Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 937,168 G→A 5.3% intergenic (+75/‑1942) USA300HOU_0902 → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730937,1680GA 5.3% 274.4 / 4.7 95intergenic (+75/‑1942)USA300HOU_0902/USA300HOU_0903NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
Reads supporting (aligned to +/- strand):  ref base G (45/44);  new base A (3/2);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01

TTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAATCAGTTAG  >  CP000730/937029‑937305
                                                                                                                                           |                                                                                                                                         
ttgatACACGTGCGGTATTCAAAATCGCGGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATCAAATTAAATTATTCATTTATAAAATCATTCAGTGTCACTGAAGGGc                                                                                                                                          >  2:354165/1‑141 (MQ=255)
 tgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCAATCAAAAAAGGTAGATTGTAGGCTTTAAAGAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCAATGAAGGGCt                                                                                                                                         >  1:80716/1‑141 (MQ=255)
   atACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGGTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAGGACttt                                                                                                                                       <  1:635957/141‑1 (MQ=255)
   atACACGTGCGGTATTCAAAATCGGTCGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAGGACttt                                                                                                                                       <  1:299755/141‑1 (MQ=255)
    tACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCtttt                                                                                                                                      >  2:483543/1‑140 (MQ=255)
     ccacGTGCGGTATTCAAAAGCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCCTTTATAAAGTCCTTCAGGGTCACTGAAGGGCTTTAt                                                                                                                                     <  1:458145/140‑1 (MQ=255)
     acacGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAt                                                                                                                                     >  1:694943/1‑141 (MQ=255)
        cGTGCGGTATGCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATCCATGTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAttgt                                                                                                                                  <  1:620634/141‑1 (MQ=255)
          tGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGCGCTTTAttgttg                                                                                                                                >  2:303832/1‑141 (MQ=255)
           gCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCCTTTATAAAGTCCTTCAGTGTCACTGAAGGGCCTTATtgttgt                                                                                                                               >  2:211273/1‑141 (MQ=255)
            gggTATTCAAAACAGGTGGTATCGGTATAGCAGTCAAAAAAGGTAAATTCTAGGCTTGAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                              <  2:1839/140‑1 (MQ=255)
            cGGTATTTAAAATCGGTGGTATCGGTTTATCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTACATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                              <  2:230616/141‑1 (MQ=255)
            cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                              <  2:628038/141‑1 (MQ=255)
            cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                              <  1:860406/141‑1 (MQ=255)
             ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                                             <  1:937594/141‑1 (MQ=255)
             ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                                             <  1:565103/141‑1 (MQ=255)
              gTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACa                                                                                                                            >  2:469378/1‑141 (MQ=255)
                 ttCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAAtt                                                                                                                         >  1:23546/1‑141 (MQ=255)
                 ttCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAAtt                                                                                                                         <  1:795710/141‑1 (MQ=255)
                    aaaaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGa                                                                                                                      <  2:771048/141‑1 (MQ=255)
                    aaaaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGa                                                                                                                      <  1:580023/141‑1 (MQ=255)
                         cGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGa                                                                                                                 <  2:323053/141‑1 (MQ=255)
                          ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGaa                                                                                                                >  1:81619/1‑140 (MQ=255)
                          ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                                                >  2:306557/1‑141 (MQ=255)
                          ggtggtATCGGTTTAGCAGTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                                                <  2:495673/141‑1 (MQ=255)
                           gtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGt                                                                                                               <  2:95306/141‑1 (MQ=255)
                             ggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACTATTTGAAGTGAGTaa                                                                                                             <  1:698243/141‑1 (MQ=255)
                               tATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACa                                                                                                           <  1:861090/141‑1 (MQ=255)
                                aTCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACat                                                                                                          >  2:826047/1‑141 (MQ=255)
                                        aGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGATGTGAGTAACATATAACATc                                                                                                  >  2:243473/1‑141 (MQ=255)
                                           aTTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTaa                                                                                               <  2:617711/141‑1 (MQ=255)
                                           aTTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTaa                                                                                               >  1:872564/1‑141 (MQ=255)
                                            ttCAAAAAAGGGAAGTTCTTGGGTTTAAATAAAGGTTCAGCTAAACACATAAAAGTACGTTATGTAGTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAag                                                                                              <  1:449290/141‑1 (MQ=255)
                                                 aaaaGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTTAGTAACATATAAAATCTATGGAGGt                                                                                         >  1:165022/1‑141 (MQ=255)
                                                 aaaaGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGt                                                                                         >  1:95943/1‑141 (MQ=255)
                                                      gTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACAtt                                                                                    <  1:811814/141‑1 (MQ=255)
                                                       tAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTa                                                                                   <  1:886097/141‑1 (MQ=255)
                                                       tAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTAAGTGTCACTGAAGGGCTTTATTGTTGTACAAATTGAAGTGAGTAACATATCAAAACTAAGGAGGTACAATa                                                                                   >  1:798474/1‑141 (MQ=255)
                                                         aaTTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                                 <  2:898657/141‑1 (MQ=255)
                                                               aGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAtttt                                                                           <  2:512580/141‑1 (MQ=255)
                                                                  cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaa                                                                        >  2:166447/1‑141 (MQ=255)
                                                                  cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaa                                                                        >  1:808685/1‑141 (MQ=255)
                                                                  cTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTACTTCAGTGTCACTGAAGTGCTTTATTGTTGTACAATTTAAAGTGAGTAACATATCAAAACTACGGAGGTACATTATGAAattagaa                                                                        >  2:828810/1‑137 (MQ=255)
                                                                      aaataaaGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaa                                                                    <  1:273050/141‑1 (MQ=255)
                                                                      aaataaaGTTTCAGCTAAACACATAAAATTAAATTATTCAGTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaa                                                                    >  2:861196/1‑141 (MQ=255)
                                                                       aataaaGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTAAATTATGAATTttaatttaaa                                                                   >  2:409520/1‑141 (MQ=255)
                                                                       aataaaGTTTCAGATAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaattaaa                                                                   <  1:379659/141‑1 (MQ=255)
                                                                             gTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaa                                                             >  2:928183/1‑140 (MQ=255)
                                                                             gTTTCAGCCAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacac                                                             <  1:526498/141‑1 (MQ=255)
                                                                              tttCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaca                                                            <  1:858739/141‑1 (MQ=255)
                                                                              tttCAGCTAAACACAAAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTGGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaca                                                            <  2:828447/141‑1 (MQ=255)
                                                                               ttCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAATTTAAATAacacac                                                           >  1:825205/1‑141 (MQ=255)
                                                                                  aGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTaa                                                        >  2:576191/1‑141 (MQ=255)
                                                                                    cTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAATGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGc                                                      >  1:449211/1‑141 (MQ=255)
                                                                                            aTAAAATTCAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAAAATTTGAAGTGAGTAAGATATCAAATCTAAGGAGGTACATTATGCATTTTAAATTAAATAAAACACTAAGCAACGaaaa                                              >  2:406237/1‑140 (MQ=255)
                                                                                             tAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAACTAACACACTAAGCAACGaaata                                             >  1:361530/1‑141 (MQ=255)
                                                                                             tAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCCACGaaata                                             <  2:297337/141‑1 (MQ=255)
                                                                                             tAAAATTAAATTATCTATTTATAAAGGCCTTCAATGTCACTGAAGGGCATTATTGTTGTACAATTCGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTTAGCAACGaaata                                             <  2:555461/141‑1 (MQ=255)
                                                                                              aaaaTTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataa                                            <  2:405099/141‑1 (MQ=255)
                                                                                               aaattaaattATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataaa                                           <  1:469378/141‑1 (MQ=255)
                                                                                                aattaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataaat                                          >  1:103555/1‑141 (MQ=255)
                                                                                                 attaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAGGGAGGGACATTATGGATTTTAAATTAAATAACACGCTAAGCGACGAaataaata                                         >  2:887692/1‑141 (MQ=255)
                                                                                                 attaaactaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaata                                         <  1:339791/141‑1 (MQ=255)
                                                                                                  ttatattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATAc                                        <  1:774769/141‑1 (MQ=255)
                                                                                                  ttaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaataa                                        >  1:848554/1‑140 (MQ=255)
                                                                                                   taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGTGGTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGAAACGAagtcaataaa                                       >  2:105632/1‑139 (MQ=255)
                                                                                                   taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCCACGAAATAAATACa                                       >  2:939282/1‑141 (MQ=255)
                                                                                                   taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACa                                       <  2:129749/141‑1 (MQ=255)
                                                                                                   taaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGAGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCTAAGCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAaataaataaa                                       >  2:395504/1‑139 (MQ=255)
                                                                                                     aattaTTCATTTATAAAGTGCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTCAAATTAAATAACACACTAAGCAACGAAATAAATACAtt                                     >  1:612910/1‑141 (MQ=255)
                                                                                                       ttattCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGa                                   <  2:23546/141‑1 (MQ=255)
                                                                                                            cATTTATAAAGTCCTTCAGTTGCACTCGAGGGTTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAACCTAAGTAAGAACATTATGACCTTTAAATTAAATAACACACTAAGCAACGACATAAATACATTGattatt                              <  2:385999/141‑1 (MQ=255)
                                                                                                              acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                            >  1:112027/4‑141 (MQ=255)
                                                                                                              acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                            >  1:200657/4‑141 (MQ=255)
                                                                                                              acaataAAGCCCTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                            >  1:907029/4‑141 (MQ=255)
                                                                                                               ttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGCATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGt                           <  1:88702/141‑1 (MQ=255)
                                                                                                                   aaaGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAc                       <  1:352280/141‑1 (MQ=255)
                                                                                                                    aactCCTGCAGCGTTACTGAAGGGCTTTATTGGTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc                      <  2:29573/138‑1 (MQ=255)
                                                                                                                    aaGTCCTTTCGTGGCCGTGGAGGGCTTTATTGGTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGATGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc                      <  2:183054/141‑1 (MQ=255)
                                                                                                                     aGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCa                     >  1:71953/1‑141 (MQ=255)
                                                                                                                      gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTTGTATACCAg                    >  2:586820/1‑141 (MQ=255)
                                                                                                                      gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg                    >  2:720626/1‑141 (MQ=255)
                                                                                                                      gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg                    <  1:289822/141‑1 (MQ=255)
                                                                                                                      gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg                    >  2:586760/1‑141 (MQ=255)
                                                                                                                            cAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATAGATTGATTATTGGTATACCAGAACAtt              >  1:576622/1‑141 (MQ=255)
                                                                                                                            cAGTGTCACTGAAGGGCTTCATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATAGGCATACCAGAACAtt              >  2:341114/1‑141 (MQ=255)
                                                                                                                             agggtCAGTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTAGACCAGAACAttt             >  2:725516/4‑141 (MQ=255)
                                                                                                                             aGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATAGATTATTGGTCTACCAGAACAttt             >  2:563354/1‑141 (MQ=255)
                                                                                                                                 tCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAAt         >  2:406063/1‑141 (MQ=255)
                                                                                                                                  cACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAATc        >  1:491208/1‑141 (MQ=255)
                                                                                                                                     tGAAGGTCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAATCAGt     <  2:871933/141‑1 (MQ=255)
                                                                                                                                     tGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCGGAACATTTAAATCAGt     >  1:932770/1‑141 (MQ=255)
                                                                                                                                     tGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAATCAGt     <  1:301641/141‑1 (MQ=255)
                                                                                                                                     tGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAATCAGt     <  1:653626/141‑1 (MQ=255)
                                                                                                                                        aGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTAAAATACTTATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAATCAGTTag  <  1:926860/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTAAACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAATCAGTTAG  >  CP000730/937029‑937305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: