Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 937,148 T→C 5.0% intergenic (+55/‑1962) USA300HOU_0902 → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730937,1480TC 5.0% 265.4 / 3.6 96intergenic (+55/‑1962)USA300HOU_0902/USA300HOU_0903NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (44/46);  new base C (3/2);  total (47/49)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-01

AGCATTCATGAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAG  >  CP000730/937009‑937287
                                                                                                                                           |                                                                                                                                           
agcaTTCATGAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTc                                                                                                                                            >  1:628038/1‑141 (MQ=255)
  caTTCATGAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCt                                                                                                                                          <  1:561802/141‑1 (MQ=255)
       aTGAAGAAAGTGATTGAGACGCGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGATTTAAATAAAGTTTCCGCTCCACACGGAAAATTAAATTATTCATTTATAAAGTCCTTCAgt                                                                                                                                     <  2:620167/141‑1 (MQ=255)
        tGAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAgtg                                                                                                                                    >  1:617711/1‑141 (MQ=255)
         gAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAgtga                                                                                                                                   <  1:878896/141‑2 (MQ=255)
          aaaaaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTACTTCAGTGTc                                                                                                                                  >  2:289822/1‑141 (MQ=255)
             ataGTGATTGATACACGTGCGGTAGTCAAACGTGGTTGGCTCGGTTTAGCATTCAAAAAATGTAAATTCTAGGCGTTACATAAAGTGTCCGTTAAACCCATATAATTAAATTAGTCATTTATAAAGTCCTTCAGTGTCACt                                                                                                                               <  2:152399/139‑1 (MQ=255)
             aaagtacttgttcaacGTGCGGTATCCAAAAGCGGTGGTATCGGTTTAGCATTCAAAAAAGGAAAATTCTTGGCTTTAAATAAAGTTTCAGCTAAACACATAAAACTAAATTATTCATTTATAATGTCCTTCAGTGTAACt                                                                                                                               <  2:491455/127‑1 (MQ=255)
             aaaGTGTTTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACt                                                                                                                               <  2:846769/141‑1 (MQ=255)
             aaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACt                                                                                                                               <  1:487157/141‑1 (MQ=255)
                  gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAACCACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACGGAAgg                                                                                                                          >  1:61086/1‑141 (MQ=255)
                  gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                          >  2:79527/1‑141 (MQ=255)
                  gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                          >  2:886097/1‑141 (MQ=255)
                    ttgatACACGTGCGGTATTCAAAATCGCGGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATCAAATTAAATTATTCATTTATAAAATCATTCAGTGTCACTGAAGGGc                                                                                                                        >  2:354165/1‑141 (MQ=255)
                     tgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCAATCAAAAAAGGTAGATTGTAGGCTTTAAAGAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCAATGAAGGGCt                                                                                                                       >  1:80716/1‑141 (MQ=255)
                       atACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGGTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAGGACttt                                                                                                                     <  1:635957/141‑1 (MQ=255)
                       atACACGTGCGGTATTCAAAATCGGTCGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAGGACttt                                                                                                                     <  1:299755/141‑1 (MQ=255)
                        tACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCtttt                                                                                                                    >  2:483543/1‑140 (MQ=255)
                         ccacGTGCGGTATTCAAAAGCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCCTTTATAAAGTCCTTCAGGGTCACTGAAGGGCTTTAt                                                                                                                   <  1:458145/140‑1 (MQ=255)
                         acacGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAt                                                                                                                   >  1:694943/1‑141 (MQ=255)
                            cGTGCGGTATGCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATCCATGTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAttgt                                                                                                                <  1:620634/141‑1 (MQ=255)
                              tGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGCGCTTTAttgttg                                                                                                              >  2:303832/1‑141 (MQ=255)
                               gCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCCTTTATAAAGTCCTTCAGTGTCACTGAAGGGCCTTATtgttgt                                                                                                             >  2:211273/1‑141 (MQ=255)
                                gggTATTCAAAACAGGTGGTATCGGTATAGCAGTCAAAAAAGGTAAATTCTAGGCTTGAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                            <  2:1839/140‑1 (MQ=255)
                                cGGTATTTAAAATCGGTGGTATCGGTTTATCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTACATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                            <  2:230616/141‑1 (MQ=255)
                                cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                            <  1:860406/141‑1 (MQ=255)
                                cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                            <  2:628038/141‑1 (MQ=255)
                                 ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                           <  1:937594/141‑1 (MQ=255)
                                 ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                           <  1:565103/141‑1 (MQ=255)
                                  gTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACa                                                                                                          >  2:469378/1‑141 (MQ=255)
                                     ttCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAAtt                                                                                                       <  1:795710/141‑1 (MQ=255)
                                     ttCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAAtt                                                                                                       >  1:23546/1‑141 (MQ=255)
                                        aaaaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGa                                                                                                    <  2:771048/141‑1 (MQ=255)
                                        aaaaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGa                                                                                                    <  1:580023/141‑1 (MQ=255)
                                             cGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGa                                                                                               <  2:323053/141‑1 (MQ=255)
                                              ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGaa                                                                                              >  1:81619/1‑140 (MQ=255)
                                              ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                              >  2:306557/1‑141 (MQ=255)
                                              ggtggtATCGGTTTAGCAGTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                              <  2:495673/141‑1 (MQ=255)
                                               gtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGt                                                                                             <  2:95306/141‑1 (MQ=255)
                                                 ggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACTATTTGAAGTGAGTaa                                                                                           <  1:698243/141‑1 (MQ=255)
                                                   tATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACa                                                                                         <  1:861090/141‑1 (MQ=255)
                                                    aTCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACat                                                                                        >  2:826047/1‑141 (MQ=255)
                                                            aGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGATGTGAGTAACATATAACATc                                                                                >  2:243473/1‑141 (MQ=255)
                                                               aTTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTaa                                                                             >  1:872564/1‑141 (MQ=255)
                                                               aTTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTaa                                                                             <  2:617711/141‑1 (MQ=255)
                                                                ttCAAAAAAGGGAAGTTCTTGGGTTTAAATAAAGGTTCAGCTAAACACATAAAAGTACGTTATGTAGTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAag                                                                            <  1:449290/141‑1 (MQ=255)
                                                                     aaaaGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTTAGTAACATATAAAATCTATGGAGGt                                                                       >  1:165022/1‑141 (MQ=255)
                                                                     aaaaGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGt                                                                       >  1:95943/1‑141 (MQ=255)
                                                                          gTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACAtt                                                                  <  1:811814/141‑1 (MQ=255)
                                                                           tAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTa                                                                 <  1:886097/141‑1 (MQ=255)
                                                                           tAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTAAGTGTCACTGAAGGGCTTTATTGTTGTACAAATTGAAGTGAGTAACATATCAAAACTAAGGAGGTACAATa                                                                 >  1:798474/1‑141 (MQ=255)
                                                                             aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                               <  2:898657/141‑1 (MQ=255)
                                                                                   aGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAtttt                                                         <  2:512580/141‑1 (MQ=255)
                                                                                      cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaa                                                      >  2:166447/1‑141 (MQ=255)
                                                                                      cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaa                                                      >  1:808685/1‑141 (MQ=255)
                                                                                      cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTACTTCAGTGTCACTGAAGTGCTTTATTGTTGTACAATTTAAAGTGAGTAACATATCAAAACTACGGAGGTACATTATGAAattagaa                                                      >  2:828810/1‑137 (MQ=255)
                                                                                          aaataaaGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaa                                                  <  1:273050/141‑1 (MQ=255)
                                                                                          aaataaaGTTTCAGCTAAACACATAAAATTAAATTATTCAGTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaa                                                  >  2:861196/1‑141 (MQ=255)
                                                                                           aataaaGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTAAATTATGAATTttaatttaaa                                                 >  2:409520/1‑141 (MQ=255)
                                                                                           aataaaGTTTCAGATAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaaa                                                 <  1:379659/141‑1 (MQ=255)
                                                                                                 gTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaa                                           >  2:928183/1‑140 (MQ=255)
                                                                                                 gTTTCAGCCAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacac                                           <  1:526498/141‑1 (MQ=255)
                                                                                                  tttCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaca                                          <  1:858739/141‑1 (MQ=255)
                                                                                                  tttCAGCTAAACACAAAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTGGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaca                                          <  2:828447/141‑1 (MQ=255)
                                                                                                   ttCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAATTTAAATAacacac                                         >  1:825205/1‑141 (MQ=255)
                                                                                                      aGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTaa                                      >  2:576191/1‑141 (MQ=255)
                                                                                                        cTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAATGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGc                                    >  1:449211/1‑141 (MQ=255)
                                                                                                                aTAAAATTCAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAAAATTTGAAGTGAGTAAGATATCAAATCTAAGGAGGTACATTATGCATTTTAAATTAAATAAAACACTAAGCAACGaaaa                            >  2:406237/1‑140 (MQ=255)
                                                                                                                 tAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAACTAACACACTAAGCAACGaaata                           >  1:361530/1‑141 (MQ=255)
                                                                                                                 tAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCCACGaaata                           <  2:297337/141‑1 (MQ=255)
                                                                                                                 tAAAATTAAATTATCTATTTATAAAGGCCTTCAATGTCACTGAAGGGCATTATTGTTGTACAATTCGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTTAGCAACGaaata                           <  2:555461/141‑1 (MQ=255)
                                                                                                                  aaaaTTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataa                          <  2:405099/141‑1 (MQ=255)
                                                                                                                   aaattaaattATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataaa                         <  1:469378/141‑1 (MQ=255)
                                                                                                                    aattaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataaat                        >  1:103555/1‑141 (MQ=255)
                                                                                                                     attaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAGGGAGGGACATTATGGATTTTAAATTAAATAACACGCTAAGCGACGAaataaata                       >  2:887692/1‑141 (MQ=255)
                                                                                                                     attaaactaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaata                       <  1:339791/141‑1 (MQ=255)
                                                                                                                      ttatattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATAc                      <  1:774769/141‑1 (MQ=255)
                                                                                                                      ttaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaataa                      >  1:848554/1‑140 (MQ=255)
                                                                                                                       taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGTGGTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGAAACGAagtcaataaa                     >  2:105632/1‑139 (MQ=255)
                                                                                                                       taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCCACGAAATAAATACa                     >  2:939282/1‑141 (MQ=255)
                                                                                                                       taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACa                     <  2:129749/141‑1 (MQ=255)
                                                                                                                       taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGAGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCTAAGCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaataaa                     >  2:395504/1‑139 (MQ=255)
                                                                                                                         aattaTTCATTTATAAAGTGCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTCAAATTAAATAACACACTAAGCAACGAAATAAATACAtt                   >  1:612910/1‑141 (MQ=255)
                                                                                                                           ttattCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGa                 <  2:23546/141‑1 (MQ=255)
                                                                                                                                cATTTATAAAGTCCTTCAGTTGCACTCGAGGGTTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAACCTAAGTAAGAACATTATGACCTTTAAATTAAATAACACACTAAGCAACGACATAAATACATTGattatt            <  2:385999/141‑1 (MQ=255)
                                                                                                                                  acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg          >  1:907029/4‑141 (MQ=255)
                                                                                                                                  acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg          >  1:112027/4‑141 (MQ=255)
                                                                                                                                  acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg          >  1:200657/4‑141 (MQ=255)
                                                                                                                                   ttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGCATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGt         <  1:88702/141‑1 (MQ=255)
                                                                                                                                       aaaGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAc     <  1:352280/141‑1 (MQ=255)
                                                                                                                                        aactCCTGCAGCGTTACTGAAGGGCTTTATTGGTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc    <  2:29573/138‑1 (MQ=255)
                                                                                                                                        aaGTCCTTTCGTGGCCGTGGAGGGCTTTATTGGTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGATGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc    <  2:183054/141‑1 (MQ=255)
                                                                                                                                         aGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCa   >  1:71953/1‑141 (MQ=255)
                                                                                                                                          gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTTGTATACCAg  >  2:586820/1‑141 (MQ=255)
                                                                                                                                          gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg  >  2:586760/1‑141 (MQ=255)
                                                                                                                                          gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg  >  2:720626/1‑141 (MQ=255)
                                                                                                                                          gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg  <  1:289822/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGCATTCATGAAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAG  >  CP000730/937009‑937287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: