Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,266,747 C→A 4.8% P13T (CCT→ACT)  topA → DNA topoisomerase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,266,7470CA 4.8% 291.4 / 4.2 95P13T (CCT→ACT) topADNA topoisomerase A
Reads supporting (aligned to +/- strand):  ref base C (42/48);  new base A (2/3);  total (44/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

TAAAAAATAGACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGAACCAAAATATATAACAAT  >  CP000730/1266607‑1266886
                                                                                                                                            |                                                                                                                                           
taAAAAATAGACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGc                                                                                                                                             >  2:454712/1‑141 (MQ=255)
taAAAAATAGACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGc                                                                                                                                             >  2:219476/1‑141 (MQ=255)
taAAAAATAGACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGc                                                                                                                                             >  1:744300/1‑141 (MQ=255)
aaaaaaaTAGACAGGGAAAAGGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGc                                                                                                                                             <  2:7869/140‑1 (MQ=255)
     aaTAGACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTgca                                                                                                                                        <  1:1045298/141‑1 (MQ=255)
     aaTAGACAGGGAAAATGATTTGTTTAGATATAAAAAGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTTGCAGATAATGTAGTCATTGTTGAATCGCCTgca                                                                                                                                        >  2:343352/1‑141 (MQ=255)
      aTAGACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGTGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTgcaa                                                                                                                                       <  1:629168/141‑1 (MQ=255)
          aCAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTgcaaaagc                                                                                                                                   >  1:537666/1‑141 (MQ=255)
               gAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCagaaa                                                                                                                              >  1:152959/1‑141 (MQ=255)
               gAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCaaaaa                                                                                                                              >  1:740669/1‑141 (MQ=255)
               gAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTAGCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCaaaaa                                                                                                                              <  2:1084815/141‑1 (MQ=255)
                 aaaTGAGTGGTTGAGATATAAGACGTTGACAAAAGCAAAATTAAGGGTTGATCATTTAGCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTTGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAAcc                                                                                                                            <  1:835609/141‑1 (MQ=255)
                   aTGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCAt                                                                                                                          >  2:930456/1‑141 (MQ=255)
                   aTGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCAt                                                                                                                          >  2:151063/1‑141 (MQ=255)
                    tGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCAtt                                                                                                                         <  1:963688/141‑1 (MQ=255)
                    tGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCAtt                                                                                                                         <  1:1106110/141‑1 (MQ=255)
                        ttGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGaaa                                                                                                                     >  1:670469/1‑141 (MQ=255)
                        ttGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGaaa                                                                                                                     >  1:1139863/1‑141 (MQ=255)
                        ttGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGaaa                                                                                                                     <  1:546005/141‑1 (MQ=255)
                           tttAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGt                                                                                                                  >  2:434368/1‑141 (MQ=255)
                              atatatAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTAtt                                                                                                               <  1:440694/139‑1 (MQ=255)
                               gATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTAttt                                                                                                              <  1:174981/141‑1 (MQ=255)
                               aatatAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTAttt                                                                                                              <  1:858752/140‑1 (MQ=255)
                                 aaaaaaaAGATGACAAAAGAAAAATTAAGCGGTTATAATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCAAAAAAAAAAAAAAACCATTGAAAAGTATTTAg                                                                                                            <  2:429782/138‑1 (MQ=255)
                                   taAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGt                                                                                                          >  2:266172/1‑141 (MQ=255)
                                    aaaaCGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTa                                                                                                         <  2:1178083/141‑1 (MQ=255)
                                    aaaaCGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTa                                                                                                         <  2:891372/141‑1 (MQ=255)
                                      aaCGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAAGTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAg                                                                                                       <  1:160090/141‑1 (MQ=255)
                                       aCGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGa                                                                                                      <  1:1078862/141‑1 (MQ=255)
                                       aCGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGa                                                                                                      >  1:739596/1‑141 (MQ=255)
                                        cGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGaa                                                                                                     <  1:415418/141‑1 (MQ=255)
                                        cGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGaa                                                                                                     <  1:1014965/141‑1 (MQ=255)
                                        agTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGaa                                                                                                     <  1:1081918/140‑1 (MQ=255)
                                            gACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAatat                                                                                                 <  1:69789/141‑1 (MQ=255)
                                            gACAAAAGCAAAATTAAGCGTTTATAATTTATCTTTAGTAATTAGATAAGCGAGAGGGAAATGACATTTGCAGATAATTTAGTCATTATAGAAACGCCAAAAAAAAAAAAAACCATTGAAAAGTATTTAGGTAAGAAatat                                                                                                 <  2:344303/141‑1 (MQ=255)
                                              cAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATaa                                                                                               <  1:402274/141‑1 (MQ=255)
                                              aaaaaGCAAAATTGAGCGTTTATCATTTATCTTTAGTAATTAGATTATCGAGGGGGAAATGACATGGGCAGATAATTTAGTCATTGTTGAAGCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTATGTAAGAAATATaa                                                                                               <  2:401854/140‑1 (MQ=255)
                                               aaaaGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAATAAATATaaa                                                                                              <  1:878025/141‑1 (MQ=255)
                                               aaaaGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATaaa                                                                                              >  1:511574/1‑141 (MQ=255)
                                                 aaGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGt                                                                                            <  2:967836/141‑1 (MQ=255)
                                                  aGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGtt                                                                                           >  1:557519/1‑141 (MQ=255)
                                                  aGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGtt                                                                                           >  1:123486/1‑141 (MQ=255)
                                                     aaaaTTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTtata                                                                                        >  2:473568/1‑141 (MQ=255)
                                                      aaaTTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAg                                                                                       >  1:1163160/1‑141 (MQ=255)
                                                      aaaTTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGAGGAAATGACATTAGCAGATAATTTAGTCATGGTTGAATAGCCTGCAAAAACAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTaatgg                                                                                       >  2:587249/1‑139 (MQ=255)
                                                      aaaTTAAGCGTTTATCATCTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAATTTAAGTTAGGGAAGAAATATCAAGTTATAg                                                                                       <  1:880086/141‑1 (MQ=255)
                                                           aaGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTAAAGATTCa                                                                                  >  2:179716/1‑141 (MQ=255)
                                                                 ttATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGa                                                                            <  2:846274/141‑1 (MQ=255)
                                                                 ttATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGa                                                                            <  1:102259/141‑1 (MQ=255)
                                                                  tATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGac                                                                           <  1:386310/141‑1 (MQ=255)
                                                                   aTCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGaca                                                                          >  2:97246/1‑141 (MQ=255)
                                                                      atgtATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGt                                                                       <  2:58720/138‑1 (MQ=255)
                                                                      agtgagtgttAGTAATTAGCATAGCGAGCGGGAAATGACACTGGCAGATAATTTAGTCCTTGTTGAAGCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCACTGGGACACGt                                                                       <  2:983095/133‑1 (MQ=255)
                                                                          aTCTTTAGTAATTAGATTAGCGAGGGGGAAATGAAATAGGCAGAGAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCagg                                                                   >  1:525074/1‑140 (MQ=255)
                                                                           tCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGAAGATAATTTAGTCATTGTTGAATCGACTGCGGAATCAAAGCCCCATAAAAATTAGTGAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCagag                                                                  <  1:572209/141‑1 (MQ=255)
                                                                           tCTTTAGTAATTAGATGAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCagag                                                                  <  1:502339/141‑1 (MQ=255)
                                                                            cTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAgaga                                                                 <  2:740669/141‑1 (MQ=255)
                                                                              ttAGTAATTAGATTGGCGAGGGAGAAATGACATCAGCATATAAGTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACt                                                               <  2:956243/141‑1 (MQ=255)
                                                                                      tagattagCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGACTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGa                                                                            >  2:890744/1‑120 (MQ=255)
                                                                                      tagattagCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGACTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGa                                                                            <  1:890744/120‑1 (MQ=255)
                                                                                      tagattagCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGACTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGa                                                                            <  1:891829/120‑1 (MQ=255)
                                                                                      tagattagCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGACTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGa                                                                            >  2:891829/1‑120 (MQ=255)
                                                                                           tagCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGTCACGTCAGAGACTTACCAAGAAGTCa                                                  <  2:744300/141‑1 (MQ=255)
                                                                                             gCGAGGGGGAAATGACATTGGCAGATAATTTAGTGATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAATTATAGCTTCAATGGGAAACGTCAGAGAATTACCAAGAAGTaaaa                                                >  2:662490/1‑141 (MQ=255)
                                                                                              cGAGGGGGAAATGACATTGGCAGATAGTTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGAAACGTCAGAGACTTACCAAGAAGTCAAAt                                               <  1:760005/141‑1 (MQ=255)
                                                                                                 gggggAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATggg                                            >  2:1168573/1‑141 (MQ=255)
                                                                                                  ggggAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAAGCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGgt                                           >  1:721966/1‑141 (MQ=255)
                                                                                                  ggggAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGgt                                           <  2:537666/141‑1 (MQ=255)
                                                                                                      aaaTGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCg                                       <  1:213669/141‑1 (MQ=255)
                                                                                                           aCATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACt                                  >  1:498559/1‑141 (MQ=255)
                                                                                                           aCATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACt                                  <  1:552781/141‑1 (MQ=255)
                                                                                                            cATTGGTAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAACACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTg                                 <  1:927026/141‑1 (MQ=255)
                                                                                                              ttGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGaa                               >  1:1086239/1‑141 (MQ=255)
                                                                                                              ttGGCAGATAATTTAGTCATTGTTGAAACGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACAAAGAAGACAAATGGCTGTCGAACCTGaa                               >  2:354962/1‑141 (MQ=255)
                                                                                                               tGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTAACAAGAAGTCAAAAGGGTGACGACACTGAAg                              >  2:939124/1‑141 (MQ=255)
                                                                                                                ggCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGa                             <  2:1090579/141‑1 (MQ=255)
                                                                                                                   aGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATaa                          >  2:846532/1‑141 (MQ=255)
                                                                                                                      tAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTa                       <  1:151063/141‑1 (MQ=255)
                                                                                                                      tAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTa                       <  1:930456/141‑1 (MQ=255)
                                                                                                                       aaTTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTAc                      <  1:394473/141‑1 (MQ=255)
                                                                                                                       aaTTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTAc                      <  2:557519/141‑1 (MQ=255)
                                                                                                                       aaTTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGAGTCGTCACTGAAGATAATTAc                      <  1:611664/141‑1 (MQ=255)
                                                                                                                        aTTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGCCACTGAAGATAATTACg                     >  1:994390/1‑141 (MQ=255)
                                                                                                                        aTTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACg                     >  1:375786/1‑141 (MQ=255)
                                                                                                                         tttAGTCATTGTTGAATCGCCTGCAAAAGCAAACACCATTGAAAAGTATATAGGAAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAACTCAAATGTGTGTCGACACTGAAGATAATTACGa                    <  1:343352/141‑1 (MQ=255)
                                                                                                                          ttAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGaa                   <  2:952475/141‑1 (MQ=255)
                                                                                                                           tAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATAACGaaa                  >  2:748140/1‑140 (MQ=255)
                                                                                                                           tAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGATTCAATGGGGCGCGTCGGAGACTTAACAAGAAGTCAAATGGGTGTTGTCTTTGAAGATAATTACGacc                  >  1:321031/1‑139 (MQ=255)
                                                                                                                              tCATTGTTGAATCGCCTGCAAAAGAAACAACCATTGAAACGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTAACAAGAAGTCCACTGGAAGTCGACACTGAAGATAATTACGAACCaa               >  2:243895/1‑141 (MQ=255)
                                                                                                                                aTTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATGCCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTAAAGATAATTACGAACCaaaa             >  2:334889/1‑141 (MQ=255)
                                                                                                                                aTTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAACTGCGCGCCGACGCTGCAGATGATTACGAACCaaaa             >  2:1145782/1‑141 (MQ=255)
                                                                                                                                aTTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTAAGAGACTTACAAAAAAGTAAAATGGGTGTCGAAGCTGAAGATAATTAGGAACCaaaa             >  2:334420/1‑141 (MQ=255)
                                                                                                                                 ttgttgAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGCGGCGACACGGAAGATAATTACGAACCATAat            >  1:805109/1‑141 (MQ=255)
                                                                                                                                     tgAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGAACCAAAatatat        >  1:1027172/1‑141 (MQ=255)
                                                                                                                                     tgAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACAGAAGATAATTACGAACCAAAatatat        >  1:206401/1‑141 (MQ=255)
                                                                                                                                      gAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGAACCAAAAtatata       >  1:96380/1‑141 (MQ=255)
                                                                                                                                       aaTCGCCTGCAAAGGCAAAAACCATTGAAAAGTATGTAGGTAAGAAATATAAGGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGGAGTCAGATGGGTGTCGACACTAGAAATAAGTACGAAcagaataaagtaa      >  1:100055/1‑129 (MQ=255)
                                                                                                                                          cGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACAAAGAAGTCAAATGGGTGGCGACACTGAAGATAATTACGAACCAAAATAGATAACaa   >  2:618796/1‑141 (MQ=255)
                                                                                                                                           gCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGAACCAAAATATATAACAAt  <  1:635618/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TAAAAAATAGACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGATTAGCGAGGGGGAAATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGAACCAAAATATATAACAAT  >  CP000730/1266607‑1266886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑