Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 864,745 C→G 6.6% L21V (CTA→GTA)  USA300HOU_0809 → carboxylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730864,7450CG 6.6% 286.1 / 10.4 94L21V (CTA→GTA) USA300HOU_0809carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (37/49);  new base G (4/2);  total (41/53)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01

AATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTT  >  CP000730/864606‑864885
                                                                                                                                            |                                                                                                                                            
aaTTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCACAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATtact                                                                                                                                             <  2:101458/141‑1 (MQ=255)
aaTTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATtact                                                                                                                                             <  1:177414/141‑1 (MQ=255)
    aaGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACAt                                                                                                                                         <  1:1131353/141‑1 (MQ=255)
      gAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATgg                                                                                                                                       <  1:559067/141‑1 (MQ=255)
       aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTCTTACTACATGGt                                                                                                                                      >  1:416050/1‑141 (MQ=255)
       aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATACAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTCCATGGt                                                                                                                                      <  2:751899/141‑1 (MQ=255)
         tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGttt                                                                                                                                    <  1:1137047/141‑1 (MQ=255)
         tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGttt                                                                                                                                    >  1:375198/1‑141 (MQ=255)
            aaTAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATACTCATCACGAAAGCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTAc                                                                                                                                 <  1:1123488/141‑1 (MQ=255)
            aaTAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAATCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTAc                                                                                                                                 <  2:528805/141‑1 (MQ=255)
               aGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAgg                                                                                                                              >  1:972764/1‑141 (MQ=255)
                 tttAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAGTACATGGTTTTACAGGCa                                                                                                                            <  1:1038881/141‑1 (MQ=255)
                  ctAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTATTACATGGTTTTACAGGCaa                                                                                                                           <  1:716719/140‑1 (MQ=255)
                   tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTGTCTTTTTTGAGGAAGGTAAACGTGACGTGTTATTACTAAATGGTTTTACAGGAAAt                                                                                                                          >  2:75741/1‑141 (MQ=255)
                     aCAAGAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGATCTTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTc                                                                                                                        <  1:454236/141‑1 (MQ=255)
                      aaaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCg                                                                                                                       <  2:440842/140‑1 (MQ=255)
                       aaTTAGGTATGTAAAATATATAGGCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTAGGCAGATAAAATTACCAAACCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGt                                                                                                                      <  2:186934/141‑1 (MQ=255)
                       aaTAAGCTATGTCACATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATCAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGt                                                                                                                      <  2:863971/141‑1 (MQ=255)
                             cTATGTAAAATATATAGCCTAGTTAAGTAGGCAAAGGGAGCGTTAGATTTATGCAGTCACCTCAACCGAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATg                                                                                                                <  1:568230/141‑1 (MQ=255)
                              tATGTAAAATATACAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGt                                                                                                               <  2:863034/141‑1 (MQ=255)
                              tATCTAAAATATATAGCCAAGTTAAGTGTGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGtacgcact                                                                                                               <  2:602463/141‑8 (MQ=255)
                               aTGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTGTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGGTTTACAGGCAATTCGTCTGATGtt                                                                                                              <  2:1001126/141‑1 (MQ=255)
                                    aaaTATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATTCAGTTAAAATCACCAAAACCTTTCTTTTTTGAGGAAAGTAAACCTGCGGTGTTATTAGTACATGGTTTCACATGCAACTGGTCTGATGTGCGTCa                                                                                                         >  2:788917/1‑141 (MQ=255)
                                    aaaTATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCa                                                                                                         <  2:278571/141‑1 (MQ=255)
                                           taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                  <  2:252593/141‑1 (MQ=255)
                                           taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                                                  <  2:146169/141‑1 (MQ=255)
                                             gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                                                <  2:747503/141‑1 (MQ=255)
                                              ccTAGTTAAGTATGCAAAGGGAGCGGTAGATTTAGGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTAGTCTGATGTTCGTCAATTAGGTCGa                                                                                               >  1:206727/1‑141 (MQ=255)
                                                tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                                             <  1:445006/141‑1 (MQ=255)
                                                 aGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTGTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCGATTCGTCTGAATTTCGTCAATTAGGATGAttt                                                                                            >  1:798839/1‑141 (MQ=255)
                                                  gTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTAAAAGTGACGTGTTGTTACTAGATGGTTTTATAGGCAATTAGGCTGGTGTTTGTTAATTTGGTCGTtttt                                                                                           >  2:992248/1‑141 (MQ=255)
                                                   ttAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGAAGTTCGGCAATTAGGTCgcatttc                                                                                          >  2:586902/1‑135 (MQ=255)
                                                    tAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACATTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATAAGGTCGATTTTTa                                                                                         >  1:271929/1‑141 (MQ=255)
                                                     aaGGATGCAAAGCGGGAGTTAGATTTATGCAGATAAAATTACCAAATAACTTTTTGTGTGAGGAAGTTAAACGTGCCGTGTTATGACTACATGGTTTTACAGGTAATTCGTCTGATGTTCGTCAAGTAGGTCGTTTTTTAc                                                                                        <  2:361230/141‑1 (MQ=255)
                                                      aGTATTCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       <  1:627025/141‑1 (MQ=255)
                                                      aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       >  1:950114/1‑141 (MQ=255)
                                                      aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       >  1:65949/1‑141 (MQ=255)
                                                      aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                                       >  1:1112956/1‑141 (MQ=255)
                                                       gTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTGTTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGGCGATTTTTACaa                                                                                      >  1:35593/1‑141 (MQ=255)
                                                       gTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaa                                                                                      >  2:1180271/1‑141 (MQ=255)
                                                        tATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaaa                                                                                     >  1:1044768/1‑141 (MQ=255)
                                                        tATGCAAAGGGAGAGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaaa                                                                                     <  1:25833/141‑1 (MQ=255)
                                                           gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                                                  <  2:375198/141‑1 (MQ=255)
                                                             aaaGGGAGCGTTAGATTTATGCAGATAAAATTACCAAATCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaagaaa                                                                                <  2:173246/141‑1 (MQ=255)
                                                              aaGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaagaaag                                                                               >  1:113988/1‑141 (MQ=255)
                                                               aGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAgg                                                                              <  2:873997/141‑1 (MQ=255)
                                                                  gAGCGTTAGATTTATGCAGATAAAATTACCAAACCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTta                                                                           <  1:1111899/141‑1 (MQ=255)
                                                                    gCGTTAGATTTATGCATATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                         <  2:933015/141‑1 (MQ=255)
                                                                    gCGTTAGATTTATGCAGATAGAATTACCAAAACCTTTCTTTTTTCAGGAAGGAAAACGCGCCGTGTTATTAGTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGTTCGATTTTTACAAAAGAAAGGTtata                                                                         >  1:729660/1‑141 (MQ=255)
                                                                    gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAGTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                                         >  1:836618/1‑141 (MQ=255)
                                                                      gTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTTGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATAca                                                                       >  1:947707/1‑141 (MQ=255)
                                                                       ttAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTTAACGTGCCGGGTTATTACTACATGGTTTTACAGGCAATTCGTTTGATATTCGTCGATTAGGTCGATTTTTACAAAAGAAAGATtaaaaat                                                                      >  2:245787/1‑136 (MQ=255)
                                                                            tttATGCAGATAAAATTACCAAATCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCatat                                                                 <  2:852326/141‑1 (MQ=255)
                                                                             ttATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTATAAAAGAAAGGTTATACATCATATg                                                                <  1:765048/141‑1 (MQ=255)
                                                                                  cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAGTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCg                                                           >  1:709525/1‑141 (MQ=255)
                                                                                   aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                          <  1:178287/141‑1 (MQ=255)
                                                                                   aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                          <  1:743413/141‑1 (MQ=255)
                                                                                   aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGCGTTATTACTACATGGGTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                          <  1:273607/141‑1 (MQ=255)
                                                                                    gATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAACCGTACCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCa                                                         <  2:957046/141‑1 (MQ=255)
                                                                                         aaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATg                                                    >  1:217460/1‑141 (MQ=255)
                                                                                         aaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATg                                                    >  1:416897/1‑141 (MQ=255)
                                                                                            tACCAAAACCTTTCTTTTTTTAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATACACCGCAATATGAAg                                                 >  1:748966/1‑141 (MQ=255)
                                                                                            tACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAg                                                 >  1:830751/1‑141 (MQ=255)
                                                                                             aCCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAgg                                                <  2:790159/141‑1 (MQ=255)
                                                                                               cacaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGTCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              <  2:800757/138‑1 (MQ=255)
                                                                                               cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              >  1:1018656/1‑141 (MQ=255)
                                                                                               cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              <  2:1044768/141‑1 (MQ=255)
                                                                                               cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              >  1:912066/1‑141 (MQ=255)
                                                                                               cAAAACCTTGCTTTTTTTAGGAAGGTAAACGTGCTGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                              <  2:206727/141‑1 (MQ=255)
                                                                                                 aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                            >  1:582683/1‑141 (MQ=255)
                                                                                                  aaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGACATATTTCTTATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                                           <  1:871187/141‑1 (MQ=255)
                                                                                                   atccTTTCTTTTGTGAGGAAGGTAAAGGTGCCGTGTTATTATGACAGTGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCAGCTGCACCGCAATATGAAGGCCAcg                                           <  2:416050/139‑1 (MQ=255)
                                                                                                   agctttttttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                                           <  2:710896/137‑1 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGGGGAAGGTAAACGTGCCGTGTTATTACCACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:731365/141‑1 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAGAACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:927734/141‑1 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:920584/141‑1 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          >  1:481432/1‑141 (MQ=255)
                                                                                                    aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                          <  1:731339/141‑1 (MQ=255)
                                                                                                       tttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGCAAGGTTATGCATCGTATGCAGCGCAATGTGTAGGCCACGAGGc                                       >  2:936208/1‑141 (MQ=255)
                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                     >  1:945295/1‑141 (MQ=255)
                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                     >  2:1174458/1‑141 (MQ=255)
                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCAACGCAATATGAAGGCCACGCGGcac                                     >  2:770807/1‑141 (MQ=255)
                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGACTCATATACATAGAAGGTTCAAGATCATATACACCGCAATATGAAGGCCACGCGGcac                                     >  1:562491/1‑141 (MQ=255)
                                                                                                           ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca                                   >  1:785324/1‑141 (MQ=255)
                                                                                                           ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca                                   >  2:877390/1‑141 (MQ=255)
                                                                                                                tGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGa                              <  1:258177/141‑1 (MQ=255)
                                                                                                                 ggcgtgggTAAACGTGCCGTGTTATTACTACTTGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGACACGCGGCACCACCAGAt                             <  1:236313/135‑1 (MQ=255)
                                                                                                                    gAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTTGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGTAATATGAAGGCCAGGCGGCACCAACAGATGaa                          >  2:20918/1‑141 (MQ=255)
                                                                                                                     aaGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaaa                         <  1:988960/141‑1 (MQ=255)
                                                                                                                         tAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCCCCAGATGAAATACt                     >  2:1137212/1‑141 (MQ=255)
                                                                                                                           aaCGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCCTATGCACCGCAATATTAAGGCCACGCGGCACCACCAGATGAAATACTGa                   <  2:901165/141‑1 (MQ=255)
                                                                                                                             cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                 >  2:119607/1‑141 (MQ=255)
                                                                                                                             cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGACCACGCGGCAACACAAGATGAAAAACTGaaa                 >  1:911753/1‑141 (MQ=255)
                                                                                                                               tGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATCCATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATc               <  1:717166/141‑1 (MQ=255)
                                                                                                                                   tggtTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCAACGCGTCACCACCAGATGAAATACTGAAATCTAGt           <  1:284564/139‑1 (MQ=255)
                                                                                                                                        tttaCTACATGGTTTTACAGGCAATTCGTGTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCttt      <  2:1154181/140‑1 (MQ=255)
                                                                                                                                            ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt  >  2:252673/1‑141 (MQ=255)
                                                                                                                                            ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATCAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt  <  2:881562/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTT  >  CP000730/864606‑864885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: