Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,646,1890GC100.0% 334.3 / NA 98A155G (GCA→GGA) USA300HOU_1531M24B family Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (57/41);  total (57/41)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAC  >  CP000730/1646053‑1646324
                                                                                                                                        |                                                                                                                                        
tttATCACTTGCAACACCATGTGGTAATGGACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGACTTTATTTCTTTTTTTGTCATGCCTCCttt                                                                                                                                      >  1:321427/1‑141 (MQ=255)
 ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATACCTCCtttt                                                                                                                                     >  2:761007/1‑141 (MQ=255)
 ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAAACGTATCGAATGATGTTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                     >  2:529219/1‑141 (MQ=255)
  tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                   >  2:271714/1‑141 (MQ=255)
   ttCACTTGCAGTACCGTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                  <  2:988688/140‑1 (MQ=255)
   aTCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                  >  1:357874/1‑141 (MQ=255)
   aTCACTTGCAACACCATGTGGTAATACACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                  >  2:1060083/1‑141 (MQ=255)
    tCACTTGCAACACCATGTGGTAATGGACCCCTATGACCAGATGCTACAATCGTATCGGATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTACCTTTAATTGTTTTTCAGTCATGCCTCCTTTTact                                                                                                                                 >  2:683928/1‑140 (MQ=255)
       cTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaaca                                                                                                                              >  2:1059102/1‑141 (MQ=255)
        ttGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAg                                                                                                                             <  1:364272/141‑1 (MQ=255)
            aaCACCATGTGGTAATGCACCTCTAAAACAAGATGCTACAATCGTATCTAATGCTGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTACCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                                         >  2:529217/1‑141 (MQ=255)
              ctccATGTGGTAATGCACCTCTATGGCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATCTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                       <  1:935132/139‑1 (MQ=255)
              cacCTTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                       <  2:649263/141‑1 (MQ=255)
              cacCATGTGGTAATGCACCTCTATGCCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                       <  2:649676/141‑1 (MQ=255)
                   tgtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatatat                                                                                                                  >  2:945944/1‑141 (MQ=255)
                    gtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                                                                                 <  2:275815/141‑1 (MQ=255)
                     tgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTc                                                                                                                >  1:256018/1‑141 (MQ=255)
                      ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCa                                                                                                               <  1:415708/141‑1 (MQ=255)
                      ggTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCa                                                                                                               >  1:271963/1‑141 (MQ=255)
                       gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                              <  1:300346/141‑1 (MQ=255)
                           tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGt                                                                                                          <  1:758954/141‑1 (MQ=255)
                             cACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGttt                                                                                                        <  1:252245/141‑1 (MQ=255)
                              aCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtc                                                                                                       <  1:662523/141‑1 (MQ=255)
                                ctctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtctt                                                                                                     >  1:485574/1‑139 (MQ=255)
                                           gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTTATTCTTTTTCAGTCATGCATCCTTTTGCAACAGTTAAAATATATTCATATGTTTTATCAACAATATTa                                                                                          >  2:395865/1‑141 (MQ=255)
                                             tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATTCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgc                                                                                        >  2:34955/1‑141 (MQ=255)
                                               cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATCTTAgctg                                                                                      >  2:266555/1‑141 (MQ=255)
                                               cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                                                                      <  2:1016373/141‑1 (MQ=255)
                                                tACAAGCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTCACCCCATTGAATGAGCTTCATATGTTTCATCAACAATATTAgctgc                                                                                     >  2:539947/1‑141 (MQ=255)
                                                 aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgct                                                                                    >  2:24488/1‑141 (MQ=255)
                                                  cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCCGTCATGCCCCCTTTTACAACAGTTAAAATATATTCGTATGTTTCTTCAACAATATTAGCTGCtt                                                                                   <  1:780457/141‑1 (MQ=255)
                                                  cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                                   <  1:700215/141‑1 (MQ=255)
                                                  cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                                                                   >  1:288132/1‑141 (MQ=255)
                                                  agtactaagcGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTAATATGTTTCATCAACAATATTAGCTGCtt                                                                                   >  2:309554/10‑141 (MQ=255)
                                                    aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                 <  1:673328/141‑1 (MQ=255)
                                                    aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                 <  1:1019574/141‑1 (MQ=255)
                                                      cGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCCACAATATTAGCTGCTTTTTg                                                                               >  2:1184710/1‑141 (MQ=255)
                                                         aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                            >  1:124721/1‑141 (MQ=255)
                                                         aTCGAATGATGGTCCATCTACTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAGTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATCTGTTTCATCAACACTATTAGCTGCTTTTTGAAt                                                                            >  2:667000/1‑141 (MQ=255)
                                                          tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                           <  1:837818/141‑1 (MQ=255)
                                                           ctaaTGATGGTCCATCTGCTCCTAATTCTATCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTa                                                                          <  1:230923/139‑1 (MQ=255)
                                                              atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                       >  1:670356/1‑141 (MQ=255)
                                                              atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                       >  2:276445/1‑141 (MQ=255)
                                                               tgatgGTCCATCTGCTCCTAATTCTTGCATTTTGCTTTCTAATATTTCCTTTAATTCTGTTGCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATCGTTTATCAACAATATTAGCTGCTTTTTGAATTAAAGc                                                                      <  2:787395/141‑1 (MQ=255)
                                                               tgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTGTCTAATATTGCCTTTAATTCTTTTTCTGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGc                                                                      <  1:809526/141‑1 (MQ=255)
                                                                gatgGTCCATCTGCTCCTAATTCTAGCTTTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTGTTAGAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCa                                                                     <  2:682649/141‑1 (MQ=255)
                                                                 atgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGACTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATGACCAATGTTAGCTGCTTTTTGAATTAAGGCaa                                                                    >  1:398669/1‑141 (MQ=255)
                                                                 atgGTCCATCTGCTCCTAATTCTAGCATTTTGATTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCaa                                                                    >  1:856913/1‑141 (MQ=255)
                                                                  tgttCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAACATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAt                                                                   <  2:933003/138‑1 (MQ=255)
                                                                   ggtctactttcTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                  <  2:1140891/131‑1 (MQ=255)
                                                                    gTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAttt                                                                 >  2:99065/1‑141 (MQ=255)
                                                                        ttCTGCTCCTAATTATAGCCTTTTGCCTTATAATCTTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                             <  1:539947/140‑1 (MQ=255)
                                                                         tCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCa                                                            >  2:593160/1‑141 (MQ=255)
                                                                           tGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACATTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCACTTTCGTCCGc                                                          >  1:570904/1‑141 (MQ=255)
                                                                             cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCCTCAGCAt                                                        <  1:751633/141‑1 (MQ=255)
                                                                             cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTCTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCGGCAt                                                        >  1:828786/1‑141 (MQ=255)
                                                                              tCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATc                                                       >  1:452947/1‑141 (MQ=255)
                                                                                cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCatgatcgt                                                     >  2:737220/1‑134 (MQ=255)
                                                                                 tAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                    <  2:356205/141‑1 (MQ=255)
                                                                                 tAATTCTAGCATTTTGCTTTCTAATATTGCCTTCAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAACttt                                                    <  1:812690/141‑1 (MQ=255)
                                                                                      cTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                               <  1:402043/141‑1 (MQ=255)
                                                                                         gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTACAGCAATTTCGTCAGCATCTTTGACGtctc                                            >  1:730845/1‑141 (MQ=255)
                                                                                             tttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAt                                        <  1:24488/141‑1 (MQ=255)
                                                                                              ttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTACtt                                       >  2:1123368/1‑141 (MQ=255)
                                                                                              ttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                       <  1:1060083/141‑1 (MQ=255)
                                                                                                  tttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCGCTAATTTTAt                                   >  2:59438/1‑141 (MQ=255)
                                                                                                    tCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCt                                 <  2:324197/141‑1 (MQ=255)
                                                                                                      tAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCCTATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTaa                               >  2:197415/1‑140 (MQ=255)
                                                                                                       aaTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTAcc                              >  2:5358/1‑140 (MQ=255)
                                                                                                       aaTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTACTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCAAATTTTATCTACa                              >  2:321252/1‑141 (MQ=255)
                                                                                                         tatttactatAATTCTTTTTCAGTCATGCCTCCTTTTACAACCGTTAAAATATATACATATGTTTCATCAACAATATTAGCTTCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGt                            <  1:395865/132‑1 (MQ=255)
                                                                                                         tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGt                            <  1:981883/141‑1 (MQ=255)
                                                                                                          atTGCCTTTAATTCTTTTTCCGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGtt                           >  1:1005630/1‑140 (MQ=255)
                                                                                                          atTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTCAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTTGTCAGCATCTTTGGCGACTCTAATTTTATCTACAGTa                           >  1:161789/1‑141 (MQ=255)
                                                                                                            tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATTTGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                         >  2:8292/1‑141 (MQ=255)
                                                                                                            tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                         >  1:137506/1‑141 (MQ=255)
                                                                                                            tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                         <  2:532289/141‑1 (MQ=255)
                                                                                                               cTTTAATTCTTTTTCAGTCATGCCTCCTTTTAAACTTCGTGAAAAAAATTCTTTCTTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGa                      >  1:280898/1‑141 (MQ=255)
                                                                                                                ttttATTCTTTTTCAGTCATGCTTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTACTTTTATCTACAGTATTAGaa                     <  1:529219/141‑1 (MQ=255)
                                                                                                                tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa                     <  1:761007/141‑1 (MQ=255)
                                                                                                                    aTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                 >  2:501444/1‑141 (MQ=255)
                                                                                                                    aTTCTTTTTCAGTCATGACTCCTTTTACAAAAGTTAAAATATATTCCTCTGTTTCATAAAGAATCTTAGCTGATTTTTGAATTCAAGAATTTTAGTCAGCATAGTTGACGTCTCTAATTTTATCTACCGAATTATAAAtac                 >  2:211619/1‑139 (MQ=255)
                                                                                                                       cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta              >  1:970746/1‑141 (MQ=255)
                                                                                                                       cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAGGCtta              >  1:970500/1‑141 (MQ=255)
                                                                                                                        tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat             >  2:885311/1‑141 (MQ=255)
                                                                                                                        tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGAAGTCTCTAATTTTATCTACAGTATTAGAAATGCttat             >  2:691413/1‑141 (MQ=255)
                                                                                                                         ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGCCGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt            <  1:34955/141‑1 (MQ=255)
                                                                                                                         ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt            >  2:765126/1‑141 (MQ=255)
                                                                                                                         ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTCATCTACAGTATTAGAAATGCttatt            >  1:1052470/1‑141 (MQ=255)
                                                                                                                         ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta           >  1:597999/1‑141 (MQ=255)
                                                                                                                            tCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAACGACATTTCGGCATCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAAt         <  1:1123368/141‑1 (MQ=255)
                                                                                                                             cAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg        <  1:271714/141‑1 (MQ=255)
                                                                                                                              aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGa       <  2:729833/141‑1 (MQ=255)
                                                                                                                               gTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGat      >  2:643461/1‑141 (MQ=255)
                                                                                                                                 cATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAGTGCTTATTAATGatat    >  1:508191/1‑141 (MQ=255)
                                                                                                                                  aTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGAtata   >  1:1041869/1‑141 (MQ=255)
                                                                                                                                   tGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc  >  1:450705/1‑141 (MQ=255)
                                                                                                                                   tGCCTCCTTTTACAACAGTTAAAATATATTCAGTTCTTTCATCCACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGTATCTTTGACGTCTCTTATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc  <  2:265226/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAC  >  CP000730/1646053‑1646324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: