BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	90.3%	L58L (TTG→TTA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	90.3%	2044.9 / 195.5	1012	L58L (TTG→TTA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (53/45); new base T (446/456); total (507/505)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 1012 total aligned reads displayed.

CAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAC  >  CP000730/36240‑36515

 aaCCAAATATCCAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                                                                                        <  1:269629/141‑1 (MQ=255)

  aCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                       <  2:361056/141‑1 (MQ=255)

  aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                       >  2:164493/1‑141 (MQ=38)

   ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAATTTTGCTATAAAAt                                                                                                                                      >  2:73029/1‑141 (MQ=11)

   ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                      >  1:198743/1‑141 (MQ=255)

      aaTAACTAATGTATGTACCTCTGTATAAATGGCCCGCTATAACTATCTCCATTTTCCTTTTAATTTGATATACGTCACATCACTACGGCTTTTGTAATAAAATTTTTTATGCTTTTTCTTCCAAATTTAACATCAAATTgg                                                                                                                                   >  2:258124/1‑141 (MQ=2)

        tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATATAAAATTgggg                                                                                                                                 <  1:560419/141‑1 (MQ=255)

        tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATAAGACATTTGTAATAAGCTTTTTTGTGCTTTTTCTTCCTAATTTGATATAAAATTtggg                                                                                                                                 >  1:786191/1‑141 (MQ=11)

          tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                                                                               <  1:944137/141‑1 (MQ=255)

           cTAATGTTTGTCCCTTTGGATCAATGGCACGATATGAATAGCTCCATTTTCCTTTTATTTTGATGGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                              <  1:540774/141‑1 (MQ=11)

           cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                              <  1:166398/141‑1 (MQ=255)

           cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCat                                                                                                                              <  2:479050/141‑1 (MQ=255)

              atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATAtt                                                                                                                           <  2:208326/141‑1 (MQ=255)

               tgtatgtCCCTCTGTATCAATGGCACGATATTCATCGCCCTATCTGCCGTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCATATAAAATTGGGGCATATTc                                                                                                                          <  1:489102/141‑1 (MQ=11)

               tgtatgtCCCTCTGCATCAATGGCAAGAAATAAATAGCTCCATTGTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTc                                                                                                                          >  2:839373/1‑141 (MQ=255)

                gtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                         <  2:373548/141‑1 (MQ=37)

                  atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATATTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                       <  1:668002/141‑1 (MQ=11)

                   tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTTATCAATACGCCTTTTGTAATAAGATTTTTTATGCTTTTTCTTCCAAATTTGATCGAAAAGTGGTGTATATTCttca                                                                                                                      >  1:102979/1‑139 (MQ=14)

                     tcctttggCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                    <  2:650353/134‑1 (MQ=255)

                     tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCGGAAGAATTTGGGGCATATTCTTGAAc                                                                                                                    >  1:122449/1‑141 (MQ=11)

                     tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                    >  1:800359/1‑141 (MQ=21)

                        ctctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACATCTCATCCATACGCCATTTGTATTAAGCTTTTTTATGGTTTTTCTTGCAAATTTAATAAAAAATTGGGGCTTTTTTGTGAAACCa                                                                                                                 >  1:228445/1‑141 (MQ=11)

                          ctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                                                                               >  1:589307/1‑141 (MQ=21)

                          ctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTAGAACGCAAc                                                                                                               >  1:733855/1‑141 (MQ=255)

                            gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTGCTTCCAAATTTGATATAGAATTGGGGCATATTCTTGAACCCAACgg                                                                                                             >  1:144995/3‑141 (MQ=11)

                            gtaTCAATGGCACGATATAAATAGCTCCATTTCCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCATATTCTTGAACCCAACgg                                                                                                             >  1:387870/3‑141 (MQ=11)

                             taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                            >  1:896423/2‑141 (MQ=255)

                             cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCTCATAAATACGCCATTTGTAATACGCTTTTTTATGCTTTTTCTTGCAAATTTGATCTAAAATTGGGGCATATTTTTGAACCCTACGGt                                                                                                            >  1:917619/1‑141 (MQ=11)

                             cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCTTATTCTTGAACCCAACGGt                                                                                                            >  1:340485/1‑141 (MQ=255)

                              aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                                                                                           >  2:749500/1‑141 (MQ=255)

                              aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATGGGGGCATATTCTTGAACCCAACGGtc                                                                                                           <  2:586347/141‑2 (MQ=16)

                               tCAATGGCCCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACATCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                          <  2:608365/141‑1 (MQ=11)

                               tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAg                                                                                                          >  1:60742/1‑141 (MQ=255)

                                cAATGGCACGATAAAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                         <  1:58008/141‑1 (MQ=255)

                                  aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatc                                                                                                       <  1:841864/141‑3 (MQ=16)

                                     gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                    >  1:309778/1‑141 (MQ=255)

                                       aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                  <  1:831396/141‑1 (MQ=255)

                                       aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                  >  1:182004/1‑141 (MQ=255)

                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGGTTTTACTTCTAACTTTGATACAAAATTGTGTCATATTCTTGAACCCACCAGTAGACCGTTGaa                                                                                                 >  2:417290/1‑141 (MQ=14)

                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTTGACCGTTGaa                                                                                                 >  2:701511/1‑141 (MQ=21)

                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                 >  2:827536/1‑141 (MQ=255)

                                         gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                                >  2:110528/1‑141 (MQ=255)

                                         gATATAAATAGCTCCATTTTCCTTTTAGTTGGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGTAAATTTGCTATAAAATTGGGGCATATTCTTGAACCCAACGGCAGCTCGTTgtaa                                                                                                >  2:276805/1‑138 (MQ=11)

                                           tataAATAGGTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                              <  2:180998/141‑1 (MQ=255)

                                            ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                                                                             >  2:132047/1‑141 (MQ=255)

                                              aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACACAACGGTAGATCGTTGCatggtta                                                                                           >  1:210470/1‑137 (MQ=11)

                                               aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                                                                                          >  1:823499/1‑141 (MQ=21)

                                                 tAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                        <  2:793171/141‑1 (MQ=255)

                                                   gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                      <  1:780858/141‑1 (MQ=255)

                                                      ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                                                                                   <  2:875549/141‑1 (MQ=18)

                                                        agttttCTTTTATTTTGATATACGTTTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                 <  2:151994/139‑1 (MQ=11)

                                                        aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                 <  2:858799/141‑1 (MQ=255)

                                                        aTTTTCCTTTTATTTGGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                 <  2:225930/141‑1 (MQ=18)

                                                         ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                <  1:403673/141‑1 (MQ=21)

                                                         ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                <  2:465143/141‑1 (MQ=18)

                                                         ttttCCTTTTATTTTGATATACTTTTCATCAATACGCTATTTGTAATACGCTTTTTTATTCTTTTTCTTCCAAATTTGATATTAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                <  1:358754/141‑1 (MQ=11)

                                                         ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                >  2:23921/1‑141 (MQ=255)

                                                         ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                <  1:466321/141‑1 (MQ=255)

                                                         ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTCTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGCTGAATGATGAACGTTTAcacc                                                                                >  2:5326/1‑141 (MQ=11)

                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                            >  1:812188/1‑141 (MQ=40)

                                                              cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                           <  2:932229/141‑1 (MQ=255)

                                                              cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                           <  1:68107/141‑1 (MQ=255)

                                                               ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTc                                                                          <  2:779329/141‑1 (MQ=18)

                                                                  tagtttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATCCTTTTAGTCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                       <  1:413449/138‑4 (MQ=11)

                                                                   atcttGATATACGTCTCATCAATACGCCATTTGTAATAATCGTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                      <  2:317672/138‑1 (MQ=11)

                                                                   aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                      <  1:29014/141‑1 (MQ=11)

                                                                    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                     <  2:89365/141‑1 (MQ=255)

                                                                     tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                    <  1:518628/141‑1 (MQ=18)

                                                                     tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                    <  2:813856/141‑1 (MQ=21)

                                                                     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                    <  1:877550/141‑1 (MQ=11)

                                                                     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                    <  1:861230/141‑1 (MQ=11)

                                                                     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTCCCTGCCGGTCGTGTACACCACGTTCTCTTaa                                                                    <  2:910536/141‑1 (MQ=11)

                                                                        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                 >  1:36791/5‑141 (MQ=255)

                                                                        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGCGGATCATAGGATGATGAACGTTTATACCACGGTCTCTTAatat                                                                 >  1:726337/5‑141 (MQ=11)

                                                                        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCATGAACCCTACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                 >  1:696382/5‑141 (MQ=11)

                                                                        gATGTACGGCTCATCAATACGGCATTTGTAATAAGCATTTTTATGCTTTTTCTTGCAAATATGATAAGAAATTGGGGCATATTCTTGAACCAAAAGGAAGACCGTTGAATGATGAACGTTTACACCACGGTCCGTTAatat                                                                 >  2:444244/1‑141 (MQ=11)

                                                                            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                             >  1:619749/1‑141 (MQ=21)

                                                                              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                           >  1:449421/1‑141 (MQ=255)

                                                                                 ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGTTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                        >  1:502082/1‑141 (MQ=255)

                                                                                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                      >  2:341922/1‑141 (MQ=255)

                                                                                     tcaATACGCCATTTGTAATAAGCTTTTTTTTGTTTTTTCTTCCAAAATTCATCTAACATTGGAGAGTATTCTTAAACCCAACGGTAGGTCGTTGAATGATTACTGTTTGCACCACGTTCTCTTAATATTTCAGATATATCa                                                    >  2:292067/1‑141 (MQ=2)

                                                                                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATAGAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACACCGTTCTCGTAATATTGCCGATATATCa                                                    >  1:31500/1‑141 (MQ=11)

                                                                                      caATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                   <  2:56714/141‑1 (MQ=255)

                                                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAca                                                  >  2:191409/1‑140 (MQ=255)

                                                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAATGGGGCTCACTCTTGAATCTATCGGTATTTCGGTGAATTATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATAACg                                                  >  1:652073/1‑141 (MQ=11)

                                                                                        aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACTTTGCCTATTTAATATTTCAGATATATCACGa                                                 <  1:935937/141‑1 (MQ=14)

                                                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGaa                                                >  1:71145/1‑140 (MQ=25)

                                                                                          aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTATTATTTCAGATATATCACGATa                                               >  2:684247/1‑141 (MQ=255)

                                                                                          aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATACTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                               <  1:635628/141‑1 (MQ=21)

                                                                                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCATTACTATTTCAGATATATCACGATaa                                              >  2:763279/1‑141 (MQ=255)

                                                                                              cATTTGTAATAGGCTTTTTTATGCTTCTTCTTGCCAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACCCCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                           <  2:514156/141‑1 (MQ=14)

                                                                                              cATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACTACGTTCCCTTAATATTTCAGATTTATCACGATAACTc                                           >  2:554106/1‑141 (MQ=11)

                                                                                               ttttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                          <  2:499867/140‑1 (MQ=255)

                                                                                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                          >  2:672254/1‑141 (MQ=255)

                                                                                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                         <  2:403747/141‑1 (MQ=255)

                                                                                                 ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                        <  1:246687/141‑1 (MQ=21)

                                                                                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCACATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                       <  1:433742/141‑1 (MQ=11)

                                                                                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCAGATATATCAGGATAACTCAATgca                                     >  2:385080/1‑139 (MQ=255)

                                                                                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                     <  2:627971/141‑3 (MQ=17)

                                                                                                     aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgcat                                    >  1:758683/1‑138 (MQ=11)

CAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAC  >  CP000730/36240‑36515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`