Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,551,935 G→T 5.6% S180* (TCA→TAA)  ebpS ← elastin‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,551,9350GT 5.6% 326.9 / 2.1 108S180* (TCA→TAA) ebpSelastin‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (48/51);  new base T (6/2);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTCTTCG  >  CP000730/1551795‑1552075
                                                                                                                                             |                                                                                                                                            
cTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTg                                                                                                                                              >  2:1153895/1‑141 (MQ=255)
 tttAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGa                                                                                                                                             >  1:213761/1‑141 (MQ=255)
  ttAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTCTGAAGGTGTTGGTTTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGAt                                                                                                                                            >  1:566181/1‑141 (MQ=255)
   tAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTTAtt                                                                                                                                           >  2:960815/1‑140 (MQ=255)
    aGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGAGGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGt                                                                                                                                          <  2:143357/141‑1 (MQ=255)
      aaGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGttt                                                                                                                                        >  2:215984/1‑141 (MQ=255)
          agcCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTCCAGAATCATTATCATGTTCATTTTTTGATGTTTCTgt                                                                                                                                    >  2:175723/1‑140 (MQ=255)
          agcCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGa                                                                                                                                    >  1:300994/1‑141 (MQ=255)
           gcCATTGCACCCGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTTATGTTTCTGAt                                                                                                                                   <  2:94688/141‑1 (MQ=255)
           gcCATTGCACCAGCTGCACCTGCAACACCTTCTGTTCCATCACACTTGTCCCGTTCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGAt                                                                                                                                   <  1:865256/141‑1 (MQ=255)
            ccATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTTTTTTTTTGctgtttctgttt                                                                                                                                  >  2:776152/1‑141 (MQ=255)
             cATTGCCTCAGCTACTCTGGCAACACCTGCCGTTCCAGCACTGTTTGCTGCTGCTTTTTTACCATTATGATGTTCTTTATGTTCATCTTTATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTg                                                                                                                                 <  2:66222/141‑1 (MQ=255)
             cATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTCAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTg                                                                                                                                 >  1:807567/1‑141 (MQ=255)
               ttGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGt                                                                                                                               >  2:76793/1‑141 (MQ=255)
               ttGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGt                                                                                                                               >  2:224497/1‑141 (MQ=255)
                   aCCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCAGTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGc                                                                                                                           >  1:1135568/1‑141 (MQ=255)
                     cAGCTGCCCCTGCAACTCCGGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCAc                                                                                                                         <  2:951941/141‑1 (MQ=255)
                           cACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTCGGTTCATCTTGATCTTGTTTTACAGAATCATTATCCTGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCtgttt                                                                                                                   >  2:1091342/1‑140 (MQ=255)
                            aCCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTTATTTTTCTGATTGGTTAGCAGCtgttgt                                                                                                                  >  2:384209/1‑141 (MQ=255)
                              cTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTaa                                                                                                                <  2:213761/141‑1 (MQ=255)
                              cTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTaa                                                                                                                >  2:632954/1‑141 (MQ=255)
                               tGCAACCCCTGCTGGTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTaaa                                                                                                               <  1:788219/141‑1 (MQ=255)
                                     cacgcgtgTTCCAGCACCAATAGCTGCTGCTTTTTTACGATTTTAATGTTCTTTAGGTTCATCTTGCTCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTGCTGATTGGTGAGCACCTGTTGTAAAATATgg                                                                                                         <  2:45713/135‑1 (MQ=255)
                                     acCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTAATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATgg                                                                                                         >  2:44174/1‑141 (MQ=255)
                                      ccTGCTGTTCCAGCACCAGTAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGt                                                                                                        <  2:133238/141‑1 (MQ=255)
                                        tgctgTTCCAGCACCAATCGCTGCTGTTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGttt                                                                                                      <  2:92651/141‑1 (MQ=255)
                                          ctgTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTCCAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGtttcg                                                                                                    >  2:412539/1‑139 (MQ=255)
                                          ctgTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAg                                                                                                    >  1:926261/1‑141 (MQ=255)
                                              tCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTg                                                                                                <  1:972091/141‑1 (MQ=255)
                                                cAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCCCCTGTTGTAAAATATGGTTTAGGTTGCt                                                                                              >  2:332913/1‑141 (MQ=255)
                                                 aGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTg                                                                                             >  2:1072504/1‑141 (MQ=255)
                                                  gCACCAATAGCTGCTGCTTTTTTACCCTTATGATGTTCTTTAGGTTCATCTTGCTCTTTTTTTACATAATCATTATCATGTTCATTTTTTGATGTTTCTGCTTGGCTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTgt                                                                                            >  2:298151/1‑140 (MQ=255)
                                                         tAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGAACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGtt                                                                                     >  2:915057/1‑141 (MQ=255)
                                                           gctgctgctTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCa                                                                                   <  1:1115796/141‑1 (MQ=255)
                                                            ctgctgctTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAg                                                                                  <  1:1078988/141‑1 (MQ=255)
                                                              gctgctTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTTATGTTTTTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGGGTTTGTTGAggt                                                                                >  2:518929/1‑139 (MQ=255)
                                                              gctgctTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCt                                                                                <  2:750826/141‑1 (MQ=255)
                                                              gctgctTTTTTACCATTATGATGTGCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAACATATGGTTTAGGTTGCTGAGATTGTTCAGCt                                                                                >  1:767144/1‑141 (MQ=255)
                                                                tgctTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTc                                                                              <  2:833095/141‑1 (MQ=255)
                                                                    tttttACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTTTTTTTTTTTATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCActc                                                                          >  2:1038444/1‑141 (MQ=255)
                                                                    tttttACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCActc                                                                          >  1:565024/1‑141 (MQ=255)
                                                                    tttttACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCActc                                                                          <  2:943538/141‑1 (MQ=255)
                                                                    tttttACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCActc                                                                          >  2:417669/1‑141 (MQ=255)
                                                                          ccacattgacgTTCTTTCGGTTCATCGTGATCTTGTTTTACCGACTCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCa                                                                    <  2:64064/135‑1 (MQ=255)
                                                                          ccATCATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCa                                                                    <  1:299914/141‑1 (MQ=255)
                                                                           cATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAg                                                                   <  2:825547/141‑1 (MQ=255)
                                                                            aTTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGa                                                                  <  1:1020522/141‑1 (MQ=255)
                                                                            aTTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGa                                                                  >  1:790736/1‑141 (MQ=255)
                                                                               atgatgTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAAc                                                               <  1:1167986/141‑1 (MQ=255)
                                                                               atgatgTTCTTTAGGTGCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAAc                                                               <  2:100559/141‑1 (MQ=255)
                                                                                 gatgTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTg                                                             >  1:969841/1‑141 (MQ=255)
                                                                                 gatgTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTg                                                             >  1:580805/1‑141 (MQ=255)
                                                                                  atgTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGt                                                            <  2:1008886/141‑1 (MQ=255)
                                                                                  atgTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGt                                                            <  2:394843/141‑1 (MQ=255)
                                                                                    gTTCTTTAGGTTCATCTTGATCTTGTTTTCTTAATGCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTg                                                          >  1:577004/1‑141 (MQ=255)
                                                                                     ttCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGa                                                         <  2:203962/141‑1 (MQ=255)
                                                                                     ttCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGa                                                         <  2:606349/141‑1 (MQ=255)
                                                                                       cTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAAt                                                       >  1:785465/1‑141 (MQ=255)
                                                                                        tttAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATg                                                      <  2:1076208/141‑1 (MQ=255)
                                                                                          tAGGTTCATCTTGATCTTGTTTTACAGAATCGTGATGATGTTCATTTTTTGATTTTTCGGATTTGTTAGGACCTGTTGTAAAATATAGTTTATGTTGCGAAGTTTGTTCAGCTTCACGCTTCTCAGGAACAGtatcaggcg                                                    >  2:71735/1‑133 (MQ=255)
                                                                                          tAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCt                                                    >  2:360788/1‑141 (MQ=255)
                                                                                             gTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAg                                                 >  1:581851/1‑141 (MQ=255)
                                                                                             gTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTACACTCTTATCAGAAACTGTTGAATGCTCAg                                                 >  1:1141569/1‑141 (MQ=255)
                                                                                               tCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAgtg                                               <  1:375367/141‑1 (MQ=255)
                                                                                                  tcttgatcttgTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGACTGCTCAGTGttt                                            >  2:299560/1‑140 (MQ=255)
                                                                                                  tcttgatcttgTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTa                                            <  1:215984/141‑1 (MQ=255)
                                                                                                      tatcttgTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGGTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGGATGCTCAGTGTTAtttt                                        <  2:234979/140‑1 (MQ=255)
                                                                                                      gattttgTCGTACAGAATGATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTAtttt                                        <  2:122898/141‑1 (MQ=255)
                                                                                                      gatcttgTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTAtttt                                        >  1:207770/1‑141 (MQ=255)
                                                                                                      gatcttgTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTAtttt                                        <  1:262680/141‑1 (MQ=255)
                                                                                                      gatcttgTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTAtttt                                        >  2:1100799/1‑141 (MQ=255)
                                                                                                       atcttgTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTc                                       <  2:999014/141‑1 (MQ=255)
                                                                                                          ttgTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGc                                    <  2:271471/141‑1 (MQ=255)
                                                                                                          ttgTTTTACAGAATCATTATCATGTTCATTTTGTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGc                                    <  2:841312/141‑1 (MQ=255)
                                                                                                                tACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCAttttt                              >  2:102512/1‑141 (MQ=255)
                                                                                                                    gAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATa                          <  1:895200/141‑1 (MQ=255)
                                                                                                                      aTCATTATCATGTTCATTTTTTTTTTTTTTTTATTGGTTAGCACCTGTTGTAAAATAAGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGTTTTTTTAATAGt                        >  2:156674/1‑141 (MQ=255)
                                                                                                                      aTCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGt                        >  1:705519/1‑141 (MQ=255)
                                                                                                                      aTCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGt                        >  2:63508/1‑141 (MQ=255)
                                                                                                                      aTCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGt                        >  2:786204/1‑141 (MQ=255)
                                                                                                                      aTCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTACAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGt                        <  1:518929/141‑1 (MQ=255)
                                                                                                                            tATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCgt                   >  1:1163962/1‑141 (MQ=255)
                                                                                                                             ttCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCCCCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCgtg                  <  2:451043/140‑1 (MQ=255)
                                                                                                                               cATGTTCATTTTTTGATGTCACGGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTCATAGTATCGTGtt                >  1:881374/1‑141 (MQ=255)
                                                                                                                                 tGTTCATTTTTTTTTTTTTTTTATTGCTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTTTTGAATTTTCATTTTTTTTTTTTTTTTTTTTATTAGTATCGTGtttt              >  2:445506/1‑140 (MQ=255)
                                                                                                                                 tGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTa              <  1:277105/141‑1 (MQ=255)
                                                                                                                                     cATTTTTTGATGTTTCTGATTTGTTAGCACCTGTTGTCAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGATTGCTGAGTGTTATTTTCTGCATGATTCATATTATCGTGTTTACCAt          >  2:562621/1‑141 (MQ=255)
                                                                                                                                     cATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCAt          >  1:796564/1‑141 (MQ=255)
                                                                                                                                     cATTTTATGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGGTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATACTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCAt          >  2:567632/1‑141 (MQ=255)
                                                                                                                                      aTTTTTTTGTTTTTCTGATGGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGGGTTTATCAtt         <  2:602188/141‑1 (MQ=255)
                                                                                                                                       ttttttGATGTTTCTTATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTCCTAGTATCGTGTTTCTCATTg        <  2:917576/141‑1 (MQ=255)
                                                                                                                                        tttttGTTGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGt       <  2:300799/141‑1 (MQ=255)
                                                                                                                                        tttttGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGt       <  1:482520/141‑1 (MQ=255)
                                                                                                                                         ttgtGATGGTGCTGATTGGTCAGCACCTGTTGTAACATAGGGTTTAGGTTGCTGGGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTc      <  1:497027/138‑1 (MQ=255)
                                                                                                                                          tttGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCACCTTCACTCTTATCAGGAACCGTTGCAGGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTCt     <  1:636756/141‑1 (MQ=255)
                                                                                                                                          tttGATGTTTCGGATTGGTTCGCCCCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTCt     <  1:1022730/141‑1 (MQ=255)
                                                                                                                                           ttGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTCtt    <  1:1072504/141‑1 (MQ=255)
                                                                                                                                             gATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTCTTct  >  2:1178197/1‑140 (MQ=255)
                                                                                                                                             gATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTCTTCg  >  1:71962/1‑141 (MQ=255)
                                                                                                                                             gATGTTTCTGATTGGTTAGCACCTGTGGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTCTTCg  >  2:1187219/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                            
CTTTAGAAGCAGCCATTGCACCAGCTGCACCTGCAACACCTGCTGTTCCAGCACCAATAGCTGCTGCTTTTTTACCATTATGATGTTCTTTAGGTTCATCTTGATCTTGTTTTACAGAATCATTATCATGTTCATTTTTTGATGTTTCTGATTGGTTAGCACCTGTTGTAAAATATGGTTTAGGTTGCTGAGATTGTTCAGCTTCACTCTTATCAGAAACTGTTGAATGCTCAGTGTTATTTTCTGCATTTTTAATAGTATCGTGTTTATCATTGTCTTCG  >  CP000730/1551795‑1552075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: