Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	816,291	A→G	4.6%	I11T (ATC→ACC)	USA300HOU_0763 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	816,291	0	A	G	4.6%	231.4 / 2.5	87	I11T (ATC→ACC)	USA300HOU_0763	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (45/37);  new base G (2/2);  total (48/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01

GCTCAGACCAACGCTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCTTTTGTACATA  >  CP000730/816151‑816420                                                                                                                                             |                                                                                                                                  gCTCAGACCAACGCTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGa                                                                                                                                   <  2:737964/141‑1 (MQ=255)    cAGACCAACGCTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTa                                                                                                                                >  2:728486/1‑141 (MQ=255)      gACCAACGCTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATATTGGCGACAGCGTGTTTCAGTATCGATTAAt                                                                                                                              >  2:597729/1‑141 (MQ=255)      gACCAACGCTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAAt                                                                                                                              >  2:839597/1‑141 (MQ=255)           aCGCTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTCTAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGACGACAGCGTGTTTACGTATCGATTAATGAAcc                                                                                                                         >  2:292529/1‑141 (MQ=255)           aCGCTTAATCCCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGaagc                                                                                                                         >  1:691791/1‑139 (MQ=255)            cGCTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTCCAGTATCGATTAATGAACCa                                                                                                                        >  2:168262/1‑141 (MQ=255)              cTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATACTGGCGACAGCGTGTTTCAGTATCGATTCATGAACCATa                                                                                                                      >  1:555814/1‑141 (MQ=255)              cTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATa                                                                                                                      >  2:787246/1‑141 (MQ=255)              cTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATa                                                                                                                      <  2:344027/141‑1 (MQ=255)                  aTGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACt                                                                                                                  <  1:385213/141‑1 (MQ=255)                  aTGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACt                                                                                                                  >  2:933603/1‑141 (MQ=255)                   tGGCGTGCTTTTCAAACTCATTATGGCATTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACtt                                                                                                                 <  2:775386/141‑1 (MQ=255)                   gggCGGGCTTTTCAAGCTCAGTAGGGCACTTATGGCATGGATAGTATTTATGACAACATTTAGATTGAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGGGTTTCAGTATCGATTAATGAACCATAAACtt                                                                                                                 <  2:25744/140‑1 (MQ=255)                         gCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCa                                                                                                           <  2:939407/141‑1 (MQ=255)                          cTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCCGTATCGATTAATGAACCATAAACTTTAGGCAt                                                                                                          >  2:782477/1‑141 (MQ=255)                          cTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCCGTATCAATTAATGAACAATAAACTTTAgccgt                                                                                                          >  2:666510/1‑137 (MQ=255)                           ttttCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAATTTTAGGCATa                                                                                                         <  1:683216/141‑1 (MQ=255)                           ttttCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATa                                                                                                         >  1:870510/1‑141 (MQ=255)                            tttCAAACTCATAATGGCACTGATAAAATAGAATATATTAATTACAACATATAAATTTAATAGCAATAATACCTTCTTCGGCAAAATAATGGCGACAGCGTGTTTCACTACCGATTAATAAACCATAAACTTTAGGCATAg                                                                                                        <  2:526460/141‑1 (MQ=255)                              tCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGAc                                                                                                      >  2:728729/1‑141 (MQ=255)                                aaaCTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGGTTAATGAACCATAAACTTTAGGCATAGACaa                                                                                                    >  1:52453/1‑141 (MQ=255)                                aaaCTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGGTTAATGAACCATAAACTTTAGGCATAGACaa                                                                                                    >  1:799168/1‑141 (MQ=255)                                 aaCTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACCGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACaaa                                                                                                   >  2:409234/1‑141 (MQ=255)                                  aCTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATACTGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATACACTTTAGGCATAGACAAAg                                                                                                  <  1:565119/141‑1 (MQ=255)                                    tCATTATGGCACTTATCGCATGGATAGTATTTATTACAACATTTAAATTTAAGAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCt                                                                                                <  2:718405/141‑1 (MQ=255)                                     cATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGAATAGACAAAACTc                                                                                               >  2:454339/1‑141 (MQ=255)                                     cATTATGGCACTTAGAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTc                                                                                               >  1:504550/1‑141 (MQ=255)                                      attattGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTcc                                                                                              <  1:24510/141‑1 (MQ=255)                                      aTTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTcc                                                                                              >  2:887712/1‑141 (MQ=255)                                           ggCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAAc                                                                                         <  1:63115/141‑1 (MQ=255)                                            ggaCTTATAGCATTGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTTAGTATCGATTAATGAACCATAACCTTTAGGCATAGACAAAGCTCCTTAACt                                                                                        <  2:668561/139‑1 (MQ=255)                                             cACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACtt                                                                                       >  1:283706/1‑141 (MQ=255)                                             cACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGATCCTTAAAtt                                                                                       >  2:172813/1‑141 (MQ=255)                                              aCTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGTTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACCTa                                                                                      <  2:568377/141‑1 (MQ=255)                                                ttATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACg                                                                                    >  2:923195/1‑141 (MQ=255)                                                  ataGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGac                                                                                  >  2:94197/1‑140 (MQ=255)                                                  ataGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGCCCTAGACAAAGCTCATTAACTTACGAt                                                                                  >  2:229992/1‑141 (MQ=255)                                                       aTGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACTATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCtt                                                                             >  1:57221/1‑141 (MQ=255)                                                          gATAATATTTATTACAACATTTAAATTTAATAGCAATAATAGCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCACAAACTTGAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTgg                                                                          <  2:76168/141‑1 (MQ=255)                                                           aTAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGa                                                                         >  2:382056/1‑141 (MQ=255)                                                            tAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGCTTCAGTATCGATTAATGCACCATAAACTTTAGGCAGAGCCAAAGCTCCTGAACTTACGATTCTTTTGGAt                                                                        >  2:415153/1‑141 (MQ=255)                                                             aGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATg                                                                       >  2:293911/1‑141 (MQ=255)                                                               tatttattACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGtt                                                                     <  2:588388/141‑1 (MQ=255)                                                               tatttattACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGtt                                                                     >  2:636740/1‑141 (MQ=255)                                                                  gtattaCAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGAGTAATGAACCATCAAGTTTAGGCATGGGCAACGCTCCTGAACTTACGATTGCTTTGGATGGTCAc                                                                  >  2:68141/2‑141 (MQ=255)                                                                   tattaCAACATGTAAATTTAATAGCAATAATATCTTCTGCGTTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCAcc                                                                 <  2:342829/141‑1 (MQ=255)                                                                       acaacaTTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGCCAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTAAGATTACTTTGGATGTTAAcgcata                                                             >  2:538086/1‑136 (MQ=255)                                                                       acaacaTTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCaata                                                             >  2:882815/1‑141 (MQ=255)                                                                          acaTTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATg                                                          >  1:652361/1‑141 (MQ=255)                                                                           caTTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGGTGTTCACCAATAATGc                                                         <  1:470053/141‑1 (MQ=255)                                                                               taaatttaaTAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATGCCTTTGGATGTTCACCAATAATGCGAAc                                                     <  1:28402/141‑1 (MQ=255)                                                                               taaatttaaTAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATACACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAAc                                                     <  1:28042/141‑1 (MQ=255)                                                                                aaatttaaTAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAGGCTCCTAAACTTACGATTCCATTGGATGTTCACCAAAAATGCGAGCt                                                    >  2:643390/1‑141 (MQ=255)                                                                                aaatttaaTAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCCCAAATAATGCGaaat                                                    >  2:449376/1‑139 (MQ=255)                                                                                aaatttaaTAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACt                                                    >  2:858956/1‑141 (MQ=255)                                                                                aaatttaaTAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACt                                                    >  1:285822/1‑141 (MQ=255)                                                                                       aTAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGTCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGAt                                             <  1:593622/141‑1 (MQ=255)                                                                                       aTAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGAt                                             <  2:365996/141‑1 (MQ=255)                                                                                          gCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTa                                          <  1:231752/141‑1 (MQ=255)                                                                                          gCAATAATATCTTATTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTa                                          <  2:159482/141‑1 (MQ=255)                                                                                            aataatATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAAt                                        >  1:659906/1‑141 (MQ=255)                                                                                            aataatATCTTCTTCGGTAAAATAATGGCGACAGCGTGCATCCGCCACGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAAt                                        >  1:414228/1‑141 (MQ=255)                                                                                                  atCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATg                                  >  1:389133/1‑141 (MQ=255)                                                                                                  atCTGCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGCTTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATg                                  >  1:793444/1‑141 (MQ=255)                                                                                                   acttcttCAGTAAAATAATGGCGAGAGCGTCTTTCATTATCGATTAATGAACCATAAACTTTAGGCATAGACAAATCTCCTTCACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGc                                 <  1:785777/140‑1 (MQ=255)                                                                                                    cttcttCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGcc                                >  1:908553/1‑141 (MQ=255)                                                                                                    cttcttCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATCCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGcc                                <  1:887712/141‑1 (MQ=255)                                                                                                      tcttcGGTAAAATCATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCaa                              <  1:728486/141‑1 (MQ=255)                                                                                                         tcGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAAtt                           >  1:810715/1‑141 (MQ=255)                                                                                                             tAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTc                       <  2:607281/141‑1 (MQ=255)                                                                                                                acatatGGCCATAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCttt                    <  1:117250/137‑1 (MQ=255)                                                                                                                aataatGGCGACAGCGTGTTTCAGTATCGATTAATGAACTATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCttt                    <  2:57221/141‑1 (MQ=255)                                                                                                                aataatGGCGACAGCGTGTTTCAGTATACATTAATGAACCAACAACTTTTAACATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCttt                    <  1:923195/141‑1 (MQ=255)                                                                                                                    atGGCTACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATGTAATTCAATGCCAAATTTTTCTTTGACg                <  1:597729/141‑1 (MQ=255)                                                                                                                    atGGCGACAGCGTGTTTCAGTATCGGTTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACg                <  2:799168/141‑1 (MQ=255)                                                                                                                    atGGCGACAGCGTGTTTCAGTATCGGTTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACg                <  2:52453/141‑1 (MQ=255)                                                                                                                     tGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACgg               <  2:389133/141‑1 (MQ=255)                                                                                                                       gCGACCGCGTGTTTCCGTATCGATTAAGGAGCCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTc             <  2:426042/141‑1 (MQ=255)                                                                                                                        cGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCt            >  1:152345/1‑141 (MQ=255)                                                                                                                          aCAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCttt          <  2:780206/141‑1 (MQ=255)                                                                                                                              ggtgtTTCAGTATCGATTAATGAACCATGAACTTGTGGCCTTGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATATTGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCTTTTGTa      <  2:227077/140‑1 (MQ=255)                                                                                                                               gtgtTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCTTTTGTAc     <  1:382056/141‑1 (MQ=255)                                                                                                                                tgtTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCTTTTGTACa    >  1:511709/1‑141 (MQ=255)                                                                                                                                tgtTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCTTTTGTACa    >  2:72153/1‑141 (MQ=255)                                                                                                                                tgtTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGATCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCTTTTGTACa    >  2:701787/1‑141 (MQ=255)                                                                                                                                tgattCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGCTGTTCACCAATAATGCCAACTTCACGATTTAATTCATTGCAAAATTCTTGTTTAACGGTCTTTAGTACa    >  2:838021/4‑141 (MQ=255)                                                                                                                                  tttCAGTATCGATTAATGAACCATAGACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCTTTTGTACATa  <  1:748181/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                  GCTCAGACCAACGCTTAATGGCGTGCTTTTCAAACTCATTATGGCACTTATAGCATGGATAGTATTTATTACAACATTTAAATTTAATAGCAATAATATCTTCTTCGGTAAAATAATGGCGACAGCGTGTTTCAGTATCGATTAATGAACCATAAACTTTAGGCATAGACAAAGCTCCTTAACTTACGATTCCTTTGGATGTTCACCAATAATGCGAACTTCACGATTTAATTCAATGCCAAATTTTTCTTTGACGGTCTTTTGTACATA  >  CP000730/816151‑816420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑