Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,146,228 A→G 3.6% E92G (GAA→GGA)  pheS → phenylalanine‑‑tRNA ligase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,146,2280AG 3.6% 359.8 / 2.4 116E92G (GAA→GGA) pheSphenylalanine‑‑tRNA ligase alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (58/52);  new base G (3/2);  total (62/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 123 total aligned reads displayed.

GAAATTGATGAAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAACAA  >  CP000730/1146088‑1146367
                                                                                                                                            |                                                                                                                                            
gAAATTGATGAAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTga                                                                                                                                              >  1:1034722/1‑141 (MQ=255)
 aaaTTGATGAAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTgaa                                                                                                                                             <  1:441691/141‑1 (MQ=255)
   aTTGATGAAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTgaaga                                                                                                                                           <  1:825413/141‑1 (MQ=255)
    ttGATGAATGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAACAATTGGCTgaagaa                                                                                                                                          <  1:888227/141‑1 (MQ=255)
      gatgaAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAAc                                                                                                                                        <  2:1149569/141‑1 (MQ=255)
       atgaAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGGAGAAACa                                                                                                                                       >  1:147978/1‑141 (MQ=255)
       atgaAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGGAGAAACa                                                                                                                                       >  1:752707/1‑141 (MQ=255)
        tgcaGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAAGTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACaa                                                                                                                                      <  1:501945/138‑1 (MQ=255)
        tgaAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAACCAAGTCAAAATGAATTAGATGAAAGACAACAGATGTTAATTAAAGAAAAATTAAATAAGCAATGGGCTGAAGgaagaa                                                                                                                                      >  2:275080/1‑141 (MQ=255)
        tgaAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAACTAAGCAATTGGCTGAAGAAACaa                                                                                                                                      >  1:268364/1‑141 (MQ=255)
        tgaAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACaa                                                                                                                                      >  1:293679/1‑141 (MQ=255)
        tgaAGGATTTGCCCAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGCCAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACaa                                                                                                                                      <  1:85823/141‑1 (MQ=255)
          aaGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAGAAAGTGAATGAATTGCGGCAAACAATTCACACTGACTTAGGTGAAAGACAACAGATGTGAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACGAtt                                                                                                                                    >  2:961351/1‑141 (MQ=255)
            ggATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGa                                                                                                                                  <  1:690356/141‑1 (MQ=255)
            ggATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGa                                                                                                                                  >  1:182121/1‑141 (MQ=255)
             gATTTGCCCAAAGAAGATAAACCTGCCTTAGGTCAAAAAGAGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGAt                                                                                                                                 <  2:962869/141‑1 (MQ=255)
              aTTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATg                                                                                                                                >  1:371412/1‑141 (MQ=255)
                ttGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTa                                                                                                                              >  1:259189/1‑141 (MQ=255)
                ttGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTa                                                                                                                              >  1:8123/1‑141 (MQ=255)
                ttGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTa                                                                                                                              >  1:473400/1‑141 (MQ=255)
                   ccGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGATGAAGAAACAATTGATGTATCa                                                                                                                           >  2:1201053/1‑141 (MQ=255)
                    cGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCAt                                                                                                                          >  1:1095410/1‑141 (MQ=255)
                      aaTGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTa                                                                                                                        <  1:164055/141‑1 (MQ=255)
                       aTGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTAc                                                                                                                       <  2:1029331/141‑1 (MQ=255)
                        tGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTAcc                                                                                                                      <  2:817494/141‑1 (MQ=255)
                              tAAACCTGCGTTTGCTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATAGGCTGAAGAAACAATTGATGTATCATTACCAGgtcg                                                                                                               >  1:662602/1‑141 (MQ=255)
                                aaCCTGCGTTTGGTCAAAAAGTGAATGAATTCCGTCTAACAATTAAAATTAAATTATATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGCTGTATCATTACCAGgtcgtc                                                                                                             >  1:169489/1‑141 (MQ=255)
                                    tGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCatat                                                                                                         >  1:1101687/1‑141 (MQ=255)
                                      cGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGGTGAAGAAACAATTGATGTATCATTACAAGgtaggcagatgg                                                                                                        >  2:325500/1‑131 (MQ=255)
                                        tttGTTCAAAAAGTGAATTAAATGCCTCAAAAAATACAAAATTAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATGAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGaa                                                                                                     <  1:246436/141‑1 (MQ=255)
                                          tGGTCAAAAAGTGATTGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAAt                                                                                                   <  1:1000359/141‑1 (MQ=255)
                                          tGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAAt                                                                                                   <  2:684117/141‑1 (MQ=255)
                                          tGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAAt                                                                                                   <  2:1010781/141‑1 (MQ=255)
                                          tGGGCAAAAAGTGAATGAAGTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAAt                                                                                                   <  1:370303/141‑1 (MQ=255)
                                              cAAAAAGTGAATGAATTGCGTCAAACAATTTAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGt                                                                                               <  1:822962/141‑1 (MQ=255)
                                               aaaaaGGGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGtt                                                                                              <  2:871519/141‑1 (MQ=255)
                                                aaaaGTGAATGAAATGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTc                                                                                             <  1:522906/141‑1 (MQ=255)
                                                 aaaGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCa                                                                                            >  1:160422/1‑141 (MQ=255)
                                                  aaGTGAATGAATTGCGTTAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTTGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCaa                                                                                           <  2:296821/141‑1 (MQ=255)
                                                  aaGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCaa                                                                                           <  1:1028262/141‑1 (MQ=255)
                                                  aaGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGACGTCATATTGAAATCGGTTCaa                                                                                           >  2:1166297/1‑141 (MQ=255)
                                                      gactgcctctcGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCa                                                                                       <  2:536131/131‑1 (MQ=255)
                                                              tGCGTAAAACAATTCAAAATGATTTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTaa                                                                               <  1:424739/141‑1 (MQ=255)
                                                              tGCGACAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTGAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTaa                                                                               <  1:6112/141‑1 (MQ=255)
                                                               gCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAac                                                                              <  2:697464/141‑1 (MQ=255)
                                                                cGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTGAGTTAAAGAAAAATTAACTAAGCAATTGGCTGAAGAAACAATCGATGTATCATTACCAGGTCGTCATACTGAAATCGGTTCAAAGCATCCATTAaca                                                                             <  1:773018/141‑1 (MQ=255)
                                                                 gtaaaaCAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAacac                                                                            <  1:836909/138‑1 (MQ=255)
                                                                  tCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGATCAAAGCATCCATTAACACg                                                                           >  1:806546/1‑141 (MQ=255)
                                                                        aaTTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAAt                                                                     >  2:942152/1‑141 (MQ=255)
                                                                        aaTTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACAAGTACAAt                                                                     >  2:114971/1‑141 (MQ=255)
                                                                         aTTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATa                                                                    >  1:1003135/1‑141 (MQ=255)
                                                                         aTTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATa                                                                    >  1:685000/1‑141 (MQ=255)
                                                                          ttCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATag                                                                   >  1:968956/1‑141 (MQ=255)
                                                                            cAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATagaa                                                                 <  1:83453/141‑1 (MQ=255)
                                                                                    aTTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGa                                                         <  1:1158628/141‑1 (MQ=255)
                                                                                       aGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGa                                                      >  2:54047/1‑141 (MQ=255)
                                                                                        aaTGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGAc                                                     <  2:1005203/140‑1 (MQ=255)
                                                                                             aaaacaaaaGATGTTAGTTAAAGAAAAATTAAATAAGCAATTTGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTAtt                                                <  2:1066788/138‑1 (MQ=255)
                                                                                              aGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTc                                               >  1:943940/1‑141 (MQ=255)
                                                                                               atcaagtgATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCt                                              <  2:293679/134‑1 (MQ=255)
                                                                                                 caacaGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCtta                                            >  2:683423/1‑141 (MQ=255)
                                                                                                 caacaGATGTTAGCTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTTtta                                            >  2:286412/1‑141 (MQ=255)
                                                                                                  aacaGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGCCGAAATTGAAGAGTTATTCttag                                           <  2:1003135/141‑1 (MQ=255)
                                                                                                  aacaGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCttag                                           >  2:278535/1‑141 (MQ=255)
                                                                                                  aacaGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGCATCATTACCTGGTCGTCATATTGAAACCTGCGCCAATCATCCATTAACATGTACAATAGAAGAAATTGAAGACTTATTCttag                                           >  2:104356/1‑141 (MQ=255)
                                                                                                    caGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCttaggt                                         >  1:187772/1‑141 (MQ=255)
                                                                                                     aGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATCCttaggtt                                        >  2:502770/1‑141 (MQ=255)
                                                                                                      gATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTACCACGTACAATAGAAGAAAATGAAGAATTATTCttaggttt                                       >  2:516707/1‑141 (MQ=255)
                                                                                                       aTGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTCCAATAGAAGAAATTGAAAACCTATTCttaggttta                                      >  2:345248/1‑141 (MQ=255)
                                                                                                        tGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCttaggtttag                                     >  1:456708/1‑141 (MQ=255)
                                                                                                           tagttaAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTtaggtttaggtt                                  >  2:480480/1‑141 (MQ=255)
                                                                                                                 aaGAAAAATTAAATAAGCAATTGGCTGGAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGaaa                            <  2:147978/141‑1 (MQ=255)
                                                                                                                 aaGAAAAATTAAATAAGCAATTGGCTGGAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGaaa                            <  2:752707/141‑1 (MQ=255)
                                                                                                                   gAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAAtt                          >  1:945629/1‑141 (MQ=255)
                                                                                                                   gAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAAtt                          >  1:531687/1‑141 (MQ=255)
                                                                                                                    aaaaaTTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATtg                         <  1:1201053/141‑1 (MQ=255)
                                                                                                                    aaaaaTTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATtg                         >  1:268314/1‑141 (MQ=255)
                                                                                                                    aaaaaTTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATtg                         >  2:115640/1‑141 (MQ=255)
                                                                                                                      aaaTTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATtgtg                       >  1:786984/1‑141 (MQ=255)
                                                                                                                      aaaTTAAATAAGCAATTGGCTGAAGAAACAACTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATtgtg                       <  2:632987/141‑1 (MQ=255)
                                                                                                                           aaaaaaGCAATTTGCTGAAGAAAAAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATgg                  <  1:1075147/141‑1 (MQ=255)
                                                                                                                           aaaTAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCTTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATgg                  >  1:337857/1‑141 (MQ=255)
                                                                                                                           aaaTAAGCAATTGGCGGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATgg                  >  2:108729/1‑141 (MQ=255)
                                                                                                                             aTAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGat                >  1:356344/1‑141 (MQ=255)
                                                                                                                              tAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGata               >  1:1102305/1‑141 (MQ=255)
                                                                                                                              tAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGata               >  1:990720/1‑141 (MQ=255)
                                                                                                                              tAAGCAATTGGCTGAAGAAACAATTGATGTATCAATACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGata               <  2:461284/141‑1 (MQ=255)
                                                                                                                               aaGCATGTGGCTGAAGAAACAATTGATGTATCCTTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATATAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAACTGTGAATGGatat              <  1:800746/141‑1 (MQ=255)
                                                                                                                                aGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATg             >  1:851384/1‑141 (MQ=255)
                                                                                                                                aGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTCTAGGTTCTGAAATTGTGAATGGATATg             >  2:57444/1‑141 (MQ=255)
                                                                                                                                  cAATTGGCTTAAGAAACAATTGATCTATCATTCAATGTGGGTCATATTGAAATACGATCAAAACATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAAATGTGAATGTATATGaa           >  1:876288/1‑141 (MQ=255)
                                                                                                                                  aaaTTGGCTGAAGAAACAAGTAATGTACCATTACCAGGTCTTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTAAAATAGTAGGACTAGTATATTTATTCTGAGGTTTAGGTTATGAAATTGTGAATGGATATGaa           <  2:961650/140‑1 (MQ=255)
                                                                                                                                    aTTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGt         >  1:358/1‑141 (MQ=255)
                                                                                                                                    aTTGGCTGAAGAAACAATTGATGTAGCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTTAGTTAACTAATTGTGAATGGATATGAAGt         <  2:255963/141‑1 (MQ=255)
                                                                                                                                       ggCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGa      >  1:301187/1‑141 (MQ=255)
                                                                                                                                       ggCTGAAGAAACAATTGACGTATCATTACCAGGTCGTCATATTGAAATCGGTTGAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGa      <  1:610098/141‑1 (MQ=255)
                                                                                                                                         cTGAAGAAACCACTAATGTAATATACCCAGGTCTTAATATTGAAATCGGTTCAAAGCATCAATTAACACGTACAATAGAATAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAAc    <  1:493239/141‑1 (MQ=255)
                                                                                                                                         cTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAAc    <  2:1101687/141‑1 (MQ=255)
                                                                                                                                           gaagaaACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAACaa  <  2:473400/141‑1 (MQ=255)
                                                                                                                                           gaagaaACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAACTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAACaa  >  2:786937/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GAAATTGATGAAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGTCAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATGTTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCAGGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAAGACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAACAA  >  CP000730/1146088‑1146367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: