Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,858 T→A 38.3% intergenic (+89/+19) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8580TA38.3% 203.7 / 155.7 150intergenic (+89/+19)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (48/41);  new base A (21/35);  total (72/78)
Fisher's exact test for biased strand distribution p-value = 6.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 151 total aligned reads displayed.

CAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCT  >  CP000730/35718‑35992
                                                                                                                                            |                                                                                                                                      
cAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAtt                                                                                                                                        <  1:309789/141‑1 (MQ=255)
   aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                                                                     >  2:65946/1‑141 (MQ=255)
        ttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTGACCACTAATTTATCATGt                                                                                                                                >  1:706363/1‑141 (MQ=255)
        ttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGt                                                                                                                                >  1:797042/1‑141 (MQ=255)
         tAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAACAATATATAGCTAACCACTAATTTATCATGTc                                                                                                                               <  2:909844/141‑1 (MQ=255)
              tAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGGTTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                                                          <  2:617546/141‑1 (MQ=255)
              tAGAAGCTATTCAGTATAAAGTTTTTGGAGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                                                          <  2:14991/141‑1 (MQ=255)
              tAGAAGCTATTCAGCATAAAGTTTGTGGCGAGAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                                                          <  1:442742/141‑1 (MQ=255)
                 aaGCTATTTGTTATAAAGTTTTGGGTGAAAAATTATTACTTGAACAAGAGCTAAAATAAAATTGTTATCTAATAAAAATAGAAGGTGCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                                                       <  1:192798/141‑1 (MQ=25)
                  aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                      >  1:559189/1‑141 (MQ=255)
                   gCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCt                                                                                                                     >  1:256256/1‑141 (MQ=255)
                      aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                                                                  >  2:490116/1‑141 (MQ=255)
                      aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                                                                  >  2:341992/1‑141 (MQ=255)
                      aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCACGTCAGTGTTCGCTaaa                                                                                                                  >  2:87173/1‑141 (MQ=255)
                        tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                                                                >  1:251862/1‑141 (MQ=255)
                            tataAAGTTTTTGGCGAAAAATGATTACCTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTCCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                            <  1:654757/141‑1 (MQ=255)
                             ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTa                                                                                                           >  1:910446/1‑141 (MQ=255)
                             ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTa                                                                                                           >  1:757946/1‑141 (MQ=255)
                                   tttttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTGAGAAAAAATCGCTAACCGCTAATGTATCATGTCCGTGGTAGCGTAACTTGCTGGCatga                                                                                                     >  1:806520/1‑141 (MQ=255)
                                     tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                                   >  2:778364/1‑141 (MQ=255)
                                     gttGGCGAACCATGACTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                                   >  2:914591/2‑141 (MQ=255)
                                      ttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGc                                                                                                  >  2:218511/1‑141 (MQ=255)
                                        ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                                                                >  1:588594/1‑141 (MQ=255)
                                         gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                                                               >  2:870431/1‑141 (MQ=255)
                                               aaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                                                         >  1:157799/1‑141 (MQ=255)
                                                aTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                                                        >  2:630339/1‑141 (MQ=255)
                                                aTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                                                        <  2:892978/141‑1 (MQ=255)
                                                  gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                                                      >  1:69647/1‑141 (MQ=255)
                                                  gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                                                      <  1:329884/141‑1 (MQ=255)
                                                  gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                                                      >  2:893729/1‑141 (MQ=255)
                                                     tACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATg                                                                                   >  2:159782/1‑141 (MQ=255)
                                                          gAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                                                              <  1:127694/141‑1 (MQ=255)
                                                              aaGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATc                                                                          >  1:395350/1‑141 (MQ=255)
                                                               agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                         >  2:474122/1‑141 (MQ=255)
                                                               agagCTAAAATAAAATTGTAATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                         <  2:636929/141‑1 (MQ=255)
                                                               aaagataaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                         <  2:540250/136‑1 (MQ=255)
                                                                gagCTAAAATAAAATTGTGCTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACGAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                                                        >  2:651850/1‑141 (MQ=255)
                                                                 agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                       >  1:457827/1‑141 (MQ=255)
                                                                 agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTACTGTTGCAAAGTAAAAAAATATGGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGCTGCTAATTTCGTGCAATGGAGAAAACCCga                                                                       >  2:777517/1‑140 (MQ=18)
                                                                   cTAAAATAAAATTGTGATCTAATAAAAATACAAGGCTCTGTTGAAAAGTAAAAAAATATAGCTAACCACTAATTTATCACGTCAGTGTAAGCTTCACATACTAGCATGATGCTAGTTTCGTGGCATATCGACAAtacttaa                                                                     >  2:261647/1‑135 (MQ=17)
                                                                    taaaataaaaTTGTGACCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                                                    <  1:814373/141‑1 (MQ=255)
                                                                         caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                             <  1:279027/128‑1 (MQ=11)
                                                                         caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                             <  1:875796/128‑1 (MQ=11)
                                                                         caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                             >  2:351325/14‑141 (MQ=11)
                                                                            aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                          <  1:727922/131‑1 (MQ=11)
                                                                            aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCACGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                          >  2:829369/11‑141 (MQ=11)
                                                                             agattattttaataaAAATGATGGTTCTGGTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGGTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  2:113970/132‑1 (MQ=11)
                                                                             agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  1:529405/132‑1 (MQ=11)
                                                                             agagtattttaaaaaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACAAATCTACCATGTCAGTGTCCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  1:362145/132‑1 (MQ=11)
                                                                              gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        >  1:881506/9‑141 (MQ=11)
                                                                              gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGAcc                                                        >  2:742836/9‑141 (MQ=11)
                                                                               tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  1:319808/141‑1 (MQ=255)
                                                                               tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  1:852574/141‑1 (MQ=255)
                                                                               tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACCTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                         <  1:220618/141‑1 (MQ=255)
                                                                               tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAGTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTTGATCGGGAGAGAc                                                         <  2:437310/141‑1 (MQ=14)
                                                                                ttgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        <  1:506877/140‑1 (MQ=255)
                                                                                gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        <  2:591475/141‑1 (MQ=255)
                                                                                gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        >  1:539373/1‑141 (MQ=255)
                                                                                gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        <  1:136210/141‑1 (MQ=255)
                                                                                gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        >  2:722151/1‑141 (MQ=255)
                                                                                gtgAGCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        <  1:193755/141‑1 (MQ=255)
                                                                                gcgattgaataaCAACGGAAGGTTCTCTTCGAAAGTAACAAAATATAGCTCACCACTAATTCACCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                        <  2:838655/134‑1 (MQ=11)
                                                                                 tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                       <  1:858566/141‑1 (MQ=255)
                                                                                 tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                     <  1:864667/136‑1 (MQ=11)
                                                                                  gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTg                                                      <  1:505047/141‑1 (MQ=255)
                                                                                  gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                      >  2:21678/1‑141 (MQ=255)
                                                                                  attttaataaAAGTGATGGTTCTGTTGCACAGTAAAAAATATCGCTAATAACTCATATATGATGTCAGTGTTAGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAAATCTGAAGAGACCAGCg                                                    >  2:55785/5‑141 (MQ=11)
                                                                                  attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                    <  2:926098/137‑1 (MQ=11)
                                                                                  attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                    <  1:162644/137‑1 (MQ=11)
                                                                                  attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                    <  1:432520/137‑1 (MQ=11)
                                                                                   ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAAGTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   >  2:428652/4‑141 (MQ=11)
                                                                                   tttcaataaAAATGATGATTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTACTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                   <  2:632873/137‑1 (MQ=11)
                                                                                   ctCTAATAGAAATAAAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                     <  2:605654/140‑1 (MQ=255)
                                                                                    tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                  <  1:789501/139‑1 (MQ=11)
                                                                                    ttgaataaAAATGAGGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATACATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTgatcg                                                  <  2:244934/138‑5 (MQ=11)
                                                                                     ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGTAGAGACCTGCGGtt                                                 >  2:326707/2‑141 (MQ=11)
                                                                                     ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                 <  2:751264/140‑1 (MQ=11)
                                                                                     ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                 <  1:613861/140‑1 (MQ=11)
                                                                                     ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGCAGATATGAAGAGACCTGCGGtt                                                 >  2:45472/2‑141 (MQ=11)
                                                                                      taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCGAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                <  2:45145/141‑1 (MQ=11)
                                                                                       aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAAGCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                               <  2:75171/141‑1 (MQ=11)
                                                                                         taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                             <  2:667361/141‑1 (MQ=11)
                                                                                         aataaatggcggggtCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATGTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAACTCCGTAGATCTGAAGAGACCTACGGTTCtt                                              >  2:693870/15‑141 (MQ=1)
                                                                                          aaaaaTAGAAGGTTCTGTTGCAAAGTCTAAAAATATAGCTAACCACTAATTAATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                              >  2:668240/1‑141 (MQ=255)
                                                                                          aaaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                              >  1:777612/1‑141 (MQ=37)
                                                                                          aaaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGAATGATGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATGTGAAGGGACCTGCGGTCCtt                                              >  1:601916/1‑141 (MQ=11)
                                                                                             aaatgatgggtCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGGAGAGACCTGCGGTTCtttttt                                          >  1:534462/11‑140 (MQ=1)
                                                                                              aTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTta                                          <  1:886752/141‑1 (MQ=33)
                                                                                                aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTTCCTGAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                        <  1:218511/141‑1 (MQ=12)
                                                                                                aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAGTTTCGTGGCACGGCGAAAATCCGCAGATATGGAGAGACCTGCgggtgttatgata                                        >  2:497367/1‑130 (MQ=11)
                                                                                                aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                        >  1:79804/1‑141 (MQ=32)
                                                                                                aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGAGCAAATGCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                        >  1:637881/1‑141 (MQ=255)
                                                                                                 gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAAACAGGAGATCGGAGGAGACCTGCGGTTCTTTTtatat                                       >  1:404278/1‑141 (MQ=11)
                                                                                                 gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGGATATCCGTAGGTCTGAGGAGACCTGCGGTTCTTTTtatat                                       >  1:349487/1‑141 (MQ=9)
                                                                                                        ttgctGCAAAGTAAAAAATATGGCTACTCACTAATATATCATGTCAGTGTTGGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATTCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTa                               <  2:373134/137‑1 (MQ=1)
                                                                                                        gtggtGCAAAGGAAAAAAATGTAGCTAACCCCTAATGTATCATGTCAGGGTTCGCTTAACTTGGTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTCCTTTTTATATAGAGCGt                                <  2:48393/137‑1 (MQ=1)
                                                                                                                                ctaataaCTAATATATCTTGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAg        <  2:678215/135‑1 (MQ=1)
                                                                                                                                  aatcACTAAGATATTGTGTCAGTTTTCGTTTAACTTGCTAGCATGATGCTAATTACGTGGCAAGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTGTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa      <  1:28744/138‑1 (MQ=1)
                                                                                                                                    ccccTAATTTATAATGTCTGTTTTCGCCTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCTGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAtt    <  2:113730/138‑1 (MQ=1)
                                                                                                                                      acgaaTTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCt  <  1:544719/138‑1 (MQ=1)
                                                                                                                                            |                                                                                                                                      
CAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCT  >  CP000730/35718‑35992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: