Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 37.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT37.7% 201.2 / 129.3 147intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (37/53);  new base T (27/28);  total (65/82)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

Only 100 of 147 total aligned reads displayed.

GAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAAT  >  CP000730/76041‑76315
                                                                                                                                           |                                                                                                                                       
gAATTTAATATAACTATTATAACTGCACCGGTATCACTTCGAACAGCTAACCAAAGTGTTAGTAATCCAGCGAATACTCACACAAAGGCAATTAATTTAATAATTATTGCAAAATATAGGCTCTCCTGCCAAGTTGAGTCt                                                                                                                                        >  2:433384/1‑141 (MQ=255)
      aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTATTAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                  <  2:611513/141‑1 (MQ=255)
          taACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATGTATAGTATAAt                                                                                                                              >  2:15717/1‑141 (MQ=255)
                     gctgcACCTGTATCACTTAGAACTGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAACGGCAATTAATTTAATAATTATAGCAAAGTATAGGTTCTGTTGCAAAGCTGAATCTACAGTATAATTTTTAccacaa                                                                                                                   >  2:257010/1‑136 (MQ=255)
                     gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATACTTATAGCAAACTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTACaaaaaa                                                                                                                   >  2:5851/1‑141 (MQ=255)
                     gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                   >  2:818384/1‑141 (MQ=255)
                     gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAATATAATTTTAACaaaaa                                                                                                                   >  1:282267/1‑141 (MQ=255)
                       tgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCAATGGCAACAATTTAACACAAAgg                                                                                                                 >  2:313842/1‑141 (MQ=255)
                         cACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTAAACAAAAAGGAg                                                                                                               <  2:652946/141‑1 (MQ=255)
                           ccTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATGGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtc                                                                                                             <  1:27758/141‑1 (MQ=255)
                            cTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtct                                                                                                            <  1:347971/141‑1 (MQ=255)
                             tGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATTTTTGCCTTATTTCAATGATGAATTTATAGTATAAGTTTAACAAAAAGGAGtctt                                                                                                           >  1:922737/1‑141 (MQ=255)
                                 tCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGt                                                                                                       <  1:182858/141‑1 (MQ=255)
                                 tCACATAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACGAAAAGGAGTCTTCTGt                                                                                                       <  1:468624/141‑1 (MQ=255)
                                    cTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                    <  1:248353/141‑1 (MQ=255)
                                      aaaaatagatAAGCAAAGTTTTAGTAATCCAGGGAATTCTAACACAAAGGCAATTAATTTAATAATTATAGAAAAATATAGGTTCTGTTGCAAAGTTGAATCTTTAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGaa                                                                                                  <  2:942527/132‑1 (MQ=25)
                                            aGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAttt                                                                                            <  1:73582/141‑1 (MQ=255)
                                            aGCTAACCAAAGTGTTAGGAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAttt                                                                                            <  2:492116/141‑1 (MQ=255)
                                              cgaaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATCATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATCAACTATTTCa                                                                                          <  1:684484/139‑1 (MQ=255)
                                                 aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTTTATGAACTATTTCAGat                                                                                       >  2:633654/1‑141 (MQ=255)
                                                    aaaGTGTTAGTAATCCAGGGAAAACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAtata                                                                                    <  1:170568/141‑1 (MQ=255)
                                                         gtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACaa                                                                               <  1:651284/141‑1 (MQ=255)
                                                           tatgaaTCCAGGGAATACGAACACAAAGCCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAATTTGAATCTATAGTATAATTTTAACAAAAATGAGTCTTCTGTATGAACTATTTCAGATATAAACAAtt                                                                             <  1:231056/137‑1 (MQ=21)
                                                           tAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAtt                                                                             <  1:317471/141‑1 (MQ=255)
                                                                 tCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACaa                                                                       >  2:4545/1‑141 (MQ=255)
                                                                   cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAgg                                                                     >  1:56431/1‑141 (MQ=255)
                                                                     gggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                   >  1:700082/1‑141 (MQ=255)
                                                                      ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                  >  2:928255/1‑141 (MQ=255)
                                                                       gAATACAAAAACAAAGGAAAATAATTTAATAATTATAGAAAAATAAAGGCTCAGATGCAAAGTCGAATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGt                                                                 <  2:840240/141‑1 (MQ=17)
                                                                               acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                         <  1:797043/141‑1 (MQ=255)
                                                                                cacaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGt                                                        <  1:103436/141‑1 (MQ=255)
                                                                                 acaAAGGCAATTAATTTAATAATTATAGCAACATGTGTAGTCTGCTGCCCCATGGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                       >  1:452626/1‑141 (MQ=14)
                                                                                 acaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                       <  1:250573/141‑1 (MQ=255)
                                                                                    aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATTTTATCACTGTAGcc                                                    <  1:615595/141‑1 (MQ=255)
                                                                                    aaGGCAAATAATTTAATAATTATAGCAAAATATAGGTTCCGTTGCAAATTTGAATCTATAGTATAATATTAAAAAACACACGAATAATATATAATATTTTTCTGATATAAACAATTTAACAAGGAGTTTATCACTGTGGcc                                                    <  2:388966/141‑1 (MQ=16)
                                                                                     aGGCAATTAATTTAATAATTATAGGAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAACGATGTTATCACTGTAGCCg                                                   >  1:602303/1‑141 (MQ=255)
                                                                                      ggCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGt                                                  >  1:117265/1‑141 (MQ=255)
                                                                                      ggCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGACGt                                                  >  2:862870/1‑141 (MQ=255)
                                                                                        cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTGTAATGTGAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTg                                                >  2:288168/1‑141 (MQ=255)
                                                                                        cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAGCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGtgaaccgtg                                                >  2:643247/1‑133 (MQ=255)
                                                                                         aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                               <  1:91458/141‑1 (MQ=255)
                                                                                         aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                               <  1:818384/141‑1 (MQ=255)
                                                                                         aattaattTAAAAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTTAATCAATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                               <  2:127178/141‑1 (MQ=255)
                                                                                             aatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctac                                           >  1:849075/1‑141 (MQ=255)
                                                                                                   ataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTa                                     >  1:502288/1‑141 (MQ=255)
                                                                                                     aatGATACCAAAATATAGGTTCTGTTGCAAAGTTGAATATATAGGATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTGTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAg                                   <  1:433384/141‑1 (MQ=14)
                                                                                                         ataGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGatat                               <  1:3307/141‑1 (MQ=255)
                                                                                                           aGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGc                             <  2:545266/141‑1 (MQ=255)
                                                                                                           aGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGc                             <  2:63043/141‑1 (MQ=255)
                                                                                                             cagttcggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           >  2:409761/9‑141 (MQ=255)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACCATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           <  1:53548/133‑1 (MQ=11)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           <  1:566155/133‑1 (MQ=11)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           >  2:88481/9‑141 (MQ=11)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           <  1:460335/133‑1 (MQ=11)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                           >  2:210470/9‑141 (MQ=11)
                                                                                                             agtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                           <  1:670086/133‑1 (MQ=11)
                                                                                                             agtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                           <  1:305690/133‑1 (MQ=11)
                                                                                                              gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAGGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATTTAAGATATGCAtt                          >  2:884186/8‑141 (MQ=1)
                                                                                                              gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTAAAAAAAAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                          <  2:839408/134‑1 (MQ=11)
                                                                                                              gtaaatgaGGTTCTGTAGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                          >  1:484123/8‑141 (MQ=1)
                                                                                                              agttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtt                          >  2:908424/8‑141 (MQ=255)
                                                                                                               taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                         <  2:715490/135‑1 (MQ=255)
                                                                                                               taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                         >  2:329054/7‑141 (MQ=0)
                                                                                                               taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                         <  1:904684/135‑1 (MQ=255)
                                                                                                               aaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                         <  1:854619/141‑1 (MQ=255)
                                                                                                                aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                        >  2:733855/6‑141 (MQ=0)
                                                                                                                aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                        <  1:151528/136‑1 (MQ=9)
                                                                                                                aaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                        >  1:541048/1‑141 (MQ=40)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       <  1:136349/137‑1 (MQ=2)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       <  1:745401/137‑1 (MQ=9)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       <  2:549448/137‑1 (MQ=9)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTAGCGAAGATATGCATTGAg                       >  1:346611/5‑141 (MQ=11)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTACGATATAAACAAGTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       >  1:218683/5‑141 (MQ=1)
                                                                                                                 taggaGGTTCTGTTGCAAAGCTTAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                       <  2:408529/137‑1 (MQ=11)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCGCTGTAGCTGTTGGCTACTATCTAAGATATGCATTggg                       >  2:310058/5‑139 (MQ=11)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                       <  1:378279/137‑1 (MQ=2)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                       >  2:296782/5‑141 (MQ=0)
                                                                                                                  tataGGTTTTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGt                      <  1:794059/141‑1 (MQ=38)
                                                                                                                  aggaGGTGCTGTGGCAAAGTTGAATTTATAATATAATTTTCACAAAAAGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCTCTGTAGCCGTTGGCTACTATCTTAGTTATGCATTGAgc                      <  2:415360/138‑2 (MQ=11)
                                                                                                                   tgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                     <  1:223451/139‑1 (MQ=9)
                                                                                                                   tgaGGTTCTGGTGCAAAGTTGAATTTATATTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                     <  2:91987/139‑1 (MQ=11)
                                                                                                                   ggaGGTTCTGTTGCCAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     >  2:574199/3‑139 (MQ=0)
                                                                                                                   ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     >  1:102642/3‑139 (MQ=2)
                                                                                                                   ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     >  2:705057/3‑139 (MQ=2)
                                                                                                                   ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTGCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     <  1:26698/139‑3 (MQ=255)
                                                                                                                   ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTACTGTATGAACTATTTCAGATCTAAACAATTTAACAAGGATGTCATCACTTTAGCCGTTGGCTACTATCTAAGATATGCATTGaact                     >  2:395228/3‑138 (MQ=11)
                                                                                                                   ggaGGTTCTATTGCAAAGTTGAATTTATAATATAATAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                     <  2:866317/139‑3 (MQ=11)
                                                                                                                   ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                     <  1:404232/141‑1 (MQ=38)
                                                                                                                   ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGCAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                     <  2:241699/141‑1 (MQ=38)
                                                                                                                    gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                    >  2:781528/2‑141 (MQ=0)
                                                                                                                     aGGTTCTGTTGCAAAGTTGTATTTATAATATAATTTTAACAAAATGGTGTCTTCTGTATGCACTATTTCAGATTTAAACAATTTAACAAGGATGTTATGACTGTAGCGGTTGGTTACTATCTAAGTTTTGCATTGAGCTAt                   >  1:826037/1‑141 (MQ=1)
                                                                                                                     aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                   >  2:725801/1‑141 (MQ=37)
                                                                                                                      ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                  >  1:373346/1‑141 (MQ=34)
                                                                                                                          tgcttGCGAAGTATAATCTATAATATAATATTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGa              <  2:552892/138‑1 (MQ=11)
                                                                                                                          cggTTGGAAAGTTGAAGCTATAGTATAAGTTTAACAAAAAGGAGTTTTCTGTATGAACTATTTCAGATATAATCAATTGAACAAGGATGTTATCACTGTAGCCGTTGGCTAGTATTTAAGATAGGCATTGAGTTATCGTGa              <  2:341323/139‑1 (MQ=11)
                                                                                                                            ggtGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata            <  2:484841/139‑1 (MQ=25)
                                                                                                                               gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat         >  1:235829/1‑141 (MQ=35)
                                                                                                                                cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc        >  2:819530/1‑141 (MQ=35)
                                                                                                                                   aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa     <  1:284297/141‑1 (MQ=35)
                                                                                                                                    gTTGAATCTATAGTATAATTTTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa    <  2:23443/141‑1 (MQ=25)
                                                                                                                                           |                                                                                                                                       
GAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAAT  >  CP000730/76041‑76315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: