Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,444 C→T 50.9% R36R (AGG→AGA USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4440CT50.9% 33.0 / inf 421R36R (AGG→AGAUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (109/96);  new base T (104/110);  total (214/207)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.12e-01

Only 100 of 435 total aligned reads displayed.

TTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTAT  >  CP000730/36304‑36584
                                                                                                                                              |                                                                                                                                            
  tATTTTGATTTACGTCTCATTCATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                                                            <  2:157611/141‑1 (MQ=255)
  tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                                                            <  2:19411/141‑1 (MQ=40)
   tttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                           <  2:613062/140‑1 (MQ=18)
   atttttctatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                           <  2:474334/132‑1 (MQ=11)
   atcttGATATACGTCTCATCAATACGCCATTTGTAATAATCGTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                           <  2:317672/138‑1 (MQ=11)
   aTTTTGATGTACGTCTCCTCAATACGCCATTTGTAATAAGATTTTTTCTGGAGCGTGGCCATTTTACTATGTGACATTGGGGCCTATTATATAGCCCTTCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                           <  2:664681/141‑1 (MQ=2)
   aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                                                           <  1:945737/141‑1 (MQ=18)
   aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                           <  1:60819/141‑1 (MQ=255)
   aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                                                           <  1:808264/141‑1 (MQ=255)
    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                                                          <  1:533616/141‑1 (MQ=255)
    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                                                          <  1:365470/141‑1 (MQ=255)
    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTGTCACCACGTTCTCTTa                                                                                                                                          <  1:471147/141‑1 (MQ=11)
     tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                                                         <  1:518628/141‑1 (MQ=18)
     tttGATATACGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCCTTAATGGTGGAACTTTTACACCACATTCTCTTaa                                                                                                                                         <  1:692991/141‑1 (MQ=11)
     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                         <  2:384941/141‑1 (MQ=11)
     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                         <  1:840460/141‑1 (MQ=11)
       tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTTTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                                                                                       >  1:497421/1‑141 (MQ=11)
        gttGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                                                      <  1:714682/139‑1 (MQ=18)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACGCCACGTTCTCTTAatat                                                                                                                                      >  1:567388/5‑141 (MQ=11)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                      >  1:592188/5‑141 (MQ=255)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                                                      >  1:678116/5‑141 (MQ=255)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACAACGTTCTCTTActat                                                                                                                                      >  2:483229/5‑141 (MQ=11)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGCGGATCATAGGATGATGAACGTTTATACCACGGTCTCTTAatat                                                                                                                                      >  1:726337/5‑141 (MQ=11)
        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                                                      >  1:642778/1‑141 (MQ=25)
          tatACGTCTCATCAATCCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                                                                                    <  1:40809/139‑1 (MQ=11)
          tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                                                                                    <  1:586102/139‑1 (MQ=255)
            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                                                  >  1:619749/1‑141 (MQ=21)
            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                                                  <  2:11951/141‑1 (MQ=21)
            aaCGTCTCATCAATCTGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                                                  <  1:506394/140‑1 (MQ=11)
             aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCaa                                                                                                                                 >  2:439776/1‑140 (MQ=21)
              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAGTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACCCCACGTTCTCTTAATATTTCAGa                                                                                                                                >  1:704833/1‑141 (MQ=11)
              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                                                                                >  2:874259/1‑141 (MQ=255)
               gTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAATGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                                                               <  1:632752/141‑1 (MQ=255)
               gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                               <  1:111125/141‑1 (MQ=255)
               gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                               >  2:553276/1‑141 (MQ=255)
                tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTATTTTTGATATAAAATTAGGACATATTCTTGAACCCAATGGTAGATTGTTGAATGATGACCGTTTACAGCACGTTCTCTTAATATTTCAGatt                                                                                                                              >  2:531919/1‑140 (MQ=11)
                tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCACTTAATCTTTCAGatt                                                                                                                              >  2:896683/1‑140 (MQ=255)
                  tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGATTGATATGGGAGACCGTTGAATGATGACCGTTTACACCACGTTCCCTTAATATTTCAGatata                                                                                                                            >  1:217618/1‑141 (MQ=11)
                  ccatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                                                                                            <  1:3140/140‑1 (MQ=255)
                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                         <  2:757237/127‑1 (MQ=255)
                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                                                         >  1:757237/1‑127 (MQ=255)
                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                                                           >  2:341922/1‑141 (MQ=255)
                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatatt                                                                                                                          >  2:625123/1‑140 (MQ=255)
                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                          >  2:150019/1‑141 (MQ=255)
                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                          >  2:370814/1‑141 (MQ=255)
                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTTGATCGGTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatctataa                                                                                                                         >  2:247204/1‑139 (MQ=11)
                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATGTTTCAGATATATCa                                                                                                                         >  1:24390/1‑141 (MQ=255)
                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                                                         >  1:783522/1‑141 (MQ=255)
                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCACATATATCa                                                                                                                         >  2:791929/1‑141 (MQ=255)
                      caATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                                                        <  2:56714/141‑1 (MQ=255)
                      caATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATTTGATATAAAATTATACGATATTCTTTAGCCGAATGGTAGATGGTTTCTTGCTAAACGTTTACACCACATTCTCTTAATtttttgaatttatttt                                                                                                                        >  2:580693/1‑129 (MQ=2)
                        aTACGCCATTTGTAATACGCTCCAATAGTCTGTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                      >  2:910567/1‑141 (MQ=11)
                        aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                      >  1:415653/1‑141 (MQ=255)
                        aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACTTTGCCTATTTAATATTTCAGATATATCACGa                                                                                                                      <  1:935937/141‑1 (MQ=14)
                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATGCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGCTGAATGATGAACGGTTATGCCACGCTCCCTTAATATTTCAGagatatagagac                                                                                                                     >  1:387371/1‑135 (MQ=14)
                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                                                     >  2:852918/1‑141 (MQ=255)
                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATAAATCACGAt                                                                                                                     >  2:398530/1‑141 (MQ=255)
                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTGCACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                                                     >  1:173752/1‑141 (MQ=21)
                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                   >  2:677014/1‑140 (MQ=255)
                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCATTACTATTTCAGATATATCACGATaa                                                                                                                   >  2:763279/1‑141 (MQ=255)
                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                   >  1:767594/1‑141 (MQ=40)
                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATGTAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                 >  2:514524/1‑141 (MQ=25)
                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                 >  2:828577/1‑141 (MQ=255)
                              cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                <  1:340647/141‑1 (MQ=11)
                              cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                >  1:66601/1‑141 (MQ=21)
                              cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                <  1:794981/141‑1 (MQ=21)
                               ttttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                                                               <  2:499867/140‑1 (MQ=255)
                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATACATCACGATAGCTCa                                                                                                               >  2:715367/1‑141 (MQ=11)
                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                               <  1:828474/141‑1 (MQ=21)
                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                               <  2:740273/141‑1 (MQ=18)
                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                               <  1:904008/141‑1 (MQ=18)
                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                              >  2:35773/1‑141 (MQ=11)
                                tttGTAATAAGCGTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTTTTGAACCCAACGGTAGACCGTTGAATGATGTACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                              <  2:941906/141‑1 (MQ=11)
                                 ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                             <  2:297182/141‑1 (MQ=11)
                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaagg                                                                                                            >  1:846773/1‑139 (MQ=11)
                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                            <  2:223622/141‑1 (MQ=255)
                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                            >  1:454022/1‑141 (MQ=255)
                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAAGATTTCAGATATATCACGATAGCTCAATg                                                                                                            <  1:298819/141‑1 (MQ=11)
                                   gTAATAAGGTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                           <  2:643960/141‑2 (MQ=17)
                                   gTAATAAGCTTTTTTATGCTTTTGCTTCCAAATTTGATATAAAATGAGGGCATGTTCTTGAACCCAACGGTTGCTCGATCAATGATGAAAGCTTACACCACGATCTCTTACTCTTTAAGATATATCACGATAGCTCactcc                                                                                                           >  2:185216/1‑137 (MQ=2)
                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                                                          >  1:429159/1‑141 (MQ=255)
                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTCGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                          <  1:377304/141‑3 (MQ=12)
                                                  aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                            >  1:868803/1‑141 (MQ=25)
                                                  aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                            >  1:184727/1‑141 (MQ=35)
                                                        tttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGccgtt                                                                                      <  1:544293/141‑4 (MQ=25)
                                                                        aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATGTCTCACGATAACTCAATGTATATCTTAGATAGTAGCCAGCGGCTACAGTGATaaa                                                                       >  1:26528/1‑140 (MQ=255)
                                                                              aaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCtt                                                                 <  1:883063/141‑1 (MQ=32)
                                                                                 tGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCCCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGtt                                                              <  2:288636/141‑1 (MQ=17)
                                                                                    ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                                                           >  2:256505/1‑141 (MQ=32)
                                                                                     gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                          >  2:880897/1‑141 (MQ=32)
                                                                                       atatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGCGATAACATCCTTGTTAAATTg                                                        <  1:286313/141‑1 (MQ=17)
                                                                                        tatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGt                                                       <  2:27748/141‑1 (MQ=32)
                                                                                              tGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATCTTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTatat                                                 >  1:153761/1‑141 (MQ=17)
                                                                                               tgactcAATGGTAGAACCTAGAAATATGACCGTTCACACCACGTTCTCTTAACATTTCAGATATATCACGATAGATAAGTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATc                                                <  1:132173/136‑1 (MQ=11)
                                                                                                 aCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTg                                              >  1:819449/1‑141 (MQ=32)
                                                                                                           aGACCGTTGAATGAAGAACGTTTACACCACGTTCCCTTAATATTTCAGATATAACACGGTAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAGTGTTTATATCTGAAATAGTTCa                                    <  1:659888/141‑1 (MQ=255)
                                                                                                              tcGTTGAAGGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAc                                 <  2:701723/140‑1 (MQ=12)
                                                                                                                     atgattAACGTGTACACCAGGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                          <  2:410920/141‑1 (MQ=12)
                                                                                                                            aaCTTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttt                    <  2:80749/141‑1 (MQ=17)
                                                                                                                                   acacCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaa             >  1:858728/1‑141 (MQ=32)
                                                                                                                                              |                                                                                                                                            
TTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTAT  >  CP000730/36304‑36584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: