Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 665,392 T→G 19.4% L79* (TTA→TGA)  USA300HOU_0608 → possible acetytransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730665,3920TG19.4% 243.9 / 64.8 132L79* (TTA→TGA) USA300HOU_0608possible acetytransferase
Reads supporting (aligned to +/- strand):  ref base T (55/49);  new base G (14/11);  total (70/62)
Fisher's exact test for biased strand distribution p-value = 8.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

Only 100 of 135 total aligned reads displayed.

TGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGCA  >  CP000730/665252‑665531
                                                                                                                                            |                                                                                                                                           
tGCGACAACAGTTAATCACTTGTTAGTAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTATTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAAtt                                                                                                                                             <  1:722922/141‑1 (MQ=255)
tGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAAtt                                                                                                                                             <  2:1174191/141‑1 (MQ=255)
    acaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCGTTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGatat                                                                                                                                         >  1:919363/1‑141 (MQ=255)
    acaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTatat                                                                                                                                         <  1:1119158/141‑1 (MQ=255)
    aaagcaGTTAATAACTTGTTAGAAGCTTCATCTAATGACCATCATTTAAAGAAAAGACTCCAAGAACAATTTTTCTTAATCGTTGACGAAGGTAATGACATAGTTGGCTTTGCTAACTGTATTTACGGTGATGAATTatat                                                                                                                                         >  2:322714/5‑141 (MQ=255)
       acaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTa                                                                                                                                      >  1:218292/1‑141 (MQ=255)
       acaGTTAATCACTTGTTAGAAGCTTCATATAATAAACATCATTTAACGAAAAGACTTCAATAACAATTATTCTTAGTCGTTGAAGAAGGTCATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATAAGATTTa                                                                                                                                      >  2:160726/1‑141 (MQ=255)
           ttAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAg                                                                                                                                  >  1:588460/1‑141 (MQ=255)
           ttAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAg                                                                                                                                  >  2:403772/1‑141 (MQ=255)
            tAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTGTATTTATCAGc                                                                                                                                 >  1:60852/1‑141 (MQ=255)
                cACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTAAGGTGAAGAATTATATTTATCAGCTCAt                                                                                                                             <  2:698939/141‑1 (MQ=255)
                cACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTAGATAACTTTATACACGGTGAAGAATAATAATTATCATCTCAt                                                                                                                             <  2:698972/141‑1 (MQ=255)
                  cTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATGTACGGTGAAGAAGTATATTTCTCAGCTCAttc                                                                                                                           >  1:492958/1‑140 (MQ=255)
                   ttGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTAt                                                                                                                          <  2:1084871/141‑1 (MQ=255)
                   ttGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTAt                                                                                                                          <  2:1084997/141‑1 (MQ=255)
                    tGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTCATCAAACAGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATg                                                                                                                         >  1:1176231/1‑141 (MQ=255)
                    tGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATg                                                                                                                         >  2:725322/1‑141 (MQ=255)
                     gTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTTTGt                                                                                                                        >  2:63656/1‑141 (MQ=255)
                     gTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGt                                                                                                                        <  2:740777/141‑1 (MQ=255)
                      ttAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGtt                                                                                                                       <  1:1021422/141‑1 (MQ=255)
                      ttAGAAGCTTCATAATATGAACATCATTTAAAGAAAAGACTTCAAGCACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGtt                                                                                                                       <  1:1021418/141‑1 (MQ=255)
                        aGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTaa                                                                                                                     >  1:785007/1‑141 (MQ=255)
                        aGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTaa                                                                                                                     <  2:800025/141‑1 (MQ=255)
                        aGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATGCTTAGTCGTGGAAGAAGGTAATGACATCGTTAGCTTTGCTAACTTTATTTACGTTGAAGAATTATCTGTATCAGATAATTATGTTaa                                                                                                                     >  2:552667/1‑141 (MQ=255)
                         gAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTaaa                                                                                                                    >  1:856694/1‑141 (MQ=255)
                         gAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTaaa                                                                                                                    >  1:968969/1‑141 (MQ=255)
                          aaGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGATCAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAAc                                                                                                                   >  2:1092917/1‑141 (MQ=255)
                           aGCTTCATATAATTAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAcc                                                                                                                  <  1:244739/141‑1 (MQ=255)
                            gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCa                                                                                                                 >  1:928113/1‑141 (MQ=255)
                            gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCa                                                                                                                 <  1:1105594/141‑1 (MQ=255)
                            gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATAATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCa                                                                                                                 <  2:1029309/141‑1 (MQ=255)
                               tCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGaa                                                                                                              <  2:106353/141‑1 (MQ=255)
                                cATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAAt                                                                                                             <  2:861580/141‑1 (MQ=255)
                                 atatAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATc                                                                                                            >  1:316469/1‑141 (MQ=255)
                                   ataATGAACATCATTTAAAGTAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                          >  1:725801/1‑141 (MQ=255)
                                   ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                          <  2:1055336/141‑1 (MQ=255)
                                   ataAGGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                          <  2:325158/141‑1 (MQ=255)
                                       tGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACa                                                                                                      >  2:450666/1‑141 (MQ=255)
                                        gAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAAAATCGCAACAt                                                                                                     >  2:156752/1‑141 (MQ=255)
                                            atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAg                                                                                                 >  2:249883/1‑141 (MQ=255)
                                            atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCCAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAg                                                                                                 <  2:1025469/141‑1 (MQ=255)
                                             tcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTAGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCCACAtcacgg                                                                                                >  2:225845/1‑136 (MQ=255)
                                               atTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGtt                                                                                              <  1:977184/141‑1 (MQ=255)
                                                 ttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTAt                                                                                            >  2:572797/1‑141 (MQ=255)
                                                 ttAAAGAAAAGACTTAAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTAt                                                                                            >  1:885882/1‑141 (MQ=255)
                                                    aagaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGt                                                                                         <  1:787218/141‑1 (MQ=255)
                                                      gaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAc                                                                                       >  1:368658/1‑141 (MQ=255)
                                                      aaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACCTCGTGGGCGTTGCTAACTTTATTTACGGCGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAc                                                                                       <  1:928667/140‑1 (MQ=255)
                                                        aaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAGGAATTATATGTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAg                                                                                     >  2:542886/1‑141 (MQ=255)
                                                            aCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGAAtt                                                                                 >  2:885487/1‑141 (MQ=255)
                                                               tCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGtt                                                                              >  1:683785/1‑141 (MQ=255)
                                                               tCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGtt                                                                              >  2:1144499/1‑141 (MQ=255)
                                                                cAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTa                                                                             >  1:358185/1‑141 (MQ=255)
                                                                cAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGGACAGCATTGTTa                                                                             >  1:385928/1‑141 (MQ=255)
                                                                  aGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATTCAGGTTATGGTGCAGCATTGTTaaa                                                                           >  2:316860/1‑141 (MQ=255)
                                                                       aaTTATTCTTAGTCGTTGAAGTAGGGGATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                      >  2:1157294/1‑141 (MQ=255)
                                                                       aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                      >  2:898981/1‑141 (MQ=255)
                                                                       aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                      >  2:731070/1‑141 (MQ=255)
                                                                       aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACAGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAAAATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                      >  2:666611/1‑141 (MQ=255)
                                                                        attattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAgg                                                                     <  2:94137/141‑1 (MQ=255)
                                                                          tattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGAt                                                                   <  2:588460/141‑1 (MQ=255)
                                                                          tattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGAt                                                                   <  1:403772/141‑1 (MQ=255)
                                                                          tattCGTAGTCCTTGAAGAAGGTAATGACACCGTTGGCTTTGCTAAATCTATTTACGGTGAAGAATGATATTTATCAGCTAATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGAt                                                                   <  1:160172/141‑1 (MQ=255)
                                                                           attCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGAATTGTTAAATGAAGGAtt                                                                  >  2:228525/1‑141 (MQ=255)
                                                                                 aGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACg                                                            <  1:500277/141‑1 (MQ=255)
                                                                                       tGAAGAAGGTAATGCCATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGa                                                      >  1:782287/1‑141 (MQ=255)
                                                                                        gaagaaGGTAATGACATCGTTGGCTTTGGCAACGGTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGaa                                                     >  1:577271/1‑141 (MQ=255)
                                                                                           aaagtttaTGACATCGTTGGCTTTGCTAACTGTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAAAATACCGTTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGAt                                                  <  1:743547/134‑1 (MQ=255)
                                                                                            aagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATa                                                 >  2:66878/1‑141 (MQ=255)
                                                                                              ggttattgtaTCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATaaa                                               <  2:242403/132‑1 (MQ=255)
                                                                                              ggTAATGGCTTCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATaaa                                               <  2:951804/141‑1 (MQ=255)
                                                                                              ggTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATgaa                                               >  1:1193905/1‑141 (MQ=255)
                                                                                              ggTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATaaa                                               <  1:187125/141‑1 (MQ=255)
                                                                                                  aTGACATCGTTGGCTTTGCTAACTTTATTTAAGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAAAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttgg                                           >  2:225220/1‑139 (MQ=255)
                                                                                                   tGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGa                                          <  1:159342/141‑1 (MQ=255)
                                                                                                    gACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGaa                                         <  2:373271/141‑1 (MQ=255)
                                                                                                      cATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAgg                                       >  2:473544/1‑141 (MQ=255)
                                                                                                       aTCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGgt                                      <  1:1192038/141‑1 (MQ=255)
                                                                                                       aTCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTCTTAAATGACGGATTATCACATTTTGAAGATAAATTTGAAgtt                                      >  2:459272/1‑139 (MQ=255)
                                                                                                        tCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCCGCCCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAgggg                                     >  1:1065875/1‑139 (MQ=255)
                                                                                                         cGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGgtgt                                    >  1:26357/1‑141 (MQ=255)
                                                                                                          gTTGGCTTTGCTAACTTTATTTAGGGTTAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAAAATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGGTAAATTGGAAggagat                                   >  2:39489/1‑137 (MQ=255)
                                                                                                          gTTGGCTTTGCTAACGTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGATAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGtt                                   <  2:639189/141‑1 (MQ=255)
                                                                                                           ttGGCTTTGCTAAATTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAAACGCAAAATACAGGTTATGGTACAGCATTGTTAAAAGAAGGATTATCGCGTTTTGAAGATAAATTTGAACGAGttt                                  >  2:702294/1‑141 (MQ=255)
                                                                                                               cTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGCTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACtt                              <  1:356520/141‑1 (MQ=255)
                                                                                                               cTTTGCTAACTTTATTGACGGTGCAGAATGATATTTATCAGCTCATCATGTTAAACCAGAATCGAAACATACAGGTTATGGTAGAGCATTGTGAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACtt                              <  1:258968/141‑1 (MQ=255)
                                                                                                                   gCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACAAACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGaa                          >  2:783796/1‑141 (MQ=255)
                                                                                                                    cTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAg                         >  1:333461/1‑141 (MQ=255)
                                                                                                                       aCTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAg                      <  1:542886/141‑1 (MQ=255)
                                                                                                                        atttatttaCGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGAAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGa                     <  2:1054479/140‑1 (MQ=255)
                                                                                                                         tttatttaCGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGat                    >  2:1043703/1‑141 (MQ=255)
                                                                                                                         gttatttatGGTCAAGTATTATATATATCAGCTCAGTATGTTAAACCAGAATCGCAACATACAGGTTATGGGACAGCATTTTTAAATGAAGGATTATCACGTTGTGAAAATAAATTTGAAGGTGTTTACTTAGAAGTAGat                    <  2:562026/140‑1 (MQ=255)
                                                                                                                               taCGGGGAGGTATTGTATTTATGAGCGCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGCTTAACGAGTAGGGTATGTTTAGTTGAAGGTGTTTACTTAGAAGTAGATAATaaa              >  2:539926/1‑141 (MQ=255)
                                                                                                                                  ggTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAAt           >  1:948846/1‑141 (MQ=255)
                                                                                                                                     gaagaaTTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaa        <  2:26357/141‑1 (MQ=255)
                                                                                                                                       agaaTTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaaga      >  2:772905/1‑141 (MQ=255)
                                                                                                                                        gaaTTATATTTATCAGCTCATTATGTTAAGCCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGGTAATAAAAATgaagaa     <  1:592095/141‑1 (MQ=255)
                                                                                                                                        gaaTTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaagaa     <  1:341304/141‑1 (MQ=255)
                                                                                                                                         aaTTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGGTAATAAAAATGaagaag    >  1:439467/1‑141 (MQ=255)
                                                                                                                                          aTTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGc   <  2:634695/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TGCGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAAGCA  >  CP000730/665252‑665531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: