Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 19,264 G→A 27.8% G307D (GGC→GAC)  USA300HOU_0014 → DHH subfamily phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073019,2640GA27.8% 196.2 / 76.4 121G307D (GGC→GAC) USA300HOU_0014DHH subfamily phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (47/40);  new base A (21/13);  total (68/53)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TATCGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCA  >  CP000730/19130‑19403
                                                                                                                                      |                                                                                                                                           
tATCGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTaa                                                                                                                                       <  1:812136/141‑1 (MQ=255)
  tCGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGCaaca                                                                                                                                     >  2:293808/1‑139 (MQ=255)
   cGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGAc                                                                                                                                    >  2:1144246/1‑141 (MQ=255)
   cGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCAAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGATTCTATGGCGGTAAGAc                                                                                                                                    >  2:803004/1‑141 (MQ=255)
   cGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATCACCTTCAACAATTCTAGACTTCGAATGAGGACTCGGTGGCGACAAAGTTGCCATTAAATGTATTAATGGGGATGTGCGTTTCTATGGCGGTAAGAc                                                                                                                                    >  1:280622/1‑141 (MQ=255)
    ggTGTTGGTGTAGGTCCTGAAGATCGCCTCAACATAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTACTATGGCGGTAAGACt                                                                                                                                   >  2:846648/1‑141 (MQ=255)
      tgtTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGAGCGTTTCTATGGCGGTAAGACTGa                                                                                                                                 >  2:748299/1‑141 (MQ=255)
      ggtTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTAAAGTAACACAAGCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTTAAAGTATGACTGGTAAAGTGAGTTTCTATGGCGGAAAGAGTGa                                                                                                                                 >  2:227602/2‑141 (MQ=255)
         tGGTGAAGGTACTGAAAATTTATTCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGAccc                                                                                                                              <  2:409102/141‑1 (MQ=255)
         tGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAGTCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGGTTCTATGGCGGTAAGGCTGGccc                                                                                                                              >  2:1046380/1‑141 (MQ=255)
           gTGAAGGTTCTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTATGCTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  1:244318/141‑1 (MQ=255)
           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  2:316343/141‑1 (MQ=255)
           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  1:1058368/141‑1 (MQ=255)
           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            >  1:444242/1‑141 (MQ=255)
           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            <  1:1002062/141‑1 (MQ=255)
           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            >  2:667443/1‑141 (MQ=255)
           gTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGa                                                                                                                            >  2:924205/1‑141 (MQ=255)
            tGAAGGTACTGCAAATTTAATCGCCTTAGGTGAATTATCACAATCAGGCCTAGACCTAGCATTAGGCCGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAACGGGCGTTCCTATGGCGGCAAGaagaacccgcg                                                                                                                           >  2:588781/1‑131 (MQ=255)
                       aaaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTa                                                                                                                <  1:166335/141‑1 (MQ=255)
                       aaaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTa                                                                                                                <  1:305548/141‑1 (MQ=255)
                       aaaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTa                                                                                                                <  2:860988/141‑1 (MQ=255)
                       aaaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTa                                                                                                                >  1:313350/1‑141 (MQ=255)
                        aaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTAc                                                                                                               <  1:172787/141‑1 (MQ=255)
                            ttAATCGTGTTAGGTGAATTATGACAATCAGGCCTAGACTTAGCAGTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTGAGACTGACCCGATGGAGAAACGTACTCgt                                                                                                           <  2:582141/141‑1 (MQ=255)
                              aaTCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAAGGTGCGTTTCTATGACGGTAAGCCTGACCCGAGGGAGAAAGGTACTctggg                                                                                                         >  2:1114597/1‑137 (MQ=255)
                               aTCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTa                                                                                                        <  1:717083/141‑1 (MQ=255)
                               aTCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTa                                                                                                        >  2:422723/1‑141 (MQ=255)
                                 cGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTTTAag                                                                                                      >  2:830993/1‑141 (MQ=255)
                                 cGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAag                                                                                                      >  2:334515/1‑141 (MQ=255)
                                     ttAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGAAGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAGACGTACTCGTGTAAGAGCa                                                                                                  >  2:233040/1‑141 (MQ=255)
                                       aGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAGTGGGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAAAGTCCTCGTGTAAGGACACg                                                                                                >  2:457510/1‑141 (MQ=255)
                                       aGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACg                                                                                                <  2:542731/141‑1 (MQ=255)
                                       aGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACg                                                                                                >  1:21115/1‑141 (MQ=255)
                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACgtggg                                                                                            >  1:1080122/1‑139 (MQ=255)
                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGtgtg                                                                                            <  1:981943/141‑1 (MQ=255)
                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGtgtg                                                                                            <  1:982014/141‑1 (MQ=255)
                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTGAAAGCACGtttg                                                                                            >  2:418531/1‑141 (MQ=255)
                                           gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGtgtg                                                                                            >  2:433614/1‑141 (MQ=255)
                                                aTCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGAtctc                                                                                       >  2:900485/1‑141 (MQ=255)
                                                  cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAACAGTCTTAGTGGTAATGTGCGTTTCTCTGACGGTAAGACTGGCCCGACGGAGAAACGTAGTCGTGCGTGAGCAGGTGTGaggtcgc                                                                                     >  1:13455/1‑135 (MQ=255)
                                                  cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTcac                                                                                     >  1:1035126/1‑141 (MQ=255)
                                                  cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTcac                                                                                     >  2:1173267/1‑141 (MQ=255)
                                                  cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTcac                                                                                     >  1:478527/1‑141 (MQ=255)
                                                   acaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGGGTAAGAGCACGTGTGATCTcaca                                                                                    >  2:673864/1‑141 (MQ=255)
                                                    caATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                   <  2:280622/141‑1 (MQ=255)
                                                    caATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                   <  1:542601/141‑1 (MQ=255)
                                                       tCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCGATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                   >  2:652851/1‑138 (MQ=255)
                                                       tCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                   <  1:652851/138‑1 (MQ=255)
                                                         aGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGtt                                                                              >  1:782241/1‑141 (MQ=255)
                                                           gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                            >  1:1196721/1‑141 (MQ=255)
                                                           gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                            >  1:708139/1‑141 (MQ=255)
                                                           gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                            <  1:226907/141‑1 (MQ=255)
                                                                gACTGAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGat                                                                       <  1:526682/141‑1 (MQ=255)
                                                                    tAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATcc                                                                   >  2:1184972/1‑141 (MQ=255)
                                                                     aGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCt                                                                  <  1:141301/141‑1 (MQ=255)
                                                                      gCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGAGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCCCGTGTGATCTCACATGCGTCAAAAGATATCCtt                                                                 >  2:271367/1‑141 (MQ=255)
                                                                       cATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTg                                                                <  1:900485/141‑1 (MQ=255)
                                                                         ttAGGACGCGTGGGCGACCAAGTTGCAATTAAAAGTATTAAAGGTAATGTGCGTTTCTATGGCCGTAAGACTGACCCGATGTAGAACCGTCCTCGTGTAAGAGCACGTGTGATCTCAAATGCTTTAAAAGATCTGCTTgta                                                              <  2:635146/141‑3 (MQ=255)
                                                                         ttAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCa                                                              >  1:952935/1‑141 (MQ=255)
                                                                         ttAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCa                                                              >  1:185129/1‑141 (MQ=255)
                                                                         ttAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTACTAATGCTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCTTGTAAGAGCACGTGTGATCTAACATGCGTTAAATGATATCCATGCa                                                              <  1:843662/141‑1 (MQ=255)
                                                                            ggACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCcgag                                                           >  1:266266/1‑141 (MQ=255)
                                                                            ggACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTGTCAAAGGGGGTAAGAGTGTCCCGATGGTGACACGTATTCTTGTGAGAGCACGTGTGATCTAACATGCGTTATAAGATATCCTTGCagag                                                           >  2:299272/1‑141 (MQ=255)
                                                                              aCGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGGTATCCTTGCAGAggg                                                         >  2:601395/1‑141 (MQ=255)
                                                                               cgcgGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGt                                                        <  1:667443/141‑1 (MQ=255)
                                                                               cgcgGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAAGGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGt                                                        <  1:160693/141‑1 (MQ=255)
                                                                                 cgGTGGCGACCAACTTGCAATTAACAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGa                                                      <  1:511808/141‑1 (MQ=255)
                                                                                  ggTGGCGGCCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGAc                                                     <  1:373824/141‑1 (MQ=255)
                                                                                    tGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACaa                                                   <  1:924205/141‑1 (MQ=255)
                                                                                     ggCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACaaa                                                  >  1:322839/1‑141 (MQ=255)
                                                                                      gCGACCAAGTTGCAATTAAAAGTATTCATGGTAATGGGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGGTCTCGCATGCGTTAAAAGATATCCTTGCAGAGGGGGGCAAAg                                                 >  2:564490/1‑141 (MQ=255)
                                                                                      gCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGGTCTCACATGCGTTCAAAGATATCCTTGCAGAGGGTGACAAAg                                                 >  2:120280/1‑141 (MQ=255)
                                                                                       cGACCAAGTTGCAGTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                <  2:860492/141‑1 (MQ=255)
                                                                                       cGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTGAGAGCACGTGTGATCTCACATGCGTTAAAAGATATGCTTGCAGAGGGTGACAAAGt                                                >  1:136463/1‑141 (MQ=255)
                                                                                       cGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACACAGt                                                >  1:998862/1‑141 (MQ=255)
                                                                                       cGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                >  1:476745/1‑141 (MQ=255)
                                                                                          ccAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGCTCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCAt                                             <  2:150388/141‑1 (MQ=255)
                                                                                           cAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCAtt                                            <  1:545113/141‑1 (MQ=255)
                                                                                            aaGTTGCAATTAAAAGTATTAATGGTACGGTGCCTTTCTATGACGGTAAGACTGACCCGACGGAGAAACGTACTCGTGTAATAGCACGTGTGATCTCACATGCGTTAAAAGCTATCCTTGCTGAGGGTGACAAAGTCATTa                                           <  2:667087/141‑1 (MQ=255)
                                                                                                  cAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGGGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATgg                                     <  1:761901/141‑1 (MQ=255)
                                                                                                    aTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGa                                   >  2:752478/1‑141 (MQ=255)
                                                                                                    aTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGa                                   >  1:702097/1‑141 (MQ=255)
                                                                                                     ttAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGGTGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGGGACAAAGTCATTATCATGGGAc                                  <  2:342674/141‑1 (MQ=255)
                                                                                                      tAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCAGGGGACa                                 >  2:596715/1‑141 (MQ=255)
                                                                                                         aaGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATaa                              <  1:1184972/141‑1 (MQ=255)
                                                                                                         aaGTATTAATGGTAATGGGCGTTTCTATGGCGGTAAGCCTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATaa                              <  2:930427/141‑1 (MQ=255)
                                                                                                            tATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGCAACGTACGCTTGGAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACg                           >  1:1123648/1‑141 (MQ=255)
                                                                                                             aTTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACGTGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGt                          >  2:789661/1‑141 (MQ=255)
                                                                                                              ttAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTc                         >  1:802134/1‑141 (MQ=255)
                                                                                                                aaTGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCt                       >  2:223344/1‑141 (MQ=255)
                                                                                                                       aTGGGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAg                <  1:764996/141‑1 (MQ=255)
                                                                                                                         gtgCGTTTCTATGGAGGTAAGACTGACCCAATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGAt              >  1:638702/1‑141 (MQ=255)
                                                                                                                          tgCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGGTCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATg             >  2:1028930/1‑141 (MQ=255)
                                                                                                                            cGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCa           >  1:740930/1‑141 (MQ=255)
                                                                                                                            cGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCa           >  1:418399/1‑141 (MQ=255)
                                                                                                                              tttCTATGACGGTAAGACTTACCCGATGGAGAAACCTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAAt         <  1:745372/141‑1 (MQ=255)
                                                                                                                              tttCTATGACGGGAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAAt         <  1:641111/141‑1 (MQ=255)
                                                                                                                                 cTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTgg      >  2:794616/1‑141 (MQ=255)
                                                                                                                                  tATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGt     >  2:955082/1‑141 (MQ=255)
                                                                                                                                     ggCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTgca  >  1:308268/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                           
TATCGGTGTTGGTGAAGGTACTGAAAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCA  >  CP000730/19130‑19403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: