Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 937,158 T→A 5.3% intergenic (+65/‑1952) USA300HOU_0902 → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730937,1580TA 5.3% 259.9 / 5.2 95intergenic (+65/‑1952)USA300HOU_0902/USA300HOU_0903NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (45/43);  new base A (3/2);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

AAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAAT  >  CP000730/937019‑937298
                                                                                                                                           |                                                                                                                                            
aaaaaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTACTTCAGTGTc                                                                                                                                             >  2:289822/1‑141 (MQ=255)
   ataGTGATTGATACACGTGCGGTAGTCAAACGTGGTTGGCTCGGTTTAGCATTCAAAAAATGTAAATTCTAGGCGTTACATAAAGTGTCCGTTAAACCCATATAATTAAATTAGTCATTTATAAAGTCCTTCAGTGTCACt                                                                                                                                          <  2:152399/139‑1 (MQ=255)
   aaagtacttgttcaacGTGCGGTATCCAAAAGCGGTGGTATCGGTTTAGCATTCAAAAAAGGAAAATTCTTGGCTTTAAATAAAGTTTCAGCTAAACACATAAAACTAAATTATTCATTTATAATGTCCTTCAGTGTAACt                                                                                                                                          <  2:491455/127‑1 (MQ=255)
   aaaGTGTTTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACt                                                                                                                                          <  2:846769/141‑1 (MQ=255)
   aaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACt                                                                                                                                          <  1:487157/141‑1 (MQ=255)
        gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAACCACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACGGAAgg                                                                                                                                     >  1:61086/1‑141 (MQ=255)
        gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                     >  2:886097/1‑141 (MQ=255)
        gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                     >  2:79527/1‑141 (MQ=255)
          ttgatACACGTGCGGTATTCAAAATCGCGGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATCAAATTAAATTATTCATTTATAAAATCATTCAGTGTCACTGAAGGGc                                                                                                                                   >  2:354165/1‑141 (MQ=255)
           tgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCAATCAAAAAAGGTAGATTGTAGGCTTTAAAGAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCAATGAAGGGCt                                                                                                                                  >  1:80716/1‑141 (MQ=255)
             atACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGGTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAGGACttt                                                                                                                                <  1:635957/141‑1 (MQ=255)
             atACACGTGCGGTATTCAAAATCGGTCGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAGGACttt                                                                                                                                <  1:299755/141‑1 (MQ=255)
              tACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCtttt                                                                                                                               >  2:483543/1‑140 (MQ=255)
               ccacGTGCGGTATTCAAAAGCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCCTTTATAAAGTCCTTCAGGGTCACTGAAGGGCTTTAt                                                                                                                              <  1:458145/140‑1 (MQ=255)
               acacGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAt                                                                                                                              >  1:694943/1‑141 (MQ=255)
                  cGTGCGGTATGCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATCCATGTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAttgt                                                                                                                           <  1:620634/141‑1 (MQ=255)
                    tGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGCGCTTTAttgttg                                                                                                                         >  2:303832/1‑141 (MQ=255)
                     gCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCCTTTATAAAGTCCTTCAGTGTCACTGAAGGGCCTTATtgttgt                                                                                                                        >  2:211273/1‑141 (MQ=255)
                      gggTATTCAAAACAGGTGGTATCGGTATAGCAGTCAAAAAAGGTAAATTCTAGGCTTGAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                       <  2:1839/140‑1 (MQ=255)
                      cGGTATTTAAAATCGGTGGTATCGGTTTATCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTACATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                       <  2:230616/141‑1 (MQ=255)
                      cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                       <  2:628038/141‑1 (MQ=255)
                      cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                       <  1:860406/141‑1 (MQ=255)
                       ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                                      <  1:565103/141‑1 (MQ=255)
                       ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                                      <  1:937594/141‑1 (MQ=255)
                        gTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACa                                                                                                                     >  2:469378/1‑141 (MQ=255)
                           ttCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAAtt                                                                                                                  <  1:795710/141‑1 (MQ=255)
                           ttCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAAtt                                                                                                                  >  1:23546/1‑141 (MQ=255)
                              aaaaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGa                                                                                                               <  1:580023/141‑1 (MQ=255)
                              aaaaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGa                                                                                                               <  2:771048/141‑1 (MQ=255)
                                   cGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGa                                                                                                          <  2:323053/141‑1 (MQ=255)
                                    ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGaa                                                                                                         >  1:81619/1‑140 (MQ=255)
                                    ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                                         >  2:306557/1‑141 (MQ=255)
                                    ggtggtATCGGTTTAGCAGTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                                         <  2:495673/141‑1 (MQ=255)
                                     gtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGt                                                                                                        <  2:95306/141‑1 (MQ=255)
                                       ggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACTATTTGAAGTGAGTaa                                                                                                      <  1:698243/141‑1 (MQ=255)
                                         tATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACa                                                                                                    <  1:861090/141‑1 (MQ=255)
                                          aTCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACat                                                                                                   >  2:826047/1‑141 (MQ=255)
                                                  aGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGATGTGAGTAACATATAACATc                                                                                           >  2:243473/1‑141 (MQ=255)
                                                     aTTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTaa                                                                                        >  1:872564/1‑141 (MQ=255)
                                                     aTTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTaa                                                                                        <  2:617711/141‑1 (MQ=255)
                                                      ttCAAAAAAGGGAAGTTCTTGGGTTTAAATAAAGGTTCAGCTAAACACATAAAAGTACGTTATGTAGTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAag                                                                                       <  1:449290/141‑1 (MQ=255)
                                                           aaaaGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTTAGTAACATATAAAATCTATGGAGGt                                                                                  >  1:165022/1‑141 (MQ=255)
                                                           aaaaGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGt                                                                                  >  1:95943/1‑141 (MQ=255)
                                                                gTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACAtt                                                                             <  1:811814/141‑1 (MQ=255)
                                                                 tAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTa                                                                            <  1:886097/141‑1 (MQ=255)
                                                                 tAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTAAGTGTCACTGAAGGGCTTTATTGTTGTACAAATTGAAGTGAGTAACATATCAAAACTAAGGAGGTACAATa                                                                            >  1:798474/1‑141 (MQ=255)
                                                                   aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                          <  2:898657/141‑1 (MQ=255)
                                                                         aGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAtttt                                                                    <  2:512580/141‑1 (MQ=255)
                                                                            cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaa                                                                 >  2:166447/1‑141 (MQ=255)
                                                                            cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaa                                                                 >  1:808685/1‑141 (MQ=255)
                                                                            cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTACTTCAGTGTCACTGAAGTGCTTTATTGTTGTACAATTTAAAGTGAGTAACATATCAAAACTACGGAGGTACATTATGAAattagaa                                                                 >  2:828810/1‑137 (MQ=255)
                                                                                aaataaaGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaa                                                             <  1:273050/141‑1 (MQ=255)
                                                                                aaataaaGTTTCAGCTAAACACATAAAATTAAATTATTCAGTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaa                                                             >  2:861196/1‑141 (MQ=255)
                                                                                 aataaaGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTAAATTATGAATTttaatttaaa                                                            >  2:409520/1‑141 (MQ=255)
                                                                                 aataaaGTTTCAGATAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaaa                                                            <  1:379659/141‑1 (MQ=255)
                                                                                       gTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaa                                                      >  2:928183/1‑140 (MQ=255)
                                                                                       gTTTCAGCCAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacac                                                      <  1:526498/141‑1 (MQ=255)
                                                                                        tttCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaca                                                     <  1:858739/141‑1 (MQ=255)
                                                                                        tttCAGCTAAACACAAAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTGGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaca                                                     <  2:828447/141‑1 (MQ=255)
                                                                                         ttCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAATTTAAATAacacac                                                    >  1:825205/1‑141 (MQ=255)
                                                                                            aGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTaa                                                 >  2:576191/1‑141 (MQ=255)
                                                                                              cTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAATGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGc                                               >  1:449211/1‑141 (MQ=255)
                                                                                                      aTAAAATTCAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAAAATTTGAAGTGAGTAAGATATCAAATCTAAGGAGGTACATTATGCATTTTAAATTAAATAAAACACTAAGCAACGaaaa                                       >  2:406237/1‑140 (MQ=255)
                                                                                                       tAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAACTAACACACTAAGCAACGaaata                                      >  1:361530/1‑141 (MQ=255)
                                                                                                       tAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCCACGaaata                                      <  2:297337/141‑1 (MQ=255)
                                                                                                       tAAAATTAAATTATCTATTTATAAAGGCCTTCAATGTCACTGAAGGGCATTATTGTTGTACAATTCGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTTAGCAACGaaata                                      <  2:555461/141‑1 (MQ=255)
                                                                                                        aaaaTTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataa                                     <  2:405099/141‑1 (MQ=255)
                                                                                                         aaattaaattATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataaa                                    <  1:469378/141‑1 (MQ=255)
                                                                                                          aattaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataaat                                   >  1:103555/1‑141 (MQ=255)
                                                                                                           attaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAGGGAGGGACATTATGGATTTTAAATTAAATAACACGCTAAGCGACGAaataaata                                  >  2:887692/1‑141 (MQ=255)
                                                                                                           attaaactaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaata                                  <  1:339791/141‑1 (MQ=255)
                                                                                                            ttatattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATAc                                 <  1:774769/141‑1 (MQ=255)
                                                                                                            ttaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaataa                                 >  1:848554/1‑140 (MQ=255)
                                                                                                             taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGTGGTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGAAACGAagtcaataaa                                >  2:105632/1‑139 (MQ=255)
                                                                                                             taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCCACGAAATAAATACa                                >  2:939282/1‑141 (MQ=255)
                                                                                                             taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACa                                <  2:129749/141‑1 (MQ=255)
                                                                                                             taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGAGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCTAAGCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaataaa                                >  2:395504/1‑139 (MQ=255)
                                                                                                               aattaTTCATTTATAAAGTGCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTCAAATTAAATAACACACTAAGCAACGAAATAAATACAtt                              >  1:612910/1‑141 (MQ=255)
                                                                                                                 ttattCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGa                            <  2:23546/141‑1 (MQ=255)
                                                                                                                      cATTTATAAAGTCCTTCAGTTGCACTCGAGGGTTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAACCTAAGTAAGAACATTATGACCTTTAAATTAAATAACACACTAAGCAACGACATAAATACATTGattatt                       <  2:385999/141‑1 (MQ=255)
                                                                                                                        acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                     >  1:907029/4‑141 (MQ=255)
                                                                                                                        acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                     >  1:200657/4‑141 (MQ=255)
                                                                                                                        acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                     >  1:112027/4‑141 (MQ=255)
                                                                                                                         ttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGCATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGt                    <  1:88702/141‑1 (MQ=255)
                                                                                                                             aaaGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAc                <  1:352280/141‑1 (MQ=255)
                                                                                                                              aactCCTGCAGCGTTACTGAAGGGCTTTATTGGTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc               <  2:29573/138‑1 (MQ=255)
                                                                                                                              aaGTCCTTTCGTGGCCGTGGAGGGCTTTATTGGTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGATGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc               <  2:183054/141‑1 (MQ=255)
                                                                                                                               aGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCa              >  1:71953/1‑141 (MQ=255)
                                                                                                                                gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTTGTATACCAg             >  2:586820/1‑141 (MQ=255)
                                                                                                                                gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg             <  1:289822/141‑1 (MQ=255)
                                                                                                                                gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg             >  2:586760/1‑141 (MQ=255)
                                                                                                                                gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg             >  2:720626/1‑141 (MQ=255)
                                                                                                                                      cAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATAGATTGATTATTGGTATACCAGAACAtt       >  1:576622/1‑141 (MQ=255)
                                                                                                                                      cAGTGTCACTGAAGGGCTTCATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATAGGCATACCAGAACAtt       >  2:341114/1‑141 (MQ=255)
                                                                                                                                       agggtCAGTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTAGACCAGAACAttt      >  2:725516/4‑141 (MQ=255)
                                                                                                                                       aGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATAGATTATTGGTCTACCAGAACAttt      >  2:563354/1‑141 (MQ=255)
                                                                                                                                           tCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAAt  >  2:406063/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AAAAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAAT  >  CP000730/937019‑937298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: