                                                                                >  2:276805/1‑138 (MQ=11)
                                           tataAATAGGTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                              <  2:180998/141‑1 (MQ=255)
                                            ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                                                                             >  2:132047/1‑141 (MQ=255)
                                              aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACACAACGGTAGATCGTTGCatggtta                                                                                           >  1:210470/1‑137 (MQ=11)
                                               aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                                                                                          >  1:823499/1‑141 (MQ=21)
                                                 tAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                        <  2:793171/141‑1 (MQ=255)
                                                   gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                      <  1:780858/141‑1 (MQ=255)
                                                      ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                                                                                   <  2:875549/141‑1 (MQ=18)
                                                        agttttCTTTTATTTTGATATACGTTTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                 <  2:151994/139‑1 (MQ=11)
                                                        aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                 <  2:858799/141‑1 (MQ=255)
                                                        aTTTTCCTTTTATTTGGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                 <  2:225930/141‑1 (MQ=18)
                                                         ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                <  1:403673/141‑1 (MQ=21)
                                                         ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                <  2:465143/141‑1 (MQ=18)
                                                         ttttCCTTTTATTTTGATATACTTTTCATCAATACGCTATTTGTAATACGCTTTTTTATTCTTTTTCTTCCAAATTTGATATTAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                <  1:358754/141‑1 (MQ=11)
                                                         ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                >  2:23921/1‑141 (MQ=255)
                                                         ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                <  1:466321/141‑1 (MQ=255)
                                                         ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTCTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGCTGAATGATGAACGTTTAcacc                                                                                >  2:5326/1‑141 (MQ=11)
                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                            >  1:812188/1‑141 (MQ=40)
                                                              cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                           <  2:932229/141‑1 (MQ=255)
                                                              cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                           <  1:68107/141‑1 (MQ=255)
                                                               ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTc                                                                          <  2:779329/141‑1 (MQ=18)
                                                                  tagtttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATCCTTTTAGTCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                       <  1:413449/138‑4 (MQ=11)
                                                                   atcttGATATACGTCTCATCAATACGCCATTTGTAATAATCGTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                      <  2:317672/138‑1 (MQ=11)
                                                                   aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                      <  1:29014/141‑1 (MQ=11)
                                                                    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                     <  2:89365/141‑1 (MQ=255)
                                                                     tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                    <  1:518628/141‑1 (MQ=18)
                                                                     tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                    <  2:813856/141‑1 (MQ=21)
                                                                     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                    <  1:877550/141‑1 (MQ=11)
                                                                     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                    <  1:861230/141‑1 (MQ=11)
                                                                     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTCCCTGCCGGTCGTGTACACCACGTTCTCTTaa                                                                    <  2:910536/141‑1 (MQ=11)
                                                                        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                 >  1:36791/5‑141 (MQ=255)
                                                                        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGCGGATCATAGGATGATGAACGTTTATACCACGGTCTCTTAatat                                                                 >  1:726337/5‑141 (MQ=11)
                                                                        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCATGAACCCTACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                 >  1:696382/5‑141 (MQ=11)
                                                                        gATGTACGGCTCATCAATACGGCATTTGTAATAAGCATTTTTATGCTTTTTCTTGCAAATATGATAAGAAATTGGGGCATATTCTTGAACCAAAAGGAAGACCGTTGAATGATGAACGTTTACACCACGGTCCGTTAatat                                                                 >  2:444244/1‑141 (MQ=11)
                                                                            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                             >  1:619749/1‑141 (MQ=21)
                                                                              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                           >  1:449421/1‑141 (MQ=255)
                                                                                 ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGTTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                        >  1:502082/1‑141 (MQ=255)
                                                                                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                      >  2:341922/1‑141 (MQ=255)
                                                                                     tcaATACGCCATTTGTAATAAGCTTTTTTTTGTTTTTTCTTCCAAAATTCATCTAACATTGGAGAGTATTCTTAAACCCAACGGTAGGTCGTTGAATGATTACTGTTTGCACCACGTTCTCTTAATATTTCAGATATATCa                                                    >  2:292067/1‑141 (MQ=2)
                                                                                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATAGAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACACCGTTCTCGTAATATTGCCGATATATCa                                                    >  1:31500/1‑141 (MQ=11)
                                                                                      caATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                   <  2:56714/141‑1 (MQ=255)
                                                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAca                                                  >  2:191409/1‑140 (MQ=255)
                                                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAATGGGGCTCACTCTTGAATCTATCGGTATTTCGGTGAATTATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATAACg                                                  >  1:652073/1‑141 (MQ=11)
                                                                                        aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACTTTGCCTATTTAATATTTCAGATATATCACGa                                                 <  1:935937/141‑1 (MQ=14)
                                                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGaa                                                >  1:71145/1‑140 (MQ=25)
                                                                                          aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTATTATTTCAGATATATCACGATa                                               >  2:684247/1‑141 (MQ=255)
                                                                                          aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATACTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                               <  1:635628/141‑1 (MQ=21)
                                                                                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCATTACTATTTCAGATATATCACGATaa                                              >  2:763279/1‑141 (MQ=255)
                                                                                              cATTTGTAATAGGCTTTTTTATGCTTCTTCTTGCCAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACCCCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                           <  2:514156/141‑1 (MQ=14)
                                                                                              cATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACTACGTTCCCTTAATATTTCAGATTTATCACGATAACTc                                           >  2:554106/1‑141 (MQ=11)
                                                                                               ttttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                          <  2:499867/140‑1 (MQ=255)
                                                                                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                          >  2:672254/1‑141 (MQ=255)
                                                                                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                         <  2:403747/141‑1 (MQ=255)
                                                                                                 ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                        <  1:246687/141‑1 (MQ=21)
                                                                                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCACATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                       <  1:433742/141‑1 (MQ=11)
                                                                                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCAGATATATCAGGATAACTCAATgca                                     >  2:385080/1‑139 (MQ=255)
                                                                                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                     <  2:627971/141‑3 (MQ=17)
                                                                                                     aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                    >  1:758683/1‑138 (MQ=11)
                                                                                                                                          |                                                                                                                                         
CAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAC  >  CP000730/36240‑36515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: