Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,415,237 T→A 5.0% I92L (ATA→TTA)  USA300HOU_1312 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,415,2370TA 5.0% 289.7 / 3.6 91I92L (ATA→TTA) USA300HOU_1312hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/43);  new base A (3/2);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTCCATGTTCAATTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCAATATAACAACGATATACTT  >  CP000730/1415097‑1415377
                                                                                                                                            |                                                                                                                                            
ttCTCCATGTTCAATTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATtat                                                                                                                                              >  2:229131/1‑141 (MQ=255)
   tcCATGTTCAATTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATg                                                                                                                                           <  1:998297/141‑1 (MQ=255)
   tcCATGTTCAATTAACATGTGTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATg                                                                                                                                           >  1:727547/1‑141 (MQ=255)
     cttGTCCAATTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGtt                                                                                                                                         <  1:351252/139‑1 (MQ=255)
     cATGTTCAATTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGtt                                                                                                                                         <  1:253164/141‑1 (MQ=255)
       tGTTCAATTAACATGTTTAAATATTCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTAACCATCGCGACGTGTCAGCGTGTGCGTTAGTGTGCTTCAACTTCGTTTAACAGCATTATCTGtttt                                                                                                                                       >  2:542849/1‑140 (MQ=255)
            aaTTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATTTTTATTtgt                                                                                                                                  >  2:991308/1‑140 (MQ=255)
             aTTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTtgct                                                                                                                                 >  1:376186/1‑141 (MQ=255)
             aTTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTtgct                                                                                                                                 <  1:404476/141‑1 (MQ=255)
                aaCATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTgctgct                                                                                                                              <  2:1184879/141‑1 (MQ=255)
                  cATGTTTAAATATGCATCGCGGTGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTTGTTTAACATCATTATATGTTTATTTGCTGCTTc                                                                                                                            <  2:464741/141‑1 (MQ=255)
                  cATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTAAAGACTACCACTCCACATAGTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTc                                                                                                                            <  2:720111/141‑1 (MQ=255)
                    tGTTTAAATATTCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTg                                                                                                                          <  1:1038106/141‑1 (MQ=255)
                    tGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATGTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTg                                                                                                                          <  1:1037762/141‑1 (MQ=255)
                     gTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTgg                                                                                                                         <  1:1071995/141‑1 (MQ=255)
                               gCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTa                                                                                                               <  2:260332/141‑1 (MQ=255)
                               gCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTa                                                                                                               <  1:987348/141‑1 (MQ=255)
                                cATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTaa                                                                                                              <  2:726071/141‑1 (MQ=255)
                                cATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTaa                                                                                                              <  1:761180/141‑1 (MQ=255)
                                    gcgGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAagag                                                                                                          >  1:1190659/1‑141 (MQ=255)
                                     cgtaGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGt                                                                                                         <  2:78405/138‑1 (MQ=255)
                                     cgGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGt                                                                                                         <  1:111878/141‑1 (MQ=255)
                                      ggAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTATTGAATTTCGGTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTa                                                                                                        >  1:566455/1‑141 (MQ=255)
                                      ggAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGT‑ATTGATAATCTTCTTCAACTTCGTTTAACATCATTTTATGTTTATTTCATACATCTGGCCTAGCTGTAAAGCGTaa                                                                                                       >  2:212863/1‑141 (MQ=255)
                                        aGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGCGTAAt                                                                                                      >  1:998864/1‑141 (MQ=255)
                                        aGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAAt                                                                                                      >  1:768402/1‑141 (MQ=255)
                                         gTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATg                                                                                                     >  1:884863/1‑141 (MQ=255)
                                         gTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATg                                                                                                     >  2:78036/1‑141 (MQ=255)
                                         gTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTAAATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATg                                                                                                     >  1:87798/1‑141 (MQ=255)
                                         gTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTAAATATTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATg                                                                                                     >  1:218173/1‑141 (MQ=255)
                                            cttcttcACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCaa                                                                                                  >  1:882692/1‑141 (MQ=255)
                                             ttcttcACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAAt                                                                                                 <  1:292703/141‑1 (MQ=255)
                                             ttcttcACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAAt                                                                                                 <  1:916005/141‑1 (MQ=255)
                                              tcttcACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTTTTTGTTTATTGACTGCTTCTGGACTAGCTGTAAAGAGTAATGCAAtt                                                                                                >  1:326763/1‑140 (MQ=255)
                                              tcttcACTCATATCATTAATCATTTCTGTAAGACTATCAATCCTCAGATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTTGATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGGAATGCAATc                                                                                                <  2:770594/141‑1 (MQ=255)
                                                ttcACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCat                                                                                              <  2:1179553/141‑1 (MQ=255)
                                                 tcACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCatt                                                                                             >  2:132663/1‑140 (MQ=255)
                                                 tcACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCatt                                                                                             >  2:132962/1‑140 (MQ=255)
                                                   aCTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATg                                                                                           <  1:681061/141‑1 (MQ=255)
                                                   aCTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATAGATTTATTTGTTGCTTCTGGACTCCAGGTAAAGAGTAGTGCAATCATATg                                                                                           >  1:839041/1‑141 (MQ=255)
                                                     tCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGtt                                                                                         >  1:1147519/1‑141 (MQ=255)
                                                      cATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGttt                                                                                        <  2:339934/141‑1 (MQ=255)
                                                         atCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACa                                                                                     >  1:1075672/1‑141 (MQ=255)
                                                          tCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACat                                                                                    <  1:1050406/141‑1 (MQ=255)
                                                          tCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACat                                                                                    <  2:594466/141‑1 (MQ=255)
                                                          tCACTAATCATTTCTTTAAGACCATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACat                                                                                    <  2:301613/141‑1 (MQ=255)
                                                              tAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCa                                                                                <  2:111837/141‑1 (MQ=255)
                                                              tAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTGCGGTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCa                                                                                <  2:111979/141‑1 (MQ=255)
                                                                aTCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACt                                                                              <  2:188114/141‑1 (MQ=255)
                                                                  cATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCg                                                                            >  2:453002/1‑141 (MQ=255)
                                                                   aTTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGATTAATGCAATCATATGTTTACATATCACTCGt                                                                           <  2:727547/141‑1 (MQ=255)
                                                                   aTTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGt                                                                           <  1:552572/141‑1 (MQ=255)
                                                                   aTTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACCTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGt                                                                           <  1:1053985/141‑1 (MQ=255)
                                                                    tttCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCATCGTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTc                                                                          <  1:92127/141‑1 (MQ=255)
                                                                     ttCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTGATGCAATCATATGTTTACATATCACTCGTCt                                                                         >  2:430754/1‑141 (MQ=255)
                                                                     ttCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCt                                                                         >  1:1015411/1‑141 (MQ=255)
                                                                     ttCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCt                                                                         <  2:768402/141‑1 (MQ=255)
                                                                       ttttAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCACCTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTc                                                                       <  2:729776/140‑1 (MQ=255)
                                                                                 aTCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAATATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTAGTCTTCCATCAGCATg                                                             >  1:817809/1‑141 (MQ=255)
                                                                                 aTCAATCCACATATTTACGCGTAATTGATAGTCTTCTTCAAATTCGTTTAACATCCTTATATGTTTATTTACTGATTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCCCTCGTCTTCCATCAGCATg                                                             >  2:324133/1‑141 (MQ=255)
                                                                                    aaTCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAgg                                                          <  1:997842/141‑1 (MQ=255)
                                                                                      tCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTCCTGCTTCTGAACTAGCTGTCAAGACGAATGCAACCATATGTTTACATATCACTCTGCTTCCATCCGCATGAgggc                                                        >  2:810050/1‑139 (MQ=255)
                                                                                          tagatTTCTTCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAAtt                                                    <  2:710493/138‑1 (MQ=255)
                                                                                             atTTCTTCGTAATTGATAGTCTTCTTCAACTGCGTTTAACCTCATTATCTGTTGATTTGATGCTTCTGGACTAGCTTTAAAGAGTAATGCAATCATATGTTTACATCTCAATCGTCTTCCCTCAGCATGAGGTCAATTACa                                                 >  1:483690/1‑141 (MQ=255)
                                                                                              tttCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACat                                                >  1:68759/1‑141 (MQ=255)
                                                                                                    cctAATTGATAGTTTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGAt                                          <  2:606301/139‑1 (MQ=255)
                                                                                                    cGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGAt                                          <  1:736157/141‑1 (MQ=255)
                                                                                                      taattaatAGTCTTCTTCAACTTCGATTAACATCATTAACTGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAAGGCAATCATATGTTTACATACCACTCGTCTTCCATCAGCATCAGGACAATTACATATGGGttt                                        >  2:480663/1‑141 (MQ=255)
                                                                                                         ttGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCt                                     >  2:1166052/1‑141 (MQ=255)
                                                                                                         ttGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCt                                     >  1:802775/1‑141 (MQ=255)
                                                                                                         ttGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCt                                     <  2:959455/141‑1 (MQ=255)
                                                                                                          tGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTa                                    >  1:645413/1‑141 (MQ=255)
                                                                                                           gATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGAAAATTACATATGGATTTTCTAg                                   >  2:546698/1‑141 (MQ=255)
                                                                                                            acaatcttctTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAgg                                  >  2:437562/5‑141 (MQ=255)
                                                                                                            aTAGTCTTGTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTGTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTGACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAgg                                  >  2:882820/1‑141 (MQ=255)
                                                                                                             tAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGTACAATGACATTTGGATTTTCTAGGa                                 <  1:656270/141‑1 (MQ=255)
                                                                                                                tcttctTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCAGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGAGTTTCTACGATGt                              >  2:776550/1‑141 (MQ=255)
                                                                                                                  ttcttcAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTc                            >  2:1105406/1‑141 (MQ=255)
                                                                                                                   tcttcAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTcc                           >  1:645169/1‑141 (MQ=255)
                                                                                                                    cttcAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTcct                          >  1:594744/1‑140 (MQ=255)
                                                                                                                    cttcAACTTCGTTTAACATCATTAAATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCa                          <  2:218173/141‑1 (MQ=255)
                                                                                                                    cttcAACTTCGTTTAACATCATTAAATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCa                          <  2:87798/141‑1 (MQ=255)
                                                                                                                      tcAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCata                        <  2:884863/141‑1 (MQ=255)
                                                                                                                         aCTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCa                     >  1:37342/1‑141 (MQ=255)
                                                                                                                         aCTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTATTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCa                     <  1:801020/141‑1 (MQ=255)
                                                                                                                          cTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCaa                    >  1:642328/1‑141 (MQ=255)
                                                                                                                              gTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATCTGTTTACATATCACTCTTCTTCCATCAGCATGAGGCCAATTAAATATGGATTTTTTAGGANGTTCGATATCCTtata                >  1:937446/1‑141 (MQ=255)
                                                                                                                                 tAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCAATATaaca             <  2:66327/141‑1 (MQ=255)
                                                                                                                                   aCATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGGTGTTCCATATTAATATaacaac           >  1:999424/1‑141 (MQ=255)
                                                                                                                                   aCATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCAATATaacaac           >  1:1052808/1‑141 (MQ=255)
                                                                                                                                     atcatTATATGTTTATTTGATGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGACTTTCTCGGATGTTCCATATCAATATAACAACgt         >  2:667254/1‑140 (MQ=255)
                                                                                                                                       catTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCAATATAACAACGata       <  1:279036/141‑1 (MQ=255)
                                                                                                                                           atatGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGCATAGTACATATGGATTTTCTAGGATGTTCCATATCAATATAACAACGATATACt   <  1:693365/141‑1 (MQ=255)
                                                                                                                                            tatGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCAATATAACAACGATATACtt  >  2:198599/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TTCTCCATGTTCAATTAACATGTTTAAATATGCATCGCGGAGTTCTTCTTCACTCATATCATTAATCATTTCTTTAAGACTATCAATCCACATATTTCTGCGTAATTGATAGTCTTCTTCAACTTCGTTTAACATCATTATATGTTTATTTGCTGCTTCTGGACTAGCTGTAAAGAGTAATGCAATCATATGTTTACATATCACTCGTCTTCCATCAGCATGAGGACAATTACATATGGATTTTCTAGGATGTTCCATATCAATATAACAACGATATACTT  >  CP000730/1415097‑1415377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑