Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 37,539 C→A 21.5% intergenic (+235/‑274) USA300HOU_0028 → / → ugpQ1 hypothetical protein/glycerophosphodiester phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073037,5390CA21.5% 200.0 / 35.9 91intergenic (+235/‑274)USA300HOU_0028/ugpQ1hypothetical protein/glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (37/34);  new base A (5/15);  total (42/49)
Fisher's exact test for biased strand distribution p-value = 4.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACGTTAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTA  >  CP000730/37400‑37679
                                                                                                                                           |                                                                                                                                            
gTACGTTAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCa                                                                                                                                             >  2:994197/1‑141 (MQ=255)
 tACGTTAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAg                                                                                                                                            <  2:940295/141‑1 (MQ=255)
 tACGTTAAAAGCAATTCCATGCAAAATTGCTCAGAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAg                                                                                                                                            <  2:670184/141‑1 (MQ=255)
 tACGTTAAAAGCAATTCCATGCAAAATAGCTGATAAGGGATAAGTTATAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAg                                                                                                                                            <  1:811018/141‑1 (MQ=255)
  aCGTTAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAga                                                                                                                                           >  1:246695/1‑140 (MQ=255)
  aCGTTAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGt                                                                                                                                           <  1:609245/141‑1 (MQ=255)
  aCGTTAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGt                                                                                                                                           <  1:310077/141‑1 (MQ=255)
     ttAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTaaa                                                                                                                                        >  1:790479/1‑141 (MQ=255)
     ttAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTaaa                                                                                                                                        >  1:575940/1‑141 (MQ=255)
     ttAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTaaa                                                                                                                                        <  2:712367/141‑1 (MQ=255)
      tAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAAGTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTaaaa                                                                                                                                       >  2:885974/1‑141 (MQ=255)
      tAAAAGCAATTCCATGCAAAATTGCTGAGAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTaaaa                                                                                                                                       >  1:543426/1‑141 (MQ=255)
       aaaaGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAAt                                                                                                                                      <  1:1133372/141‑1 (MQ=255)
       aaaaGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAAt                                                                                                                                      >  2:767122/1‑141 (MQ=255)
        aaaGCAATTGCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCACTCCTAGCACAACTTGCAGATAAGAGGGGCGTTAAAAGCAGTTCTCAGTAAAAtt                                                                                                                                     >  2:50373/1‑141 (MQ=18)
        aaaGCAATTCCAAGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAAtt                                                                                                                                     <  2:316786/141‑1 (MQ=255)
         aaGCAATTCCATGCAAAATTGCTGATAAGGTGTTAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTg                                                                                                                                    <  1:740488/141‑1 (MQ=255)
         aaGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTg                                                                                                                                    <  1:1133514/141‑1 (MQ=255)
           gCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCt                                                                                                                                  <  1:35356/141‑1 (MQ=255)
             aatgttaTGCAAAATCTCAGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGa                                                                                                                                <  2:614004/135‑1 (MQ=17)
              aTTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAt                                                                                                                               <  1:1076871/141‑1 (MQ=255)
              aTTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAt                                                                                                                               >  1:364913/1‑141 (MQ=255)
              aTTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAt                                                                                                                               <  2:522110/141‑1 (MQ=255)
              aTTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAt                                                                                                                               >  1:442219/1‑141 (MQ=255)
              aTTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAt                                                                                                                               <  1:463268/141‑1 (MQ=255)
              aTTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAt                                                                                                                               <  1:734315/141‑1 (MQ=255)
              aTTCCAAGCAAAATTGCAGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAt                                                                                                                               <  2:669413/141‑1 (MQ=255)
               ttCCATGCAAAATTGCTGATAAGGGGTAAGTTTAAAGCGGTTATCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCGGATAAGAGGTGCGTTCAGAGCAGTTCTCAGTAAGATTGGTGAtg                                                                                                                              >  1:464679/1‑140 (MQ=18)
               ttCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATa                                                                                                                              <  1:1032712/141‑1 (MQ=255)
                tCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATaa                                                                                                                             >  2:1031214/1‑141 (MQ=255)
                 ccATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATGAg                                                                                                                            >  2:1140745/1‑141 (MQ=255)
                 ccATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTATAAGTAAAATTGCTGATAAg                                                                                                                            >  2:626404/1‑141 (MQ=255)
                   aTGCAAGATGGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCTGTTCTCAGTAAAATTGCTGATAAgcg                                                                                                                          >  1:878264/1‑139 (MQ=255)
                   aTGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAggg                                                                                                                          >  1:672272/1‑141 (MQ=255)
                   aTGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAggg                                                                                                                          >  1:153145/1‑141 (MQ=255)
                     gCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAAGTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTAAAAATCAGTTCTCAGTAAAATTGCTGATAAggggg                                                                                                                        >  2:763832/1‑140 (MQ=255)
                      cAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTa                                                                                                                       >  1:625281/1‑141 (MQ=255)
                          aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacaga                                                                                                                   >  1:486021/1‑137 (MQ=255)
                          aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacaga                                                                                                                   <  1:479232/141‑5 (MQ=255)
                          aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATGGCGGATAAGGGGTacaga                                                                                                                   >  2:353603/1‑137 (MQ=255)
                          aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGAAGATAAGAGTAGCGTTAAAAGCCGTCCTCAATAAAATTGCGGATAAgggagaaggt                                                                                                                   >  2:1079534/1‑134 (MQ=18)
                           ttGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAGAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATGGCTGATAAGGGGTacagaa                                                                                                                  >  1:6247/1‑136 (MQ=255)
                           ttGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAgcggaacagaa                                                                                                                  >  1:5942/1‑134 (MQ=255)
                           ttGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaa                                                                                                                  >  1:1121189/1‑136 (MQ=255)
                            tGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagcaa                                                                                                                 >  2:388079/1‑135 (MQ=255)
                            tGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaa                                                                                                                 >  1:350345/1‑135 (MQ=255)
                              cagagaaGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                               <  1:544203/136‑9 (MQ=255)
                              cTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGCGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                               <  2:1088932/141‑9 (MQ=255)
                              cTGATAAAGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAATGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                               <  2:858051/141‑9 (MQ=255)
                               tGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAgggggacagaaaaac                                                                                                              >  2:920047/1‑130 (MQ=255)
                                gATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGAAGTTCTGGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAGAAGGGGCAAGTTAAAAga                                                                                                             >  2:1131275/1‑140 (MQ=255)
                                   aaGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCGGATAAGGGGTacagaaaaactaga                                                                                                          >  1:1110228/1‑128 (MQ=18)
                                      gggTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATCAGCGGTGCGCGAAAACCATGTCGCAGGAAAATTGCTGATAAGGGGTAAGTTAATAGCAATcaa                                                                                                       >  2:542855/1‑139 (MQ=18)
                                          aaGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGAACGTTGAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGt                                                                                                   <  1:924110/141‑1 (MQ=255)
                                                 aaGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTg                                                                                            >  1:1085062/1‑141 (MQ=255)
                                                  aGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGc                                                                                           <  2:148679/141‑1 (MQ=255)
                                                                  aTTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGa                                                                           <  2:871941/141‑1 (MQ=255)
                                                                     gCAGATAAGAGGTACGTTACAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGaaaa                                                                        <  1:763832/141‑1 (MQ=255)
                                                                          tgagagGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAg                                                                   <  1:339767/139‑1 (MQ=255)
                                                                                    gTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTAc                                                         >  2:443662/1‑141 (MQ=255)
                                                                                          aGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATGGAAGATAAGGGGTACAGAAAAAATAGACTTGATTACAAAATg                                                   >  2:703843/1‑141 (MQ=255)
                                                                                             aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAg                                                >  1:196633/1‑141 (MQ=255)
                                                                                              gTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGc                                               <  1:961040/141‑1 (MQ=255)
                                                                                                  tAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTgg                                           >  2:831223/1‑141 (MQ=255)
                                                                                                        aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGAACATa                                     >  2:464066/1‑141 (MQ=255)
                                                                                                              cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                               <  1:1063185/141‑1 (MQ=255)
                                                                                                              cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                               <  1:1063025/141‑1 (MQ=255)
                                                                                                               aGATAAGAGGTTTGTTAAAAGCAGTTCTAAGTAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAtt                              >  2:652303/1‑141 (MQ=17)
                                                                                                                gATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttt                             <  1:767122/141‑1 (MQ=11)
                                                                                                                  tAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttttt                           >  2:1048001/1‑141 (MQ=14)
                                                                                                                        gtacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                     <  1:532332/138‑1 (MQ=14)
                                                                                                                        gtacGTTAAAAGCAATTATAAGAAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                     <  1:564560/138‑1 (MQ=17)
                                                                                                                         tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                    <  2:175433/139‑1 (MQ=14)
                                                                                                                         tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                    <  1:994197/139‑1 (MQ=14)
                                                                                                                         tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                    <  1:614590/139‑1 (MQ=14)
                                                                                                                         tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCCGATAAGGCGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                    >  2:302561/3‑141 (MQ=17)
                                                                                                                         aacGTTAAAAGCAGTTCTATGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTAAGTAAAATTGCTGATAATGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                    <  2:128871/139‑1 (MQ=18)
                                                                                                                           cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                  <  2:618485/141‑1 (MQ=17)
                                                                                                                           cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAAGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAACACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                  <  1:43204/141‑1 (MQ=17)
                                                                                                                                aaaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatgagatga             <  1:669179/141‑1 (MQ=17)
                                                                                                                                 aaGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTGAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTCAAAAatgagatgag            <  2:817374/141‑1 (MQ=255)
                                                                                                                                 aaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCGTGGGACATAAATGATTTTTTAAAAatgagatgag            >  2:1125588/1‑141 (MQ=17)
                                                                                                                                   gCAGTTCTCAGTAAAATTGAGGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGATCTTGGGACATAAATGATTTTTTAAAAAtgagatgagag          >  1:777342/1‑140 (MQ=255)
                                                                                                                                   gCAGTTCTCAGTAAAANTGCTGATAAAGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGGTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc          <  1:1079534/141‑1 (MQ=255)
                                                                                                                                   gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc          <  2:765921/141‑1 (MQ=17)
                                                                                                                                   gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc          <  1:283797/141‑1 (MQ=18)
                                                                                                                                   gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc          <  2:1066551/141‑1 (MQ=17)
                                                                                                                                   gCAGTTCTAGGAAAAATTACAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGAAAAAATGCAGATAAGGGGTACAGAAAAACTAGACTTGATGACAAAATCGAGCTTGGGACATAAATAAATAAAAAAAAATGAGATGAGAc          <  2:336980/141‑1 (MQ=9)
                                                                                                                                    cAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg         <  1:1137994/141‑1 (MQ=255)
                                                                                                                                     aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGt        <  1:232150/141‑1 (MQ=17)
                                                                                                                                       ttCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAg      <  1:1031214/141‑1 (MQ=255)
                                                                                                                                           cAGTAAAATTGCTGATAAGGGGTAAGCAAAAAGCAAGCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTa  >  1:576908/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GTACGTTAAAAGCAATTCCATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTA  >  CP000730/37400‑37679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: