BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,174,980	T→C	4.3%	A36A (GCA→GCG)	USA300HOU_1105 ←	exotoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,174,980	0	T	C	4.3%	292.2 / 2.9	94	A36A (GCA→GCG)	USA300HOU_1105	exotoxin
Reads supporting (aligned to +/- strand): ref base T (44/45); new base C (2/2); total (46/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

ACCCACGTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAATTACCCAAAGT  >  CP000730/1174840‑1175120

aCCCACGTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCtttt                                                                                                                                              >  1:1193371/1‑141 (MQ=255)

 cccACGTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCCCTGATATTATAGGCTTTTg                                                                                                                                             >  2:275825/1‑141 (MQ=255)

  ccACGTTTGCAATGTTGCATGAATGCTACCATTGGGCGTTTGACATAACAATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGc                                                                                                                                            <  2:895145/141‑1 (MQ=255)

    ccGTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCtt                                                                                                                                          <  2:860893/140‑1 (MQ=255)

     cGTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTc                                                                                                                                         >  1:1072256/1‑141 (MQ=255)

      gTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCCTTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCa                                                                                                                                        <  1:935692/141‑1 (MQ=255)

      gTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCa                                                                                                                                        >  2:471196/1‑141 (MQ=255)

      gTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCa                                                                                                                                        >  1:1026330/1‑141 (MQ=255)

      gTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTCGCTTCa                                                                                                                                        >  2:1023623/1‑141 (MQ=255)

      gTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTCGCTTCa                                                                                                                                        >  2:113181/1‑141 (MQ=255)

        ttGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGa                                                                                                                                      >  2:466181/1‑141 (MQ=255)

           cAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGa                                                                                                                                   <  1:111537/141‑1 (MQ=255)

              tGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGaaaa                                                                                                                                >  2:295862/1‑141 (MQ=255)

              tGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGaaaa                                                                                                                                >  2:1089929/1‑141 (MQ=255)

              tGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGaaaa                                                                                                                                >  1:176253/1‑141 (MQ=255)

                   gcattcatgcaacATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAATTGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGa                                                                                                                           >  2:961553/13‑141 (MQ=255)

                    aTGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGaa                                                                                                                          >  2:515069/1‑140 (MQ=255)

                    aTGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGAt                                                                                                                          >  2:871694/1‑141 (MQ=255)

                    aTGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGAt                                                                                                                          >  2:1068320/1‑141 (MQ=255)

                    aTGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGAt                                                                                                                          >  2:114447/1‑141 (MQ=255)

                       aaTGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTgg                                                                                                                       <  2:1200095/141‑1 (MQ=255)

                       aaTGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTgg                                                                                                                       >  1:3324/1‑141 (MQ=255)

                                aTTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCCCTGATATTATAGGCTTTTGCTTCAGATGAAAACCTGATTGGTGTTTTAgg                                                                                                              <  1:165105/141‑1 (MQ=255)

                                 ttGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGa                                                                                                             <  2:939353/141‑1 (MQ=255)

                                    ggCCTTTGCCATGACCAGTTTCCAGATAATGAAAAATGCGGCTGGGTGTAATATGTTATGAGTGCCGTGACATACGCCGCGTTCTCCCTGATATTATAGGCTTTTGCTCCAGATGAAAAACTGCTTGGTGTTTTAGGAAGt                                                                                                          <  2:298625/141‑1 (MQ=255)

                                          tGCCATAACCATTTTCCAGATAATGAAAAAGGCGGCTGAGTGTAATATTTGATTAGTTCATGGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGtt                                                                                                    >  1:348362/1‑141 (MQ=255)

                                            ccATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGa                                                                                                  <  2:1092339/141‑1 (MQ=255)

                                             cATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTACTTCATTGATATTAGTCTCGTTTTCACTGATATGATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGCGTTGAt                                                                                                 <  2:753053/141‑1 (MQ=255)

                                             aaTAACCATTTGCCAAGTAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCATTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGAt                                                                                                 <  2:923092/140‑1 (MQ=255)

                                               tAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGGTTGGTGTTTTAGGAAGTTGTGTGGATGt                                                                                               >  1:30819/1‑141 (MQ=255)

                                                 aCCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAg                                                                                             <  1:379031/141‑1 (MQ=255)

                                                   cATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGtt                                                                                           <  1:720598/141‑1 (MQ=255)

                                                   cATATTCCAGATAAAGAAAAATGCGGCTGAGTGTAATATTTGATTAGTGCAATGATATTAGTCGCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGtt                                                                                           <  1:887372/141‑1 (MQ=255)

                                                     ttttCCAGATAATGAAAAAAGCGGCTGAGTGTAATGTGTGATTAGTGGAGTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTc                                                                                         >  1:1138029/1‑141 (MQ=255)

                                                       ttCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTCGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGAGGTGATGTAGttttac                                                                                       >  2:1198153/1‑138 (MQ=255)

                                                       ttCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTa                                                                                       >  1:239840/1‑141 (MQ=255)

                                                           aGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATCAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAg                                                                                   >  2:879153/1‑141 (MQ=255)

                                                            gATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGt                                                                                  <  1:757059/141‑1 (MQ=255)

                                                              tAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTGATTGATATTAGTCTCGTTTTCACGGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTaa                                                                                <  2:680119/141‑1 (MQ=255)

                                                              tAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTaa                                                                                >  1:398088/1‑141 (MQ=255)

                                                               aaTCAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGCTATTCGTGTCGATATGAGTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGTTTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAGAAGTAAc                                                                               <  2:843124/141‑1 (MQ=255)

                                                                    aaaaTGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGc                                                                          >  1:748408/1‑141 (MQ=255)

                                                                      aaTGCTGCCGAGGGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTg                                                                        <  1:1110883/141‑1 (MQ=255)

                                                                      aaTGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTg                                                                        <  1:471196/141‑1 (MQ=255)

                                                                      aaTGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTg                                                                        <  1:980725/141‑1 (MQ=255)

                                                                      aaTGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTg                                                                        <  1:672868/141‑1 (MQ=255)

                                                                      aaTGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTg                                                                        <  1:183421/141‑1 (MQ=255)

                                                                      aaTGCGGCGGGGTGTAATAGTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTg                                                                        <  2:42456/141‑1 (MQ=255)

                                                                       aTGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGt                                                                       <  1:314714/141‑1 (MQ=255)

                                                                          cGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGa                                                                    <  2:459494/141‑1 (MQ=255)

                                                                            gcccaGTGTAACATCCGATTACTACAATGATAATAATCACGACTACACCGATATTATAGGCTAAAGCATAAAAAAAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATa                                                                  <  2:622706/137‑1 (MQ=255)

                                                                             cTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAAAAATGCTGTTGATaa                                                                 >  2:133881/1‑141 (MQ=255)

                                                                             cTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTGTTGCGTCAGATGAAAAACTGATTGAAGTTTTAGGAAGTTGTGTTGATGTAGTGTCTAAAAGTAACAATGCTGTTGATaa                                                                 <  1:464500/141‑1 (MQ=255)

                                                                                  tttAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTa                                                            <  1:860585/139‑1 (MQ=255)

                                                                                   gtAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTaa                                                           >  2:67154/1‑141 (MQ=255)

                                                                                   gtAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTaa                                                           >  2:11381/1‑141 (MQ=255)

                                                                                       tatTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTa                                                       >  2:571655/1‑141 (MQ=255)

                                                                                       tatTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTa                                                       <  1:295862/141‑1 (MQ=255)

                                                                                       tatTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTa                                                       <  1:1089929/141‑1 (MQ=255)

                                                                                              ttAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTCGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCatga                                                <  1:1023623/141‑1 (MQ=255)

                                                                                              ttAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTCGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCatga                                                <  1:113181/141‑1 (MQ=255)

                                                                                               tAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCatgat                                               <  1:205770/141‑1 (MQ=255)

                                                                                               tAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCatgat                                               >  1:608621/1‑141 (MQ=255)

                                                                                                aGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCatgatg                                              <  1:961553/141‑1 (MQ=255)

                                                                                                 gTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGt                                             <  1:61814/141‑1 (MQ=255)

                                                                                                 gTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGt                                             <  1:908768/141‑1 (MQ=255)

                                                                                                  ttCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTGATTCATGATGtt                                            >  1:168919/1‑141 (MQ=255)

                                                                                                   tCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTc                                           <  2:444429/141‑1 (MQ=255)

                                                                                                    taTTGAGATTAGTCCTGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCCTGATGTTCt                                          <  2:880875/140‑1 (MQ=255)

                                                                                                      ttGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCttt                                        >  2:434231/1‑141 (MQ=255)

                                                                                                        gATATTAGTCTCGGTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGAAATATTAGCAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAACGCTGTTGATAAAACTAATTTATTCATGATGTTCttttt                                      <  1:275923/141‑1 (MQ=255)

                                                                                                        gATATTAGTCGCGTTTTCACTGATATTATAGGCTTTTGCTGCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACCAATGTATTCATGATGTTCttttt                                      <  2:548396/141‑1 (MQ=255)

                                                                                                         atatTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTAATGATGTTCTTTTTc                                     >  1:732649/1‑141 (MQ=255)

                                                                                                         agatTAGTCTCGTTTTCGTATATATTTTAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTc                                     <  2:508973/139‑1 (MQ=255)

                                                                                                          tatTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCa                                    >  1:115576/1‑141 (MQ=255)

                                                                                                              tggctcGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATg                                <  1:718952/138‑1 (MQ=255)

                                                                                                              aGTCTCGTTTTGACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTTGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATg                                <  1:497264/141‑1 (MQ=255)

                                                                                                              aGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATg                                >  1:1169007/1‑141 (MQ=255)

                                                                                                                 ctcGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGaaa                             >  2:398213/1‑141 (MQ=255)

                                                                                                                 ctcGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGaaa                             >  1:477521/1‑141 (MQ=255)

                                                                                                                         cccTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCtt                     <  2:528584/139‑1 (MQ=255)

                                                                                                                         cACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTGAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCtt                     >  2:574060/1‑141 (MQ=255)

                                                                                                                           cTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAACTCTCCtttt                   >  2:411541/1‑140 (MQ=255)

                                                                                                                           cTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTg                   >  1:351727/1‑141 (MQ=255)

                                                                                                                              atatTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAGATCTCCTTTGCGt                >  2:859852/1‑141 (MQ=255)

                                                                                                                              atatTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCgg                >  1:1041975/1‑140 (MQ=255)

                                                                                                                               ttttatAGGCTTTTGCTTCAGATGAAAAACTGATTGTTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTATCATTGTTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTTATACGAAAATCTCCTTTGCGTg               >  2:1095333/3‑141 (MQ=255)

                                                                                                                                   ataGGCTTTTGCTTCAGATGAAAACCTGATTGGTGTTTTAGGCAGCTGCGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAAATATTTTTTGCAGGAAGGTCTTTTTCATATGAAAATCTCCTCTCCGTGAAtt           >  2:419827/1‑141 (MQ=255)

                                                                                                                                   ataGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAAtt           >  2:1125247/1‑141 (MQ=255)

                                                                                                                                   ataGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAAtt           <  2:42518/141‑1 (MQ=255)

                                                                                                                                     aGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAATTAc         >  1:505717/1‑141 (MQ=255)

                                                                                                                                     aGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAATTAc         <  2:239840/141‑1 (MQ=255)

                                                                                                                                      ggCTTTTGCTTCCGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAATTAcc        <  2:114661/141‑1 (MQ=255)

                                                                                                                                      ggCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGCATTAcc        <  2:660196/141‑1 (MQ=255)

                                                                                                                                       gCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATGCATGGTGGTCTTTTTCATATGAAAATCTCCTTTGCGTGAATTcccc       >  2:540793/1‑141 (MQ=255)

                                                                                                                                            tGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAATTACCCAAAGt  >  2:697403/1‑141 (MQ=255)

                                                                                                                                            tGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAATTACCCAAAGt  >  2:565744/1‑141 (MQ=255)

ACCCACGTTTGCAATGTTGCATGAATGCTACCATTGGGCTTTTGCCATAACCATTTTCCAGATAATGAAAAATGCGGCTGAGTGTAATATTTGATTAGTTCATTGATATTAGTCTCGTTTTCACTGATATTATAGGCTTTTGCTTCAGATGAAAAACTGATTGGTGTTTTAGGAAGTTGTGTTGATGTAGTTTCTAAAAGTAACAATGCTGTTGATAAAACTAATTTATTCATGATGTTCTTTTTCATATGAAAATCTCCTTTGCGTGAATTACCCAAAGT  >  CP000730/1174840‑1175120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`