Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 804,673 A→T 26.3% L135I (TTA→ATA)  USA300HOU_0751 ← POT family proton (H+)‑dependent oligopeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730804,6730AT26.3% 144.5 / 49.4 83L135I (TTA→ATA) USA300HOU_0751POT family proton (H+)‑dependent oligopeptide transporter
Reads supporting (aligned to +/- strand):  ref base A (33/28);  new base T (10/12);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

AAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCT  >  CP000730/804537‑804805
                                                                                                                                        |                                                                                                                                    
aaGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCt                                                                                                                                  >  2:1135100/1‑141 (MQ=255)
aaGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATAGTTGAAATGTTTGGCTTCATGAAACCt                                                                                                                                  >  2:1069945/1‑141 (MQ=255)
aaGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACACATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCt                                                                                                                                  >  2:1070036/1‑141 (MQ=255)
     ttGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAAcc                                                                                                                             <  2:948702/141‑1 (MQ=255)
       gCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGACGTTTGTAATGTTGGGCTTCCTTATACCCGAAccca                                                                                                                           >  2:1121635/1‑139 (MQ=255)
       gCAAAATAATAGGTGATAATAATGCACCCATACTAACCGACATACAGAAAACAACAAAACCTGAAAAAATACATCAATCCTTTTCAGGCTATAAACGACCAAGTAAATTTGAATTGTTTGGCTTCATTATACCTGCACCaa                                                                                                                           >  2:400065/1‑141 (MQ=255)
                 aGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAATACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACGTGATGCATTAATGATgaa                                                                                                                 >  2:775045/1‑141 (MQ=255)
                  ggTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAAAGTTTGGCTTCATTATACCTGCACCTATAATGATgaag                                                                                                                >  1:901134/1‑141 (MQ=255)
                       taataaTGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACAt                                                                                                           >  2:1124627/1‑141 (MQ=255)
                          ttatgtcccgttatTAACTTTCATATATTAAATAACAATACCTGCCTCCTTATTTCTATCATTTTTAGGATATAATCGACCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGa                                                                                                        <  2:625205/130‑1 (MQ=255)
                          taacGCACTCATATTAACTGACATATAGAAAAAAACAAAACCTGCATCCATACGTCTATCATTTTCAGTATATAAACGGCCAACGATACTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACAttca                                                                                                        <  2:1055489/141‑3 (MQ=255)
                           aatGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGAt                                                                                                       >  2:1020072/1‑141 (MQ=255)
                            atGCACCCATATTAGCTGACGTATAGAAAGTAACAAAACCTGCATCCATACATCTATCGATATCAGGATATAAACGGCCTAAGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGCTGAAGAACGTTGagg                                                                                                      >  2:495473/1‑139 (MQ=255)
                                   cATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAAGGGCCAACGATATTTGAAATGTTGAGATTAATTAAACCTGAACCAAAAAAGATAACCAACATTGAagtggttaa                                                                                               >  1:128056/1‑136 (MQ=255)
                                      atTAACTGACATATAGTAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTGTGGCTTCATTAAACCGGATCCAATAAATAAAGAGAACCTTGTGGTGAATatgaa                                                                                            >  1:593732/1‑137 (MQ=255)
                                      atTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGcc                                                                                            <  1:178133/141‑1 (MQ=255)
                                      atTAACTGACAAATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGcc                                                                                            <  1:735321/141‑1 (MQ=255)
                                         aaCTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTAACAATATCAGGATATAAAAAGCCACCGAAATTTGAAATGTTTGGCTTCATAAAGCCTGAACCACTAATAAGGAACAACAGGGGTGTGAATGAGCCGAt                                                                                         >  2:824126/1‑141 (MQ=255)
                                           cTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTa                                                                                       >  2:113293/1‑141 (MQ=255)
                                           cTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAAAGTTTTGCTTCATTAAACAATAACCAATAAAGATGAAAAAGATTGAtgtgtagtgtctgtttt                                                                                       >  1:363239/1‑128 (MQ=255)
                                             gACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGTTATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAAt                                                                                     <  2:816900/141‑1 (MQ=255)
                                                   taGAAAATAACAAAACCTGCATTCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGCATAAGCCGATTACTGCAAAt                                                                               <  1:754793/141‑1 (MQ=255)
                                                     gAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATgg                                                                             >  2:610603/1‑141 (MQ=255)
                                                      acaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                            <  1:1167775/139‑1 (MQ=255)
                                                      aaaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                            <  1:1020072/141‑1 (MQ=255)
                                                      aaaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                            <  2:566351/141‑1 (MQ=255)
                                                      aaaaGAACAAAACCTGCAGCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                            <  2:1115595/141‑1 (MQ=255)
                                                         aTAACAAAACCTGCATCCATACGTCTATCAGGTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAAGGGTaga                                                                         >  2:547524/1‑139 (MQ=255)
                                                           aaCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCt                                                                       <  1:353680/141‑1 (MQ=255)
                                                           aaCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCt                                                                       <  1:673923/141‑1 (MQ=255)
                                                            aCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATATCTGAACAAATAGTGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCtt                                                                      >  2:1088210/1‑141 (MQ=255)
                                                            aCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCATCATTAAACCTGAACCAATAATAATGAAGAACATTGATGTCAATAAGCCCATTAATGCAAATAGTAAACta                                                                      >  2:26076/1‑140 (MQ=255)
                                                             cAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGACCATTGATGTGAGTAAGCCGATTAATGCAAATGGTAAGGTTa                                                                     >  2:1092386/1‑141 (MQ=255)
                                                             cAAAACCTGCATCCATACGTCTATCATTTTCAGGATAGAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGAGGTGAATAAACCGATTAATCCAAATGGTAAGCTTa                                                                     >  2:133953/1‑141 (MQ=255)
                                                             cAAAACCTGCATCCATACGTCTATCATTTTCAAGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATCAGCCGATTAATGCAAATGATAAGCTTa                                                                     >  2:1144414/1‑141 (MQ=255)
                                                               aaaCCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATAATTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAACTGGTAAGCTTaaa                                                                   <  2:861339/141‑1 (MQ=255)
                                                                aaCCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAAc                                                                  <  1:1091371/141‑1 (MQ=255)
                                                                   cTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTCATGCAAATGTTAAGCATAAACaaa                                                               >  2:958477/1‑141 (MQ=255)
                                                                    tGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAGGAACATTGATGTGAATAAGCGGATTAATGCAAATGGTAAGCTTAAGCaaag                                                              >  2:711272/1‑140 (MQ=255)
                                                                        tCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAAAGTTTGGCTTCATTAAACCTGAACCAAAAATAAATAAGACCATTGATGTGAAAAAGCAGACTAAAGCAAATAGTAAGCCTAAACaacttgat                                                          >  1:198217/1‑135 (MQ=255)
                                                                         ccATACGTGTAGCATTTTCCGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGTTGTGAAGAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTc                                                         <  2:776729/141‑1 (MQ=255)
                                                                         ccATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTc                                                         >  2:1068515/1‑141 (MQ=255)
                                                                            tACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTccca                                                      >  2:224973/1‑139 (MQ=255)
                                                                             ccGTCTATCATTTTCAGGATATAAACGGCCAACGATATTGGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGat                                                     <  1:133413/140‑1 (MQ=255)
                                                                               gTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGataa                                                   <  1:672703/141‑1 (MQ=255)
                                                                               gTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGataa                                                   <  1:1135100/141‑1 (MQ=255)
                                                                                      aTTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATaaa                                            <  1:420353/141‑1 (MQ=255)
                                                                                          tCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACt                                        <  1:216475/141‑1 (MQ=255)
                                                                                             ggATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCa                                     <  2:987429/141‑1 (MQ=255)
                                                                                             ggATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGcc                                     >  2:713224/1‑140 (MQ=255)
                                                                                             ggATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAAAATTGATGTGAATAAGCCGATTAATGCAAATGGTAAACTTAAACAAAAATGTCCGATCATAATAAAGTCTGCa                                     >  2:463839/1‑141 (MQ=255)
                                                                                                     aCGGCCACCGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGCGAATAAGCCCATTCCGGCAAAGTTTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCtaataa                             <  1:279746/141‑1 (MQ=255)
                                                                                                     aCGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCGATAAAGATGAAGAACATTGATGTGAATAAGCCGAATAAAGCAAATGGTAAGCTAAAACCAACATGTCCGAAAATAATAAAGACTGCACCtaataa                             >  1:720630/1‑141 (MQ=255)
                                                                                                     aCGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCtaataa                             <  1:476297/141‑1 (MQ=255)
                                                                                                       ggCCAACGGTATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  2:649509/141‑1 (MQ=255)
                                                                                                       ggCCAACGATCTTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  1:453924/141‑1 (MQ=255)
                                                                                                       ggCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  1:678520/141‑1 (MQ=255)
                                                                                                       ggCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  2:1177163/141‑1 (MQ=255)
                                                                                                       ggCCAACGATATTGGAAATTTTTGGCTACATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  2:159007/141‑1 (MQ=255)
                                                                                                       ggCAAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                           <  1:630393/141‑1 (MQ=255)
                                                                                                        gCCAACGATATTTGACATGTTTGGCTTCATTAAACCTGAACAAATAATGATGAAGAACATTGATGTGAATAAACCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTCCCCCTCATaaaat                          >  2:446216/1‑139 (MQ=255)
                                                                                                        gCCAACGATATTTGAAATGTTTGGCTTCCTTCAACCGGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGATAAGATTAAACAAATATGTCAGATAATACTAAAGACTGCACATAATAAAga                          >  2:788386/1‑140 (MQ=255)
                                                                                                         ccAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTa                         <  2:560284/141‑1 (MQ=255)
                                                                                                             cGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAgcgc                     >  1:488203/1‑141 (MQ=255)
                                                                                                             cGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGAACCTAATAAAGTAgcgc                     >  1:548178/1‑141 (MQ=255)
                                                                                                             cGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGACAATAATAAAGACTGCACCTAATAACGTAccgc                     >  2:1143778/1‑141 (MQ=255)
                                                                                                              gATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGAACCTACAAAAGTAGCGcc                    >  2:84626/1‑141 (MQ=255)
                                                                                                               atatTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCct                   >  2:107647/1‑141 (MQ=255)
                                                                                                                 ttttGAAAAGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCctct                 <  1:447715/140‑1 (MQ=255)
                                                                                                                 atTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCctct                 <  2:1006315/141‑1 (MQ=255)
                                                                                                                 atTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCctct                 <  1:1080417/141‑1 (MQ=255)
                                                                                                                  tttGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGAATGTACCTAATAAAGTAGCGCATCTa                >  1:449778/1‑141 (MQ=255)
                                                                                                                  tttGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTGAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTa                >  1:396702/1‑141 (MQ=255)
                                                                                                                    tGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTCATAAAGTAGCGACTCTAGt              >  2:1126424/1‑141 (MQ=255)
                                                                                                                     gAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCCAGTg             <  2:828398/141‑1 (MQ=255)
                                                                                                                          gTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTTTAGTGCCTGt        <  1:867113/141‑1 (MQ=255)
                                                                                                                          gTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGt        <  1:1148568/141‑1 (MQ=255)
                                                                                                                          gTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGt        <  1:623342/141‑1 (MQ=255)
                                                                                                                           tttGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTa       >  1:123793/1‑141 (MQ=255)
                                                                                                                           tttGGCTTCATTAAACATGAACCAATAATGATGAAGAACATTGATGGGAATAAGCCGATTAATGCAAATGGTAAGGTTGGGGAGTTGTGTACGATAATAAGAAAGACTGCACCTAATAAAGTGGCGCCTATAGTGCGTGTa       >  1:591759/1‑141 (MQ=255)
                                                                                                                              ggCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAAtt    >  1:971216/1‑141 (MQ=255)
                                                                                                                               gCTTCATTAAACCTGAACCAAAAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTc   <  1:113293/141‑1 (MQ=255)
                                                                                                                                cTTTATGAAGCCTGGACCAACAATGATGAACAACATTGATGGGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGTTAATAATAAAGACGGCACCTAATAAAGTAGCGCCTGTAGTGCCTGTAATTCt  <  2:263578/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
AAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCT  >  CP000730/804537‑804805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑