Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 772,803 C→A 6.1% A18E (GCA→GAA)  fruA → PTS family fructose/mannitol (fru) porter component IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730772,8030CA 6.1% 397.6 / 2.1 130A18E (GCA→GAA) fruAPTS family fructose/mannitol (fru) porter component IIABC
Reads supporting (aligned to +/- strand):  ref base C (61/58);  new base A (3/8);  total (64/66)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TTGATGAGGACTTAGCAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAGGTATTGCC  >  CP000730/772666‑772942
                                                                                                                                         |                                                                                                                                            
ttGATGAGGACTTAGAAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCaaa                                                                                                                                           <  1:14934/141‑1 (MQ=255)
   atgaGGACTTAGCAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGa                                                                                                                                        >  1:1164667/1‑141 (MQ=255)
   atgaGGACTTAGCAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGa                                                                                                                                        >  1:986573/1‑141 (MQ=255)
   atgaGGACTTAGCAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGa                                                                                                                                        <  2:1083688/141‑1 (MQ=255)
       ggACTTAGCAACACGGGACGCTACAGAAACAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGCGAGTAACAGAGATATTAACAAAAGATACCATAGCACTGGATTTAAAGGCAAACGACaaa                                                                                                                                   >  2:265504/1‑141 (MQ=255)
          cTTAGCAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAAt                                                                                                                                <  2:959669/141‑1 (MQ=255)
             aGCAACACGGGACGCAATAGAAAAAATAAAACCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGgt                                                                                                                             <  1:1164970/141‑1 (MQ=255)
               cAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGgtgt                                                                                                                           <  1:992396/141‑1 (MQ=255)
               cAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAAGGgcgt                                                                                                                           >  1:264147/1‑141 (MQ=255)
               aaaCACGGGACGCTAAAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGAGTTAATGGCAAATGACAAAAATGgtgt                                                                                                                           <  2:146492/140‑1 (MQ=255)
                 acaaGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAGCAAAAGATACAATAGCAATGGATTTAAAGGCAAAGGACAAAAATGGTGTTa                                                                                                                         <  2:850279/141‑1 (MQ=255)
                    cGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTGGCGGACTAGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAGCAAAAGAGACAATAGCAATGGATTTAGTAGAAAATACAGAAAATGGGGTTATtg                                                                                                                      >  2:186404/1‑141 (MQ=255)
                    cGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATtg                                                                                                                      >  2:556877/1‑141 (MQ=255)
                     gggACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATtga                                                                                                                     <  1:345313/141‑1 (MQ=255)
                     gggACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATtga                                                                                                                     <  1:828179/141‑1 (MQ=255)
                     gggACGCAATAGAAAAAATAAAATCACGAGTTACGATTGGCGAACTTGATGAGGAGTGAGATTAATGTGAGTGACACAATTATGAACAAAAGCTCTCAGAGGATTGGATTGAATGGCGAATGACAAAGATGGGGTTGTtga                                                                                                                     <  2:508330/141‑1 (MQ=255)
                      ggACGCAATAGAAAAAATAAAATCACAAGTTACGATGAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAAAAAAAAAAAAAAAAAATGGTGTTATtgct                                                                                                                    <  2:309808/141‑3 (MQ=255)
                       aaCGCTAAAGAAAAAATAAAATCAAAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATtgatg                                                                                                                   <  1:721205/140‑1 (MQ=255)
                        aCGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACCAGATGGGGAGGGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATtgataa                                                                                                                  >  2:703486/1‑139 (MQ=255)
                         cGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAg                                                                                                                 >  1:998494/1‑141 (MQ=255)
                         agaaaaaaaaaaaaaaaaATCACAAGTTACGATTAGCGTACTTGAAGGGGAATGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAAAGAAAAAAAAAAAAAATGGTGTTATTGATGAg                                                                                                                 <  2:415068/133‑1 (MQ=255)
                           cTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGtt                                                                                                               <  1:833966/141‑1 (MQ=255)
                           cTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGtt                                                                                                               <  1:946937/141‑1 (MQ=255)
                            tataGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGAAAAAAACAAAAATGGTGTTATTGATGAGTTa                                                                                                              <  2:753305/141‑1 (MQ=255)
                             ataGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAATATGGCGTTATTGATGAGTTAg                                                                                                             <  1:611117/141‑1 (MQ=255)
                             ataGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAg                                                                                                             >  2:113737/1‑141 (MQ=255)
                             ataGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAg                                                                                                             >  1:1080942/1‑141 (MQ=255)
                             ataGAAAAAAGAAAATGATAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAACAATGAGAGTAAAAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAAAAAAAAAAAAAAAAAATGGTGTTATTGATGAGTTAg                                                                                                             <  2:175740/141‑1 (MQ=255)
                                 aaaaaaTAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGTTGTGCTCGTaaa                                                                                                         >  2:267120/1‑141 (MQ=255)
                                       taaaatCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAAta                                                                                                   >  1:1201626/1‑140 (MQ=255)
                                         aaatCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAg                                                                                                 >  1:262727/1‑141 (MQ=255)
                                         aaatCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAg                                                                                                 >  1:49448/1‑141 (MQ=255)
                                          aatCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGa                                                                                                >  1:1107800/1‑141 (MQ=255)
                                            tCACAAGTTACGATTAGCGTACTTGATGGGGAGAGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACa                                                                                              >  2:78599/1‑141 (MQ=255)
                                                  tttACGATTAGCGTACTGGATGGGGAGTGAAAATAATGAGAGGAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAg                                                                                        <  1:1027750/140‑1 (MQ=255)
                                                  gtgagtaTTAGCGTACTTGGTGGGGAGTGAAAATACTGAGATTAACAGTGTTATTAACAAAATATACAATAGCAAGGGATTTAATGGCAAATGACAAAAAGGGTGTTATTGATGTGCTAGTAAATCAAGTAGACAAAGCAg                                                                                        <  2:20135/135‑1 (MQ=255)
                                                   ttACGATGAGCGGACTGGATGGGGAGTGAAAATAATGAGAGTAACAGAGTCATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGCTGAGTTAGTAAATCAATTAGACAAAGCAgg                                                                                       <  2:921608/141‑1 (MQ=255)
                                                   atACGATTAGCGTACTTGATGGGGAGTGAAAAAAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAAGAAAAAAAAAAAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAgg                                                                                       <  2:1178439/140‑1 (MQ=255)
                                                    tACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGt                                                                                      <  2:957811/141‑1 (MQ=255)
                                                     aCGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAATTATTAACAAAAGATACAATAGCAATGTACTTAATGGCAAATGACAAAAAGGGTGGTATTGAGGAGTGAGTAATTCAGTTAGAAAAAGAAGGTa                                                                                     >  1:254269/1‑141 (MQ=255)
                                                       gATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGAAAAAGCAGGTaaa                                                                                   >  2:618121/1‑141 (MQ=255)
                                                             cGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGt                                                                             <  1:976648/141‑1 (MQ=255)
                                                                 cgtGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATg                                                                         <  2:799773/139‑1 (MQ=255)
                                                                 cTTGATGGGGAGGGAAAATAATGAGAGTCACAGAGTTATTAACAAAAGATGCAATAGCAATGGCTTTAAAAGAAAAAGAAAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATg                                                                         <  2:1001587/141‑1 (MQ=255)
                                                                  ttGATGGGGAGTTAAAAAAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGt                                                                        <  1:862543/141‑1 (MQ=255)
                                                                   tGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTc                                                                       <  2:263366/141‑1 (MQ=255)
                                                                     aTGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTACATTAAGTGATGTcgc                                                                     <  1:1202207/141‑1 (MQ=255)
                                                                     aTGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTcgc                                                                     <  1:82580/141‑1 (MQ=255)
                                                                     aTGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTcgc                                                                     <  2:848150/141‑1 (MQ=255)
                                                                      gggggAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTCAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTcgcg                                                                    <  1:463668/140‑1 (MQ=255)
                                                                            gTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCAttt                                                              >  1:138241/1‑141 (MQ=255)
                                                                             tGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATAGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTcgcgccgtgta                                                             >  2:890772/1‑134 (MQ=255)
                                                                             agAAAATAATGAGAGTAACAGAGTTAATAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTa                                                             <  2:415964/140‑1 (MQ=255)
                                                                                  ataatGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGaa                                                        >  2:321600/1‑141 (MQ=255)
                                                                                   taatGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTATTGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAAGTAAGTGATGTCGCGTCATTTACGGAAg                                                       >  1:65665/1‑141 (MQ=255)
                                                                                     atGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCg                                                     <  1:60215/141‑1 (MQ=255)
                                                                                      tGATAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGa                                                    <  1:1084520/141‑1 (MQ=255)
                                                                                      tGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGa                                                    <  2:1140100/141‑1 (MQ=255)
                                                                                       gagaGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGAt                                                   >  1:770635/1‑141 (MQ=255)
                                                                                        agagTAACAGCGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATAGTGTTATTGATGAGGTAGTAAATCAATTAGACAAAGCAGGTAAATTACGTGGAGTAGCGTCATATAAGGAGACGAta                                                  >  2:811401/1‑140 (MQ=255)
                                                                                        agagTAACAGAGTTAGTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGAtt                                                  >  1:599076/1‑141 (MQ=255)
                                                                                              aCAGCGTTATTAACAAAAGAGACAATAGCAATGGATTTAATGGCAAAAGACAAAAATGGTGTTATAGCTGCGTTAGTAAAGCAATTAGACAAAGCAGGTAAACTAAGGGATGTAGCGTCATTTCAGGAAGCGATTCACaaa                                            >  2:14757/1‑140 (MQ=255)
                                                                                              aCAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGGGTCATTTAAGGGAGCGATTCGCAAt                                            >  1:656/1‑141 (MQ=255)
                                                                                              aCAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAAt                                            <  2:290129/141‑1 (MQ=255)
                                                                                                agagTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATAAATTAGACAAAGCAGGTAAATTAAGTGATGTAGCGTCATTTAAGGAAGAGATTCACAAGCg                                          >  2:181135/1‑141 (MQ=255)
                                                                                                     tattaACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAAt                                     <  2:223150/141‑1 (MQ=255)
                                                                                                        taACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATcac                                  <  2:1164667/141‑1 (MQ=255)
                                                                                                         caCAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTAGCGTCATTTAAGGAAGCGATTCAAAATCGAGaaaacaa                                 >  2:1038281/2‑136 (MQ=255)
                                                                                                         aaCAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCACTTAAGGAAGCGATTCACAATAGAGAATcaca                                 >  2:771547/1‑141 (MQ=255)
                                                                                                           cAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACaaa                               >  2:884653/1‑141 (MQ=255)
                                                                                                              aaGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTa                            <  1:662982/141‑1 (MQ=255)
                                                                                                                gATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTTATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACa                          <  2:882047/141‑1 (MQ=255)
                                                                                                                gATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCCTTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACa                          >  1:177690/1‑141 (MQ=255)
                                                                                                                gATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACa                          <  2:736005/141‑1 (MQ=255)
                                                                                                                  tACAATAGCAATGGATTTAATGGCAAATGACAAAAAAGGTGTTATTGATGAGTTAGTCGATCAATTAGACAAAAACGCTCAATTAACTCAAATTGCATCAACCAACGAAACGATTCAGAATCGAGAATCACAAAGTACAAc                        >  2:399720/1‑141 (MQ=255)
                                                                                                                   aCAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACt                       >  1:874015/1‑141 (MQ=255)
                                                                                                                   aCAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACt                       >  2:888941/1‑141 (MQ=255)
                                                                                                                   aCAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACt                       >  2:738955/1‑141 (MQ=255)
                                                                                                                   aCAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTAATGAGGAGTTAGTAAATCAATTGGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGATACGAATCACAATCGAGAAACACAAAGTACGAca                       >  1:896581/1‑140 (MQ=255)
                                                                                                                     aaTAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTgg                     <  1:276913/141‑1 (MQ=255)
                                                                                                                      aTAGCAATGTATTTAATAAAAAAAAAAAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGt                    <  2:403906/141‑1 (MQ=255)
                                                                                                                       tAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTa                   <  2:509486/141‑1 (MQ=255)
                                                                                                                       tAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGCGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTa                   <  1:720775/141‑1 (MQ=255)
                                                                                                                        aGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTAt                  <  1:466429/141‑1 (MQ=255)
                                                                                                                        aGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTAt                  >  2:84719/1‑141 (MQ=255)
                                                                                                                         gCAATGGATTTAATGGCAAATGACAAAAACGCTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATc                 <  1:48801/141‑1 (MQ=255)
                                                                                                                              ggATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGa            <  2:689248/141‑1 (MQ=255)
                                                                                                                              ggATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGa            >  1:19619/1‑141 (MQ=255)
                                                                                                                              ggATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGAAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGtcaaagcga            >  2:587832/1‑133 (MQ=255)
                                                                                                                                aTTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGATAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAg          >  2:1148899/1‑141 (MQ=255)
                                                                                                                                 tttAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGTCGAAgg         >  2:203487/1‑141 (MQ=255)
                                                                                                                                 tttAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAgg         >  2:800421/1‑141 (MQ=255)
                                                                                                                                  ttAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGGGAATCCCAAAGTACAACTGGTATCGGCGAAGGt        >  2:176617/1‑141 (MQ=255)
                                                                                                                                   tAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGGTGTCGCGTCATTTAAGGAAGCGATTCACAATGGAGAATCACAAAGTACAACTGGTATCGGCGAAGGtg       >  2:1069853/1‑140 (MQ=255)
                                                                                                                                   tAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAGGTa       >  1:1009610/1‑141 (MQ=255)
                                                                                                                                   tAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGGATCAGCGAAGGTa       >  1:977008/1‑141 (MQ=255)
                                                                                                                                   tAATGGCAAATGACAAAAAGGGTGTTAATGATGAGTTACTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAGGTa       <  1:242210/141‑1 (MQ=255)
                                                                                                                                    aaTGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAGGTAt      >  1:349335/1‑141 (MQ=255)
                                                                                                                                       ggCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACATAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAGGTATTGc   <  2:784205/141‑1 (MQ=255)
                                                                                                                                        gCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAGGTATTGcc  <  1:714418/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
TTGATGAGGACTTAGCAACACGGGACGCTATAGAAAAAATAAAATCACAAGTTACGATTAGCGTACTTGATGGGGAGTGAAAATAATGAGAGTAACAGAGTTATTAACAAAAGATACAATAGCAATGGATTTAATGGCAAATGACAAAAATGGTGTTATTGATGAGTTAGTAAATCAATTAGACAAAGCAGGTAAATTAAGTGATGTCGCGTCATTTAAGGAAGCGATTCACAATCGAGAATCACAAAGTACAACTGGTATCGGCGAAGGTATTGCC  >  CP000730/772666‑772942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: