Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 588,312 A→G 3.2% E739G (GAG→GGG)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730588,3120AG 3.2% 516.4 / 2.6 163E739G (GAG→GGG) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (83/74);  new base G (3/3);  total (86/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

CGTCTAGTTGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGAAAGACTTGTGAAAGATGACG  >  CP000730/588173‑588449
                                                                                                                                           |                                                                                                                                         
cgtcTAGTTGAAGAGAACGGCGTTGAGCAGGAAGGTGAATCAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACgag                                                                                                                                          <  1:25714/141‑1 (MQ=255)
   cTAGTTGAAGAGAACGGCGTTGAGCATGCAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCACTCGGGCTTGGTGTTGAGTATAACGAGAtt                                                                                                                                       >  2:836285/1‑141 (MQ=255)
    tAGTTGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTGCATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGGATAACGAGAttt                                                                                                                                      >  1:897302/1‑141 (MQ=255)
    tAGTTGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGAttt                                                                                                                                      <  1:1075814/141‑1 (MQ=255)
    tAGTTGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGAttt                                                                                                                                      >  2:1154085/1‑141 (MQ=255)
     aGTTGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGAtttt                                                                                                                                     >  1:541771/1‑141 (MQ=255)
     aGTTGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGAtttt                                                                                                                                     <  2:10884/141‑1 (MQ=255)
      gTTGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTa                                                                                                                                    >  1:98592/1‑141 (MQ=255)
        tGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGc                                                                                                                                  <  2:950644/141‑1 (MQ=255)
         gAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCa                                                                                                                                 <  1:156654/141‑1 (MQ=255)
          aaGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAg                                                                                                                                <  1:153213/141‑1 (MQ=255)
          aaGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAg                                                                                                                                <  1:946126/141‑1 (MQ=255)
           agagAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGa                                                                                                                               <  1:1038379/141‑1 (MQ=255)
           agagAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGa                                                                                                                               <  1:752806/141‑1 (MQ=255)
           agagAACGGCGGTGAGCATGAAGGTGAATTAGATCGCTATCCAGTATGTAAATTTAAACGTTCAAACCCAGGTACATGTTACAACCAACCTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGa                                                                                                                               <  2:457929/141‑1 (MQ=255)
              gaACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATgg                                                                                                                            <  2:1143748/141‑1 (MQ=255)
               aaCGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGGGATATTAGCAGATgaa                                                                                                                           >  1:350321/1‑139 (MQ=255)
                ccGGCGTTTATCATGCAGGGGCATTAGATCGCTATCCATTAGCTAAATGTACACGTTCTAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGATATGTTGTTGAGCATATCGAGATTTTAGCAGATGGAc                                                                                                                          <  2:43335/140‑1 (MQ=255)
                aCGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGAc                                                                                                                          >  2:140448/1‑141 (MQ=255)
                aCGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCAATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGAc                                                                                                                          >  2:1091457/1‑141 (MQ=255)
                aCGGCGTTGAGCATGAAGGTGAATTAGATCACTATCCATTAGCTAAATTTAAACGTTCAACCCCCGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTCGCAGCTGGAc                                                                                                                          >  2:1152283/1‑141 (MQ=255)
                aCGGCGGTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTGAAACGCAGGTACATGTTACCACCAACGTCCAATCGTTGCAGTGGGCGCTGTTGTTGAGTGTAACTAGATTTTAGCGGAGGGAc                                                                                                                          >  1:491604/1‑141 (MQ=255)
                  ggCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATACCGAGATTTTAGCAGATGGACCa                                                                                                                        >  2:705921/1‑141 (MQ=255)
                    cGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCCTc                                                                                                                      >  2:519818/1‑141 (MQ=255)
                     gTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCACTCGTTGCAGTTGGAGCGGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCt                                                                                                                     >  2:499663/1‑141 (MQ=255)
                      ttGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTa                                                                                                                    <  1:990590/141‑1 (MQ=255)
                      ttGAGCATGAAGGTGAATTAGACCGCTATCCATTAGCTAAAATTCAAAGAAGGAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTa                                                                                                                    <  1:1172332/141‑1 (MQ=255)
                            aTGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAAt                                                                                                              <  1:790919/141‑1 (MQ=255)
                            aTGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAAt                                                                                                              <  1:1011224/141‑1 (MQ=255)
                                 ggTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGTGGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGga                                                                                                         <  1:1005058/141‑1 (MQ=255)
                                 ggTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGga                                                                                                         <  1:469940/141‑1 (MQ=255)
                                  gTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGgag                                                                                                        <  1:1145271/141‑1 (MQ=255)
                                  gTGAATTAAATCGCTATCCATTAGCTAAATTTAAACTTTTAAACTCAGTTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGgag                                                                                                        <  1:109516/141‑1 (MQ=255)
                                   ggAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGgaga                                                                                                       <  2:1029066/140‑1 (MQ=255)
                                      aTTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAAt                                                                                                    <  1:11517/141‑1 (MQ=255)
                                      aTTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAAt                                                                                                    <  1:378715/141‑1 (MQ=255)
                                      aTTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAAt                                                                                                    <  2:286332/141‑1 (MQ=255)
                                       ttAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGCTTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATg                                                                                                   >  2:170442/1‑141 (MQ=255)
                                         aGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGc                                                                                                 <  2:924681/141‑1 (MQ=255)
                                         aGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCAATGGAATTAGGAGAAATGGc                                                                                                 >  2:460346/1‑141 (MQ=255)
                                         aGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTACAGTCGTTGCAGTTGGAGATGTTGTTGAGTATGACGAGATTTTAGAAGATGGACCATATAGGGAATTAGGAGGATTgga                                                                                                 >  1:39670/1‑140 (MQ=255)
                                          gATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCGTCTATGGAATTAGGAGAAATGGCa                                                                                                >  2:1160577/1‑141 (MQ=255)
                                          gATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGcc                                                                                                >  1:838668/1‑140 (MQ=255)
                                           aTCTCTCTCCATTAGCTAAATTTAAACGTCCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCAt                                                                                               <  1:683775/141‑1 (MQ=255)
                                           aTCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGAGTTTAGCAGTTGGACCATCTATGGAAGTAGGAGAAATGGcgt                                                                                               >  1:535346/1‑139 (MQ=255)
                                            tCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCAtt                                                                                              >  2:745737/1‑141 (MQ=255)
                                              gCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAg                                                                                            <  1:18984/141‑1 (MQ=255)
                                              gCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAg                                                                                            >  2:466072/1‑141 (MQ=255)
                                               cacaccATTAGCTAAATTTAAACGTTCCAACTCAGGTACATGGTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTACGAGAAATGGCATTAgg                                                                                           <  1:594169/137‑1 (MQ=255)
                                               cTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAgg                                                                                           <  1:962444/141‑1 (MQ=255)
                                                tATCCATTAGCTAAATTTAAACGTTCAAACGCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGt                                                                                          <  2:153873/141‑1 (MQ=255)
                                                 aTCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTa                                                                                         <  1:140448/141‑1 (MQ=255)
                                                 aTCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTa                                                                                         >  1:1159789/1‑141 (MQ=255)
                                                    cATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGaa                                                                                      >  2:357772/1‑141 (MQ=255)
                                                         gCTAAATTTAAACGTTCAAACTCAGGTACATGTTACCACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGCAACAATGGCATTAGGTAGAAACgta                                                                                 >  2:537266/1‑141 (MQ=255)
                                                          cTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGCCTTTCGACGAAAGGGCATTAGGTAGAAACgtag                                                                                <  1:577968/141‑1 (MQ=255)
                                                             aaTTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGtagtag                                                                             >  1:442573/1‑141 (MQ=255)
                                                                 tAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTgg                                                                         >  2:116894/1‑141 (MQ=255)
                                                                 tAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGAAGTAGTAgg                                                                         >  2:116820/1‑141 (MQ=255)
                                                                 tAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGGTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGCCCATCTATGGAATTAGGGGAAATGGCATTAGGTAGAAACGAAGTAGTAgg                                                                         >  1:291059/1‑141 (MQ=255)
                                                                 tAAACGTTCAAAATCAGGTACATGTTAAAACCAACGTCTAATCGTTGCAGTTGGAGATGTGGTTTAGTATAACGAGAGTGTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTgg                                                                         >  2:827622/1‑141 (MQ=255)
                                                                   aaCGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGCTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATAAGGTAGAAACGTAGTAGTTGGtt                                                                       <  2:856912/141‑1 (MQ=255)
                                                                    aCGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGttt                                                                      >  2:1195638/1‑141 (MQ=255)
                                                                    aCGTTCAAACTCAGGTACATGTTACAACCAACGTCAAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATCAGGTAGAAACGTAGTAGTTGGttt                                                                      >  1:671420/1‑141 (MQ=255)
                                                                       ttCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCAt                                                                   >  2:706854/1‑141 (MQ=255)
                                                                        tCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAACTGGCATTAGGTAGAACCGTAGTAGTTGGGTTCATg                                                                  >  2:841984/1‑141 (MQ=255)
                                                                             cTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGCAACGTAGTAGTTGGTTTCATGACTTg                                                             >  2:125960/1‑141 (MQ=255)
                                                                             cTCAGGAACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTg                                                             <  1:631623/141‑1 (MQ=255)
                                                                                aGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGa                                                          <  1:792531/141‑1 (MQ=255)
                                                                                    aCATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGGAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGt                                                      >  2:470604/1‑141 (MQ=255)
                                                                                    aCATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGt                                                      <  1:745455/141‑1 (MQ=255)
                                                                                    aCATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGt                                                      <  1:1186315/141‑1 (MQ=255)
                                                                                    aCATGTTACAACCAACCTCCAATCGTTTCAGTTGGAGATGTTGTTGAGTATAACGACATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGt                                                      <  1:977789/141‑1 (MQ=255)
                                                                                      aTGTTATAACCAACGTCCCATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATTGACCATCTTTGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTTATGTCTTGGGACGGTTa                                                    <  1:579866/141‑1 (MQ=255)
                                                                                      aTGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTa                                                    <  1:558834/141‑1 (MQ=255)
                                                                                      aTGTTACAACCAACGTCCAATCCTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTATAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTa                                                    <  1:829854/141‑1 (MQ=255)
                                                                                      aTGTTACAACAAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTa                                                    <  2:416931/141‑1 (MQ=255)
                                                                                       tGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTAc                                                   >  1:50212/1‑141 (MQ=255)
                                                                                          tACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGGGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAAc                                                <  2:496731/141‑1 (MQ=255)
                                                                                              accaacGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATg                                            <  1:1195638/141‑1 (MQ=255)
                                                                                                 aacGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAgg                                         >  1:436227/1‑141 (MQ=255)
                                                                                                  acGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAAcagagagga                                        >  2:536118/1‑133 (MQ=255)
                                                                                                  acGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGa                                        >  1:1052482/1‑141 (MQ=255)
                                                                                                   cGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGAt                                       <  2:51747/141‑1 (MQ=255)
                                                                                                    gTCCGATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATAGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACGTGGGACGGTTACAACTCTGCGGAtc                                      >  2:730629/1‑140 (MQ=255)
                                                                                                    gTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAGCGTAGTAGTTGGTTTCAGGACTTGGGCCGGTTACAACCATGCGGATg                                      >  2:29339/1‑141 (MQ=255)
                                                                                                      ccAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAGCTATGAGGATGcc                                    >  1:737550/1‑141 (MQ=255)
                                                                                                            gTTGCAGGTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATc                              <  1:239858/141‑1 (MQ=255)
                                                                                                              tGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGAGGCCGTTATCAt                            >  2:569311/1‑141 (MQ=255)
                                                                                                                cAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATgg                          >  1:633379/1‑140 (MQ=255)
                                                                                                                 aGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAg                         <  2:677620/141‑1 (MQ=255)
                                                                                                                 aGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAg                         >  2:992802/1‑141 (MQ=255)
                                                                                                                      gagaTGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGaaa                    >  1:314933/1‑141 (MQ=255)
                                                                                                                          tgttgtTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGAAAGACt                >  1:1065069/1‑141 (MQ=255)
                                                                                                                           gttgttGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGAAAGACtt               <  2:649286/141‑1 (MQ=255)
                                                                                                                                  aGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAAAGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGGAAGCCTTGTGAAAg        >  2:264928/1‑141 (MQ=255)
                                                                                                                                   gTATAACGGGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGAAAGACTTGTGAAAGa       <  1:721987/141‑1 (MQ=255)
                                                                                                                                    tataACGAGATTTTAGCAGATGGACCATCTATGGGATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTGTACGGATGCCGGTATCATTAGTGAAAgcgtgatgaaacat      >  2:628821/1‑128 (MQ=255)
                                                                                                                                     ataACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGAAAGACTTGTGAAAGATg     >  2:406156/1‑141 (MQ=255)
                                                                                                                                        aCGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGAAAGACTTGTGAACGATGACg  >  1:376626/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CGTCTAGTTGAAGAGAACGGCGTTGAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGTACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATTTTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTTGGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGAAAGACTTGTGAAAGATGACG  >  CP000730/588173‑588449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: