Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 37,591 A→T 66.1% intergenic (+287/‑222) USA300HOU_0028 → / → ugpQ1 hypothetical protein/glycerophosphodiester phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073037,5910AT66.1% 37.9 / 79.8 84intergenic (+287/‑222)USA300HOU_0028/ugpQ1hypothetical protein/glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (13/15);  new base T (24/31);  total (38/46)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

ATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCGACTTCTTTATTTATGATATTCATCT  >  CP000730/37466‑37730
                                                                                                                             |                                                                                                                                           
aTTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGa                                                                                                                              <  2:871941/141‑1 (MQ=255)
   gCAGATAAGAGGTACGTTACAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGaaaa                                                                                                                           <  1:763832/141‑1 (MQ=255)
        tgagagGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAg                                                                                                                      <  1:339767/139‑1 (MQ=255)
                  gTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTAc                                                                                                            >  2:443662/1‑141 (MQ=255)
                        aGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATGGAAGATAAGGGGTACAGAAAAAATAGACTTGATTACAAAATg                                                                                                      >  2:703843/1‑141 (MQ=255)
                           aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAg                                                                                                   >  1:196633/1‑141 (MQ=255)
                            gTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGc                                                                                                  <  1:961040/141‑1 (MQ=255)
                                tAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTgg                                                                                              >  2:831223/1‑141 (MQ=255)
                                      aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGAACATa                                                                                        >  2:464066/1‑141 (MQ=255)
                                            cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                                                                                  <  1:1063185/141‑1 (MQ=255)
                                            cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                                                                                  <  1:1063025/141‑1 (MQ=255)
                                             aGATAAGAGGTTTGTTAAAAGCAGTTCTAAGTAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAtt                                                                                 >  2:652303/1‑141 (MQ=17)
                                              gATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttt                                                                                <  1:767122/141‑1 (MQ=11)
                                                tAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttttt                                                                              >  2:1048001/1‑141 (MQ=14)
                                                      gtacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                                                                        <  1:532332/138‑1 (MQ=14)
                                                      gtacGTTAAAAGCAATTATAAGAAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                                                                        <  1:564560/138‑1 (MQ=17)
                                                       tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                                                       <  1:994197/139‑1 (MQ=14)
                                                       tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                                                       <  2:175433/139‑1 (MQ=14)
                                                       tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                                                       <  1:614590/139‑1 (MQ=14)
                                                       tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCCGATAAGGCGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                                                       >  2:302561/3‑141 (MQ=17)
                                                       aacGTTAAAAGCAGTTCTATGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTAAGTAAAATTGCTGATAATGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                                                       <  2:128871/139‑1 (MQ=18)
                                                         cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                                                                     <  2:618485/141‑1 (MQ=17)
                                                         cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAAGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAACACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                                                                     <  1:43204/141‑1 (MQ=17)
                                                              aaaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatgagatga                                                                <  1:669179/141‑1 (MQ=17)
                                                               aaGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTGAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTCAAAAatgagatgag                                                               <  2:817374/141‑1 (MQ=255)
                                                               aaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCGTGGGACATAAATGATTTTTTAAAAatgagatgag                                                               >  2:1125588/1‑141 (MQ=17)
                                                                 gcagtcctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                                                             <  1:391338/129‑1 (MQ=18)
                                                                 gCAGTTCTCAGTAAAATTGAGGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGATCTTGGGACATAAATGATTTTTTAAAAAtgagatgagag                                                             >  1:777342/1‑140 (MQ=255)
                                                                 gCAGTTCTCAGTAAAANTGCTGATAAAGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGGTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                                                             <  1:1079534/141‑1 (MQ=255)
                                                                 gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                                                             <  2:1066551/141‑1 (MQ=17)
                                                                 gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                                                             <  2:765921/141‑1 (MQ=17)
                                                                 gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                                                             <  1:283797/141‑1 (MQ=18)
                                                                 gCAGTTCTAGGAAAAATTACAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGAAAAAATGCAGATAAGGGGTACAGAAAAACTAGACTTGATGACAAAATCGAGCTTGGGACATAAATAAATAAAAAAAAATGAGATGAGAc                                                             <  2:336980/141‑1 (MQ=9)
                                                                  caattctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg                                                            <  2:413388/130‑1 (MQ=18)
                                                                  cAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg                                                            <  1:1137994/141‑1 (MQ=255)
                                                                   aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGt                                                           <  1:232150/141‑1 (MQ=17)
                                                                     ttctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAACATGGAGCTTGGGACATAAATGAGTTTTTAAAAAAGAGATGAGACGTAg                                                         >  1:575860/9‑141 (MQ=17)
                                                                     ttctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATGTTTTAAAAATGAGATGAGACGTAg                                                         >  1:537995/9‑141 (MQ=17)
                                                                     ttCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAg                                                         <  1:1031214/141‑1 (MQ=255)
                                                                        taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                                                      >  2:78663/6‑141 (MQ=18)
                                                                        taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                                                      <  1:701528/136‑1 (MQ=18)
                                                                        taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                                                      >  2:1140313/6‑141 (MQ=18)
                                                                         cAGTAAAATTGCTGATAAGGGGTAAGCAAAAAGCAAGCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTa                                                     >  1:576908/1‑141 (MQ=255)
                                                                         aggcaaaaTTGCAGATAAGAGGTGCGTTACAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTGTTTAAAAATGAGATGAGACGTAGATTa                                                     >  2:871345/5‑141 (MQ=21)
                                                                          ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                                                    >  1:503498/4‑141 (MQ=21)
                                                                          ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                                                    >  1:33476/4‑141 (MQ=21)
                                                                          ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                                                    <  2:350345/138‑1 (MQ=21)
                                                                          ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTACGTCCCATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                                                    <  1:388079/138‑1 (MQ=25)
                                                                          aGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGCTGAGACGTGGATTaa                                                    >  1:563831/1‑141 (MQ=255)
                                                                           gcaaaaTTGCAGATAAGAGGTGCGTTAAACGCCGTTCTCAGTAAAAGTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                                                   <  1:513331/139‑1 (MQ=21)
                                                                           gcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACTGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                                                   <  1:806925/139‑1 (MQ=21)
                                                                           gaaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                                                   <  2:83345/139‑1 (MQ=21)
                                                                              aaaTTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTcc                                                >  1:993152/1‑141 (MQ=255)
                                                                              aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTcc                                                >  2:754367/1‑141 (MQ=21)
                                                                                 ttGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATa                                             >  1:944926/1‑141 (MQ=25)
                                                                                    cagataagaggtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATc                                          >  1:607719/15‑141 (MQ=25)
                                                                                    cagATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATc                                          >  1:410778/3‑141 (MQ=25)
                                                                                     agATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTAGAAAGTCATCCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCa                                         >  1:567847/2‑141 (MQ=21)
                                                                                     agATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCa                                         >  1:462897/2‑141 (MQ=25)
                                                                                      gATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCaa                                        <  1:781819/141‑1 (MQ=25)
                                                                                        taagaggtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGAGTTGATTACAAAATGGAGCTTGGGACATAAATGAGTTTTTAAAAATGAGATGAGACGTAGATTAAAGCCATAATCAATa                                      >  2:566188/11‑141 (MQ=25)
                                                                                         aagaggtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGCTTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAc                                     <  2:764042/132‑1 (MQ=37)
                                                                                         aagaggtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAc                                     <  2:1186571/132‑1 (MQ=37)
                                                                                         aagaggtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGAAGATTAACTCCATAATCAATAc                                     >  1:215583/10‑141 (MQ=37)
                                                                                         aaGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATGAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTACATAATCAATaa                                     >  2:683203/1‑140 (MQ=255)
                                                                                         aaGGGGTAAGTTAAAAGCAATACTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAc                                     >  1:622730/1‑141 (MQ=255)
                                                                                           gaggtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACACCATAATCAATACGa                                   <  1:885974/134‑1 (MQ=37)
                                                                                            aggtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGaa                                  >  1:494309/7‑141 (MQ=37)
                                                                                              gtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGATTc                                >  1:139427/5‑141 (MQ=38)
                                                                                              gtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATc                                >  1:129521/5‑141 (MQ=38)
                                                                                              gtgcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATc                                >  1:646552/5‑141 (MQ=38)
                                                                                                gcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTa                              <  2:1090193/139‑1 (MQ=255)
                                                                                                gcgTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTa                              <  1:542410/139‑1 (MQ=38)
                                                                                                gcgTTAAAAGCAAGTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTa                              <  1:193208/139‑1 (MQ=39)
                                                                                                   ttAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCg                           <  1:305074/141‑1 (MQ=255)
                                                                                                      aaaGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCGAct                        <  1:1012562/141‑1 (MQ=255)
                                                                                                      aaaGAAATCCTAAGTAAAATTGCAGATAAGGGGGACAGAAAAACGAGACTTGATAACAAAATGGATCTTGGAACATATACGATTTTTGATAAAGGAGATGAGCCGTAGATTAACTCCATAATCAATACGAAGCTAGCGAct                        <  2:544607/141‑1 (MQ=255)
                                                                                                            aTCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCGACTTCttta                  >  1:1041257/1‑141 (MQ=255)
                                                                                                            aTCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAAGAATCTAGCGACTTCttta                  >  2:54032/1‑141 (MQ=255)
                                                                                                               ctcaGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCAATAATCACTACGAATCTATCGACTTCttaattt               >  2:678162/4‑141 (MQ=255)
                                                                                                               ctcaGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGGAGATTAACTCCATAATCAATACGAATCTATCGACTCAtttattg               >  2:473919/4‑140 (MQ=255)
                                                                                                                 aaGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCGACTTCTttatttat             <  2:761646/141‑1 (MQ=255)
                                                                                                                 aaGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCGACTTCTttatttat             <  2:929505/141‑1 (MQ=255)
                                                                                                                    tAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCGACTTCTTTATTTATGat          <  2:884869/141‑1 (MQ=255)
                                                                                                                            gaGATAAGGGGTACAGAAAAACTAGGCTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCGACTTCTTTATTTATGATATTCAtct  <  2:1085062/140‑1 (MQ=255)
                                                                                                                             |                                                                                                                                           
ATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATACGAATCTATCGACTTCTTTATTTATGATATTCATCT  >  CP000730/37466‑37730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: