Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,035,144 C→T 6.1% intergenic (‑1561/‑6145) USA300HOU_0983 ← / → USA300HOU_0984 competence protein/glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,035,1440CT 6.1% 195.1 / 2.4 70intergenic (‑1561/‑6145)USA300HOU_0983/USA300HOU_0984competence protein/glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (33/30);  new base T (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

AAACATATTCTTCCATTGCTAAGTTTAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGACTTAAAATGACT  >  CP000730/1035012‑1035282
                                                                                                                                    |                                                                                                                                           
acaCATATTGTTCCATTGGTAAGTTTAAAGTTGGATCTGTAATATTATTATTACTACTGAATTTAATTCTTATCTCTCCCTTATAAGTATATATATTCTCTATTTATTTATGCGTTAACTTTGCTCAAAATGGCGATtatt                                                                                                                                     >  2:445914/3‑140 (MQ=255)
aaaCATATTCTTCCATTGCTAAGTTTAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCACATtata                                                                                                                                     >  2:784821/1‑141 (MQ=255)
      atTCTTCCATTGCTAAGTTTAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTca                                                                                                                               <  1:209824/141‑1 (MQ=255)
           tcCATTGCTAAGTTTAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAAt                                                                                                                          >  1:763843/1‑141 (MQ=255)
           tcCATTGCTAAGTTTAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATGTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAAt                                                                                                                          <  2:502122/141‑1 (MQ=255)
                tGCTAAGTTTAAAGTTGTATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTc                                                                                                                    <  2:934058/141‑1 (MQ=255)
                 gCTAAGTTTAAAGTTGGATCTGTAGTATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCt                                                                                                                   <  1:634795/141‑1 (MQ=255)
                    aaGTTTAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTaaa                                                                                                                <  1:670738/141‑1 (MQ=255)
                    aaGTTTAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTaaa                                                                                                                <  2:375157/141‑1 (MQ=255)
                      gTTTAAAGTTGGATCTGTAATATTATTATTACGAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCCTTCTAAata                                                                                                              >  1:675574/1‑141 (MQ=255)
                        ttAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGACAATTATATACTCACAAATCATTCTAAATATc                                                                                                            >  1:715413/1‑141 (MQ=255)
                            aGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCt                                                                                                        <  2:588755/141‑1 (MQ=255)
                                 gATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTCCTTATTTATGCCTTAACTTTGCCCCAAATGCCAATTCTATACTCCCCACCCCTTCTAAACATCGCCTTAAAg                                                                                                   >  1:475330/1‑141 (MQ=255)
                                        aaTATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACAtt                                                                                            <  2:626075/141‑1 (MQ=255)
                                            ttattattaCTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTttat                                                                                        <  2:930419/141‑1 (MQ=255)
                                              attatcaCTAATGAATTTCATTACAATCTCTCCCTGAGATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTttatta                                                                                      <  1:385912/141‑1 (MQ=255)
                                               ttattaCTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATTTCGGCTTAAGGTTACATTTtattat                                                                                     >  1:711294/1‑141 (MQ=255)
                                                tattaCTAATGAATTTCATTACAATCTCTCCCTTATCTCTATATATATTCTCTACTTATTTATGCCTTAATGTTTCTCAAAATTCCAATGATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTattatt                                                                                    <  1:930562/141‑1 (MQ=255)
                                                tattaCTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTattatt                                                                                    <  1:336420/141‑1 (MQ=255)
                                                             tttCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTTTTTGttttt                                                                       >  2:775339/1‑138 (MQ=255)
                                                             tttCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAAt                                                                       <  1:478339/141‑1 (MQ=255)
                                                              ttCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAAGTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATa                                                                      <  1:215479/141‑1 (MQ=255)
                                                               tCATTACAATCTCTCCCTTATATCTATATAGATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAg                                                                     >  2:605374/1‑141 (MQ=255)
                                                                cATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGt                                                                    <  2:783158/141‑1 (MQ=255)
                                                                 aTTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGtt                                                                   >  2:425055/1‑141 (MQ=255)
                                                                 aTTACAATCTCTCCCTTATATATAACTGATTTCTCTACTTATTTCTGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTACATATAGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGtt                                                                   >  2:432887/1‑141 (MQ=255)
                                                                   tACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGTCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTTATTTTTAATAttttt                                                                 >  2:855549/1‑140 (MQ=255)
                                                                   tACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGTCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTa                                                                 >  2:492879/1‑141 (MQ=255)
                                                                   tACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTCGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTa                                                                 >  1:511747/1‑141 (MQ=255)
                                                                    aCAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTAt                                                                >  1:144267/1‑141 (MQ=255)
                                                                       aTCTCTCCCTTATATCTATATATATTCTCTACATATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTg                                                             >  2:913050/1‑141 (MQ=255)
                                                                            tcCCTTATATCTCTATATATTCTCTACTTATTTCTGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAg                                                        <  2:897322/141‑1 (MQ=255)
                                                                            tcCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAg                                                        >  1:462345/1‑141 (MQ=255)
                                                                            tcCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAg                                                        <  1:281657/141‑1 (MQ=255)
                                                                            ccccTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAg                                                        <  1:595207/140‑1 (MQ=255)
                                                                              ccTTATATCTATATATATTCTCTACTTATTTATGGCTTAACTTTGCTCTACATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTg                                                      <  2:757052/141‑1 (MQ=255)
                                                                               cTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGa                                                     >  1:185709/1‑141 (MQ=255)
                                                                               cTTATAGCTATATATATTCTCTACTTATTTATGCCTTGGTTTTGCTTAGAATGCGAGTTACGTACTCACTAATAACTGTAAATTCTGCCAAAAAGGGACAATTTATTATGTTTGATTGTTAAGAGTTTAATTGtgcagcga                                                     <  2:388332/141‑8 (MQ=255)
                                                                                       tagatatatTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGt                                             <  2:745240/138‑1 (MQ=255)
                                                                                          atatctTCTCAACTAATTTCCGCCTTAACTTTGCTCAAACTGCCAACTATATACTCACAAATCATTCTAAATATCGACTTCAGGTTACATTTTATTATTTTTGATTGTTAATATTTTACTTGTTCACTGAATTTTATTAcc                                          >  2:36126/1‑140 (MQ=255)
                                                                                           tatatTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACaa                                         >  1:124496/1‑141 (MQ=255)
                                                                                           tatatTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACaa                                         <  1:333595/141‑1 (MQ=255)
                                                                                                   ttacttactTATGCCTTAACTTGGCTCAAAATGCCAATTACACACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAAttt                                 <  2:50606/140‑1 (MQ=255)
                                                                                                    tACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTa                                <  1:4337/141‑1 (MQ=255)
                                                                                                       ttatttatGCCTTAACTTTGCTCAAAATGTCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGtt                             <  1:492879/141‑1 (MQ=255)
                                                                                                       ttatttatGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGtt                             >  1:341741/1‑141 (MQ=255)
                                                                                                       ttatttatGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGtt                             >  1:821893/1‑141 (MQ=255)
                                                                                                       ttatttatGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGtt                             <  2:558840/141‑1 (MQ=255)
                                                                                                       ttatttatGCCTTAACTTGGCTCAAAATGTCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGtt                             <  1:855549/141‑1 (MQ=255)
                                                                                                        tatttatGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATGGTTTATTTGTTAAGTGAATTTTTGTACAATTTATTTTAGttt                            >  2:681162/1‑140 (MQ=255)
                                                                                                        tatttatGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGGTAAGTGAATTTTAGTACGATATAATTTAGTTa                            >  2:406014/1‑141 (MQ=255)
                                                                                                         atttatGCCTTAACTTTGCTCAAAATGCCAATTATATACTCAAAAATGATTCTCGATATGGGATTGAAGTTTCCTTCTAACAGTATCGATTGTTAATATGGCAGGCGTGAAGTGAATTGTAGGATAATATAATTTAGTTAt                           >  2:81938/1‑141 (MQ=255)
                                                                                                          tttatGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATg                          >  1:191728/1‑141 (MQ=255)
                                                                                                          tttatGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATg                          >  1:788669/1‑141 (MQ=255)
                                                                                                           ttatGCCTTAACTTTTCTCAAAATGCCAATATTATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTTGTACAATATAATTTAGTTATGa                         >  2:244466/1‑141 (MQ=255)
                                                                                                             atGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGAtt                       <  1:603038/141‑1 (MQ=255)
                                                                                                                    aagttttCTCAAAATGGCAATTAAATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTGAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAACTTAGGTATGATTTAaggag                <  1:651867/138‑1 (MQ=255)
                                                                                                                    aacattccccAAAATGCCACTTATCTACTCACAAATCATTCTAAATATCGGCTTCAAGTTACACTGTATTATTTTTGATTGTTAATAGTTTATTTGGTAAGTGAATTTTAGCACAATATAATTTAGTTATGATTTAaggag                <  1:445914/132‑1 (MQ=255)
                                                                                                                    aaCTTTGCTCAAAATGCCAATTATTTACTCACAAATCACTCTAAATATCGGCCTAAAGTTACATTTTATTATTTTTGATTGTTCATAGTGGACTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAaggag                <  1:784821/141‑1 (MQ=255)
                                                                                                                       tttGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGGGGAc             >  2:794913/1‑141 (MQ=255)
                                                                                                                        ttGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTTGTTATGATTTAAGGAGGACt            >  2:497438/1‑141 (MQ=255)
                                                                                                                         tGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGACtt           <  1:913050/141‑1 (MQ=255)
                                                                                                                         tGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGAATATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGACtt           >  1:447325/1‑141 (MQ=255)
                                                                                                                         tGCTCAAAATGCCAATTATATACTACTATTCCATCCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGCCtt           >  1:935404/1‑141 (MQ=255)
                                                                                                                          gCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGACTTa          >  1:145739/1‑141 (MQ=255)
                                                                                                                           cTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGAATTaa         >  1:8456/1‑141 (MQ=255)
                                                                                                                           cTCAAAAGGCCAATTATATACTCACAAAGCATTCTAAATATCGGCTTAAAGTTACAGTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGACTTaa         >  1:597267/1‑141 (MQ=255)
                                                                                                                            tCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGACTTaaa        <  2:511747/141‑1 (MQ=255)
                                                                                                                             cAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGACTTaaaa       >  1:255037/1‑141 (MQ=255)
                                                                                                                                  tGCCAATTATATACGCACAAATCATTCTAAATATCGGCTTAAAGGTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGAGTTAAAATGaag  >  2:715253/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                           
AAACATATTCTTCCATTGCTAAGTTTAAAGTTGGATCTGTAATATTATTATTACTAATGAATTTCATTACAATCTCTCCCTTATATCTATATATATTCTCTACTTATTTATGCCTTAACTTTGCTCAAAATGCCAATTATATACTCACAAATCATTCTAAATATCGGCTTAAAGTTACATTTTATTATTTTTGATTGTTAATAGTTTATTTGTTAAGTGAATTTTAGTACAATATAATTTAGTTATGATTTAAGGAGGACTTAAAATGACT  >  CP000730/1035012‑1035282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: