Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,305,823 C→T 12.2% intergenic (‑126/‑51) USA300HOU_2163 ← / → USA300HOU_2164 hypothetical membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,305,8230CT12.2% 279.7 / 23.5 111intergenic (‑126/‑51)USA300HOU_2163/USA300HOU_2164hypothetical membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (49/48);  new base T (12/2);  total (61/50)
Fisher's exact test for biased strand distribution p-value = 1.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01

Only 100 of 116 total aligned reads displayed.

TGTTTTACCTGTCATATTTGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAATTAAAA  >  CP000730/2305683‑2305961
                                                                                                                                            |                                                                                                                                          
tgtTTTACCTGTCATCTTTGTTCTCCCTTAATACGCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTGAAATTCATTAGTACGACACAATTTAAAAAAGTATCACCTAACTCTTGAAAACGATTAc                                                                                                                                            <  1:573557/141‑1 (MQ=255)
tgtTTTACCTGTCATAGTTGTTCTCCTTTAATACTAACACTTTCCCACATCCCTAACAAAAACACGAACATCATTTCGCGTTAAAATTGATTATTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTAc                                                                                                                                            <  2:856800/141‑1 (MQ=255)
tgtTTGACCTGTCAGCTTTGTTCTCATTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATGTAAAAAAGTATCACATAACTCTTGAAAACGATTAc                                                                                                                                            <  2:443076/141‑1 (MQ=255)
   tttACCTGTCATATTTGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACaaa                                                                                                                                         >  1:803314/1‑141 (MQ=255)
   tttACCTGTCATATTTGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACaaa                                                                                                                                         <  2:515579/141‑1 (MQ=255)
    ttACCTGTCATATTTGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTGATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACaaaa                                                                                                                                        >  1:695653/1‑141 (MQ=255)
     tACCTGTCATATTTGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAAt                                                                                                                                       >  1:758736/1‑141 (MQ=255)
       ccTGTCATATTTGTTCTCCTTCAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCg                                                                                                                                     <  1:318207/141‑1 (MQ=255)
        cTGTCATATTTGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGt                                                                                                                                    >  2:161640/1‑141 (MQ=255)
              tttttGTTCTACTTTAAAAATCACATGATACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTAAAAAAAAAAAAAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTatga                                                                                                                              <  2:918180/139‑1 (MQ=255)
                tttGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTatgatg                                                                                                                            >  1:540175/1‑141 (MQ=255)
                tttGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTATatgatt                                                                                                                            >  2:139803/1‑140 (MQ=255)
                 ttGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGt                                                                                                                           <  2:189087/141‑1 (MQ=255)
                    ttCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTAtt                                                                                                                        >  1:536246/1‑141 (MQ=255)
                      ctcCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGCCACAATTTAAAAAAGTAACACATAACTCTTGAAAACGATTATAAAAACGTTTATAATGTAtttc                                                                                                                      >  2:938508/1‑140 (MQ=255)
                      ctcCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTATAAAATCGTTTATGATGTATTTa                                                                                                                      >  1:114341/1‑141 (MQ=255)
                      ctcCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATAGTTTATGATGTATTTa                                                                                                                      >  2:861249/1‑141 (MQ=255)
                         cTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACCTAACTCTTGAAAACGGATACAAAATCGTTTATGATGTATCTACaa                                                                                                                   >  2:56503/1‑141 (MQ=255)
                         cTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACaa                                                                                                                   >  2:660135/1‑141 (MQ=255)
                         cTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACaa                                                                                                                   >  1:403685/1‑141 (MQ=255)
                             aaTACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGGTTATGATGTATTTACaaaaaa                                                                                                               >  2:4274/1‑139 (MQ=255)
                              aTACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAatat                                                                                                              <  2:624156/141‑1 (MQ=255)
                               tgcTCACATTTTGCCACATCCTTAAAAAAAACTCAACTTTATTTTCGGGGTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAATTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATAtt                                                                                                             <  2:427079/139‑1 (MQ=255)
                                   cacaTTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTATAAAATCGTTTATGATGTATTTACAAAATATTTaaa                                                                                                         >  1:352959/1‑141 (MQ=255)
                                       ttttACCACATCCCTAACAAAAACACGAACATCTTTTCGGGTTAAAATTCATGAGGATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTCAAGGAt                                                                                                     >  1:222803/1‑141 (MQ=255)
                                           aCCACATCCCTAACAAAAACGCGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTATAAAATCGTTTATGATGCATTTACAAAATATTTAAAGGATgtgt                                                                                                 >  1:573206/1‑141 (MQ=255)
                                           aCCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATgtgt                                                                                                 >  1:155619/1‑141 (MQ=255)
                                           aCCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATgtgt                                                                                                 >  1:739708/1‑141 (MQ=255)
                                           aCCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATgtgt                                                                                                 >  2:243677/1‑141 (MQ=255)
                                           aCCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATgtgt                                                                                                 >  2:291127/1‑141 (MQ=255)
                                            ccACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGtt                                                                                                >  1:205228/1‑141 (MQ=255)
                                                ctCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGaa                                                                                            <  1:300829/140‑1 (MQ=255)
                                                aTCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACTAAATATTTAAAGGATGTGTTTGaa                                                                                            <  1:603073/141‑1 (MQ=255)
                                                   ccTACCAAAAACACGAACATATTTTCGGGTTAAACTTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGaataa                                                                                         <  2:946086/141‑1 (MQ=255)
                                                     tAACAAAAGCACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATg                                                                                       <  1:477142/141‑1 (MQ=255)
                                                     tAACAAAAACACGAACATATTTTCGGGTTAAACTTCATTAGTATGACCCAATTTAAAAAAGTATCGCATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACTGAATATTTAAAGGATTTGTTTGAATATTg                                                                                       >  2:754458/1‑141 (MQ=255)
                                                      aaCAAAAACAGAAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATgg                                                                                      <  2:403859/141‑1 (MQ=255)
                                                      aaCAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATgg                                                                                      <  1:42692/141‑1 (MQ=255)
                                                      aaCAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATAGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATgg                                                                                      >  2:832997/1‑141 (MQ=255)
                                                             acacGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGAc                                                                               >  2:241928/1‑141 (MQ=255)
                                                              cacGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACa                                                                              >  2:599900/1‑141 (MQ=255)
                                                                cGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGCTGAGTTTGAATAATGGCAATGACAGt                                                                            >  1:401478/1‑141 (MQ=255)
                                                                cGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGt                                                                            >  2:2323/1‑141 (MQ=255)
                                                                  aaCATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTaa                                                                          >  1:198244/1‑141 (MQ=255)
                                                                    cATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTaaaa                                                                        >  2:395927/1‑141 (MQ=255)
                                                                    aatatTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTaaaa                                                                        <  2:393730/140‑1 (MQ=255)
                                                                          ttCGGGTTAAAATTCAGTACTATGACACAATTTAAAAATGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATCTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAAGGACAGTAAAAAAGGat                                                                  >  1:527468/1‑141 (MQ=255)
                                                                              ggctAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGataata                                                              <  2:939355/138‑1 (MQ=255)
                                                                              ggTTAAAATTCATTATTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATGACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGataata                                                              <  2:334267/141‑1 (MQ=255)
                                                                              ggTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGataata                                                              <  2:540175/141‑1 (MQ=255)
                                                                              ggTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCCTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGataata                                                              <  1:881426/141‑1 (MQ=255)
                                                                                ttAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATaataat                                                            <  1:662924/141‑1 (MQ=255)
                                                                                 aaaaaTTAAGTAGTATGACACAATTTAAAAAAGTATCACAGAACCCGTGAAAACGATTATAGAAGCGTTTCCGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGAAAAAAAAAAAAAAAAGGATAATAATg                                                           <  2:569059/140‑1 (MQ=255)
                                                                                    aaTTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAg                                                        <  2:758736/141‑1 (MQ=255)
                                                                                      ttCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTg                                                      >  2:634554/1‑141 (MQ=255)
                                                                                      ttCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTg                                                      >  1:422727/1‑141 (MQ=255)
                                                                                       tCATTAGTATGACACACTATAAAAAAGTATCACATAACTCTTGAAAACGATTATAAAATCGATTATGATGTATTTACAAAAAATTTAAAGGAAGTGTTTGAATAATGGCAATGACAGTCAAAAAAGAAAAGAATGAAGAGc                                                     >  2:850213/1‑141 (MQ=255)
                                                                                       tCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTATAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGc                                                     >  2:552882/1‑141 (MQ=255)
                                                                                         aTTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGt                                                   >  1:402959/1‑141 (MQ=255)
                                                                                         aTTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGt                                                   <  2:739708/141‑1 (MQ=255)
                                                                                         aTTAGTATGACAAAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGt                                                   >  1:666518/1‑141 (MQ=255)
                                                                                           tAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTATAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGTAATGACAGTAAAAAAGGATAATAATGAAGTGCGTAt                                                 >  1:522795/1‑141 (MQ=255)
                                                                                              tATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCa                                              >  1:671503/1‑141 (MQ=255)
                                                                                                 gACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATg                                           <  2:29323/141‑1 (MQ=255)
                                                                                                  aaacatactAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATgg                                          <  2:423389/133‑1 (MQ=255)
                                                                                                    acaATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGATTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGgag                                        >  1:323228/1‑141 (MQ=255)
                                                                                                    acaATTTAAAAAAGTATCACATAACTAATGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGgag                                        <  2:118468/141‑1 (MQ=255)
                                                                                                    aacatatAAAAAAGTATCACATAACTCTTGGATACGGTTACAGAATCGTTTGTGGTGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGgag                                        <  2:371730/135‑1 (MQ=255)
                                                                                                      aaTTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATACAATGGaaag                                      >  2:312125/1‑141 (MQ=255)
                                                                                                       aTTTAAAAAAGTATCACATAACTCTTGAAAACGATTATAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGt                                     >  1:102873/1‑141 (MQ=255)
                                                                                                       aTTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGt                                     >  1:411837/1‑141 (MQ=255)
                                                                                                        tttAAAAAAGTTTTACATAACTCTTGAAAACTATTACAAAATCGTTTAGTATGTATTTATAAAATATTTAAAGGATGTGTTTGAATATTTGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGtt                                    <  2:261078/141‑1 (MQ=255)
                                                                                                        tttAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGtt                                    <  2:845827/141‑1 (MQ=255)
                                                                                                          tAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGc                                  >  1:533264/1‑141 (MQ=255)
                                                                                                              aaaGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGat                              <  2:666518/141‑1 (MQ=255)
                                                                                                               aactaccacaTAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGata                             <  1:468465/135‑1 (MQ=255)
                                                                                                                aatATCACATAACTCTTGAAAGCGATTACAAAATCGTTTATGATGTATGTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAATGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGatat                            <  2:23241/139‑1 (MQ=255)
                                                                                                                 gTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATc                           >  2:43278/1‑141 (MQ=255)
                                                                                                                  tttCACATAATCCTTGAAAACGATTACAAAATGGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGTTAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCa                          <  2:842962/139‑1 (MQ=255)
                                                                                                                  tATCCCATAACTCTTGAAAACGATTACAAAAGCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCa                          <  1:74566/141‑1 (MQ=255)
                                                                                                                  tATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCa                          <  1:744250/141‑1 (MQ=255)
                                                                                                                     cacaTAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCaaaa                       <  1:241928/141‑1 (MQ=255)
                                                                                                                         tAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTcc                   <  2:587333/141‑1 (MQ=255)
                                                                                                                           aCTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTa                 >  1:719090/1‑141 (MQ=255)
                                                                                                                           aCTCTTGAAAACGATTACAAAAGCGTTTATGATGTATGTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTa                 <  2:399429/141‑1 (MQ=255)
                                                                                                                            ctctTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAACAGGATAATCATGAAGTGCGTATTCAAAGGAGCGTTGCTGATTTCAATATTCCtcc                <  2:693389/141‑3 (MQ=255)
                                                                                                                            ctctTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTAc                <  1:88320/141‑1 (MQ=255)
                                                                                                                             tctTGAAAACGATTACAAAAGCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGAAAAAAAAAAAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACa               <  2:803314/141‑1 (MQ=255)
                                                                                                                                tGAAAACGATTATAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTCCacgg            >  2:873968/1‑138 (MQ=255)
                                                                                                                                  aaaaCGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGa          <  1:360765/141‑1 (MQ=255)
                                                                                                                                  aaaaCGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGa          <  1:718510/141‑1 (MQ=255)
                                                                                                                                      cGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAAtt      >  2:556580/1‑141 (MQ=255)
                                                                                                                                      cGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAAtt      >  1:29233/1‑141 (MQ=255)
                                                                                                                                      cGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGGTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAAtt      >  1:413045/1‑141 (MQ=255)
                                                                                                                                        aTTATAAAATCGTTTATGATGTATTTACGAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAATTaa    >  1:597864/1‑141 (MQ=255)
                                                                                                                                        aTTATAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTACTACACGTGAAATTaa    >  1:816177/1‑141 (MQ=255)
                                                                                                                                        aTTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAATTaa    >  2:715512/1‑141 (MQ=255)
                                                                                                                                        aTTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAATTaa    <  2:671503/141‑1 (MQ=255)
                                                                                                                                        aTTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTAAAATTaa    >  2:766173/1‑141 (MQ=255)
                                                                                                                                          tACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAATTaaaa  <  1:489300/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TGTTTTACCTGTCATATTTGTTCTCCTTTAATACTCACATTTTACCACATCCCTAACAAAAACACGAACATATTTTCGGGTTAAAATTCATTAGTATGACACAATTTAAAAAAGTATCACATAACTCTTGAAAACGATTACAAAATCGTTTATGATGTATTTACAAAATATTTAAAGGATGTGTTTGAATAATGGCAATGACAGTAAAAAAGGATAATAATGAAGTGCGTATTCAATGGAGAGTTGCTGATATCAAAATTCCTACAAGTGAAATTAAAA  >  CP000730/2305683‑2305961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: