BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,970	T→C	56.4%	E194E (GAA→GAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,970	0	T	C	56.4%	437.2 / inf	992	E194E (GAA→GAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (230/202); new base C (272/285); total (504/488)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 1000 total aligned reads displayed.

AAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGC  >  CP000730/35837‑36110

agaaagacaagatatcaaagtatcaatacggcaaagaatactttaaaaggtattgaatgtatttacgctctatataaaaagaacggtagatatcttcagatctccggTGCTTTTTATATAAAGCGTaaatacatgtcatac                                                                                                                                       >  1:26558‑M2/108‑134 (MQ=255)

agaaagacaagatatcaaagtatcaatacggcaaagaatactttaaaaggtattgaatgtatttacgctctatataaaaagaaccgtagatctgaagagacatacggTTCTTTTTATATAGAGCGTAAATACATTCAATAc                                                                                                                                       >  2:26558‑M2/108‑141 (MQ=255)

                             tctgtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATa                                                                                                          <  2:593001/138‑1 (MQ=1)

                              cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc                                                                                                         >  1:653355/1‑141 (MQ=11)

                              cAGTGTTCGTTTAACGTGCTAGCATGGTGCTAATCTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTCTTATATAGAGCGTCAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc                                                                                                         <  1:572894/141‑1 (MQ=11)

                               aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                                                        >  1:543837/1‑141 (MQ=11)

                               aGTGTTCGCTTAACTTGCTCGCATGATGCTATTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                        <  2:19136/141‑1 (MQ=9)

                               aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                        <  1:352415/141‑1 (MQ=14)

                                gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                       <  1:20162/141‑1 (MQ=11)

                                gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                       >  1:595317/1‑141 (MQ=255)

                                gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                       >  1:500215/1‑141 (MQ=17)

                                gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                       >  1:837230/1‑141 (MQ=17)

                                  gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCGTGTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTATTTGCCGTATTGATACTTTg                                                                                                     >  2:544628/1‑141 (MQ=11)

                                  gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCG‑AAATCCGTAGGTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCACTACCTTTTAAAGTATTCTCTGCTGTATTGATACTTTGa                                                                                                    <  1:526230/141‑1 (MQ=11)

                                      gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTCTTAAAGTATTCTTTGCTGTATTGATGCTTTGATAc                                                                                                 >  1:509658/1‑141 (MQ=17)

                                       tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                >  1:143980/2‑141 (MQ=17)

                                       tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                <  1:342860/140‑1 (MQ=17)

                                        tgaaCTTGCTAGCATGATGCTAATGTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCt                                                                                               >  2:86831/3‑141 (MQ=11)

                                          accTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                             <  1:168545/139‑1 (MQ=17)

                                          aaCTTGCTAGCATGATGCTACTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCttc                                                                                             >  2:609799/1‑140 (MQ=11)

                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACGTGTTGAATTATTCTTTGCCGTATTGATACTTTGCTATCTTGtc                                                                                           >  1:639060/1‑141 (MQ=11)

                                             ttGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                          <  1:181533/141‑1 (MQ=17)

                                             tcgCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTACATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                                                                          <  2:282774/139‑1 (MQ=11)

                                                cTAGGATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                                                       <  2:581682/141‑1 (MQ=11)

                                                cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGCAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCACTACCTTTTAAAGTATTCTTGGCCGTATTGATGCTTTGATATCTTGtctttt                                                                                       >  2:165836/1‑140 (MQ=11)

                                                cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttt                                                                                       >  2:106163/1‑140 (MQ=17)

                                                  aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                     <  1:41591/141‑1 (MQ=17)

                                                  aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                     <  1:234439/141‑1 (MQ=17)

                                                   gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGGTACTTTGATATCTTGTCTTTCTTa                                                                                    >  2:36430/1‑141 (MQ=11)

                                                   gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                    >  1:283636/1‑141 (MQ=32)

                                                   gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                    >  1:157526/1‑141 (MQ=32)

                                                      tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATGGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTCTTGATACTTTGCTATCTTGTCTTTCTTACtt                                                                                 >  2:209308/1‑141 (MQ=11)

                                                      tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                 <  1:68056/141‑1 (MQ=11)

                                                      tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                 >  2:297884/1‑141 (MQ=11)

                                                      tgatgCTAATTTCGTGGCATGGGGAAAATCCGGAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                 >  1:36354/1‑141 (MQ=11)

                                                      tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                                 >  1:555227/1‑141 (MQ=25)

                                                      tgatgCTAATGTTGGGGCATGGCGAAAATCCGTAGACATTAAAATCCCGGCGTTTCTTTTTATATAGAGCCTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                                                 <  2:331657/141‑1 (MQ=11)

                                                          gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAat                                                                             <  2:313985/141‑1 (MQ=11)

                                                            tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAatat                                                                           >  1:434845/1‑141 (MQ=255)

                                                             aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                                                                          >  1:91087/1‑141 (MQ=11)

                                                             aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatctt                                                                          >  2:134995/1‑138 (MQ=21)

                                                             aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGCTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                          <  2:855930/141‑1 (MQ=25)

                                                                 tCGTGGCATGGGGAAAATCCGTAGATCTGACGAGACCTACGGATCACTTTATATAGAGCGTAACTACACTCTATTCTTTTGAAAGTATTCTTTTCCGTATTCATACTTTGTTGGCTTGTCTTTCTTACTTTAATATGACgg                                                                      >  2:82833/1‑141 (MQ=255)

                                                                 tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATCTGACgg                                                                      >  2:765887/1‑141 (MQ=25)

                                                                  cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                                                     <  2:151787/141‑1 (MQ=255)

                                                                  cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGCTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                                                     <  2:480021/141‑1 (MQ=14)

                                                                  cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAAATAGAGCGTAAATACACTCAATACCTTTTAAAGGATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTACCTTTAAAATGACGGt                                                                     >  1:118373/1‑141 (MQ=11)

                                                                   gTGGCATGGCGAAAATCCGTAGATCTGACGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTTTTACTTTAATATGACGGTg                                                                    >  2:535501/1‑141 (MQ=255)

                                                                    tGGCATGGCGAAAATCCGTAGATCTGAAAAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                   >  2:668093/1‑141 (MQ=255)

                                                                        aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTAATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCtt                                                               >  2:459681/1‑141 (MQ=255)

                                                                         tggggAAAATCCGAAGATCGGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                              >  2:912245/5‑141 (MQ=14)

                                                                         tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                              <  1:868835/141‑1 (MQ=255)

                                                                          gggcaaaaTTCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                             <  1:132610/137‑1 (MQ=11)

                                                                          ggCGAAAATCCGTAGTTCTGAAGCGACCGGCGGTTCTTTTGATATAGAGCGTAAATACACTCAATACCCTTTAATGTATTCTTTGCTGTATCGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                             <  1:21657/141‑1 (MQ=11)

                                                                          ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATCCACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                             >  1:89146/1‑141 (MQ=255)

                                                                          ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                             >  2:841233/1‑141 (MQ=38)

                                                                           gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                            <  1:481631/141‑1 (MQ=255)

                                                                             gAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGTCGGTGATCTTGCTCa                                                          <  1:700924/141‑1 (MQ=255)

                                                                               aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                        <  1:30590/141‑1 (MQ=255)

                                                                                 aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                      <  1:100967/141‑1 (MQ=255)

                                                                                   CCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                    >  2:745661‑M1/1‑141 (MQ=255)

                                                                                     GTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACAGTGATCTTGATCAATGAGGtt                                                  <  1:77662‑M1/141‑1 (MQ=255)

                                                                                            tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                           <  1:626281/141‑1 (MQ=255)

                                                                                            tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                           <  2:63144/141‑1 (MQ=255)

                                                                                            gtaaGAGACCTGCGGTTCTTTTTAGATTGAGGGTAAATACATTCAATACCCTTTAAAGTATTCTTTGCTGTATTGACACTTTTATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                           <  2:806520/139‑1 (MQ=11)

                                                                                             gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat                                          <  1:247604/141‑1 (MQ=255)

                                                                                              aaGAGACCTGCGGTTTTTTTTATATAGAGCGTAGATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                         <  2:883216/141‑1 (MQ=255)

                                                                                              aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTATAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                         >  1:668378/1‑141 (MQ=255)

                                                                                               agagACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat                                        >  2:716951/1‑141 (MQ=255)

                                                                                                 agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTGTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAttgcgagagtt                                      >  2:380708/1‑132 (MQ=11)

                                                                                                  gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtttt                                     >  2:780839/1‑140 (MQ=255)

                                                                                                     cTGCGGTTCTTTTGATATAGAGCGTAAATACACTCAATACCTTTTAAAGTGTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                                  <  2:644420/141‑1 (MQ=255)

                                                                                                       gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                >  1:851238/1‑141 (MQ=255)

                                                                                                         ggTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAc                              <  1:453984‑M2/139‑1 (MQ=255)

                                                                                                          gTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGATGTACa                             >  1:394830‑M2/2‑141 (MQ=255)

                                                                                                            tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCATTGTGGTTATTCAGATATTTCGATGTACCAt                           >  2:928744/1‑141 (MQ=255)

                                                                                                             cTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                          <  2:425086/141‑1 (MQ=255)

                                                                                                             cTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGGTGTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                          >  2:903288/1‑141 (MQ=255)

                                                                                                              tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGGTGTACAATgg                         >  1:825059/1‑140 (MQ=255)

                                                                                                               ttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAc                        >  1:379195/1‑141 (MQ=255)

                                                                                                               ttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggc                        >  2:448796/1‑139 (MQ=255)

                                                                                                                tttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAAATGACGGTGATCTTGCACCCTGAAGTTATTAAGATATCTAGACGTACAATGGCa                       <  2:225326/141‑1 (MQ=11)

                                                                                                                    tataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCa                   <  1:802137/141‑1 (MQ=255)

                                                                                                                     ataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                  <  1:763770/141‑1 (MQ=255)

                                                                                                                       agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgt                >  2:495877/1‑141 (MQ=255)

                                                                                                                        gagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGTCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt               >  2:512011/1‑141 (MQ=255)

                                                                                                                        gagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgggt               >  1:606382/1‑139 (MQ=255)

                                                                                                                            gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag           >  1:79505/1‑141 (MQ=255)

                                                                                                                             tAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagt          >  1:293615/1‑141 (MQ=255)

                                                                                                                              aaaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaggtt         >  1:741925/1‑141 (MQ=255)

                                                                                                                              aaaTACACTCAATACCTTTTAAAGTAAACATTTCAGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt         >  1:692507/1‑141 (MQ=14)

                                                                                                                               aaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCTATGTACAATGACAGTCAGgtttaagttt        >  1:469488/1‑141 (MQ=255)

                                                                                                                               aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCTCAGTACAATGGCAGTCAGgtttaagttt        <  1:935697/141‑1 (MQ=14)

                                                                                                                                aTACATTCAATACCTTTTAACGTATTCTTTGCTGTATTGATACTTAGCGACATACAAGTTCTCACTATTAGTTGACGTTTATCTTGCTCAATTAGCGTATTCAGATAGTTCGATTTACAATGACAGTCAGgtttaagttta       <  2:690151/141‑1 (MQ=9)

                                                                                                                                aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta       >  2:408675/1‑141 (MQ=255)

                                                                                                                                 tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa      >  2:250314/1‑141 (MQ=255)

                                                                                                                                 tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa      >  1:88024/1‑141 (MQ=255)

                                                                                                                                 tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGAGATTTCGATGTACAATGACAGTCAGgtttaagtttga      >  2:564444/1‑139 (MQ=255)

                                                                                                                                 tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa      >  1:715307/1‑141 (MQ=255)

                                                                                                                                  aCATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaa     >  1:323927/1‑141 (MQ=255)

AAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGC  >  CP000730/35837‑36110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`